Lecture PPT
... changes within regulatory networks would be cisregulatory (e.g. promoter, enhancer at the gene) • The reason is that cis-regulatory changes would only change the expression of one gene • On the other hand, Trans-regulatory changes are often overly pleiotropic, and thus don’t occur as often. But, whe ...
... changes within regulatory networks would be cisregulatory (e.g. promoter, enhancer at the gene) • The reason is that cis-regulatory changes would only change the expression of one gene • On the other hand, Trans-regulatory changes are often overly pleiotropic, and thus don’t occur as often. But, whe ...
Overexpression of miR165 Affects Apical
... down-regulation of the ATHB-9/PHV, ATHB-14/PHB and ATHB-15 genes, and concomitantly causes an enlargement of shoot apical meristems (SAMs) and an enhancement in vascular development. Here we demonstrated that overexpression of miR165 causes a drastic reduction in the transcript levels of all five HD ...
... down-regulation of the ATHB-9/PHV, ATHB-14/PHB and ATHB-15 genes, and concomitantly causes an enlargement of shoot apical meristems (SAMs) and an enhancement in vascular development. Here we demonstrated that overexpression of miR165 causes a drastic reduction in the transcript levels of all five HD ...
Mutations in a gene encoding a novel protein tyrosine
... Fig. 1 A physical map of the LD critical region at 6q24. a, A YAC contig between D6S1003 and D6S311. The presence of a DNA marker on a YAC clone is shown by a corresponding vertical bar; those highlighted with a circle or a square represent genetic markers or ESTs, respectively, and those remaining ...
... Fig. 1 A physical map of the LD critical region at 6q24. a, A YAC contig between D6S1003 and D6S311. The presence of a DNA marker on a YAC clone is shown by a corresponding vertical bar; those highlighted with a circle or a square represent genetic markers or ESTs, respectively, and those remaining ...
Dosyayı İndir
... Plasmids, such as F factors, which are transmitted via conjugation are termed conjugative plasmids ...
... Plasmids, such as F factors, which are transmitted via conjugation are termed conjugative plasmids ...
Doubling Down on Genomes: Polyploidy and Crop Plants
... Using Brassica (cabbages) allopolyploids, a landmark study detailed the loss and gain of restriction fragments in newly synthesized allopolyploids mimicking natural B. rapa and B. juncea (Song et al., 1995). This study was important as Song and colleagues (1995) were able to use the precise progenit ...
... Using Brassica (cabbages) allopolyploids, a landmark study detailed the loss and gain of restriction fragments in newly synthesized allopolyploids mimicking natural B. rapa and B. juncea (Song et al., 1995). This study was important as Song and colleagues (1995) were able to use the precise progenit ...
Ovule and embryo development, apomixis and fertilization Abdul M
... inner integuments develop normally. The development of the embryo sac is also abnormal leading to sacs containing one and four nuclei. In the ucn mutants a horn-like structure protrudes from the outer integument. The embryo sac is often missing giving rise to semi-sterility. In the sub mutants the o ...
... inner integuments develop normally. The development of the embryo sac is also abnormal leading to sacs containing one and four nuclei. In the ucn mutants a horn-like structure protrudes from the outer integument. The embryo sac is often missing giving rise to semi-sterility. In the sub mutants the o ...
Analysis of Flanking Sequences from Dissociation
... Analysis of the sequences that flank insertions of the Ds element revealed several classes of insertions (Table 1). Useful sequence information has been obtained in 85% of the transposant lines investigated. Analysis of the remaining 15% yielded sequences that correspond to the donor T-DNA construct ...
... Analysis of the sequences that flank insertions of the Ds element revealed several classes of insertions (Table 1). Useful sequence information has been obtained in 85% of the transposant lines investigated. Analysis of the remaining 15% yielded sequences that correspond to the donor T-DNA construct ...
Analysis of Flanking Sequences from Dissociation
... Analysis of the sequences that flank insertions of the Ds element revealed several classes of insertions (Table 1). Useful sequence information has been obtained in 85% of the transposant lines investigated. Analysis of the remaining 15% yielded sequences that correspond to the donor T-DNA construct ...
... Analysis of the sequences that flank insertions of the Ds element revealed several classes of insertions (Table 1). Useful sequence information has been obtained in 85% of the transposant lines investigated. Analysis of the remaining 15% yielded sequences that correspond to the donor T-DNA construct ...
Gene Section RAD52 (RAD52 homolog (S. cerevisiae)) Atlas of Genetics and Cytogenetics
... DNA binding properties are linked to various amino acids, including, Arg-55, Tyr-65, Lys-152, Arg-153, Arg-156. Arg-55 and Lys-152 are necessarily for ssDNA binding, whereas Tyr-65, Arg-152, and Arg-156 are essential for binding both ssDNA and dsDNA (Kagawa et al., 2002). Phe-79 and Lys-102 have als ...
... DNA binding properties are linked to various amino acids, including, Arg-55, Tyr-65, Lys-152, Arg-153, Arg-156. Arg-55 and Lys-152 are necessarily for ssDNA binding, whereas Tyr-65, Arg-152, and Arg-156 are essential for binding both ssDNA and dsDNA (Kagawa et al., 2002). Phe-79 and Lys-102 have als ...
Genome-wide expression analysis of cultured
... BACKGROUND: The pathologic features of Down syndrome are assumed to be the result of over-expression of genes located on chromosome 21 and/or a more global transcriptional misregulation that crosses chromosomal borders. METHODS: To address this issue, four RNA samples from trisomy 21 placentas and f ...
... BACKGROUND: The pathologic features of Down syndrome are assumed to be the result of over-expression of genes located on chromosome 21 and/or a more global transcriptional misregulation that crosses chromosomal borders. METHODS: To address this issue, four RNA samples from trisomy 21 placentas and f ...
Vital Genes in the Heterochromatin of
... Joint efforts are now required to complete the molecular characterization of the heterochromatic genome of D. melanogaster and to extend the work to other Drosophila species. This may allow us to characterize new heterochromatic genes and to learn how they have evolved. An important goal is also to ...
... Joint efforts are now required to complete the molecular characterization of the heterochromatic genome of D. melanogaster and to extend the work to other Drosophila species. This may allow us to characterize new heterochromatic genes and to learn how they have evolved. An important goal is also to ...
Epigenetics - the Houpt Lab
... (A) Individual residues on histone tails undergo a number of unique modifications ...surrounding the transcription start site (TSS) for a given gene. These modifications in turn correlate with transcriptional repression (top), in which DNA is tightly condensed on the nucleosome and therefore inacces ...
... (A) Individual residues on histone tails undergo a number of unique modifications ...surrounding the transcription start site (TSS) for a given gene. These modifications in turn correlate with transcriptional repression (top), in which DNA is tightly condensed on the nucleosome and therefore inacces ...
RNA-Mediated Programming of Developmental
... resulted in terminal deletions, while targeting an internal sequence resulted in heterogeneous internal deletions of the homologous zygotic sequence (23, 27). The sequence specificity of this trans-nuclear effect is therefore likely to be achieved by the pairing of homologous nucleic acids. To recon ...
... resulted in terminal deletions, while targeting an internal sequence resulted in heterogeneous internal deletions of the homologous zygotic sequence (23, 27). The sequence specificity of this trans-nuclear effect is therefore likely to be achieved by the pairing of homologous nucleic acids. To recon ...
Leukaemia Section inv(3)(q21q26)x2 Atlas of Genetics and Cytogenetics in Oncology and Haematology
... hematopoietic stem cells and can inhibit myeloid differentiation. Two alternative forms exists, one generated from EVI1, the other MECOM (MDS1 and EVI1 complex locus) through intergenic splicing with MDS1 (myelodysplasia syndrome 1), a gene located 140 kb upstream of EVI1. DNA/RNA EVI1 has 16 exons, ...
... hematopoietic stem cells and can inhibit myeloid differentiation. Two alternative forms exists, one generated from EVI1, the other MECOM (MDS1 and EVI1 complex locus) through intergenic splicing with MDS1 (myelodysplasia syndrome 1), a gene located 140 kb upstream of EVI1. DNA/RNA EVI1 has 16 exons, ...
exercises - Evolutionary Genomics Group
... Now we will run our own genefinding algorithm on the DNA sequence of the genome. This is very often the same that the publishers of the genome has done. The good thing about running one algorithm on all the genomes is that the results will be standardized which is not the case with published annotat ...
... Now we will run our own genefinding algorithm on the DNA sequence of the genome. This is very often the same that the publishers of the genome has done. The good thing about running one algorithm on all the genomes is that the results will be standardized which is not the case with published annotat ...
Chapter 6 Genetic analysis of two loci
... phenotypic ratio may indicate that one of more of these conditions has not been met. Modified ratios in the progeny of a dihybrid cross can therefore reveal useful information about the genes involved. Linkage is one of the most important reasons for distortion of the ratios expected from independen ...
... phenotypic ratio may indicate that one of more of these conditions has not been met. Modified ratios in the progeny of a dihybrid cross can therefore reveal useful information about the genes involved. Linkage is one of the most important reasons for distortion of the ratios expected from independen ...
The Effects of Zygotic Lethal Mutations on Female Germ
... set (20 out of 48) exhibited maternal effects on oogenesis, embryogenesis, or both. In 13 of this last group, only a few eggs were produced before a progressive deterioration of development occurred. It is suggested that perdurance of the wild-type gene product could produce this result. The third g ...
... set (20 out of 48) exhibited maternal effects on oogenesis, embryogenesis, or both. In 13 of this last group, only a few eggs were produced before a progressive deterioration of development occurred. It is suggested that perdurance of the wild-type gene product could produce this result. The third g ...
Myeloid Leukemias - Current and Future Approaches to Targeted
... These advances in understanding of the cellular and molecular basis of AML have begun to realize significant improvements for less toxic and more effective therapies. The cellular source of AML originates from hematopoietic progenitor or stem cells that acquire genetic defects which in turn lead to ...
... These advances in understanding of the cellular and molecular basis of AML have begun to realize significant improvements for less toxic and more effective therapies. The cellular source of AML originates from hematopoietic progenitor or stem cells that acquire genetic defects which in turn lead to ...
Non-syndromic progressive hearing loss DFNA38 is caused by
... C-terminal domain of wolframin as Ala716Thr. Some of these other amino acid substitutions are likely to be disease alleles; others may be rare neutral variants, analogous to those on the ancestral hearing-associated haplotype in Family C. Deafness is not generally a presenting feature of Wolfram syn ...
... C-terminal domain of wolframin as Ala716Thr. Some of these other amino acid substitutions are likely to be disease alleles; others may be rare neutral variants, analogous to those on the ancestral hearing-associated haplotype in Family C. Deafness is not generally a presenting feature of Wolfram syn ...
Molecular Testing Applications in Coagulation
... VTE and may manifest heparin resistance • Acquired deficiencies of protein C and protein S may occur in association with vitamin K deficiency ...
... VTE and may manifest heparin resistance • Acquired deficiencies of protein C and protein S may occur in association with vitamin K deficiency ...
I. The Effect of Puromycin on the Duplication of DNA*
... The influence of puromycin on the accelera2ion of DNA synthesis.—A number of investigators have demonstrated that puromycin is a highly effective inhibitor of protein synthesis in both intact ani mals and cell-free systems (@, 9, 10, 15, 16). This inhibition results from the blocking of the trans ...
... The influence of puromycin on the accelera2ion of DNA synthesis.—A number of investigators have demonstrated that puromycin is a highly effective inhibitor of protein synthesis in both intact ani mals and cell-free systems (@, 9, 10, 15, 16). This inhibition results from the blocking of the trans ...
Preimplantation genetic diagnosis today
... the genomic template DNA during the initial cycles of PCR (Ray and Handyside, 1996). Raising the temperature in the initial cycles improves the efficiency of denaturation and minimizes but does not eliminate allelic dropout (Figure 4). Fortunately in an autosomal recessive condition, allele dropout ...
... the genomic template DNA during the initial cycles of PCR (Ray and Handyside, 1996). Raising the temperature in the initial cycles improves the efficiency of denaturation and minimizes but does not eliminate allelic dropout (Figure 4). Fortunately in an autosomal recessive condition, allele dropout ...
Bioimage_Informatics_2
... which to study the functions of that gene mutation in cancer, and it gives us an in vitro model to test the effectiveness of drugs that might control or even selectively kill cancer cells with that mutation. In these kinds of experiments, one is said to be “screening” for putative oncogene mutations ...
... which to study the functions of that gene mutation in cancer, and it gives us an in vitro model to test the effectiveness of drugs that might control or even selectively kill cancer cells with that mutation. In these kinds of experiments, one is said to be “screening” for putative oncogene mutations ...
Specialized techniques for site-directed mutagenesis in cyanobacteria
... can be directed to a particular locus. The simplest application is insertional inactivation of a gene, by replacing the gene of interest with an allele that has a heterologous cassette (generally an antibiotic-resistance cassette) inserted within its open reading frame. However, as prokaryotic organ ...
... can be directed to a particular locus. The simplest application is insertional inactivation of a gene, by replacing the gene of interest with an allele that has a heterologous cassette (generally an antibiotic-resistance cassette) inserted within its open reading frame. However, as prokaryotic organ ...
Oncogenomics
Oncogenomics is a relatively new sub-field of genomics that applies high throughput technologies to characterize genes associated with cancer. Oncogenomics is synonymous with ""cancer genomics"". Cancer is a genetic disease caused by accumulation of mutations to DNA leading to unrestrained cell proliferation and neoplasm formation. The goal of oncogenomics is to identify new oncogenes or tumor suppressor genes that may provide new insights into cancer diagnosis, predicting clinical outcome of cancers, and new targets for cancer therapies. The success of targeted cancer therapies such as Gleevec, Herceptin, and Avastin raised the hope for oncogenomics to elucidate new targets for cancer treatment.Besides understanding the underlying genetic mechanisms that initiates or drives cancer progression, one of the main goals of oncogenomics is to allow for the development of personalized cancer treatment. Cancer develops due to an accumulation of mutations in DNA. These mutations accumulate randomly, and thus, different DNA mutations and mutation combinations exist between different individuals with the same type of cancer. Thus, identifying and targeting specific mutations which have occurred in an individual patient may lead to increased efficacy of cancer therapy.The completion of the Human Genome Project has greatly facilitated the field of oncogenomics and has increased the abilities of researchers to find cancer causing genes. In addition, the sequencing technologies now available for sequence generation and data analysis have been applied to the study of oncogenomics. With the amount of research conducted on cancer genomes and the accumulation of databases documenting the mutational changes, it has been predicted that the most important cancer-causing mutations, rearrangements, and altered expression levels will be cataloged and well characterized within the next decade.Cancer research may look either on the genomic level at DNA mutations, the epigenetic level at methylation or histone modification changes, the transcription level at altered levels of gene expression, or the protein level at altered levels of protein abundance and function in cancer cells. Oncogenomics focuses on the genomic, epigenomic, and transcript level alterations in cancer.