Regulation of biosynthesis and transport of aromatic amino acids in
... doe.gov); the partially sequenced genome of Bacillus anthracis was obtained from The Institute for Genomic Research (http://www.tigr.org). The gene names in un¢nished genomes were assigned based on the names of orthologous genes in related species. FASTA sequences of all proteins with new or revised ...
... doe.gov); the partially sequenced genome of Bacillus anthracis was obtained from The Institute for Genomic Research (http://www.tigr.org). The gene names in un¢nished genomes were assigned based on the names of orthologous genes in related species. FASTA sequences of all proteins with new or revised ...
PDF
... similarities. The relationships between this structural organrzation and the expression patterns of the Hox genes during fetal development have been extensively studied and reviewed (e.g. Gaunt, 1991). Briefly, genes located at the 3' extremities of the complexes (such as group 1 or 2 genes) are exp ...
... similarities. The relationships between this structural organrzation and the expression patterns of the Hox genes during fetal development have been extensively studied and reviewed (e.g. Gaunt, 1991). Briefly, genes located at the 3' extremities of the complexes (such as group 1 or 2 genes) are exp ...
gene_prediction_20040930
... 2. Perform database similarity search of expressed sequence tag Sites (EST) database of same organism, or cDNA sequences if available Use gene prediction program to locate genes ...
... 2. Perform database similarity search of expressed sequence tag Sites (EST) database of same organism, or cDNA sequences if available Use gene prediction program to locate genes ...
Single intragenic microsatellite preimplantation
... PCR analysis of amplification efficiency and allele sizes for marker IVS17bTA on blastomeres donated from IVF patients and on diluted DNA of known haplotypes showed that products differing in repeat unit numbers from 7 to 55 TA were reproducibly amplified and detected using the sample preparation an ...
... PCR analysis of amplification efficiency and allele sizes for marker IVS17bTA on blastomeres donated from IVF patients and on diluted DNA of known haplotypes showed that products differing in repeat unit numbers from 7 to 55 TA were reproducibly amplified and detected using the sample preparation an ...
1995 Broad et al: CURRENT STATE OF THE NEW ZEALAND
... which sheep chromosomes to “capture” in different cell fusion experiments (see Burkin et al. 1993). Frequently, only the selected chromosome is retained, but generally a small number of non-selected chromosomes are also retained after the fusion. These can be eliminated by selection as outlined in B ...
... which sheep chromosomes to “capture” in different cell fusion experiments (see Burkin et al. 1993). Frequently, only the selected chromosome is retained, but generally a small number of non-selected chromosomes are also retained after the fusion. These can be eliminated by selection as outlined in B ...
Basic Genetics and Genomics: A Primer for Nurses
... HGP, completed in April of 2003, gave scientists the ability, for the first time, to read the complete genetic blueprint for building a human being (National Human Genome Research Institute [NHGRI], 2008b). As a result of human genome discoveries, it is now known that genetic factors play a role in ...
... HGP, completed in April of 2003, gave scientists the ability, for the first time, to read the complete genetic blueprint for building a human being (National Human Genome Research Institute [NHGRI], 2008b). As a result of human genome discoveries, it is now known that genetic factors play a role in ...
Why Mitochondrial Genes are Most Often Found in Nuclei
... (Felsenstein 1974; Kurland 1992; Lynch 1997; Bergstrom and Pritchard 1998). The details of these two mechanisms are slightly different, but both could, in principle, enhance the mutational load on genes in the mitochondria by reducing both the effective population size and the intensity of purifying ...
... (Felsenstein 1974; Kurland 1992; Lynch 1997; Bergstrom and Pritchard 1998). The details of these two mechanisms are slightly different, but both could, in principle, enhance the mutational load on genes in the mitochondria by reducing both the effective population size and the intensity of purifying ...
Contrasting Properties of Gene-Specific Regulatory, Coding, and
... either the expression or function of a gene product can contribute to phenotypic evolution; mutations that alter gene copy number have also been shown to be an important source of phenotypic variation. Predicting when and why one type of mutation is more likely to underlie a phenotypic change than a ...
... either the expression or function of a gene product can contribute to phenotypic evolution; mutations that alter gene copy number have also been shown to be an important source of phenotypic variation. Predicting when and why one type of mutation is more likely to underlie a phenotypic change than a ...
Tay-Sachs disease
... cultured cells from late-onset patients with various genotypes, treated with two different competitive inhibitors of the enzyme, one being a drug previously approved by the US FDA for another disease which acts as pharmacological chaperones for the mutant enzyme (11,12). Cultured fibroblasts from lat ...
... cultured cells from late-onset patients with various genotypes, treated with two different competitive inhibitors of the enzyme, one being a drug previously approved by the US FDA for another disease which acts as pharmacological chaperones for the mutant enzyme (11,12). Cultured fibroblasts from lat ...
Case Report Section
... carrying gains of 9p have an extra copy of the gene, in its normal or mutated form, leading to a gain of function. The rearrangement here reported, der(9;18)(p10;q10), is rarely detected in patients with PV, myelofibrosis, essential thrombocythemia and therapy-related AML. Some authors suggest that ...
... carrying gains of 9p have an extra copy of the gene, in its normal or mutated form, leading to a gain of function. The rearrangement here reported, der(9;18)(p10;q10), is rarely detected in patients with PV, myelofibrosis, essential thrombocythemia and therapy-related AML. Some authors suggest that ...
Mutation Rates
... Ultimately, the source of genetic variation observed among individuals in populations is gene mutation. Mutation generates new alleles, and these are the substance of all evolutionary change. The mutation rate is defined as the probability that a copy of an allele changes to some other allelic form ...
... Ultimately, the source of genetic variation observed among individuals in populations is gene mutation. Mutation generates new alleles, and these are the substance of all evolutionary change. The mutation rate is defined as the probability that a copy of an allele changes to some other allelic form ...
Legal Liability for Genetic Injuries From Radiation
... mosomes which are comprised of 23 pairs. As to 22 of these pairs, the chromosomes in each pair have the same size, shape, and staining characteristics under the microscope. One of the members of each pair has been contributed by the father and the other by the mother of the individual. Each of these ...
... mosomes which are comprised of 23 pairs. As to 22 of these pairs, the chromosomes in each pair have the same size, shape, and staining characteristics under the microscope. One of the members of each pair has been contributed by the father and the other by the mother of the individual. Each of these ...
Gene Regulation
... architecture of DNA regulatory elements and used them to build an algorithm allowing them to explore the regulatory potential of the human genome. • Although the false positive rate in CRM prediction is likely to be high, the statistical power obtained through a largescale, genome-wide approach reve ...
... architecture of DNA regulatory elements and used them to build an algorithm allowing them to explore the regulatory potential of the human genome. • Although the false positive rate in CRM prediction is likely to be high, the statistical power obtained through a largescale, genome-wide approach reve ...
Biochemistry and Genetics of Tay-Sachs Disease
... on selected asparagine (Asn) residues in the ER. This involves direct transfer of a mannose-rich moiety, Glc3Man9GlcNAc2, via a dolichol intermediate to the growing polypeptide. Removal of the three terminal glucose residues from the high-mannose structures and the initial trimming of one mannose re ...
... on selected asparagine (Asn) residues in the ER. This involves direct transfer of a mannose-rich moiety, Glc3Man9GlcNAc2, via a dolichol intermediate to the growing polypeptide. Removal of the three terminal glucose residues from the high-mannose structures and the initial trimming of one mannose re ...
The Mammalian Mismatch Repair Pathway Removes DNA 8
... cells, in agreement with published data [11]. The effects of Msh2 and Ogg1 inactivation on the steady-state DNA 8-oxoG level were additive, and DNA from msh2⫺/⫺/ ogg1⫺/⫺ MEFs contained 4-fold more 8-oxoG than wildtype MEF DNA (Figure 4A). Inactivation of ogg1 also affected the amount of 8-oxoG in DN ...
... cells, in agreement with published data [11]. The effects of Msh2 and Ogg1 inactivation on the steady-state DNA 8-oxoG level were additive, and DNA from msh2⫺/⫺/ ogg1⫺/⫺ MEFs contained 4-fold more 8-oxoG than wildtype MEF DNA (Figure 4A). Inactivation of ogg1 also affected the amount of 8-oxoG in DN ...
Genes with ectopic expression phenotypes are common, not rare
... loss-of-function phenotype. To the extent that overexpression of genes causes detrimental phenotypes, ectopic or mis-expression is a viable method of identifying genes and the initial determination of the gene function. The GAL4-UAS system developed for Drosophila by Brand and Perrrimon (1993) can b ...
... loss-of-function phenotype. To the extent that overexpression of genes causes detrimental phenotypes, ectopic or mis-expression is a viable method of identifying genes and the initial determination of the gene function. The GAL4-UAS system developed for Drosophila by Brand and Perrrimon (1993) can b ...
Identification and Clustering of Genes Expressed In Circadian
... mouse liver dataset, such that the subsamples contain 75% of the genes in the original dataset. Each of the subsamples are clustered using each clustering algorithm, and the clusterings of the subsamples are compared to each other. The average distance between each clustering is a measurement of sta ...
... mouse liver dataset, such that the subsamples contain 75% of the genes in the original dataset. Each of the subsamples are clustered using each clustering algorithm, and the clusterings of the subsamples are compared to each other. The average distance between each clustering is a measurement of sta ...
Supplementary Data File Supplementary Figures Figure S1
... Table S10. Top ten statistically significant KEGG pathways (adjusted P-value < 0.01) in the two-group cancer versus normal comparisons. We report the top ten pathways or all the significant pathways if there were less than ten for A. AML vs 1000 Genomes, B. GBM vs 1000 Genomes, C. OVC vs 1000 Genom ...
... Table S10. Top ten statistically significant KEGG pathways (adjusted P-value < 0.01) in the two-group cancer versus normal comparisons. We report the top ten pathways or all the significant pathways if there were less than ten for A. AML vs 1000 Genomes, B. GBM vs 1000 Genomes, C. OVC vs 1000 Genom ...
IOSR Journal of Dental and Medical Sciences (IOSR-JDMS)
... It is documented that large intragenic deletions and duplications together account for more than twothirds of the mutations leading to DMD and BMD and, despite heterogeneity in deletion size and location, two hot spots have been identified. Of which, the major one involves exons 40-55 [27, 28]. Thes ...
... It is documented that large intragenic deletions and duplications together account for more than twothirds of the mutations leading to DMD and BMD and, despite heterogeneity in deletion size and location, two hot spots have been identified. Of which, the major one involves exons 40-55 [27, 28]. Thes ...
The neutral theory of molecular
... the probability that this allele will become fixed in the population if (a) it is neutral, (b) it confers a selective advantage of 0.01, or (c) it has a selective disadvantage of 0.001? For simplicity, we assume that Ne = N (=1000). For the neutral case, the probability P = 1 / 2N = 0.05%. From equa ...
... the probability that this allele will become fixed in the population if (a) it is neutral, (b) it confers a selective advantage of 0.01, or (c) it has a selective disadvantage of 0.001? For simplicity, we assume that Ne = N (=1000). For the neutral case, the probability P = 1 / 2N = 0.05%. From equa ...
Does Activation of the TALl Gene Occur in a Majority
... marker. Five micrograms of RNA from each specimen was treated with 2 U of RNase-free DNase1 (Ambion, Austin, TX) and 80 U RNase inhibitor (Promega) in 50 mmoVL sodium acetate, 10 mmol/L MgCI,, and 2 mmol/L CaC1, (final volume, 40 pL) at37°C for 30 minutes. The mixture was extracted once with phenol, ...
... marker. Five micrograms of RNA from each specimen was treated with 2 U of RNase-free DNase1 (Ambion, Austin, TX) and 80 U RNase inhibitor (Promega) in 50 mmoVL sodium acetate, 10 mmol/L MgCI,, and 2 mmol/L CaC1, (final volume, 40 pL) at37°C for 30 minutes. The mixture was extracted once with phenol, ...
Oncogenomics
Oncogenomics is a relatively new sub-field of genomics that applies high throughput technologies to characterize genes associated with cancer. Oncogenomics is synonymous with ""cancer genomics"". Cancer is a genetic disease caused by accumulation of mutations to DNA leading to unrestrained cell proliferation and neoplasm formation. The goal of oncogenomics is to identify new oncogenes or tumor suppressor genes that may provide new insights into cancer diagnosis, predicting clinical outcome of cancers, and new targets for cancer therapies. The success of targeted cancer therapies such as Gleevec, Herceptin, and Avastin raised the hope for oncogenomics to elucidate new targets for cancer treatment.Besides understanding the underlying genetic mechanisms that initiates or drives cancer progression, one of the main goals of oncogenomics is to allow for the development of personalized cancer treatment. Cancer develops due to an accumulation of mutations in DNA. These mutations accumulate randomly, and thus, different DNA mutations and mutation combinations exist between different individuals with the same type of cancer. Thus, identifying and targeting specific mutations which have occurred in an individual patient may lead to increased efficacy of cancer therapy.The completion of the Human Genome Project has greatly facilitated the field of oncogenomics and has increased the abilities of researchers to find cancer causing genes. In addition, the sequencing technologies now available for sequence generation and data analysis have been applied to the study of oncogenomics. With the amount of research conducted on cancer genomes and the accumulation of databases documenting the mutational changes, it has been predicted that the most important cancer-causing mutations, rearrangements, and altered expression levels will be cataloged and well characterized within the next decade.Cancer research may look either on the genomic level at DNA mutations, the epigenetic level at methylation or histone modification changes, the transcription level at altered levels of gene expression, or the protein level at altered levels of protein abundance and function in cancer cells. Oncogenomics focuses on the genomic, epigenomic, and transcript level alterations in cancer.