Dissection of molecular interactions of replication protein A in
... ensures that the appropriate pathway correctly processes them. The ssDNA-binding activity of RPA is primarily mediated by two high-affinity domains in the RPA1 subunit. DNA binds to these domains by interacting with polar and aromatic residues in a DNAbinding cleft in each domain. The aromatic resid ...
... ensures that the appropriate pathway correctly processes them. The ssDNA-binding activity of RPA is primarily mediated by two high-affinity domains in the RPA1 subunit. DNA binds to these domains by interacting with polar and aromatic residues in a DNAbinding cleft in each domain. The aromatic resid ...
p53, the Cellular Gatekeeper Review for Growth and Division
... but some evidence has suggested that ubiquitin-mediated proteolysis plays a role. In addition to this low protein concentration, in some cells p53 probably also exists in a latent form, inactive for transcription. Under these conditions, the p53 protein must receive a signal or alteration to activat ...
... but some evidence has suggested that ubiquitin-mediated proteolysis plays a role. In addition to this low protein concentration, in some cells p53 probably also exists in a latent form, inactive for transcription. Under these conditions, the p53 protein must receive a signal or alteration to activat ...
Are Restriction Enzymes Recognition Sites Underrepresented in the
... against invading phages. Restriction modification system consists of a restriction enzyme and a methyltransferase enzyme that work in a complementary fashion to cut foreign DNA and at the same time methylates and protects host DNA. Recognition site sequence is usually specific for each restriction e ...
... against invading phages. Restriction modification system consists of a restriction enzyme and a methyltransferase enzyme that work in a complementary fashion to cut foreign DNA and at the same time methylates and protects host DNA. Recognition site sequence is usually specific for each restriction e ...
Molecular mechanics of the interactions of spermine with DNA: DNA
... diaminobutane moiety into a fully extended conformation and produces a single distance (approximately 6.3 A) between the secondary amino groups (data not shown). The aminopropyl groups attached to the secondary amino groups assume a variety of conformations, however, and there are a variety of dista ...
... diaminobutane moiety into a fully extended conformation and produces a single distance (approximately 6.3 A) between the secondary amino groups (data not shown). The aminopropyl groups attached to the secondary amino groups assume a variety of conformations, however, and there are a variety of dista ...
Telomere maintenance without telomerase
... Although the majority of yeast cells die in the absence of telomerase, Lundblad and Blackburn (1993) observed that a small subpopulation could escape the lethal consequences of a telomerase defect. The rare survivors recovered from an est1-D strain not only regained the ability to grow but also disp ...
... Although the majority of yeast cells die in the absence of telomerase, Lundblad and Blackburn (1993) observed that a small subpopulation could escape the lethal consequences of a telomerase defect. The rare survivors recovered from an est1-D strain not only regained the ability to grow but also disp ...
Epigenetic Regulation ofbdnfGene Transcription in the
... was used to determine the DNA methylation status of the bdnf gene. Methylation-specific PCR primers were designed using Methprimer software (http://www.urogene.org/methprimer/) (Li and Dahiya, 2002). CpG islands were found within promoters 1 and 2 and the exonic regions of the bdnf gene immediately ...
... was used to determine the DNA methylation status of the bdnf gene. Methylation-specific PCR primers were designed using Methprimer software (http://www.urogene.org/methprimer/) (Li and Dahiya, 2002). CpG islands were found within promoters 1 and 2 and the exonic regions of the bdnf gene immediately ...
Fanconi anemia and RAD50 deficiency: genetic and functional
... Basic research involving human chromosomal instability and cancer susceptibility syndromes is necessary to gain insights into molecular mechanisms which maintain genomic integrity. The focus of this thesis is directed at the molecular, cellular and clinical aspects of two groups of inherited disease ...
... Basic research involving human chromosomal instability and cancer susceptibility syndromes is necessary to gain insights into molecular mechanisms which maintain genomic integrity. The focus of this thesis is directed at the molecular, cellular and clinical aspects of two groups of inherited disease ...
DNA
... It is a threadlike structure found in the nucleus of the cell which is made from a long strand of DNA. Different organisms have a different number of chromosomes in their cells. • Thomas Morgan(1920s) - Evidence that genes are located on chromosomes was discovered by genetic experiments performed wi ...
... It is a threadlike structure found in the nucleus of the cell which is made from a long strand of DNA. Different organisms have a different number of chromosomes in their cells. • Thomas Morgan(1920s) - Evidence that genes are located on chromosomes was discovered by genetic experiments performed wi ...
Primer on Molecular Biology
... It is a threadlike structure found in the nucleus of the cell which is made from a long strand of DNA. Different organisms have a different number of chromosomes in their cells. • Thomas Morgan(1920s) - Evidence that genes are located on chromosomes was discovered by genetic experiments performed wi ...
... It is a threadlike structure found in the nucleus of the cell which is made from a long strand of DNA. Different organisms have a different number of chromosomes in their cells. • Thomas Morgan(1920s) - Evidence that genes are located on chromosomes was discovered by genetic experiments performed wi ...
UCSD_PevznerMolecula.. - Purdue University :: Computer Science
... It is a threadlike structure found in the nucleus of the cell which is made from a long strand of DNA. Different organisms have a different number of chromosomes in their cells. • Thomas Morgan(1920s) - Evidence that genes are located on chromosomes was discovered by genetic experiments performed wi ...
... It is a threadlike structure found in the nucleus of the cell which is made from a long strand of DNA. Different organisms have a different number of chromosomes in their cells. • Thomas Morgan(1920s) - Evidence that genes are located on chromosomes was discovered by genetic experiments performed wi ...
Primer on Molecular Biology
... It is a threadlike structure found in the nucleus of the cell which is made from a long strand of DNA. Different organisms have a different number of chromosomes in their cells. • Thomas Morgan(1920s) - Evidence that genes are located on chromosomes was discovered by genetic experiments performed wi ...
... It is a threadlike structure found in the nucleus of the cell which is made from a long strand of DNA. Different organisms have a different number of chromosomes in their cells. • Thomas Morgan(1920s) - Evidence that genes are located on chromosomes was discovered by genetic experiments performed wi ...
Resolving Individuals Contributing Trace Amounts of DNA to Highly
... components of the mixture [8]. Nevertheless, these methods based on STRs expectedly suffer from limited power when using severely degraded DNA [8,9]. Mitochondrial DNA (mtDNA) based on hypervariable region sequencing is useful when analyzing degraded DNA due to its high copy number and improved stab ...
... components of the mixture [8]. Nevertheless, these methods based on STRs expectedly suffer from limited power when using severely degraded DNA [8,9]. Mitochondrial DNA (mtDNA) based on hypervariable region sequencing is useful when analyzing degraded DNA due to its high copy number and improved stab ...
arXiv:0708.2724v1 [cond-mat.other] 20 Aug 2007
... Step (4) is the post-processing of the sequence data, which involves the reassembly of the short sequences to get the complete sequence of the original strand of DNA. Because of this, the short strands of DNA are required to have large overlapping sequences in order to match them ...
... Step (4) is the post-processing of the sequence data, which involves the reassembly of the short sequences to get the complete sequence of the original strand of DNA. Because of this, the short strands of DNA are required to have large overlapping sequences in order to match them ...
Molecular Identification of Nematodes Manual
... *Denaturation was explained above. Its very important to get all the double-stranded DNA templates into single-stranded form, especially during the first several cycles of the main file. (What gets amplified and what does not during these first several cycles greatly influences the final outcome of ...
... *Denaturation was explained above. Its very important to get all the double-stranded DNA templates into single-stranded form, especially during the first several cycles of the main file. (What gets amplified and what does not during these first several cycles greatly influences the final outcome of ...
Localization and structural analysis of the ribosomal RNA operons of
... identified and appeared to contain a portion of an rRNA operon (P. Hallenbeck and S. Kaplan, unpublished results). When a subclone of the lambda phage was utilized as a probe in Southern hybridizations of/?, sphaeroides bulk genomic DNA, three strong signals were identified in two different restrict ...
... identified and appeared to contain a portion of an rRNA operon (P. Hallenbeck and S. Kaplan, unpublished results). When a subclone of the lambda phage was utilized as a probe in Southern hybridizations of/?, sphaeroides bulk genomic DNA, three strong signals were identified in two different restrict ...
Chance and Necessity in Arthur Peacocke`s Scientific Work
... time understood it. Such thoughts must have started to be formed in the mind of Peacocke and lead him on the path of pondering the nature of the interaction of living matter with the non-living world and communion of the whole of creation. At the very root of his subsequent deep thoughts on the natu ...
... time understood it. Such thoughts must have started to be formed in the mind of Peacocke and lead him on the path of pondering the nature of the interaction of living matter with the non-living world and communion of the whole of creation. At the very root of his subsequent deep thoughts on the natu ...
DNA Methylation Analysis
... converted DNA is interrogated simultaneously at each locus by two probes, one designed against the unmethylated site and one against the methylated site. After hybridization, allele-specific single-base extension provides another level of specificity and incorporates a fluorescent label for detectio ...
... converted DNA is interrogated simultaneously at each locus by two probes, one designed against the unmethylated site and one against the methylated site. After hybridization, allele-specific single-base extension provides another level of specificity and incorporates a fluorescent label for detectio ...
Supplementary Materials and methods (doc 154K)
... assessed. We used the protocol previously described by Itoh et al. for the mobilization of plasmid pNI111 by RP4 (Itoh et al 1991). P. aeruginosa PAO1-GFP (resistant to gentamycin and chloramphenicol) was used as a recipient strain. Mating experiments were performed three independent times on LB aga ...
... assessed. We used the protocol previously described by Itoh et al. for the mobilization of plasmid pNI111 by RP4 (Itoh et al 1991). P. aeruginosa PAO1-GFP (resistant to gentamycin and chloramphenicol) was used as a recipient strain. Mating experiments were performed three independent times on LB aga ...
ARTICLES - Weizmann Institute of Science
... Eukaryotic genomes are packaged into nucleosome particles that occlude the DNA from interacting with most DNA binding proteins. Nucleosomes have higher affinity for particular DNA sequences, reflecting the ability of the sequence to bend sharply, as required by the nucleosome structure. However, it ...
... Eukaryotic genomes are packaged into nucleosome particles that occlude the DNA from interacting with most DNA binding proteins. Nucleosomes have higher affinity for particular DNA sequences, reflecting the ability of the sequence to bend sharply, as required by the nucleosome structure. However, it ...
rec-mediated recombinational hot spot activity in bacteriophage
... I n order to survey die entire A chromosome in a single experiment for a possible recombinational hot spot, crosses are performed which in this manuscript are termed “hot spot survey crosses.” These crosses are carried out using conditions which block nearly all DNA synthesis (MCMILINand Russo 1972) ...
... I n order to survey die entire A chromosome in a single experiment for a possible recombinational hot spot, crosses are performed which in this manuscript are termed “hot spot survey crosses.” These crosses are carried out using conditions which block nearly all DNA synthesis (MCMILINand Russo 1972) ...
Replication of damaged DNA in vitro is blocked
... interactions of PAb421 with the p53 C-terminus can in some cases stimulate its ability to bind to DNA (41). It has also been shown that p53 puri®ed in the absence of reducing agents (HA±p53 [¯u, ±DTT]) is inert in DNA binding (39,42,43). In contrast, as is well-documented, p53 lacking the C-terminus ...
... interactions of PAb421 with the p53 C-terminus can in some cases stimulate its ability to bind to DNA (41). It has also been shown that p53 puri®ed in the absence of reducing agents (HA±p53 [¯u, ±DTT]) is inert in DNA binding (39,42,43). In contrast, as is well-documented, p53 lacking the C-terminus ...