Lecture 2: Biology Review II
... Definition: A genetic map is an ordering of genes and markers in a linear arrangement corresponding to their physical order along the chromosome. Based on linkage. Definition: A physical map is an ordering of landmarks on DNA, regardless of inheritance. Measured in base pairs. ...
... Definition: A genetic map is an ordering of genes and markers in a linear arrangement corresponding to their physical order along the chromosome. Based on linkage. Definition: A physical map is an ordering of landmarks on DNA, regardless of inheritance. Measured in base pairs. ...
ap: chapter 16: the molecular basis of inheritance
... 1. After Morgan and fellow scientists developed the Chromosomal Theory of Inheritance, the search was on for the chemical mechanism of inheritance. What are the two components of the chromosome? __________________________________________________________________________ 2. From initial logic, which c ...
... 1. After Morgan and fellow scientists developed the Chromosomal Theory of Inheritance, the search was on for the chemical mechanism of inheritance. What are the two components of the chromosome? __________________________________________________________________________ 2. From initial logic, which c ...
AP 15-16 Test Review When Thomas Hunt Morgan crossed his red
... In cats, black fur color is caused by an X–linked allele; the other allele at this locus causes orange color. The heterozygote is tortoiseshell. What kinds of offspring would you expect from the cross of a black female and an orange male? Red-green color blindness is a sex-linked recessive trait in ...
... In cats, black fur color is caused by an X–linked allele; the other allele at this locus causes orange color. The heterozygote is tortoiseshell. What kinds of offspring would you expect from the cross of a black female and an orange male? Red-green color blindness is a sex-linked recessive trait in ...
L 17 _PCR
... dye terminators: instead of radioactive dNTPs, use ddNTPs with fluorescent tags, a different color in each dideoxy reaction. Then all four reactions can be run on a single lane, with the colors read by a laser as each band runs off the bottom of the gel. automated sequencers use cycle sequencing (li ...
... dye terminators: instead of radioactive dNTPs, use ddNTPs with fluorescent tags, a different color in each dideoxy reaction. Then all four reactions can be run on a single lane, with the colors read by a laser as each band runs off the bottom of the gel. automated sequencers use cycle sequencing (li ...
File
... ladder. The sides of the ladder consist of long chains made up of “P”s and “S”s. Each “P” stands for a ________________ and each “S” stands for a ____________. 5. The actual DNA structure is not really like a ladder, but like two coils wrapped around each other. This structure is called a double ___ ...
... ladder. The sides of the ladder consist of long chains made up of “P”s and “S”s. Each “P” stands for a ________________ and each “S” stands for a ____________. 5. The actual DNA structure is not really like a ladder, but like two coils wrapped around each other. This structure is called a double ___ ...
Dr. Wade Berrettini`s Powerpoint presentation
... ~1,000,000 SNP CHIPs provide the ability to obtain a genotype at 1 SNP every ~ 3000 base pairs in the genome, allowing determination of most common SNPs. Allele-specific fluorescently-tagged DNA fragments (known as oligonucleotides) are mounted on the slide. The oligonucleotides are sequence-specifi ...
... ~1,000,000 SNP CHIPs provide the ability to obtain a genotype at 1 SNP every ~ 3000 base pairs in the genome, allowing determination of most common SNPs. Allele-specific fluorescently-tagged DNA fragments (known as oligonucleotides) are mounted on the slide. The oligonucleotides are sequence-specifi ...
Genetic_Engineers_Mini
... Technique used for transferring DNA fragments from a gel to a membrane by capillary action prior to use for gene probing Having the ability to produce all cell types of the organism ...
... Technique used for transferring DNA fragments from a gel to a membrane by capillary action prior to use for gene probing Having the ability to produce all cell types of the organism ...
Chapter 13 Genetic Engineering
... • Gel Electrophoresis- DNA Fragments are placed in certain gel wells and an electric voltage is passed through them. • DNA molecules move toward the opposite end of the gel. • Smaller DNA fragments move faster through the gel. ...
... • Gel Electrophoresis- DNA Fragments are placed in certain gel wells and an electric voltage is passed through them. • DNA molecules move toward the opposite end of the gel. • Smaller DNA fragments move faster through the gel. ...
“Cowboy Glossary” of Genetic Terms
... Low Density Genomic Profile – a DNA test that uses 30,000 SNP markers; these 30K markers are then imputed up to 50K for GE-EPDs High Density Genomic Profile – a DNA test that uses 150,000 SNP markers, providing more genomic information; GE-EPDs are created by extracting 50K of these markers Genetic ...
... Low Density Genomic Profile – a DNA test that uses 30,000 SNP markers; these 30K markers are then imputed up to 50K for GE-EPDs High Density Genomic Profile – a DNA test that uses 150,000 SNP markers, providing more genomic information; GE-EPDs are created by extracting 50K of these markers Genetic ...
DNA extraction activity
... You will need Flash Player to run this simulation. Go to http://learn.genetics.utah.edu/content/labs/extraction/ Click on the “Start Lab” to begin. There are sound effects with this simulation, so if you’re in a lab, use headphones. 1. What are some reasons that scientists may need DNA samples? 2. T ...
... You will need Flash Player to run this simulation. Go to http://learn.genetics.utah.edu/content/labs/extraction/ Click on the “Start Lab” to begin. There are sound effects with this simulation, so if you’re in a lab, use headphones. 1. What are some reasons that scientists may need DNA samples? 2. T ...
Your name
... 19. What is the goal of the human genome project? Find a complete sequence of DNA for humans 20. List some organisms that genetic technology has “enhanced”. ...
... 19. What is the goal of the human genome project? Find a complete sequence of DNA for humans 20. List some organisms that genetic technology has “enhanced”. ...
Study guide
... using a made-up plasmid. We then created a restriction enzyme cut of the plasmid and inserted a synthetic DNA sequence. We followed that “transgenic” plasmid through transcription to mRNA, and then translation to an amino acid sequence. We briefly discussed what a clone is. We also discussed the use ...
... using a made-up plasmid. We then created a restriction enzyme cut of the plasmid and inserted a synthetic DNA sequence. We followed that “transgenic” plasmid through transcription to mRNA, and then translation to an amino acid sequence. We briefly discussed what a clone is. We also discussed the use ...
DNA Structure and Function
... that forms during translation. 4. What is the purpose of replication? P152 A. to make an RNA template from DNA B. to produce copies of a DNA molecule C. to move mRNA through the ribosome D. to change the number, type, or order of bases in DNA ...
... that forms during translation. 4. What is the purpose of replication? P152 A. to make an RNA template from DNA B. to produce copies of a DNA molecule C. to move mRNA through the ribosome D. to change the number, type, or order of bases in DNA ...
Name_____________________ Date__________ Class
... substituted with (or exchanged for) a different nucleotide that may result in an altered sequence of amino acid during translation. occurs when a DNA gene is damaged or changed in such a way as to alter the genetic message carried by that gene. is a type of mutation involving the loss of genetic mat ...
... substituted with (or exchanged for) a different nucleotide that may result in an altered sequence of amino acid during translation. occurs when a DNA gene is damaged or changed in such a way as to alter the genetic message carried by that gene. is a type of mutation involving the loss of genetic mat ...
DNA Sequencing
... yield bacteriophage, modified by adding: betagalactosidase screening, polylinker) Uses 32P-deoxynucleoside triphosphates ...
... yield bacteriophage, modified by adding: betagalactosidase screening, polylinker) Uses 32P-deoxynucleoside triphosphates ...
Supplementary Methods Sequencing of Multiplex PCR Amplicons
... Integrative Genomics Viewer; IGV v.2.1, Broad Institute). Droplet digital PCR To validate the mutations detected by deep sequencing, droplet digital PCR (ddPCR) for KRAS and GNAS was conducted with the Bio-Rad QX200 system using custom primer/probe set (see Supplementary Table 2). Genomic DNA (1-5 n ...
... Integrative Genomics Viewer; IGV v.2.1, Broad Institute). Droplet digital PCR To validate the mutations detected by deep sequencing, droplet digital PCR (ddPCR) for KRAS and GNAS was conducted with the Bio-Rad QX200 system using custom primer/probe set (see Supplementary Table 2). Genomic DNA (1-5 n ...
Document
... terminal sequence of the protein encoding this gene was known, however, and a synthetic oligonucleotide that corresponded to amino acids 1 through 10 of this protein was produced and labeled; it hybridized only to the 9 kb, 13 kb and 15 kb fragments. 6. True or false. The 3’ end of the mRNA made fro ...
... terminal sequence of the protein encoding this gene was known, however, and a synthetic oligonucleotide that corresponded to amino acids 1 through 10 of this protein was produced and labeled; it hybridized only to the 9 kb, 13 kb and 15 kb fragments. 6. True or false. The 3’ end of the mRNA made fro ...
SNP genotyping
SNP genotyping is the measurement of genetic variations of single nucleotide polymorphisms (SNPs) between members of a species. It is a form of genotyping, which is the measurement of more general genetic variation. SNPs are one of the most common types of genetic variation. An SNP is a single base pair mutation at a specific locus, usually consisting of two alleles (where the rare allele frequency is >1%). SNPs are found to be involved in the etiology of many human diseases and are becoming of particular interest in pharmacogenetics. Because SNPs are conserved during evolution, they have been proposed as markers for use in quantitative trait loci (QTL) analysis and in association studies in place of microsatellites. The use of SNPs is being extended in the HapMap project, which aims to provide the minimal set of SNPs needed to genotype the human genome. SNPs can also provide a genetic fingerprint for use in identity testing. The increase in interest in SNPs has been reflected by the furious development of a diverse range of SNP genotyping methods.