Roles of DNA Polymerase V and RecA Protein in SOS Damage
... RecA is engaged in three distinct roles in the cell. Its principal role is to catalyze DNA strand pairing, leading to homologous recombination.8 There are, however, two additional roles for RecA. One involves induction of the SOS response, and another is necessary for triggering SOS mutation.3,9 Rec ...
... RecA is engaged in three distinct roles in the cell. Its principal role is to catalyze DNA strand pairing, leading to homologous recombination.8 There are, however, two additional roles for RecA. One involves induction of the SOS response, and another is necessary for triggering SOS mutation.3,9 Rec ...
DNA
... • The structure and the four genomic letters code for all living organisms. • Adenine, guanine, thymine, and cytosine which pair A-T and C-G on complimentary strands. ...
... • The structure and the four genomic letters code for all living organisms. • Adenine, guanine, thymine, and cytosine which pair A-T and C-G on complimentary strands. ...
Fidelity of Primate Cell Repair of a Double
... involve DNA slipped/secondary structures (out-of-register interstrand mispairings at repeat sequences). One hypothesis predicts that expansion events arise from errors at DNA replication forks (10, 11). We have shown that cis-elements such as the location of replication initiation and fork direction ...
... involve DNA slipped/secondary structures (out-of-register interstrand mispairings at repeat sequences). One hypothesis predicts that expansion events arise from errors at DNA replication forks (10, 11). We have shown that cis-elements such as the location of replication initiation and fork direction ...
Recognition of Local DNA Structures by p53 Protein
... local DNA structures and summarize information about interactions of p53 with these structural DNA motifs. These recent data provide important insights into the complexity of the p53 pathway and the functional consequences of wild-type and mutant p53 activation in normal and tumor cells. Keywords: p ...
... local DNA structures and summarize information about interactions of p53 with these structural DNA motifs. These recent data provide important insights into the complexity of the p53 pathway and the functional consequences of wild-type and mutant p53 activation in normal and tumor cells. Keywords: p ...
Structure and function of the GINS complex, a key component of the
... ORC binds to replication origin DNA throughout the cell cycle. In G1 , Cdc6 binds to ORC and recruits the hexameric MCM helicase and Cdt1 to form the pre-RC. Pre-RC assembly is said to licence the origin for replication and can only occur in G1 , thereby preventing re-replication of the same sequenc ...
... ORC binds to replication origin DNA throughout the cell cycle. In G1 , Cdc6 binds to ORC and recruits the hexameric MCM helicase and Cdt1 to form the pre-RC. Pre-RC assembly is said to licence the origin for replication and can only occur in G1 , thereby preventing re-replication of the same sequenc ...
DNA methylation profiling identifies epigenetic dysregulation in
... best-studied epigenetic modification and governs transcriptional regulation and silencing (for review, see Suzuki and Bird, 2008). Unlike the relatively study genome, the methylome changes in a dynamic way during development, tissue differentiation and aging. Pathologically altered DNA methylation is ...
... best-studied epigenetic modification and governs transcriptional regulation and silencing (for review, see Suzuki and Bird, 2008). Unlike the relatively study genome, the methylome changes in a dynamic way during development, tissue differentiation and aging. Pathologically altered DNA methylation is ...
Of mice and (wo)men: genotype–phenotype
... The genotype–phenotype correlations observed at the human BRCA1 gene may also be explained by similar models, as discussed above. Most likely, the primary outcome of loss of BRCA1 function in tumors is, both in mouse and man, the accumulation of DNA damage and the altered transcriptional response to ...
... The genotype–phenotype correlations observed at the human BRCA1 gene may also be explained by similar models, as discussed above. Most likely, the primary outcome of loss of BRCA1 function in tumors is, both in mouse and man, the accumulation of DNA damage and the altered transcriptional response to ...
NUCLEIC ACID ECONOMY IN BACTERIA INFECTED WITH
... survival of 10-~) in chilled centrifuge tubes. After about 30 minutes in an ice bath, the tubes were warmed 30 minutes at 37°C. and refrigerated overnight. The next morning I0 #g. desoxyribonuclease was added to each tube and the contents were diluted to 10 ml. with 0.1 per cent peptone water. After ...
... survival of 10-~) in chilled centrifuge tubes. After about 30 minutes in an ice bath, the tubes were warmed 30 minutes at 37°C. and refrigerated overnight. The next morning I0 #g. desoxyribonuclease was added to each tube and the contents were diluted to 10 ml. with 0.1 per cent peptone water. After ...
Evaluation of genomic DNA from paraffin
... separated by a clear space with oedematous fluid and in some cases there is also a diffuse infiltration of subendocardial fibrosis (Tidholm & Jönsson 2005). In a study, 64 of 65 (98%) dogs with confirmed DCM were positive for attenuated wavy fibers. In 147 dogs with other heart disease than DCM only ...
... separated by a clear space with oedematous fluid and in some cases there is also a diffuse infiltration of subendocardial fibrosis (Tidholm & Jönsson 2005). In a study, 64 of 65 (98%) dogs with confirmed DCM were positive for attenuated wavy fibers. In 147 dogs with other heart disease than DCM only ...
The physics behind the larger scale organization of DNA in eukaryotes
... In this paper, we discuss in detail the organization of chromatin during a cell cycle at several levels. We show that current experimental data on large-scale chromatin organization have not yet reached the level of precision to allow for detailed modeling. We speculate in some detail about the poss ...
... In this paper, we discuss in detail the organization of chromatin during a cell cycle at several levels. We show that current experimental data on large-scale chromatin organization have not yet reached the level of precision to allow for detailed modeling. We speculate in some detail about the poss ...
Read the Nobel Lecture
... of published models for telomere replication. In 1974, James Watson suggested that a linear chromosome should get shorter as cells divided, based on the mechanism by which DNA polymerase replicates DNA during cell division (Watson, 1972). A similar idea was also proposed by Alexi Olovinkov, who sugg ...
... of published models for telomere replication. In 1974, James Watson suggested that a linear chromosome should get shorter as cells divided, based on the mechanism by which DNA polymerase replicates DNA during cell division (Watson, 1972). A similar idea was also proposed by Alexi Olovinkov, who sugg ...
Multiplex in-vitro Detection using SERS
... experimental conditions for FAM- and TAMRA-labelled DNA.78 They found that dyespermine and dye-DNA interactions cause changes in the observed fluorescence intensity, which is also dependant on experimental conditions such as the pH and concentration. It was also observed that these fluctuations in i ...
... experimental conditions for FAM- and TAMRA-labelled DNA.78 They found that dyespermine and dye-DNA interactions cause changes in the observed fluorescence intensity, which is also dependant on experimental conditions such as the pH and concentration. It was also observed that these fluctuations in i ...
Nanopore Unzipping of Individual DNA Hairpin Molecules
... under constant force or constant loading rate. Using a dynamic voltage control method, the entry rate of polynucleotides into the pore and the voltage pattern applied to induce hairpin unzipping are independently set. Thus, hundreds of unzipping events can be tested in a short period of time (few mi ...
... under constant force or constant loading rate. Using a dynamic voltage control method, the entry rate of polynucleotides into the pore and the voltage pattern applied to induce hairpin unzipping are independently set. Thus, hundreds of unzipping events can be tested in a short period of time (few mi ...
The Rad50 Signature Motif: Essential to ATP Binding and
... manganese ions for catalysis and form a DNAbinding groove that can accommodate a doublestranded DNA end.10 In vitro, Mre11 homologs exhibit 30 to 50 exonuclease activity on DNA substrates, as well as endonuclease activity on constrained structures such as hairpin ends.11 – 16 In every organism studi ...
... manganese ions for catalysis and form a DNAbinding groove that can accommodate a doublestranded DNA end.10 In vitro, Mre11 homologs exhibit 30 to 50 exonuclease activity on DNA substrates, as well as endonuclease activity on constrained structures such as hairpin ends.11 – 16 In every organism studi ...
7nQ Jj I f NO "7^07 - UNT Digital Library
... technology, population genetics, and much more. The text is very well written and easy to understand. It offers a detailed look at all the components of genetics. It contains study questions as well as summaries for each chapter to provide an even better way to retain the knowledge from my lectures ...
... technology, population genetics, and much more. The text is very well written and easy to understand. It offers a detailed look at all the components of genetics. It contains study questions as well as summaries for each chapter to provide an even better way to retain the knowledge from my lectures ...
The consequences of Rad51 overexpression for normal and tumor
... phase [31]. This is correlated with an increase in p21, the Cdk inhibitor. When Rad51 expression is reduced by antisense inhibition, the level of p21 decreases and cells become sensitive to etoposide drug treatments that cause double strand breaks. While the relationship between the higher order str ...
... phase [31]. This is correlated with an increase in p21, the Cdk inhibitor. When Rad51 expression is reduced by antisense inhibition, the level of p21 decreases and cells become sensitive to etoposide drug treatments that cause double strand breaks. While the relationship between the higher order str ...
Gene Detection Systems Catalog
... 2. Changes -Orders arising hereunder may be changed or amended only by written agreement signed by both Buyer and Seller, setting forth the particular changes to be made and the effect, if any, of such changes on the price and time of delivery. Buyer may not cancel this order unless such cancellatio ...
... 2. Changes -Orders arising hereunder may be changed or amended only by written agreement signed by both Buyer and Seller, setting forth the particular changes to be made and the effect, if any, of such changes on the price and time of delivery. Buyer may not cancel this order unless such cancellatio ...
Demarcation of coding and non-coding regions of DNA using linear
... These codons give rise to amino acid, which in turn produce proteins. There are totally 20 different amino acids. This gives an implicit meaning that the mapping between codons and the amino acids is a many to one mapping [2]. That is, there are many codons that must code for the same amino acid. Th ...
... These codons give rise to amino acid, which in turn produce proteins. There are totally 20 different amino acids. This gives an implicit meaning that the mapping between codons and the amino acids is a many to one mapping [2]. That is, there are many codons that must code for the same amino acid. Th ...
Towards Programmable Molecular Machines
... Recent related results: Several experimental molecular machines have been recently developed in a laboratory setting. These machines are usually powered either by a natural phenomenon called strand invasion (also known as branch migration) or by enzymes that can break the backbone of DNA at location ...
... Recent related results: Several experimental molecular machines have been recently developed in a laboratory setting. These machines are usually powered either by a natural phenomenon called strand invasion (also known as branch migration) or by enzymes that can break the backbone of DNA at location ...
A genome-wide analysis of DNA methylation in buccal - VU-DARE
... restricting to the most variable CpG sites (for the top 10% CpGs of which methylation level varied most between subjects, the average heritability was 37%) 34. It was also found that gene body and intergenic regions showed higher average methylation levels, more variation between subjects, and highe ...
... restricting to the most variable CpG sites (for the top 10% CpGs of which methylation level varied most between subjects, the average heritability was 37%) 34. It was also found that gene body and intergenic regions showed higher average methylation levels, more variation between subjects, and highe ...
Many ways to telomere dysfunction: in vivo studies using
... chromosomes was ®rst deduced in the 1930s by Herman MuÈller (MuÈller, 1938), who showed that X-irradiation of Drosophila rarely resulted in terminal deletions or inversions of chromosomes, suggesting that chromosome ends have protective structures that distinguish them from broken chromosomes, which ...
... chromosomes was ®rst deduced in the 1930s by Herman MuÈller (MuÈller, 1938), who showed that X-irradiation of Drosophila rarely resulted in terminal deletions or inversions of chromosomes, suggesting that chromosome ends have protective structures that distinguish them from broken chromosomes, which ...
Distortion of quantitative genomic and expression
... regarding reproducibility of these techniques have been raised by cross-validation studies in different laboratories (1–5). Strategies to mitigate variability in the results obtained from replicate studies have focused on standardizing technical factors, such as array production, RNA synthesis, labe ...
... regarding reproducibility of these techniques have been raised by cross-validation studies in different laboratories (1–5). Strategies to mitigate variability in the results obtained from replicate studies have focused on standardizing technical factors, such as array production, RNA synthesis, labe ...