Nucleic Acids - Farmasi Unand
... • However, other groups in the structures may also contribute to the binding of a drug to the DNA. • For example, the amino group of the sugar residue of doxorubicin forms an ionic bond with the negatively charged oxygens of the phosphate groups of the DNA chain, which effectively locks the drug in ...
... • However, other groups in the structures may also contribute to the binding of a drug to the DNA. • For example, the amino group of the sugar residue of doxorubicin forms an ionic bond with the negatively charged oxygens of the phosphate groups of the DNA chain, which effectively locks the drug in ...
Transposon stability and a role for conjugational transfer in adaptive mutability
... cells as well as self-transfer between lacI33 bacteria. We found that TetR was frequently lost in episomal Lac⫹ revertant colonies that accumulate during selection with and without an imposed requirement for conjugal transfer. This loss was found many orders of magnitude more frequently than had bee ...
... cells as well as self-transfer between lacI33 bacteria. We found that TetR was frequently lost in episomal Lac⫹ revertant colonies that accumulate during selection with and without an imposed requirement for conjugal transfer. This loss was found many orders of magnitude more frequently than had bee ...
supporting information
... 9x20 matrix in which every entry is a number quantifying the preference of a given amino acid (20 possibilities) to be placed in a given position along the bound peptide (9 possibilities). A 9x20 binding preference matrix was also constructed for this molecule. The binding matrices were used in a co ...
... 9x20 matrix in which every entry is a number quantifying the preference of a given amino acid (20 possibilities) to be placed in a given position along the bound peptide (9 possibilities). A 9x20 binding preference matrix was also constructed for this molecule. The binding matrices were used in a co ...
345 - Timstar
... common technique is a “hot start” step, in which the PCR reagents are introduced in the reaction only after the DNA is fully denatured at 94°C. In this experiment, the students will use the PCR-RFLP method to examine the presence of the polymorphism. Students will use the PCR to amplify the polymorp ...
... common technique is a “hot start” step, in which the PCR reagents are introduced in the reaction only after the DNA is fully denatured at 94°C. In this experiment, the students will use the PCR-RFLP method to examine the presence of the polymorphism. Students will use the PCR to amplify the polymorp ...
Genetic dissection of Helicobacter pylori AddAB role in homologous
... both genes, addA and addB, resulted in the same sensitivity as that of the single mutants (Fig. 2b). These results confirm that AddA and AddB act together in the repair of IR-induced DNA damage. Inactivation of the AddAB complex made the strain as sensitive as a recA mutant and its combination with ...
... both genes, addA and addB, resulted in the same sensitivity as that of the single mutants (Fig. 2b). These results confirm that AddA and AddB act together in the repair of IR-induced DNA damage. Inactivation of the AddAB complex made the strain as sensitive as a recA mutant and its combination with ...
Generation and analysis of mutated clonal scFv Jiya George
... infected people from progressing to AIDS. These protective antibodies, called anti-R7V antibodies, were able to neutralize different HIV isolates, despite their genomic variations, various cellular targets and geographic origin. Anti-R7V antibodies in the format of single chain variable fragments (s ...
... infected people from progressing to AIDS. These protective antibodies, called anti-R7V antibodies, were able to neutralize different HIV isolates, despite their genomic variations, various cellular targets and geographic origin. Anti-R7V antibodies in the format of single chain variable fragments (s ...
Specific oligonucleotide primers for detection of endoglucanase
... et al. 1999). Classical identification methods based on biochemical tests or fatty acid methyl ester profiling were laborious and hence not applicable for the purpose of a rapid screening. These taxa can be differentiated from one another by fatty acid composition analysis, restriction digest analys ...
... et al. 1999). Classical identification methods based on biochemical tests or fatty acid methyl ester profiling were laborious and hence not applicable for the purpose of a rapid screening. These taxa can be differentiated from one another by fatty acid composition analysis, restriction digest analys ...
Killing specimens
... could be difficult to collect reared specimens from these containers. Dark cages with collection jars make it easier to collect reared specimens (which are attracted to the jars as an exit). Mason jars with screen or gauze lids work for smaller situations. Be sure to include adequate space for emerg ...
... could be difficult to collect reared specimens from these containers. Dark cages with collection jars make it easier to collect reared specimens (which are attracted to the jars as an exit). Mason jars with screen or gauze lids work for smaller situations. Be sure to include adequate space for emerg ...
Isolation and characterization of a repeated sequence (RPS1) of
... and has a GC content of 40 mol%. The repeat unit contains smaller repeats of about 80-170 bp which are called REP1, REP2, REP3, REP4 and REPS; REP2 is duplicated. The small repeats are classified into two groups by their homology. One comprises REP1, REP2 and REPS, and the other REP3 and REP4. They ...
... and has a GC content of 40 mol%. The repeat unit contains smaller repeats of about 80-170 bp which are called REP1, REP2, REP3, REP4 and REPS; REP2 is duplicated. The small repeats are classified into two groups by their homology. One comprises REP1, REP2 and REPS, and the other REP3 and REP4. They ...
Nicolaus Copernicus` DNA found in Uppsala
... and responsible for the collection of old instruments at the Observatory. I have been retired since the spring of 2008. In 1973, during the celebration of the 500 year anniversary of the birth of Copernicus, Peter asked me to assist him with his Copernicus’ exhibition at the City Library in Uppsala ...
... and responsible for the collection of old instruments at the Observatory. I have been retired since the spring of 2008. In 1973, during the celebration of the 500 year anniversary of the birth of Copernicus, Peter asked me to assist him with his Copernicus’ exhibition at the City Library in Uppsala ...
Identification of markers tightly linked to tomato yellow
... outbreak, but the majority of these defensive methods are not effective. Therefore, breeding for resistance to tomato yellow leaf curl disease (TYLCD) is the most advantageous approach to controlling the damage caused by this viral disease (Castro et al., 2007; Laterrot, 1992), but no resistance has ...
... outbreak, but the majority of these defensive methods are not effective. Therefore, breeding for resistance to tomato yellow leaf curl disease (TYLCD) is the most advantageous approach to controlling the damage caused by this viral disease (Castro et al., 2007; Laterrot, 1992), but no resistance has ...
Adherin - Semantic Scholar
... captures the two sister chromatids within the ring [15] (Figure 1). Other possibilities include a ‘snap’ model in which two cohesin rings bound to the two sister chromatids loop through each other [16]. In either case, establishing cohesion requires opening of the cohesin ring. ATP hydrolysis mutati ...
... captures the two sister chromatids within the ring [15] (Figure 1). Other possibilities include a ‘snap’ model in which two cohesin rings bound to the two sister chromatids loop through each other [16]. In either case, establishing cohesion requires opening of the cohesin ring. ATP hydrolysis mutati ...
Transcription
... metabolic activities. In present day cells, DNA stores information and proteins perform catalysis, with RNA as the intermediate between DNA and protein. One can imagine a time when there was no DNA or protein, just RNA performing both functions: this is the RNA World hypothesis. • Very long ago, at ...
... metabolic activities. In present day cells, DNA stores information and proteins perform catalysis, with RNA as the intermediate between DNA and protein. One can imagine a time when there was no DNA or protein, just RNA performing both functions: this is the RNA World hypothesis. • Very long ago, at ...
FLUORESCENT PROTEIN IN Escherichia coli
... AMPs are an essential part of the innate immune system, hence their description as host defence peptides. The innate immune system evolved 2.6 billion years ago, and is present in all living organisms [Gordon et al., 2005]. It is the front-line defense against infections, and it is very rapid compar ...
... AMPs are an essential part of the innate immune system, hence their description as host defence peptides. The innate immune system evolved 2.6 billion years ago, and is present in all living organisms [Gordon et al., 2005]. It is the front-line defense against infections, and it is very rapid compar ...
Gene Section RAD52 (RAD52 homolog (S. cerevisiae)) Atlas of Genetics and Cytogenetics
... The well-studied hRAD521-212 is the N-terminal portion which forms an undecameric ringed polymer (Kagawa et al., 2002). DNA binding properties are linked to various amino acids, including, Arg-55, Tyr-65, Lys-152, Arg-153, Arg-156. Arg-55 and Lys-152 are necessarily for ssDNA binding, whereas Tyr-65 ...
... The well-studied hRAD521-212 is the N-terminal portion which forms an undecameric ringed polymer (Kagawa et al., 2002). DNA binding properties are linked to various amino acids, including, Arg-55, Tyr-65, Lys-152, Arg-153, Arg-156. Arg-55 and Lys-152 are necessarily for ssDNA binding, whereas Tyr-65 ...
arXiv:0708.2724v1 [cond-mat.other] 20 Aug 2007
... medicine is now at the forefront of scientific research. To realize this potential, however, we need a revolutionary sequencing method for the cost-effective and rapid interrogation of individual genomes. This capability is likely to be provided by a physical approach to probing DNA at the single nu ...
... medicine is now at the forefront of scientific research. To realize this potential, however, we need a revolutionary sequencing method for the cost-effective and rapid interrogation of individual genomes. This capability is likely to be provided by a physical approach to probing DNA at the single nu ...
Life 9e - Garvness
... Textbook Reference: 14.4 How Is Eukaryotic DNA Transcribed and the RNA Processed? Page: 301 Bloom’s Category: 1. Remembering 50. When eukaryotic DNA is hybridized with mRNA, the hybrid molecules contain loops of double-stranded DNA, which are a. retroviruses. b. introns. c. exons. d. transcripts. e ...
... Textbook Reference: 14.4 How Is Eukaryotic DNA Transcribed and the RNA Processed? Page: 301 Bloom’s Category: 1. Remembering 50. When eukaryotic DNA is hybridized with mRNA, the hybrid molecules contain loops of double-stranded DNA, which are a. retroviruses. b. introns. c. exons. d. transcripts. e ...
Gene silencing in mammalian cells and the spread of DNA
... Sp1 binding sites can prevent transgene (Siegfried et al., 1999) and retroviral (Hejnar et al., 2001) silencing. Additional studies provided evidence that DNA methylation patterns are not static in somatic cells, as often assumed, but instead can vary at individual CpG sites (Silva et al., 1993; Tur ...
... Sp1 binding sites can prevent transgene (Siegfried et al., 1999) and retroviral (Hejnar et al., 2001) silencing. Additional studies provided evidence that DNA methylation patterns are not static in somatic cells, as often assumed, but instead can vary at individual CpG sites (Silva et al., 1993; Tur ...
K - Romanian Biotechnological Letters
... The non-conventional yeast Kluyveromyces lactis has become an excellent alternative yeast model organism [1, 2]. Reconsidered to be a distinct species [3, 4], K. lactis is an ascomyceteous budding yeast that belongs to the endoascomycetales [1]. There are important reasons for the increased attracti ...
... The non-conventional yeast Kluyveromyces lactis has become an excellent alternative yeast model organism [1, 2]. Reconsidered to be a distinct species [3, 4], K. lactis is an ascomyceteous budding yeast that belongs to the endoascomycetales [1]. There are important reasons for the increased attracti ...
Plant centromeres: structure and control Eric J Richards and R Kelly
... begins [1]. The animal kinetochore has four plate-like layers that may have unique functions in chromosome movement. The outermost layer, known as the fibrous corona, carries molecular motors that are involved in chromosome movement [2]. Inside of the fibrous corona is an outer plate which appears t ...
... begins [1]. The animal kinetochore has four plate-like layers that may have unique functions in chromosome movement. The outermost layer, known as the fibrous corona, carries molecular motors that are involved in chromosome movement [2]. Inside of the fibrous corona is an outer plate which appears t ...
microbial genetics
... maintenance mechanisms, and drug resistance and colicin production. In bacteria, plasmid transfer occurs through transformation and conjugation. Often it is useful to transfer a nontransmissible plasmid to a specific host cell. It is possible to transfer the purified DNA as long as a genetic selecti ...
... maintenance mechanisms, and drug resistance and colicin production. In bacteria, plasmid transfer occurs through transformation and conjugation. Often it is useful to transfer a nontransmissible plasmid to a specific host cell. It is possible to transfer the purified DNA as long as a genetic selecti ...
(base) sequence of the genome might reflect biological information
... frequency of genome base sequences [20]. We have studied many genome sequences down-loaded from databases like NCBI [21], and calculated the appearance frequencies of an optional base sequence (key sequence) in a genome. Subsequently, we determined the sequence spectra of chromosome, gene and DNA fr ...
... frequency of genome base sequences [20]. We have studied many genome sequences down-loaded from databases like NCBI [21], and calculated the appearance frequencies of an optional base sequence (key sequence) in a genome. Subsequently, we determined the sequence spectra of chromosome, gene and DNA fr ...
Qβ replicase discriminates between legitimate and illegitimate
... thermodynamically more stable than are the intrastand secondary structures: If a mixture of complementary is annealed (melted and then slow cooled), they are completely converted into double helix. • Within the replicative complex, the template and the nascent strands are close to one another, which ...
... thermodynamically more stable than are the intrastand secondary structures: If a mixture of complementary is annealed (melted and then slow cooled), they are completely converted into double helix. • Within the replicative complex, the template and the nascent strands are close to one another, which ...
Extending the Implications of Myriad to Ambry â•fiThe New
... transcription. DNA naturally consists of both introns and exons. Molecular bonds separate into individual strands of DNA. These then stand alone as single strand DNA templates for forming the other strand, according to base-pairing principles. A complementary RNA strand, known as pre-RNA is formed. ...
... transcription. DNA naturally consists of both introns and exons. Molecular bonds separate into individual strands of DNA. These then stand alone as single strand DNA templates for forming the other strand, according to base-pairing principles. A complementary RNA strand, known as pre-RNA is formed. ...
Multiplex in-vitro Detection using SERS
... peaks obtained in Raman spectra have narrow spectral widths, allowing for ease of spectral separation between components. This is clearly advantageous over the broad fluorescence emission bands, which possess large spectral overlaps, making multiplexing more difficult and also providing limited stru ...
... peaks obtained in Raman spectra have narrow spectral widths, allowing for ease of spectral separation between components. This is clearly advantageous over the broad fluorescence emission bands, which possess large spectral overlaps, making multiplexing more difficult and also providing limited stru ...
Nucleic acid double helix
In molecular biology, the term double helix refers to the structure formed by double-stranded molecules of nucleic acids such as DNA. The double helical structure of a nucleic acid complex arises as a consequence of its secondary structure, and is a fundamental component in determining its tertiary structure. The term entered popular culture with the publication in 1968 of The Double Helix: A Personal Account of the Discovery of the Structure of DNA, by James Watson.The DNA double helix polymer of nucleic acids, held together by nucleotides which base pair together. In B-DNA, the most common double helical structure, the double helix is right-handed with about 10–10.5 base pairs per turn. This translates into about 20-21 nucleotides per turn. The double helix structure of DNA contains a major groove and minor groove. In B-DNA the major groove is wider than the minor groove. Given the difference in widths of the major groove and minor groove, many proteins which bind to B-DNA do so through the wider major groove.