The trp Operon - aandersonbiology
... and automatically used in every cell all the time. Each cell uses or expresses only a small portion of its total number of genes. The finger cells are not expressing the gene for insulin production. In 1961, Fracois Jacob and Jacques Monod described the control of gene expression as the operon model ...
... and automatically used in every cell all the time. Each cell uses or expresses only a small portion of its total number of genes. The finger cells are not expressing the gene for insulin production. In 1961, Fracois Jacob and Jacques Monod described the control of gene expression as the operon model ...
IMPROVE SMALL RNA-MEDIATED GENE SILENCING
... The biogenesis of heterochromatic siRNA requires specific members of the RDR, DCL, and AGO gene families. In the current model, plant-specific PolIV generates an aberrant RNA from methylated DNA templates (Figure 1.2) (Mosher et al. 2008). This aberrant RNA is then converted into dsRNA by RDR2 and ...
... The biogenesis of heterochromatic siRNA requires specific members of the RDR, DCL, and AGO gene families. In the current model, plant-specific PolIV generates an aberrant RNA from methylated DNA templates (Figure 1.2) (Mosher et al. 2008). This aberrant RNA is then converted into dsRNA by RDR2 and ...
PDF
... median length 159 bp (mean length 357 bp), whereas non-first introns had median length 118 bp (mean 210 bp). It should be noted that only first intron pairs in which both introns were first introns were considered, and the same criterion was used for non-first introns. First introns are significantl ...
... median length 159 bp (mean length 357 bp), whereas non-first introns had median length 118 bp (mean 210 bp). It should be noted that only first intron pairs in which both introns were first introns were considered, and the same criterion was used for non-first introns. First introns are significantl ...
p68/DDX5 DEAD-box RNA helicase gene encodes a novel miRNA
... that while p68 RNA helicase activity appears to be important for some functions (e.g., RNA processing), it does not appear to be required for its role as a transcriptional coactivator. The p68 gene contains a large intron (intron 11, 1.2 kb in the human gene), which has been conserved through evolut ...
... that while p68 RNA helicase activity appears to be important for some functions (e.g., RNA processing), it does not appear to be required for its role as a transcriptional coactivator. The p68 gene contains a large intron (intron 11, 1.2 kb in the human gene), which has been conserved through evolut ...
Kallikrein-like prorenin-converting enzymes in inbred
... at the molecular level is not clear at all and requires further investigation. We are currently analyzing the promoter region of PRCE C (mK9) of BPH and BPN strains of mice for its sequence and DNA binding proteins. The increased expression of PRCE C (mK9) in BPH mice raises the possibility of its i ...
... at the molecular level is not clear at all and requires further investigation. We are currently analyzing the promoter region of PRCE C (mK9) of BPH and BPN strains of mice for its sequence and DNA binding proteins. The increased expression of PRCE C (mK9) in BPH mice raises the possibility of its i ...
Table of Contents - Scholars` Bank
... allowed to “wobble” in its pairing. This means that between guanine and uracil there is a bond that is looser than those of traditional Watson-Crick base pairing. However, the nucleotide at position 34, even if it is a guanine or a uracil, can still form a base pair following standard Watson-Crick b ...
... allowed to “wobble” in its pairing. This means that between guanine and uracil there is a bond that is looser than those of traditional Watson-Crick base pairing. However, the nucleotide at position 34, even if it is a guanine or a uracil, can still form a base pair following standard Watson-Crick b ...
Localization and structural analysis of the ribosomal RNA operons of
... E. coli rRNA operons. The fact that insertion of a cartridge resulted in the loss of the wild type signal, leads to the conclusion that there are only three rRNA operons in R. sphaeroides. Thus rmA can be assigned to the 10 kb BamYU signal, rrnB to the 14 kb BamYU signal, and rmC to the 13 kb BamYU ...
... E. coli rRNA operons. The fact that insertion of a cartridge resulted in the loss of the wild type signal, leads to the conclusion that there are only three rRNA operons in R. sphaeroides. Thus rmA can be assigned to the 10 kb BamYU signal, rrnB to the 14 kb BamYU signal, and rmC to the 13 kb BamYU ...
A global picture of tRNA genes in plant genomes
... monocots, respectively. The internal or external motifs required for efficient transcription of tRNA genes by RNA polymerase III are well conserved among angiosperms. A brief analysis of the mitochondrial and plastidial tRNA gene populations is also provided. Keywords: alga, angiosperm, non-coding R ...
... monocots, respectively. The internal or external motifs required for efficient transcription of tRNA genes by RNA polymerase III are well conserved among angiosperms. A brief analysis of the mitochondrial and plastidial tRNA gene populations is also provided. Keywords: alga, angiosperm, non-coding R ...
ABSTRACT Title of Document: PROGRAMMED
... the A site. These bases include G529, G530, A1492, and A1493, which are all required for viability and tRNA binding (Ogle et al, 2005). When a cognate tRNA is in the A site, A1492 and A1493 are displaced into the minor groove of helix 44, and G530 switches from a syn to anti conformation. The first ...
... the A site. These bases include G529, G530, A1492, and A1493, which are all required for viability and tRNA binding (Ogle et al, 2005). When a cognate tRNA is in the A site, A1492 and A1493 are displaced into the minor groove of helix 44, and G530 switches from a syn to anti conformation. The first ...
Price, DK, Zhang, F, Ashley, CT and Warren, ST: The chicken FMR1 gene is highly conserved containing a CTT 5\' untranslated repeat and encodes an RNA-binding protein. Genomics 31:3-12 (1996).
... frequent inherited form of mental retardation. Fragile X syndrome segregates as an X-linked dominant disorder with reduced penetrance and has been mapped to the fragile X mental retardation (FMR1) gene located at human Xq27.3 (Warren and Ashley, 1995; Warren and Nelson, 1994). FMR1 contains a (CGG)n ...
... frequent inherited form of mental retardation. Fragile X syndrome segregates as an X-linked dominant disorder with reduced penetrance and has been mapped to the fragile X mental retardation (FMR1) gene located at human Xq27.3 (Warren and Ashley, 1995; Warren and Nelson, 1994). FMR1 contains a (CGG)n ...
informe tecnológico de patentes
... disorder caused by the expression of mutant huntingtin protein (Htt). Suppression of Htt expression, using RNA interference, might be an effective therapy. However, if reduction of wild-type protein is not well tolerated in the brain, it may be necessary to suppress just the product of the mutant al ...
... disorder caused by the expression of mutant huntingtin protein (Htt). Suppression of Htt expression, using RNA interference, might be an effective therapy. However, if reduction of wild-type protein is not well tolerated in the brain, it may be necessary to suppress just the product of the mutant al ...
Endonucleolytic processing of CCAless tRNA precursors by RNase
... In the archaea, the maturation of the 3¢ end of tRNAs occurs by endonucleolytic cleavage 3¢ to the discriminator base (Schierling et al., 2002), the unpaired base immediately preceding the CCA motif and used as an identity element by many aminoacyl-tRNA synthetases. In yeast and higher eukaryota, tw ...
... In the archaea, the maturation of the 3¢ end of tRNAs occurs by endonucleolytic cleavage 3¢ to the discriminator base (Schierling et al., 2002), the unpaired base immediately preceding the CCA motif and used as an identity element by many aminoacyl-tRNA synthetases. In yeast and higher eukaryota, tw ...
paper - Université de Namur
... understand the X-ray images of DNA. However the use of the CCV theory for its originally intended purpose [3], namely for the interpretation of X-ray diffraction by protein α-helices, is a bit too advanced and will not be pursued in the present didactic paper (a recent review [4] covers this and fur ...
... understand the X-ray images of DNA. However the use of the CCV theory for its originally intended purpose [3], namely for the interpretation of X-ray diffraction by protein α-helices, is a bit too advanced and will not be pursued in the present didactic paper (a recent review [4] covers this and fur ...
An Introduction to RNA Interference (RNAi)
... RNA molecules into the nematode worm C. elegans, Fire and Mello observed that it was double-stranded RNAs (dsRNAs) that were the actual inducers of the RNAi gene silencing phenomenon. dsRNAs are similar in structure to the double-stranded DNA helix that makes up our genome, only that DNA is replaced ...
... RNA molecules into the nematode worm C. elegans, Fire and Mello observed that it was double-stranded RNAs (dsRNAs) that were the actual inducers of the RNAi gene silencing phenomenon. dsRNAs are similar in structure to the double-stranded DNA helix that makes up our genome, only that DNA is replaced ...
A rule-based kinetic model of RNA polymerase II C
... modelled as acting on Ser2P during elongation and on both Ser2P and Ser5P during recycling. The Ser2 kinase Bur1 is recruited to CTD repeats of elongating RNAPII phosphorylated on Ser5. Bur1 augments the major Ser2 kinase (Ctk1) and is capable of phosphorylating CTD repeats at Ser2, when Ctk1 is ...
... modelled as acting on Ser2P during elongation and on both Ser2P and Ser5P during recycling. The Ser2 kinase Bur1 is recruited to CTD repeats of elongating RNAPII phosphorylated on Ser5. Bur1 augments the major Ser2 kinase (Ctk1) and is capable of phosphorylating CTD repeats at Ser2, when Ctk1 is ...
Novel mutants of 23S RNA: characterization of
... double mutant ribosomes U1067/C2505 and U1067/C2583 was in both strains dependent on Mg 2+ concentration: at 25 mM the resistance was nearly 2 times higher than at 10 mM (Table I)It is clear from table I that in all cases the resistance to thiostrepton was higher on the ribosomes derived from E.coli ...
... double mutant ribosomes U1067/C2505 and U1067/C2583 was in both strains dependent on Mg 2+ concentration: at 25 mM the resistance was nearly 2 times higher than at 10 mM (Table I)It is clear from table I that in all cases the resistance to thiostrepton was higher on the ribosomes derived from E.coli ...
Echinomycin binding to alternating AT
... modification of CCCG, CCGC and CGGC. For CCCG the CpG step will be occupied by the ligand under all conditions and result in strong reaction at the first adenine (CGA) on the 3'-side. The neighbour exclusion principle will forbid drug binding at the first ApT, but the second ApT is in the correct po ...
... modification of CCCG, CCGC and CGGC. For CCCG the CpG step will be occupied by the ligand under all conditions and result in strong reaction at the first adenine (CGA) on the 3'-side. The neighbour exclusion principle will forbid drug binding at the first ApT, but the second ApT is in the correct po ...
Prediction of biometallic interactions: challenges and applications Ph.D.Thesis Elisabeth Ortega Carrasco
... Transition metal ions are fundamental in Nature. Despite the general opinion, their presence in biological processes is not a man-made fact but a process that prompted early during the evolution. Transition metals have provided life with efficient catalysts and other optimal processes that would not ...
... Transition metal ions are fundamental in Nature. Despite the general opinion, their presence in biological processes is not a man-made fact but a process that prompted early during the evolution. Transition metals have provided life with efficient catalysts and other optimal processes that would not ...
Identity elements in tRNA-mediated transcription
... Fig. 3. Effect of native and chimeric tRNAs on transcription antitermination in the trpE-lacZ fusion. RNA was isolated from L. lactis cells after a growth medium shift as indicated in Methods and used to prepare a Northern blot that was hybridized with a lacZ-specific probe to visualize full length ...
... Fig. 3. Effect of native and chimeric tRNAs on transcription antitermination in the trpE-lacZ fusion. RNA was isolated from L. lactis cells after a growth medium shift as indicated in Methods and used to prepare a Northern blot that was hybridized with a lacZ-specific probe to visualize full length ...
Molecular mechanics of the interactions of spermine with DNA: DNA
... Present address: Code 6030, Laboratory for the Structure of Matter, Naval Research Laboratory, Washington DC 20375-5000, USA ...
... Present address: Code 6030, Laboratory for the Structure of Matter, Naval Research Laboratory, Washington DC 20375-5000, USA ...
Topologically Non-linked Circular Duplex DNA
... When one considers the question carefully, one’s thoughts are compelled in particular directions by the body of data which exists concerning the behavior of circular DNA in various settings. We find that we must make certain assumptions. Each of these assumptions can be readily challenged, and we ca ...
... When one considers the question carefully, one’s thoughts are compelled in particular directions by the body of data which exists concerning the behavior of circular DNA in various settings. We find that we must make certain assumptions. Each of these assumptions can be readily challenged, and we ca ...
In vivo evidence for the prokaryotic model of extended codon
... (lane 1), a well-de®ned set of proteins is labeled in WT cells, including cyt f, as evidenced by the speci®c absence of this species in strain DpetA, in which the petA gene is deleted (lane 2). Apart from these controls, the results showed that the ectopic expression of either the WT-A37, U37 or G37 ...
... (lane 1), a well-de®ned set of proteins is labeled in WT cells, including cyt f, as evidenced by the speci®c absence of this species in strain DpetA, in which the petA gene is deleted (lane 2). Apart from these controls, the results showed that the ectopic expression of either the WT-A37, U37 or G37 ...
Protocol for Real-Time RT-PCR - MGH-PGA
... to the appropriate templates, we obtained positive PCR results in contrast to the otherwise negative PCRs (see our paper for more details). Poor PCR amplification efficiency. The accuracy of real-time PCR is highly dependent on PCR efficiency. A reasonable efficiency should be at least 80%. Poor pri ...
... to the appropriate templates, we obtained positive PCR results in contrast to the otherwise negative PCRs (see our paper for more details). Poor PCR amplification efficiency. The accuracy of real-time PCR is highly dependent on PCR efficiency. A reasonable efficiency should be at least 80%. Poor pri ...
Discovering the Distribution of Palindromic Sequences in the
... however many base pairs were found. The results only account for perfect palindromic sequences, not approximate palindromes. It is important to find DNA palindromes in RNA because of tendencies of forming hairpin loops and pseudoknots which are key components of secondary, tertiary, and quaternary s ...
... however many base pairs were found. The results only account for perfect palindromic sequences, not approximate palindromes. It is important to find DNA palindromes in RNA because of tendencies of forming hairpin loops and pseudoknots which are key components of secondary, tertiary, and quaternary s ...
Nucleic acid tertiary structure
The tertiary structure of a nucleic acid is its precise three-dimensional structure, as defined by the atomic coordinates. RNA and DNA molecules are capable of diverse functions ranging from molecular recognition to catalysis. Such functions require a precise three-dimensional tertiary structure. While such structures are diverse and seemingly complex, they are composed of recurring, easily recognizable tertiary structure motifs that serve as molecular building blocks. Some of the most common motifs for RNA and DNA tertiary structure are described below, but this information is based on a limited number of solved structures. Many more tertiary structural motifs will be revealed as new RNA and DNA molecules are structurally characterized.