BIOB111 - Tutorial activity for Session 21
... Answer these questions a. Where in the cell does the citric acid cycle (Krebs cycle) occur b. ...
... Answer these questions a. Where in the cell does the citric acid cycle (Krebs cycle) occur b. ...
Glycolysis 2
... in the mitochondria to acetyl CoA, and ultimately to CO2 and H2O which are the products of the citrate cycle and electron transport chain. 2. Under anaerobic conditions, such as occurs in muscle cells during strenuous exercise, or in erythrocytes which lack mitochondria, pyruvate is converted to lac ...
... in the mitochondria to acetyl CoA, and ultimately to CO2 and H2O which are the products of the citrate cycle and electron transport chain. 2. Under anaerobic conditions, such as occurs in muscle cells during strenuous exercise, or in erythrocytes which lack mitochondria, pyruvate is converted to lac ...
ppt Oxygen Debt-Energy Systems - NCEA-Physical
... Difference between oxygen consumed during exercise and amount that would have been consumed had a steady rate, aerobic metabolism occurred at onset of exercise. ...
... Difference between oxygen consumed during exercise and amount that would have been consumed had a steady rate, aerobic metabolism occurred at onset of exercise. ...
View/Open - Oregon State University
... 5. In microorganisms, pyruvate is converted to ethanol during anaerobic conditions for the same reasons lactate is made in animals - because it creates NAD+ needed to keep glycolysis going when oxygen concentration is low. 6. Conversion of pyruvate to ethanol involves two enzymes - 1) pyruvate decar ...
... 5. In microorganisms, pyruvate is converted to ethanol during anaerobic conditions for the same reasons lactate is made in animals - because it creates NAD+ needed to keep glycolysis going when oxygen concentration is low. 6. Conversion of pyruvate to ethanol involves two enzymes - 1) pyruvate decar ...
生物化學小考(一) 範圍ch1~ch4
... (C) In yeast growing anaerobically, pyruvate is converted to ethanol. (D) Reduction of pyruvate to lactate regenerates a cofactor essential for glycolysis. (E)Under anaerobic conditions pyruvate does not form because glycolysis does not occur. 9. Which of the following compounds cannot serve as the ...
... (C) In yeast growing anaerobically, pyruvate is converted to ethanol. (D) Reduction of pyruvate to lactate regenerates a cofactor essential for glycolysis. (E)Under anaerobic conditions pyruvate does not form because glycolysis does not occur. 9. Which of the following compounds cannot serve as the ...
Determination of the DNA and Amino Acid Sequences of the Lactate
... Agarose gel electrophoresis was performed in a horizontal gel apparatus according to Sambrook et al. (6) following PCR. After the electrophoresis; 200 µl TE buffer (10 mM Tris HCl, 1 mM EDTA) was added to the gel slices containing the DNA, and they were incubated in a waterbath at 68ºC for 10 min. P ...
... Agarose gel electrophoresis was performed in a horizontal gel apparatus according to Sambrook et al. (6) following PCR. After the electrophoresis; 200 µl TE buffer (10 mM Tris HCl, 1 mM EDTA) was added to the gel slices containing the DNA, and they were incubated in a waterbath at 68ºC for 10 min. P ...
III. Metabolism
... The M-type predominates tissue that are subject to anaerobic conditions such as liver and skeletal muscle. H4 LDH has a low KM for pyruvate and is allosterically inhibited by it. M4 LDH has a low KM for pyruvate and is NOT allosterically inhibited by it. ...
... The M-type predominates tissue that are subject to anaerobic conditions such as liver and skeletal muscle. H4 LDH has a low KM for pyruvate and is allosterically inhibited by it. M4 LDH has a low KM for pyruvate and is NOT allosterically inhibited by it. ...
Glycolysis and Cellular Respiration
... Occurs under anaerobic conditions Pyruvate is converted into lactate or ethanol and CO2 Fermentation does not directly produce more ATP But is necessary to regenerate NAD+, which must be available for glycolysis to continue ...
... Occurs under anaerobic conditions Pyruvate is converted into lactate or ethanol and CO2 Fermentation does not directly produce more ATP But is necessary to regenerate NAD+, which must be available for glycolysis to continue ...
Concepts in Biochemistry 3/e
... Citric Acid Cycle, the Glyoxylate Cycle (Skip), and the Pentose Phosphate Pathway ...
... Citric Acid Cycle, the Glyoxylate Cycle (Skip), and the Pentose Phosphate Pathway ...
Short Answer Questions: a workshop
... Awareness of Strong and Weak Answers The next example is worth more (5 marks) and so requires a longer answer, re-reading the situation and allowing 5 minutes to write. Mark the 3 answers as if you were the tutor – what mark would each of them receive? What ...
... Awareness of Strong and Weak Answers The next example is worth more (5 marks) and so requires a longer answer, re-reading the situation and allowing 5 minutes to write. Mark the 3 answers as if you were the tutor – what mark would each of them receive? What ...
Summary of glycolysis (Embden
... during exercise, there is lack of oxygen so this reconversion is not possible, Therefore, the cell has to couple some other reaction in which NAD+ is regenerated in the cytoplasm itself hence, pyruvate is reduced to lactate; the NAD+ thus generated is reutilized for uninterrupted operation of the pa ...
... during exercise, there is lack of oxygen so this reconversion is not possible, Therefore, the cell has to couple some other reaction in which NAD+ is regenerated in the cytoplasm itself hence, pyruvate is reduced to lactate; the NAD+ thus generated is reutilized for uninterrupted operation of the pa ...
Ch 18 reading guide
... 7. Which cofactor is involved in redox and also forms a thioester? 8. In the process, FAD is reduced to _____________ as lipoamide is reoxidized. In turn, _______________ is reoxidized to FAD as NAD+ is reduced to ________________. 9. Which cofactor also serves as a flexible linkage to bring the sub ...
... 7. Which cofactor is involved in redox and also forms a thioester? 8. In the process, FAD is reduced to _____________ as lipoamide is reoxidized. In turn, _______________ is reoxidized to FAD as NAD+ is reduced to ________________. 9. Which cofactor also serves as a flexible linkage to bring the sub ...
Total tissue lactate dehydrogenase activity in endometrial carcinoma
... Abstract. Šimaga Š, Abramic M, Osmak M, Babic D, Ilic-Forko J. Total tissue lactate dehydrogenase activity in endometrial carcinoma. Int J Gynecol Cancer 2008. Lactate dehydrogenase (LDH) is essential for continuous glycolysis necessary for accelerated tumor growth. The aim of this study was to ...
... Abstract. Šimaga Š, Abramic M, Osmak M, Babic D, Ilic-Forko J. Total tissue lactate dehydrogenase activity in endometrial carcinoma. Int J Gynecol Cancer 2008. Lactate dehydrogenase (LDH) is essential for continuous glycolysis necessary for accelerated tumor growth. The aim of this study was to ...
VO2 Max
... GENETICS: heredity can account for up to 25-50% of the variance seen between individuals. (e.g. skeletal muscle composition, structure of cardio respiratory). There is a genetic upper limit beyond which, further increases in either intensity or volume have no effect on aerobic power AGE: in gene ...
... GENETICS: heredity can account for up to 25-50% of the variance seen between individuals. (e.g. skeletal muscle composition, structure of cardio respiratory). There is a genetic upper limit beyond which, further increases in either intensity or volume have no effect on aerobic power AGE: in gene ...
Dear Notetaker:
... a. Lipocalin 17. The effects of a competitive inhibitor on the enzyme kinetics will be to: a. Decrease the apparent affinity of the substrate for the enzyme. i. (Review competitive vs non competitive ) 18. The predominant corneal lactate dehydrogenase (LDH) is H1M3, which can do two (2) of the follo ...
... a. Lipocalin 17. The effects of a competitive inhibitor on the enzyme kinetics will be to: a. Decrease the apparent affinity of the substrate for the enzyme. i. (Review competitive vs non competitive ) 18. The predominant corneal lactate dehydrogenase (LDH) is H1M3, which can do two (2) of the follo ...
ATP - Mhanafi123`s Blog
... with 7 ATP as high energy phosphate. ( older textbook still counting as 8 ATP). In unaerobic Glycolysis of certain type of muscle for sprinters, lack of oxygen cause inactive respiratory chain. NADH will reduces Pyruvate, and Lactate is the final product of Glycolysis. NAD+ is ready as coenzyme for ...
... with 7 ATP as high energy phosphate. ( older textbook still counting as 8 ATP). In unaerobic Glycolysis of certain type of muscle for sprinters, lack of oxygen cause inactive respiratory chain. NADH will reduces Pyruvate, and Lactate is the final product of Glycolysis. NAD+ is ready as coenzyme for ...
This is Most of an Old Exam
... Cellular oxidation of food fuels is the immediate source of electrons for oxidative phosphorylation. B. In oxidative phosphorylation, both the electron transport proteins and the ATP synthase molecules are in the same membrane. C. NAD+ and FAD+ are hydrogen carrier molecules. NAD+ can carry one hydr ...
... Cellular oxidation of food fuels is the immediate source of electrons for oxidative phosphorylation. B. In oxidative phosphorylation, both the electron transport proteins and the ATP synthase molecules are in the same membrane. C. NAD+ and FAD+ are hydrogen carrier molecules. NAD+ can carry one hydr ...
Cellular Respiration Scenarios – Teacher Answers
... needed to get rid of all the lactate. C) Discuss how the body rids itself of lactic acid following anaerobic respiration. How does it use the lactic acid for a good purpose? As mentioned in B, lactate is converted back to pyruvate when oxygen becomes available again. Thus, the energy potential from ...
... needed to get rid of all the lactate. C) Discuss how the body rids itself of lactic acid following anaerobic respiration. How does it use the lactic acid for a good purpose? As mentioned in B, lactate is converted back to pyruvate when oxygen becomes available again. Thus, the energy potential from ...
Once this was accomplished we were able to study
... with the wet canning technique than with the dry canning technique, wet canning was employed in all the LDH assays. Lowry and associates have shown that the loss of activity in the dry canning technique was due to condensation of water on the small fragment of tissue before the incubation medium was ...
... with the wet canning technique than with the dry canning technique, wet canning was employed in all the LDH assays. Lowry and associates have shown that the loss of activity in the dry canning technique was due to condensation of water on the small fragment of tissue before the incubation medium was ...
Ethylene Glycol Intoxication
... - ethylene glycol level - severe metabolic acidosis (high anion gap) from glycolic acid accumulation - very high lactate (artefactual as there is high cross reactivity between lactate and glycolate in laboratory analysis) -> high lactate with oxidase method, less high with lactate dehydrogenase meth ...
... - ethylene glycol level - severe metabolic acidosis (high anion gap) from glycolic acid accumulation - very high lactate (artefactual as there is high cross reactivity between lactate and glycolate in laboratory analysis) -> high lactate with oxidase method, less high with lactate dehydrogenase meth ...
Gluconeogenesis Precursors for Gluconeogenesis
... • A liver biopsy of a four‐year old boy indicated that the F‐1,6‐Bpase enzyme activity was 20% normal. The patient’s blood glucose levels were normal at the beginning of a fast, but then decreased suddenly. Pyruvate and alanine concentrations were also elevated, as was the glyceraldehyde/DHAP ...
... • A liver biopsy of a four‐year old boy indicated that the F‐1,6‐Bpase enzyme activity was 20% normal. The patient’s blood glucose levels were normal at the beginning of a fast, but then decreased suddenly. Pyruvate and alanine concentrations were also elevated, as was the glyceraldehyde/DHAP ...
HEMOGLOBIN
... due to: (a) incompatible blood transfusion (b) hemolytic anemia 2) HEPATIC JAUNDICE: can be due to: A) Decreased glucouronyl transferase as in physiologic jaundice of newborn. There is increase in unconjugated lipid soluble bilirubin which can cross the blood brain barrier and produce encephalopathy ...
... due to: (a) incompatible blood transfusion (b) hemolytic anemia 2) HEPATIC JAUNDICE: can be due to: A) Decreased glucouronyl transferase as in physiologic jaundice of newborn. There is increase in unconjugated lipid soluble bilirubin which can cross the blood brain barrier and produce encephalopathy ...
05. Clinical enzymology (1)
... A major disadvantage in the use of enzymes for the diagnosis of tissue damage is their lack of specificity to a particular tissue or cell type. Many enzymes are common to more than one tissue. ...
... A major disadvantage in the use of enzymes for the diagnosis of tissue damage is their lack of specificity to a particular tissue or cell type. Many enzymes are common to more than one tissue. ...
Lactate dehydrogenase
A lactate dehydrogenase (LDH or LD) is an enzyme found in nearly all living cells (animals, plants, and prokaryotes). LDH catalyzes the conversion of pyruvate to lactate and back, as it converts NADH to NAD+ and back. A dehydrogenase is an enzyme that transfers a hydride from one molecule to another.LDH exist in four distinct enzyme classes. This article is about the common NAD(P)-dependent L-lactate dehydrogenase. Other LDHs act on D-lactate and/or are dependent on cytochrome c: D-lactate dehydrogenase (cytochrome)) and L-lactate (L-lactate dehydrogenase (cytochrome)). LDH has been of medical significance because it is found extensively in body tissues, such as blood cells and heart muscle. Because it is released during tissue damage, it is a marker of common injuries and disease such as heart failure.