GENtle, a free multi-purpose molecular biology tool
... theory in the shape of the (personal) computer, this ...
... theory in the shape of the (personal) computer, this ...
Localization and structural analysis of the ribosomal RNA operons of
... feature in the anticodon stem, where an A-U base-pair and not a G-C base-pair was observed in the middle of the stem. In E. coli and Bacillus subtilis this position is occupied by a third G-C base-pair which is required for recognition by the E. coli formylating enzyme (30). DNA sequence comparisons ...
... feature in the anticodon stem, where an A-U base-pair and not a G-C base-pair was observed in the middle of the stem. In E. coli and Bacillus subtilis this position is occupied by a third G-C base-pair which is required for recognition by the E. coli formylating enzyme (30). DNA sequence comparisons ...
In the near future, EU thresholds for adventitious
... Impurity testing in seeds has traditionally been done using either single or sequential seed-pool tests. From an analytical seed sample (e.g. 3000 seeds) equal seed pools are made and analysed individually for the trait of interest. Using binomial statistics, the number of seed pools positive for th ...
... Impurity testing in seeds has traditionally been done using either single or sequential seed-pool tests. From an analytical seed sample (e.g. 3000 seeds) equal seed pools are made and analysed individually for the trait of interest. Using binomial statistics, the number of seed pools positive for th ...
Supplementary Materials and methods (doc 154K)
... 50% PAO1-GFP. Competitions starting at 50% of each strain maximize the ability to detect small differences in fitness. The exact initial proportions were confirmed via flow cytometry (see conditions below). Mixtures were diluted 200-fold in fresh LB and competed for 16 hours at 37°C with no agitatio ...
... 50% PAO1-GFP. Competitions starting at 50% of each strain maximize the ability to detect small differences in fitness. The exact initial proportions were confirmed via flow cytometry (see conditions below). Mixtures were diluted 200-fold in fresh LB and competed for 16 hours at 37°C with no agitatio ...
Variation 3.3
... and her family are your patients. Just before her twins, Sondra and Jason, were born, Ms. Lindsey’s husband, also African-American, died in an automobile accident. His parents were physiologically normal, but he had a brother who died of sickle cell disease at the age of 19. Ms. Lindsey explains to ...
... and her family are your patients. Just before her twins, Sondra and Jason, were born, Ms. Lindsey’s husband, also African-American, died in an automobile accident. His parents were physiologically normal, but he had a brother who died of sickle cell disease at the age of 19. Ms. Lindsey explains to ...
Molecular Biology and Applied Genetics
... gene/protein does, the resulting conclusions are much stronger than if one only use one of these strategies. ...
... gene/protein does, the resulting conclusions are much stronger than if one only use one of these strategies. ...
Pdf - Text of NPTEL IIT Video Lectures
... another thereby it covalent links the D N A fragment together during D N A replication process. Say summary the D N A replication process involves the D N A double helix that unwind at the replication fork. The two single strands are produced, which serve as template for the polymerization of free n ...
... another thereby it covalent links the D N A fragment together during D N A replication process. Say summary the D N A replication process involves the D N A double helix that unwind at the replication fork. The two single strands are produced, which serve as template for the polymerization of free n ...
1: Summary and Options
... aspects of the mutagenic process, for example, to examine how mutagens act on DNA and to study effects of varying doses and rates of exposure to mutagenic agents administered either singly or in combination. Experimentation with animals is essential for assessing potential hazards of new chemical an ...
... aspects of the mutagenic process, for example, to examine how mutagens act on DNA and to study effects of varying doses and rates of exposure to mutagenic agents administered either singly or in combination. Experimentation with animals is essential for assessing potential hazards of new chemical an ...
mMESSAGE mMACHINE® Kit User Guide
... mMESSAGE mMACHINE® Kits are designed for the in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5' end. mMESSAGE mMACHINE® Kit reactions include cap analog [m7G(5')ppp(5')G] in an ultra hi ...
... mMESSAGE mMACHINE® Kits are designed for the in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5' end. mMESSAGE mMACHINE® Kit reactions include cap analog [m7G(5')ppp(5')G] in an ultra hi ...
Finishing the Human Genome
... After Cycle 3 After N cycles, amount of target DNA is 2N-2N Doug Brutlag 2011 ...
... After Cycle 3 After N cycles, amount of target DNA is 2N-2N Doug Brutlag 2011 ...
Inhibition of RNA Synthesis by Anthracycline Analogs
... and inactivate its template function essential for nucleic acid synthesis. Recently, several structural analogs of these antineoplastic agents have been synthesized and some of them were shown to be considerably more effective against experimental tumors than the parent compounds. The majority of re ...
... and inactivate its template function essential for nucleic acid synthesis. Recently, several structural analogs of these antineoplastic agents have been synthesized and some of them were shown to be considerably more effective against experimental tumors than the parent compounds. The majority of re ...
From the Department of Zoology, University of
... of these units per chromosome has not been determined yet, there is evidence that it may not be the same in all species (9, 10). For some time now the nature of the longitudinal differentiation of chromosomes into chromomeres, interchromomeric fibers, hetero-, and euchromatin has been debated. Some ...
... of these units per chromosome has not been determined yet, there is evidence that it may not be the same in all species (9, 10). For some time now the nature of the longitudinal differentiation of chromosomes into chromomeres, interchromomeric fibers, hetero-, and euchromatin has been debated. Some ...
Jigsaw handout - the Biology Scholars Program Wiki
... lactose. When lactose is not present, the proteins encoded by the genes are not needed and the operon is not transcribed. When lactose is present, the gene products are needed and the operon is transcribed. The system is controlled by a protein call lac repressor. Lac repressor is a DNA binding prot ...
... lactose. When lactose is not present, the proteins encoded by the genes are not needed and the operon is not transcribed. When lactose is present, the gene products are needed and the operon is transcribed. The system is controlled by a protein call lac repressor. Lac repressor is a DNA binding prot ...
Document
... • treatment of chromatin with micrococcal nuclease preferentially cuts between the beads ...
... • treatment of chromatin with micrococcal nuclease preferentially cuts between the beads ...
Science and the Dead - Council for British Archaeology
... may also be relevant to destructive sampling of other classes of archaeological remains, particularly faunal remains. In keeping with APABE’s remit, the scope of this document is restricted to remains over 100 years old (herein termed archaeological) from burial sites in England. The focus is on ske ...
... may also be relevant to destructive sampling of other classes of archaeological remains, particularly faunal remains. In keeping with APABE’s remit, the scope of this document is restricted to remains over 100 years old (herein termed archaeological) from burial sites in England. The focus is on ske ...
Transcription
... a general non-specific affinity for DNA, which is referred to as loose binding that is fairly stable. • The addition of s factor to the core enzyme markedly reduces the holoenzyme affinity for non-specific binding by 20 000-fold, and enhances the holoenzyme binding to correct promoter sites 100 time ...
... a general non-specific affinity for DNA, which is referred to as loose binding that is fairly stable. • The addition of s factor to the core enzyme markedly reduces the holoenzyme affinity for non-specific binding by 20 000-fold, and enhances the holoenzyme binding to correct promoter sites 100 time ...
... were isolated with a tightly linked RAPD marker, and subsequently the regions flanking the Rps1 locus were nearly saturated with AFLP markers. Identification and mapping of RAPD markers linked to the Rps1 locus. The screening of 400 decamer primers against the cultivar Williams (rps1-k) and its NIL ...
Aberrant DNA methylation in cancer: potential clinical
... tumour was first reported in 1986 (Ref. 19). However, it was not until recently, particularly following the advent of polymerase chain reaction (PCR)-based techniques for methylation analysis (Ref. 20), that analysis of CpG island methylation in tumours has become widespread. In the past few years, ...
... tumour was first reported in 1986 (Ref. 19). However, it was not until recently, particularly following the advent of polymerase chain reaction (PCR)-based techniques for methylation analysis (Ref. 20), that analysis of CpG island methylation in tumours has become widespread. In the past few years, ...
Evaluation of genomic DNA from paraffin
... Dogs that are clinically diagnosed with DCM reveal two distinct histological forms of DCM. Cardiomyopathy of Boxers and Doberman Pinschers are called “fatty infiltration-degenerative” type and in many giant, large- and medium-sized breeds DCM can be classified as “attenuated wavy fiber” type. Attenu ...
... Dogs that are clinically diagnosed with DCM reveal two distinct histological forms of DCM. Cardiomyopathy of Boxers and Doberman Pinschers are called “fatty infiltration-degenerative” type and in many giant, large- and medium-sized breeds DCM can be classified as “attenuated wavy fiber” type. Attenu ...
The Mobile Genetic Element Alu in the Human Genome
... repetitive elements When the DNA double helix of higher organisms (species other than bacteria and viruses) is split open or denatured into two single strands by heat and left to spontaneously reanneal, the entire DNA is not likely to become double-stranded at the same rate. In contrast, all parts o ...
... repetitive elements When the DNA double helix of higher organisms (species other than bacteria and viruses) is split open or denatured into two single strands by heat and left to spontaneously reanneal, the entire DNA is not likely to become double-stranded at the same rate. In contrast, all parts o ...
Homologous Recombination Between Episomal Plasmids and Chromosomes in Yeast.
... mitotic recombination between closely linked markers. This result was reproduced in the mitotic crosses in Table 3, where factors of increase of more than tenfold relative to the spontaneous rate were found. However, the stimulation of recombination between plasmid and chromosome by the same doses o ...
... mitotic recombination between closely linked markers. This result was reproduced in the mitotic crosses in Table 3, where factors of increase of more than tenfold relative to the spontaneous rate were found. However, the stimulation of recombination between plasmid and chromosome by the same doses o ...
Unusual mutations in high functioning fragile X males
... (fig 1). The proband (IV. 3) was the only affected male in this family. He was diagnosed at the age of 3 years when he presented a typical infantile phenotype of fragile X syndrome (fig 3A). His two sisters (IV4, IV.5) also had full mutations and were reported to be affected Table 1 Repeat sizes (gi ...
... (fig 1). The proband (IV. 3) was the only affected male in this family. He was diagnosed at the age of 3 years when he presented a typical infantile phenotype of fragile X syndrome (fig 3A). His two sisters (IV4, IV.5) also had full mutations and were reported to be affected Table 1 Repeat sizes (gi ...
Nucleic acid double helix
In molecular biology, the term double helix refers to the structure formed by double-stranded molecules of nucleic acids such as DNA. The double helical structure of a nucleic acid complex arises as a consequence of its secondary structure, and is a fundamental component in determining its tertiary structure. The term entered popular culture with the publication in 1968 of The Double Helix: A Personal Account of the Discovery of the Structure of DNA, by James Watson.The DNA double helix polymer of nucleic acids, held together by nucleotides which base pair together. In B-DNA, the most common double helical structure, the double helix is right-handed with about 10–10.5 base pairs per turn. This translates into about 20-21 nucleotides per turn. The double helix structure of DNA contains a major groove and minor groove. In B-DNA the major groove is wider than the minor groove. Given the difference in widths of the major groove and minor groove, many proteins which bind to B-DNA do so through the wider major groove.