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... codons in the leader peptide  If the tryptophan levels are low, the ribosome stalls here on the leader peptide at the tryptophan codons, allowing the formation of the 2,3 antiterminator hairpin.  In the 5’ end of this transcript there are four stems that can form three different stem loops. o 1,2; ...
Outline for Unit 1 Solutions, Acid/Base, and Gases
Outline for Unit 1 Solutions, Acid/Base, and Gases

... Strong vs. Weak Bases: Strong base: completely (100%) dissociates into hydroxide (OH-) ions when dissolved in water. All oxides and hydroxides of the alkali metals and alkaline earths – group 1 and 2 are strong bases. (some are not very soluble in water but what does dissolve will ...
C h e m g u id e   –... DNA:  PROTEIN SYNTHESIS
C h e m g u id e –... DNA: PROTEIN SYNTHESIS

... 1. a) The tRNA molecule has to attach to the mRNA molecule where the mRNA has the code for methionine. In order for that to happen, the sequence of bases at the bottom of the tRNA molecule must be exactly complementary to those in the mRNA. The mRNA code for methionine is AUG. The anti-codon UAC wil ...
Chemistry 30 - SharpSchool
Chemistry 30 - SharpSchool

... Jill Agnew ...
Paper I- Discussion Points
Paper I- Discussion Points

... To establish the experimental set up that we need, let us move the green locus to the other side of the origin so that the distance between the origin and the red locus is the same as the distance between the red and the green loci. Or, the green locus is now twice as far away from the origin as the ...
DNA Analysis is our Ally
DNA Analysis is our Ally

... Many SNPs in many genes • DNA arrays • One multiplex PCR • Fluorescent read-out for each SNP of interest • Interpretation by software • Results ~ 5 hours (+ time to extract DNA) ...
Amino Acids and Simple Proteins
Amino Acids and Simple Proteins

... teach in higher education and the management of research projects students, the ability to provide teaching material in verbal, written and graphic form(PC-3) As a result of study a postgraduate students have: To know: physical and chemical nature of the processes occurring in living organisms at th ...
Identification and removal of colanic acid from plasmid DNA
Identification and removal of colanic acid from plasmid DNA

... and have been shown to cause significant immune responses in animals.17 Investigators have reported inflammation and significant immune responses in animals after intramuscular injections of naked DNA,18 and the levels of these immune responses were identical to those produced after intramuscular in ...
pdf of article - ACG Publications
pdf of article - ACG Publications

... metabolites. This fact is not surprising, since amino acids are soluble in polar solvents; therefore, the use of water associated with high temperatures enables their extraction. Alanine was clearly the major compound in both extracts, accounting for more than 83% of the total of amino acids (Table ...
Multifractal analysis of DNA sequences using a novel chaos
Multifractal analysis of DNA sequences using a novel chaos

... two of them on the 1=f spectrum of DNA sequences [3]. By mapping the sequence onto a (1D) walk, Peng and others have built a kind of interface, whose statistics were used to probe the range of correlation of the sequences [4,5]. Linguistic features were claimed to have been found in noncoding DNA s ...
Chapter 10
Chapter 10

... into fragments of the desired size by physical means, by treatment with restriction enzymes that have infrequent cleavage sites (for example, enzymes such as NotI and SfiI), or by treatment with ordinary restriction enzymes under conditions in which only a fraction of the restriction sites are cleav ...
Workshop: Protein Structure Introduction Learning Objectives
Workshop: Protein Structure Introduction Learning Objectives

... Proteins that have quaternary structure are formed from two or more polypeptides that assemble into one active structure. The different polypeptides in a protein with quaternary structure are often called subunits. These subunits may be identical or different. One example of a protein with quaternar ...
From DNA to Protein Structure and Function - Science Take-Out
From DNA to Protein Structure and Function - Science Take-Out

... this worksheet represents a small part of the DNA code in a gene that carries instructions for making one kind of protein. ...
Analytical Chemistry
Analytical Chemistry

... 1. Electrolytes: Electrolytes are solutes which ionize in a solvent to produce an electrically conducting medium. Strong electrolytes ionize completely whereas weak electrolytes are only partially in the solvent. Table 1: Strong electrolytes 1.the inorganic acids HNO3,HClO4,H2SO4,HCl,HI, HBr,HClO3,H ...
Course Outline
Course Outline

... are only about 80 nm. Most viruses are unable to be seen with a light microscope so scanning and transmission electron microscopes are used to visualise virus particles. To increase the contrast between viruses and the background, electron-dense "stains" are used. These are solutions of salts of he ...
Mutations and Genetic Variability 1. What is occurring in the diagram
Mutations and Genetic Variability 1. What is occurring in the diagram

Complete Mitochondrial DNA Sequence and Amino Acid Analysis of
Complete Mitochondrial DNA Sequence and Amino Acid Analysis of

... On the nucleotide level, the A þ T percentage for the A. aegypti COI gene is 68.6%. This high A þ T content falls within the mean values of 68.1 –70.7% reported for six species of nematoceran and brachyceran Diptera (Lunt et al., 1996). The codon usage in AeCOI, as seen in Table I, clearly shows a p ...
Chapter 11 - Nucleic Acid Structure and DNA Replication
Chapter 11 - Nucleic Acid Structure and DNA Replication

... To determine if DNA is the genetic material of T2, Hershey and Chase devised a method to separate the phage coat, which is attached to the outside of the bacterium, from the genetic material, which is injected into the cytoplasm. They reasoned that the attachment of T2 on the surface of the bacteriu ...
(THCA) synthase gene in
(THCA) synthase gene in

... acids (isoleucine, arginine and proline) as those in the other ‘‘drug-type’’ strains. The substitution at 187 bp was nonsynonymous: the substituted nucleotide C coded for leucine in #001 and for isoleucine in another ‘‘drug-type’’ strain. However, these two amino acids have the same chemical charact ...
Fatty acid synthesis
Fatty acid synthesis

... mixed acidic anhydride is formed ...
Biochemistry Lecture 4 9/6/01
Biochemistry Lecture 4 9/6/01

... strong acid/base ...
Chapter 16 Aqueous Ionic Equilibrium Lecture Presentation
Chapter 16 Aqueous Ionic Equilibrium Lecture Presentation

... • It is a plot of pH versus the amount of added titrant. • The inflection point of the curve is the equivalence point of the titration. • Prior to the equivalence point, the known solution in the flask is in excess, so the pH is closest to its pH. • The pH of the equivalence point depends on the pH ...
Identification of DNA polymorphism in cultivars using RAPD and AFLP
Identification of DNA polymorphism in cultivars using RAPD and AFLP

... obtained can vary considerably between different runs of the same sample. Even within a laboratory which may have different PCR machines or use different sources of polymerase and associated buffers. Even within a laboratory, the item saved by the direct application of RAPDs is often lost in achievi ...
Molecular cloning and nucleotide sequence of another variant of the
Molecular cloning and nucleotide sequence of another variant of the

... Bacteriophage induction. Induction of bacteriophages was accomplished by: (1) exposure of cultures to ultraviolet light as described by O'Brien e f al. (1984); and (2) addition of mitomycin C (Sigma) to midexponential-phase cultures (ODbo00.5) to a final concentration of 1 pg ml-I (Karch & Bitzman, ...
Testing Methylation Pathways
Testing Methylation Pathways

... added to specific elements of DNA, our gene markers and proteins that keep them physiologically active. Methylation is a major pathway to focus on in understanding autoimmune and neurological diseases such as multiple ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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