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Recombinant DNA - Westwind Alternate School
Recombinant DNA - Westwind Alternate School

... 1. use restriction enzymes to cut the DNA of both organisms to isolate the gene(s) you want 2. place cut pieces of DNA together with DNA ligase, allow recombination 3. Have(hope) the cells take up the DNA 4. use a selection technique to determine if the DNA has recombined the way you want it to in a ...
DNA, RNA, and Protein
DNA, RNA, and Protein

... Prokaryotes have no internal membranes. They have 1 circular chromosome. Replication starts at 1 site. Two replication forks form; replication moves in opposite directions. • Replication continues until forks meet & entire chromosome is copied. ...
Biology 12 Daily Notes - Mrs. Kennedy`s Biology 12 Site!
Biology 12 Daily Notes - Mrs. Kennedy`s Biology 12 Site!

...  Property 2 - it must replicate accurately so progeny cells have the same genetic makeup  Property 3 - it must be capable of some variation (mutation) to permit evolution ...
DNA notes - Chapel Hill
DNA notes - Chapel Hill

... contains information for assembling the string of amino acids that make up a single protein.  The ribosomes required to make proteins cannot read DNA. (it’s like a foreign language)  Therefore, for DNA to code for proteins, an RNA molecule must be made. ...
“Cowboy Glossary” of Genetic Terms
“Cowboy Glossary” of Genetic Terms

... organism, encoded in DNA or RNA DNA – deoxyribose nucleic acid, present in the nucleus of the cells in all living organisms and contains all the genetic information of the organism; a molecule of DNA is formed by a double strand of millions of nucleotides joined together Base pairs – the backbone of ...
Chapter 13 – Genetic Engineering
Chapter 13 – Genetic Engineering

... – Ex – bananas, citrus fruit, strawberries, many ornamental flowers Diploid corn Tetraploid corn ...
Chapter 13 – Genetic Engineering
Chapter 13 – Genetic Engineering

... – Ex – bananas, citrus fruit, strawberries, many ornamental flowers Diploid corn Tetraploid corn ...
Chapter 13 – Genetic Engineering
Chapter 13 – Genetic Engineering

... Producing new kinds of bacteria • Can expose millions of bacteria at one time to radiation – increases chances of producing a successful mutant. – Ex – bacteria that can digest oil have been produced this way ...
molecular scissors to study gene function Marta Oliveira
molecular scissors to study gene function Marta Oliveira

... The Cas9 (CRISPR associated) enzyme is the DNA cutting enzyme – the scissors- of one particular bacteria species (Streptococcus pyogenes) which recognizes the DNA target with the help of a CRISPR RNA. This RNA is generated from the CRISPR loci matching to the target viral DNA and binds to it by base ...
DNA Structure and Function
DNA Structure and Function

... makes an error about 1000 base pairs • DNA proofreading enzymes attached to the polymerase correct the mistakes • There are usually about 3-6 permanent ...
11th Grade Science PPT
11th Grade Science PPT

S-8-2-2_Genetics and Heredity: Vocabulary Worksheet and KEY
S-8-2-2_Genetics and Heredity: Vocabulary Worksheet and KEY

... DNA. Knowing the structure of DNA would help scientists understand how DNA functions to control the development of traits in an individual. This is exactly what James Watson and Francis Crick were trying to do in the early 1950’s. Watson and Crick used data that other scientists obtained about the c ...
name
name

... BIOLOGY CP FINAL EXAM REVIEW (2013 – 2014) Know the meaning of the following terms and concepts. Cell Division (Chapter 10 & Chapter 11.4 (meiosis)) ...
BILD 10.Problem Set 3 KEY
BILD 10.Problem Set 3 KEY

Ross - Tree Improvement Program
Ross - Tree Improvement Program

... • Two copies are identical = “homozygous” • Two copies are different = “heterozygous” Homozygous parent ...
Protein Synthesis - Madison County Schools
Protein Synthesis - Madison County Schools

... Protein Synthesis What is protein?  Made in the ribosome  Made by protein synthesis  Made of amino acids (there are 20) How does it work??  You need RNA! (because DNA is too big to leave the nucleus) o Ribonucleic Acid  How’s that different from DNA? o Both are nucleic acids o RNA only has one ...
Review Questions
Review Questions

... 1. Why is transcription necessary? DNA, the recipe for making proteins, never leaves the nucleus (nucleoid region in bacteria). Yet all the protein-making machinery is located out in the cytoplasm. So how does the information get to the cytoplasm? DNA is transcribed into messenger RNA. 2. What is a ...
Unit 6 Part 2 Notes Jan 16 2012
Unit 6 Part 2 Notes Jan 16 2012

... polymorphisms in a gene sequence, the target, or immobilized DNA, is usually that of a single gene. • In this case though, the target sequence placed on any given spot within the array will differ from that of other spots in the same microarray, sometimes by only one or a few specific nucleotides. • ...
code sequence practice
code sequence practice

... Transcription – making mRNA from DNA 2. If this is your original DNA strand, what is the mRNA sequence that is synthesized? DNA Strand: C A G T G C A T T mRNA strand: 3. Now go backwards, if you are given the following mRNA strand, write the DNA strand that goes with it. mRNA strand: U C G A C C G A ...
CHAPTERS 21 AND 22
CHAPTERS 21 AND 22

... ► Second a sugar D-ribose or D-deoxyribose ► Third is a phosphate derived from phosphoric acid ...
Genetic Engineering - University of Rhode Island
Genetic Engineering - University of Rhode Island

... To carry out this “gene splicing”, first the section of the DNA containing the gene must be isolated and then cut out. The sequence is then modified as needed. Then it is spliced into a different DNA segment or into a vector (organism that doesn’t cause disease itself but rather spreads it) to trans ...
INHERITANCE
INHERITANCE

... formation of new proteins RNA uses the DNA as a template to read the code in order to produce the right protein with the correct order and number of amino acids. ...
Students Visit DNA Learning Center
Students Visit DNA Learning Center

... The size of the genome is about 165 million bases and contains and estimated 12,000 genes (by comparison, the human genome has 3,300 million bases and may have about 70,000 genes; yeast has about 5800 genes in 13.5 million base bases). ...
Banana DNA Extraction Lab
Banana DNA Extraction Lab

... The process of isolating DNA from a cell is the first step of many laboratory procedures in biotechnology. The scientist must be able to separate the DNA from the unwanted substances of the cell gently enough so that the DNA is not broken up and sheared. A “filtrate” is made of bananas and treated w ...
Genetic Engineering: How and why scientists manipulate DNA in
Genetic Engineering: How and why scientists manipulate DNA in

...  Used to treat SCID (severe combined immunodeficiency syndrome) & _______ ___________  Trials for sickle-cell and hemophilia treatments are occurring now. ...
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Nucleic acid double helix



In molecular biology, the term double helix refers to the structure formed by double-stranded molecules of nucleic acids such as DNA. The double helical structure of a nucleic acid complex arises as a consequence of its secondary structure, and is a fundamental component in determining its tertiary structure. The term entered popular culture with the publication in 1968 of The Double Helix: A Personal Account of the Discovery of the Structure of DNA, by James Watson.The DNA double helix polymer of nucleic acids, held together by nucleotides which base pair together. In B-DNA, the most common double helical structure, the double helix is right-handed with about 10–10.5 base pairs per turn. This translates into about 20-21 nucleotides per turn. The double helix structure of DNA contains a major groove and minor groove. In B-DNA the major groove is wider than the minor groove. Given the difference in widths of the major groove and minor groove, many proteins which bind to B-DNA do so through the wider major groove.
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