Construction of an arabidopsis BAC library and isolation of clones
... clones containing the ATTS0477 sequence, and ATTS0477-1ike sequences. We wished to identify the latter clones because many disease-resistance genes are members of clustered multi-gene families (Martin et al., 1993; Song et al., 1995), and we wished to determine if ATTS0477 was similar in this regard ...
... clones containing the ATTS0477 sequence, and ATTS0477-1ike sequences. We wished to identify the latter clones because many disease-resistance genes are members of clustered multi-gene families (Martin et al., 1993; Song et al., 1995), and we wished to determine if ATTS0477 was similar in this regard ...
The presence of two UvrB subunits in the UvrAB complex ensures
... DNA complexes (Verhoeven et al., 2001). Contour length analysis showed that the DNA is still wrapped around the UvrB protein in these preincision complexes as long as UvrB has bound ATP (Verhoeven et al., 2001). The calculated size of a potential UvrB dimer (152 kDa) is almost exactly the size of th ...
... DNA complexes (Verhoeven et al., 2001). Contour length analysis showed that the DNA is still wrapped around the UvrB protein in these preincision complexes as long as UvrB has bound ATP (Verhoeven et al., 2001). The calculated size of a potential UvrB dimer (152 kDa) is almost exactly the size of th ...
Analysis of the DNA microarray hybridization images using
... further analysis, user is asked about some parameters of tested picture (amount of spots, position of spot in group, distance between spots etc ).This solution, despite that is frequently used in commercial applications where it fulfills expectations, stimulate engineers and scientists to create alg ...
... further analysis, user is asked about some parameters of tested picture (amount of spots, position of spot in group, distance between spots etc ).This solution, despite that is frequently used in commercial applications where it fulfills expectations, stimulate engineers and scientists to create alg ...
Supporting Protocols
... Construction of strains. All class C mutant alleles were transferred into the RAD52 locus in W1588-4C from their original pWJ1086 context, see [2] by a PCR based method [3]. The rad52 sequence in all rad52 class C mutant strains were extended in frame by the gene encoding CFP or YFP. This was done b ...
... Construction of strains. All class C mutant alleles were transferred into the RAD52 locus in W1588-4C from their original pWJ1086 context, see [2] by a PCR based method [3]. The rad52 sequence in all rad52 class C mutant strains were extended in frame by the gene encoding CFP or YFP. This was done b ...
Effects of Salt Concentrations and Bending Energy on the Extent of
... spermine (14) ions by measuring the effect of these cations on the osmotic pressure inhibition of DNA ejection from l-phage. We also carry out complementary measurements of interaxial spacings in bulk DNA solutions in equilibrium with the same osmotic pressure and involving the same salts. We find t ...
... spermine (14) ions by measuring the effect of these cations on the osmotic pressure inhibition of DNA ejection from l-phage. We also carry out complementary measurements of interaxial spacings in bulk DNA solutions in equilibrium with the same osmotic pressure and involving the same salts. We find t ...
A new type of papillomavirus DNA, its presence in genital
... copy number (-50-100 copies per diploid cell). They are closely related to each other as shown by cross-hybridization under stringent conditions (Figure 2). No hybridization was noted with DNA from human embryonic fibroblasts (Figure 2, lane c) even at a sensitivity permitting the detection of less ...
... copy number (-50-100 copies per diploid cell). They are closely related to each other as shown by cross-hybridization under stringent conditions (Figure 2). No hybridization was noted with DNA from human embryonic fibroblasts (Figure 2, lane c) even at a sensitivity permitting the detection of less ...
Elg1 forms an alternative RFC complex important for DNA
... analysis. The result of these screens is presented as a genetic interaction network in Figure 1A, with tetrad analysis of the elg1D interactions shown in Figure 1B. Each line on the network represents a synthetic lethal or synthetic sick (slow growth) interaction between the linked genes. Consistent ...
... analysis. The result of these screens is presented as a genetic interaction network in Figure 1A, with tetrad analysis of the elg1D interactions shown in Figure 1B. Each line on the network represents a synthetic lethal or synthetic sick (slow growth) interaction between the linked genes. Consistent ...
Replication origin plasticity, Taylor-made: inhibition vs
... these experiments, cells were first enriched in early S-phase (albeit not necessarily at the very beginning of S) and then briefly pulse-labeled with IdU, followed by a pulse label with a combination of CldU and HU. In this way, replication forks that were active before replication stress (HU) are t ...
... these experiments, cells were first enriched in early S-phase (albeit not necessarily at the very beginning of S) and then briefly pulse-labeled with IdU, followed by a pulse label with a combination of CldU and HU. In this way, replication forks that were active before replication stress (HU) are t ...
viruses
... are attributable to viral infection [12] and viruses are now second only to smoking as a leading cause of ...
... are attributable to viral infection [12] and viruses are now second only to smoking as a leading cause of ...
The Roles of the Quorum-Sensing System in the Release of
... reaction for PAO-JP2 when it was exposed to a mAb against P. aeruginosa serotype O5 (data not shown). However, a reaction to the mAb was detected in the PAO-JP2 samples cultured with C12-HSL. This band was not detected when C4HSL was used instead of C12-HSL. These results indicate that when the las ...
... reaction for PAO-JP2 when it was exposed to a mAb against P. aeruginosa serotype O5 (data not shown). However, a reaction to the mAb was detected in the PAO-JP2 samples cultured with C12-HSL. This band was not detected when C4HSL was used instead of C12-HSL. These results indicate that when the las ...
Spontaneous mutagenesis in exponentially growing and sta
... revertants), and those which arose between the 4th and the 10th day (stationary-phase revertants). The results of these tests are summarized in Table 3. Generally, in AB1157 strains Arg+ growth-dependent revertants were the result of supB and supL suppressor formation due to GC®AT transitions and AT ...
... revertants), and those which arose between the 4th and the 10th day (stationary-phase revertants). The results of these tests are summarized in Table 3. Generally, in AB1157 strains Arg+ growth-dependent revertants were the result of supB and supL suppressor formation due to GC®AT transitions and AT ...
- Wiley Online Library
... RNA as a guide to cleave and thereby degrade complementary mRNAs. Many discoveries have been made with RNAi-based screening, using small interfering RNAs directly or DNA-encoded transcriptional units that produce short hairpin RNAs (shRNAs) or long microRNA-like transcripts. The technology has conti ...
... RNA as a guide to cleave and thereby degrade complementary mRNAs. Many discoveries have been made with RNAi-based screening, using small interfering RNAs directly or DNA-encoded transcriptional units that produce short hairpin RNAs (shRNAs) or long microRNA-like transcripts. The technology has conti ...
Roles of BRCA1 and BRCA2 in homologous recombination, DNA
... approximately 385 kDa molecular weight (Figure 1). BRCA2 is characterized by a very large exon 11, which encodes peptide motifs required for interaction with the RAD51 protein (Chen et al., 1999b). Together, mutations in BRCA1 and BRCA2 account for the majority of families with hereditary susceptibi ...
... approximately 385 kDa molecular weight (Figure 1). BRCA2 is characterized by a very large exon 11, which encodes peptide motifs required for interaction with the RAD51 protein (Chen et al., 1999b). Together, mutations in BRCA1 and BRCA2 account for the majority of families with hereditary susceptibi ...
PDF of article
... PvuRts1I family enzymes are classified as bacterial type IV modification-dependent restriction endonucleases and they are known to play an important role in defence against phage infection (Loenen & Raleigh, 2014). Several restriction endonucleases, such as McrBC, SauUSI and MspJI, have the ability ...
... PvuRts1I family enzymes are classified as bacterial type IV modification-dependent restriction endonucleases and they are known to play an important role in defence against phage infection (Loenen & Raleigh, 2014). Several restriction endonucleases, such as McrBC, SauUSI and MspJI, have the ability ...
definitive non definitive non-invasive invasive prenatal diagnosis
... • Cannot distinguish type of aneuploidy (e.g., ...
... • Cannot distinguish type of aneuploidy (e.g., ...
SURVEY AND SUMMARY Nucleolar responses to DNA double
... forks collapse. In addition, DSBs can form in response to certain exogenous clastogens such as ionizing radiation (IR) or radiomimetic drugs. In some cases DSBs are formed as a part of a normal physiological program, for example during V(D)J and class switch recombination in developing lymphocytes ( ...
... forks collapse. In addition, DSBs can form in response to certain exogenous clastogens such as ionizing radiation (IR) or radiomimetic drugs. In some cases DSBs are formed as a part of a normal physiological program, for example during V(D)J and class switch recombination in developing lymphocytes ( ...
Does premature aging of the mtDNA mutator mouse prove that
... shortened lifespan. The authors of the reports concluded that their data provided a ‘causative link between mtDNA mutations and aging phenotypes in mammals’ (Trifunovic et al., 2004) and that ‘mtDNA mutations … may be a central mechanism driving mammalian aging’ (Kujoth et al., 2005). Intact mitocho ...
... shortened lifespan. The authors of the reports concluded that their data provided a ‘causative link between mtDNA mutations and aging phenotypes in mammals’ (Trifunovic et al., 2004) and that ‘mtDNA mutations … may be a central mechanism driving mammalian aging’ (Kujoth et al., 2005). Intact mitocho ...
GeNeViSTA Coffin Siris Syndrome: A Disorder of SWI/SNF Pathway
... of the SWI/SNF and flSC complexes are highly conserved, but their compositions are not identical, re ecting an increasing complexity of chromatin through evolution. SWI/SNF and flSC complexes in higher eukaryotes maintain core components to maintain overall shape and remodeling activity and also subst ...
... of the SWI/SNF and flSC complexes are highly conserved, but their compositions are not identical, re ecting an increasing complexity of chromatin through evolution. SWI/SNF and flSC complexes in higher eukaryotes maintain core components to maintain overall shape and remodeling activity and also subst ...
Cunningham Cunningham An Exploration of Bacterial
... of oligonucleotide primers, nucleotides, and taq polymerase, and then heated up. The increased temperature caused the DNA to split into two different complementary strands, which primers and nucleotides are added to by taq polymerase to form new DNA fragments. Eventually, millions of new, identical ...
... of oligonucleotide primers, nucleotides, and taq polymerase, and then heated up. The increased temperature caused the DNA to split into two different complementary strands, which primers and nucleotides are added to by taq polymerase to form new DNA fragments. Eventually, millions of new, identical ...