Application of Bruchin B to pea pods results in
... confirmed using liquid chromatography-mass spectrometry (LCMS), and a set of spiked samples allowed an estimate of recovery (87.564.9%, mean 6standard error of the mean for n=5), which was used in the estimates of pisatin level. A sample of authentic pisatin (recrystallized from heptane) was prepare ...
... confirmed using liquid chromatography-mass spectrometry (LCMS), and a set of spiked samples allowed an estimate of recovery (87.564.9%, mean 6standard error of the mean for n=5), which was used in the estimates of pisatin level. A sample of authentic pisatin (recrystallized from heptane) was prepare ...
Efficient expression of ribozyme and reduction of stromelysin mRNA
... treatment. The attractive features of AAV as a vector are nonpathogenicity, low immunogenicity, stable and efficient expression of transgenes from the integrated or episomal form, infection of non-dividing cells, broad host range, generation of high titer (8 x 108 IU/ml), and physically stable virio ...
... treatment. The attractive features of AAV as a vector are nonpathogenicity, low immunogenicity, stable and efficient expression of transgenes from the integrated or episomal form, infection of non-dividing cells, broad host range, generation of high titer (8 x 108 IU/ml), and physically stable virio ...
An Arabidopsis Mutant with a Reduced Leve1 of cabl40 RNA 1s a
... overexpress the gene in a host plant and observe the resulting phenotype. This approach has been successful in many cases (Boylan and Quail, 1989; Kay et al., 1989; Keller et al., 1989; Martineau et al., 1989; Tepperman and Dunsmuir, 1990; Wagner et al., 1991), but there have been a number of instan ...
... overexpress the gene in a host plant and observe the resulting phenotype. This approach has been successful in many cases (Boylan and Quail, 1989; Kay et al., 1989; Keller et al., 1989; Martineau et al., 1989; Tepperman and Dunsmuir, 1990; Wagner et al., 1991), but there have been a number of instan ...
Chapter 2. Structures of Nucleic Acids
... mice (Fig. 2.1.A.). Smooth (S) strains produce a capsular polysaccharide on their surface, which allow the Pneumococi to escape destruction by the mouse, and the infection proceeds, i.e. they are virulent. This polysaccaride can be type I, II, or III. Virulent S strains can be killed by heat (i.e., ...
... mice (Fig. 2.1.A.). Smooth (S) strains produce a capsular polysaccharide on their surface, which allow the Pneumococi to escape destruction by the mouse, and the infection proceeds, i.e. they are virulent. This polysaccaride can be type I, II, or III. Virulent S strains can be killed by heat (i.e., ...
Biology
... Using clues from Franklin’s pattern, James Watson and Francis Crick built a model that explained how DNA carried information and could be copied. Watson and Crick's model of DNA was a double helix, in which two strands were wound around each other. ...
... Using clues from Franklin’s pattern, James Watson and Francis Crick built a model that explained how DNA carried information and could be copied. Watson and Crick's model of DNA was a double helix, in which two strands were wound around each other. ...
a heat-sensitive cellular function located in the nucleolus
... bacterial and viral development (2-4, 10, 12). In a first attempt at a synthesis based on extensive work on poliovirus, Lwoff (13) established a critical thermosensitive event involving hydrogen bonds in the viral cycle and advanced the working hypothesis that this event is the polymerization of a m ...
... bacterial and viral development (2-4, 10, 12). In a first attempt at a synthesis based on extensive work on poliovirus, Lwoff (13) established a critical thermosensitive event involving hydrogen bonds in the viral cycle and advanced the working hypothesis that this event is the polymerization of a m ...
The WD40-Repeat Proteins NFC101 and NFC102
... complexes. In this study, we generated transgenic lines with simultaneous nfc101 and nfc102 downregulation and analyzed phenotypic alterations, along with effects on RNA levels, the binding of NFC101/NFC102, and Rpd3-type histone deacetylases (HDACs), and histone modifications at selected targets. Di ...
... complexes. In this study, we generated transgenic lines with simultaneous nfc101 and nfc102 downregulation and analyzed phenotypic alterations, along with effects on RNA levels, the binding of NFC101/NFC102, and Rpd3-type histone deacetylases (HDACs), and histone modifications at selected targets. Di ...
Role of base-backbone and base-base interactions
... or overtwists, so that the methyl groups move away from the backbones, both lead the step towards the A-conformation. TA is most flexible as it does not have such restriction. These characteristics are observed with other pyrimidine-pyrimidine, pyrimidine-purine, purine-pyrimidine steps, respectivel ...
... or overtwists, so that the methyl groups move away from the backbones, both lead the step towards the A-conformation. TA is most flexible as it does not have such restriction. These characteristics are observed with other pyrimidine-pyrimidine, pyrimidine-purine, purine-pyrimidine steps, respectivel ...
Analysis of aptamer sequence activity relationshipsw
... during the design of drug candidates.12 SELEX has been used in the characterization of transcription factor binding sites, by the selection and enrichment of DNA sequences with high affinity to the target protein over several iterations. Good binders are sequenced and used to construct a weight matrix ...
... during the design of drug candidates.12 SELEX has been used in the characterization of transcription factor binding sites, by the selection and enrichment of DNA sequences with high affinity to the target protein over several iterations. Good binders are sequenced and used to construct a weight matrix ...
Energetics of protein–DNA interactions
... of protein–DNA interactions provide additional motivation for investigation. These unique features are most apparent when one compares the study of protein–DNA interactions to two other fields where molecular interactions play an important role: protein folding (self-interactions along a polymer chai ...
... of protein–DNA interactions provide additional motivation for investigation. These unique features are most apparent when one compares the study of protein–DNA interactions to two other fields where molecular interactions play an important role: protein folding (self-interactions along a polymer chai ...
Chpt2_Struc_Nucleic_Acids.doc
... mice (Fig. 2.1.A.). Smooth (S) strains produce a capsular polysaccharide on their surface, which allow the Pneumococi to escape destruction by the mouse, and the infection proceeds, i.e. they are virulent. This polysaccaride can be type I, II, or III. Virulent S strains can be killed by heat (i.e., ...
... mice (Fig. 2.1.A.). Smooth (S) strains produce a capsular polysaccharide on their surface, which allow the Pneumococi to escape destruction by the mouse, and the infection proceeds, i.e. they are virulent. This polysaccaride can be type I, II, or III. Virulent S strains can be killed by heat (i.e., ...
Document
... All downloads and use of the Osprey Network Visualization System are subject to the following terms: As long as you comply with the terms of this Agreement, you are granted a non-exclusive license to install and use the Software on a single computer for educational, research, and not-forprofit purpo ...
... All downloads and use of the Osprey Network Visualization System are subject to the following terms: As long as you comply with the terms of this Agreement, you are granted a non-exclusive license to install and use the Software on a single computer for educational, research, and not-forprofit purpo ...
Molecular Biology of Transcription and RNA Processing
... The central dogma of biology describes the flow of genetic information from DNA to RNA to protein (see Figure 1.8). It conveys that DNA is the repository of genetic information, which is converted through transcription into RNA, one type of which is then translated into protein. Transcription is the ...
... The central dogma of biology describes the flow of genetic information from DNA to RNA to protein (see Figure 1.8). It conveys that DNA is the repository of genetic information, which is converted through transcription into RNA, one type of which is then translated into protein. Transcription is the ...
Monoallelic Expression and Dominance
... transcripts were reduced by only approximately 10% in heterozygotes relative to Sb homozygotes. Importantly, comparison of eight SaSa and eight SaSb plants generated by forced selfing of an SaSb plant in which SCRa was “silent” showed that SCRa was expressed in SaSa progeny and “silenced” in their S ...
... transcripts were reduced by only approximately 10% in heterozygotes relative to Sb homozygotes. Importantly, comparison of eight SaSa and eight SaSb plants generated by forced selfing of an SaSb plant in which SCRa was “silent” showed that SCRa was expressed in SaSa progeny and “silenced” in their S ...
A new heavy lanthanide-dependent DNAzyme
... among the six. They all have a stretch of pyrimidines followed by a few purines in the loop (this is also true for Tm8, see Supplementary Figure S2). The other three DNAzymes are slower and their base contents in the loop region are more mixed up, especially on the pyrimidine side. It is also intere ...
... among the six. They all have a stretch of pyrimidines followed by a few purines in the loop (this is also true for Tm8, see Supplementary Figure S2). The other three DNAzymes are slower and their base contents in the loop region are more mixed up, especially on the pyrimidine side. It is also intere ...
Interfering RNA
... RNAi Patentability Issues 35 U.S.C. 112, first paragraph, Written Description • The Analysis: – identify all disclosed relevant distinguishing characteristics as they relate to the scope and content of the claims – identify any disclosed structure/function relationships • what target regions are ac ...
... RNAi Patentability Issues 35 U.S.C. 112, first paragraph, Written Description • The Analysis: – identify all disclosed relevant distinguishing characteristics as they relate to the scope and content of the claims – identify any disclosed structure/function relationships • what target regions are ac ...
Evidence for Variable Selective Pressures at a
... et al. 2006). In this context, a DNA secondary structure refers to the molecular-folded structure formed when a singlestranded DNA molecule folds back upon itself. Typically, unpaired and mispaired bases in a secondary structure form single-stranded loops that are more vulnerable to mutations than p ...
... et al. 2006). In this context, a DNA secondary structure refers to the molecular-folded structure formed when a singlestranded DNA molecule folds back upon itself. Typically, unpaired and mispaired bases in a secondary structure form single-stranded loops that are more vulnerable to mutations than p ...
Full report
... These plots are drawn for all protein, RNA and DNA chains in the entry. The first graphic for a chain summarises the proportions of the various outlier classes displayed in the second graphic. The second graphic shows the sequence view annotated by issues in geometry and electron density. Residues a ...
... These plots are drawn for all protein, RNA and DNA chains in the entry. The first graphic for a chain summarises the proportions of the various outlier classes displayed in the second graphic. The second graphic shows the sequence view annotated by issues in geometry and electron density. Residues a ...
Distinct Roles for Drosophila Dicer-1 and Dicer
... dsRNA processing depletion of dicer results in reduced effectiveness of injected siRNA Dicer binds to components of RISC (R2D2) & binds tightly to siRNA Role of Dicer in siRISC is not well characterized… The authors took a genetic approach to study Dicer function in Drosophila ...
... dsRNA processing depletion of dicer results in reduced effectiveness of injected siRNA Dicer binds to components of RISC (R2D2) & binds tightly to siRNA Role of Dicer in siRISC is not well characterized… The authors took a genetic approach to study Dicer function in Drosophila ...
NIH Public Access
... providing a powerful model to investigate long-range chromosomal regulation. This review focuses on events downstream of the spread of XIST RNA across the interphase chromosome, to consider how this large non-coding RNA interacts with and silences a whole chromosome. Several lines of evidence are su ...
... providing a powerful model to investigate long-range chromosomal regulation. This review focuses on events downstream of the spread of XIST RNA across the interphase chromosome, to consider how this large non-coding RNA interacts with and silences a whole chromosome. Several lines of evidence are su ...
Lecture 25: Protein Synthesis
... Explain what a polysome is. Understand how ribosomes place themselves on the start codon. This is different in bacteria and in eukaryotes. Be able to compare and contrast these mechanisms. Understand what bacterial IF-1 and IF-3 do. Understand what the eukaryotic eIF4 complex does. Understand what a ...
... Explain what a polysome is. Understand how ribosomes place themselves on the start codon. This is different in bacteria and in eukaryotes. Be able to compare and contrast these mechanisms. Understand what bacterial IF-1 and IF-3 do. Understand what the eukaryotic eIF4 complex does. Understand what a ...
! Mendel`s Law of Independent Assortment
... see that each of these is ¼ of the total number of squares. How do we get the phenotypic results? The sum rule of probability tells us that when the same event can occur in more than one way, we can add the results. Because 1, 2, and 3 all result in unattached earlobes, we add them up to know that t ...
... see that each of these is ¼ of the total number of squares. How do we get the phenotypic results? The sum rule of probability tells us that when the same event can occur in more than one way, we can add the results. Because 1, 2, and 3 all result in unattached earlobes, we add them up to know that t ...
Crystal structure of the nucleosome core particle at 2.8 Å
... between the gyros of the DNA superhelix to contact neighboring particles. The lack of uniformity between multiple histone/DNA-binding sites causes the DNA to deviate from ideal superhelix geometry. DNA in chromatin is organized in arrays of nucleosomes (1). Two copies of each histone protein, H2A, H ...
... between the gyros of the DNA superhelix to contact neighboring particles. The lack of uniformity between multiple histone/DNA-binding sites causes the DNA to deviate from ideal superhelix geometry. DNA in chromatin is organized in arrays of nucleosomes (1). Two copies of each histone protein, H2A, H ...
Chapter 24 Translation
... • The mechanism is similar in bacteria (which have two types of class 1 release factors) and eukaryotes (which have only one class 1 release factor). ...
... • The mechanism is similar in bacteria (which have two types of class 1 release factors) and eukaryotes (which have only one class 1 release factor). ...
10 Day Lesson Plan - Joseph L. Anderson
... we examine the process further, we must know the fundamental difference between DNA and RNA. RNA does not have two sides as DNA. There is a slight difference in the sugar phosphate structure. RNA has one base that is different than DNA. This base replaces thymine and is called uracil. RNA also comes ...
... we examine the process further, we must know the fundamental difference between DNA and RNA. RNA does not have two sides as DNA. There is a slight difference in the sugar phosphate structure. RNA has one base that is different than DNA. This base replaces thymine and is called uracil. RNA also comes ...
Nucleic acid tertiary structure
The tertiary structure of a nucleic acid is its precise three-dimensional structure, as defined by the atomic coordinates. RNA and DNA molecules are capable of diverse functions ranging from molecular recognition to catalysis. Such functions require a precise three-dimensional tertiary structure. While such structures are diverse and seemingly complex, they are composed of recurring, easily recognizable tertiary structure motifs that serve as molecular building blocks. Some of the most common motifs for RNA and DNA tertiary structure are described below, but this information is based on a limited number of solved structures. Many more tertiary structural motifs will be revealed as new RNA and DNA molecules are structurally characterized.