B.2 Specific Aims. The term `epigenetics` literally means `above the
... aerobic exercise was associated with decreased methylation of the tumor suppressor gene L3MBTL.[17] Our group demonstrated that healthy adults who increased physical activity over a 12-month exercise program had decreased DNA methylation across a panel of markers associated with breast cancer.[7] Mo ...
... aerobic exercise was associated with decreased methylation of the tumor suppressor gene L3MBTL.[17] Our group demonstrated that healthy adults who increased physical activity over a 12-month exercise program had decreased DNA methylation across a panel of markers associated with breast cancer.[7] Mo ...
Significance of bacterial identification by molecular
... amplifies enough specific copies to be able to carry out any number of other molecular biology applications e.g. size determination (in bases) and its nucleotide sequence. The particular stretch of DNA to be amplified, called the target sequence, is identified by a specific pair of DNA primers, olig ...
... amplifies enough specific copies to be able to carry out any number of other molecular biology applications e.g. size determination (in bases) and its nucleotide sequence. The particular stretch of DNA to be amplified, called the target sequence, is identified by a specific pair of DNA primers, olig ...
Overexpression of the catalytic subunit of DNA polymerase results in
... and a precise program of organelle biogenesis is needed during development. Recently, mitochondrial transcription factor A (Tfam) has been shown to be essential for mitochondrial biogenesis. Disruption of the Tfam gene in mouse produces a severe depletion of mtDNA, thereby abolishing oxidative phosp ...
... and a precise program of organelle biogenesis is needed during development. Recently, mitochondrial transcription factor A (Tfam) has been shown to be essential for mitochondrial biogenesis. Disruption of the Tfam gene in mouse produces a severe depletion of mtDNA, thereby abolishing oxidative phosp ...
Document
... LSD1 coordinats histone methylation and DNA methylation Methylated Dnmt1 is metabolically unstable LSD1, by acting directly on both histone H3 and Dnmt1, causes H3K4 demethylation & ↑ Dnmt1 & DNA methylation, Results in chromatin condensation & gene silencing ...
... LSD1 coordinats histone methylation and DNA methylation Methylated Dnmt1 is metabolically unstable LSD1, by acting directly on both histone H3 and Dnmt1, causes H3K4 demethylation & ↑ Dnmt1 & DNA methylation, Results in chromatin condensation & gene silencing ...
Nucleolar caspase-2: Protecting us from DNA damage
... pretreatment of cells with the DNA-damaging agent camptothecin inducing the interaction. However, apoptosis induced by the non–DNA-damaging agent actinomycin D (a microtubule inhibitor) did not induce the interaction. The full-length PIDD polypeptide is constitutively autoprocessed into three fragme ...
... pretreatment of cells with the DNA-damaging agent camptothecin inducing the interaction. However, apoptosis induced by the non–DNA-damaging agent actinomycin D (a microtubule inhibitor) did not induce the interaction. The full-length PIDD polypeptide is constitutively autoprocessed into three fragme ...
Comparison of three molecular methods for typing Aeromonas
... For instance, an identical ISR-RFLP type was obtained for isolates 203, 210 and 137 (Figure 1), while they showed specific patterns with the other two methods (Figures 2, 3). This may be considered a shortcoming of the ISR-RFLP method and additionally, as already commented, it is more complex to perf ...
... For instance, an identical ISR-RFLP type was obtained for isolates 203, 210 and 137 (Figure 1), while they showed specific patterns with the other two methods (Figures 2, 3). This may be considered a shortcoming of the ISR-RFLP method and additionally, as already commented, it is more complex to perf ...
1 X chromosome crossover formation and genome stability in
... The germ line efficiently combats numerous genotoxic insults to ensure the high fidelity propagation of unaltered genomic information across generations. Yet, germ cells in most metazoans also intentionally create double-strand breaks (DSBs) to promote DNA exchange between parental chromosomes, a pr ...
... The germ line efficiently combats numerous genotoxic insults to ensure the high fidelity propagation of unaltered genomic information across generations. Yet, germ cells in most metazoans also intentionally create double-strand breaks (DSBs) to promote DNA exchange between parental chromosomes, a pr ...
pdf
... Mazard et al. (2004) from surface waters of the Arabian Sea, as well as with sequences from surface waters in many other locations, including the North Pacific, North Atlantic, and South China Sea. Although several clones (32 from each depth) of the PCR products obtained with the nested nifH primers ...
... Mazard et al. (2004) from surface waters of the Arabian Sea, as well as with sequences from surface waters in many other locations, including the North Pacific, North Atlantic, and South China Sea. Although several clones (32 from each depth) of the PCR products obtained with the nested nifH primers ...
Lampbrush Chromosomes of the Chicken
... small distinct set of loops. In general, loops on this chromosome seems less extended than those on other chromosomes in the same spread. Chromosome length and loop size are a function of the stage in the progressive formation and retraction/compaction process as diplotene progresses and the oocyte ...
... small distinct set of loops. In general, loops on this chromosome seems less extended than those on other chromosomes in the same spread. Chromosome length and loop size are a function of the stage in the progressive formation and retraction/compaction process as diplotene progresses and the oocyte ...
GCAT-SEEK Workshop - Prokaryotic Genomics Module – Jeff
... in the sequences can be used to assemble the reads into larger contiguous sequences or “contigs” There are many algorithms for assembly, but most of the free ones run in a linux environment. The limitations in experience with and access to linux for most students and faculty teaching undergraduates ...
... in the sequences can be used to assemble the reads into larger contiguous sequences or “contigs” There are many algorithms for assembly, but most of the free ones run in a linux environment. The limitations in experience with and access to linux for most students and faculty teaching undergraduates ...
Genetic identification of eleven aquatic bacteria using the 16S rDNA
... determination of the relative proportion of guanine and cytosine, however, this method does not rely on the linear arrangement of the nucleotides, and therefore, its accuracy is low. DNA and RNA homology experiments identify bacteria by hybridization of DNA or RNA molecules between species, but the ...
... determination of the relative proportion of guanine and cytosine, however, this method does not rely on the linear arrangement of the nucleotides, and therefore, its accuracy is low. DNA and RNA homology experiments identify bacteria by hybridization of DNA or RNA molecules between species, but the ...
ACLS CH05 - CTCE Moodle
... DNA is isolated from an organism that contains the desired gene. It is purified and fragmented, and the segments are inserted into plasmids, forming recombinants that are placed into bacterial host cells. Bacteria containing the plasmids grow on an agar medium, producing a colony of cells contai ...
... DNA is isolated from an organism that contains the desired gene. It is purified and fragmented, and the segments are inserted into plasmids, forming recombinants that are placed into bacterial host cells. Bacteria containing the plasmids grow on an agar medium, producing a colony of cells contai ...
File
... a. A gene from an organism can theoretically be expressed by any other organism. b. All organisms have experienced convergent evolution. c. DNA was the first genetic material. d. The same codons in different organisms translate into the different amino acids. e. Different organisms have different nu ...
... a. A gene from an organism can theoretically be expressed by any other organism. b. All organisms have experienced convergent evolution. c. DNA was the first genetic material. d. The same codons in different organisms translate into the different amino acids. e. Different organisms have different nu ...
Identification of Human Polymorphisms in the Phenylthio
... genotypes observed was thus 5. The above results represent pooled numbers for two different study groups (families and unrelated individuals). The AVI/AVI nontaster phenotype was even more definitive in the unrelated study group, e.g., 21 out of 21 nontasters (Kim et al., 2003). One question to ex ...
... genotypes observed was thus 5. The above results represent pooled numbers for two different study groups (families and unrelated individuals). The AVI/AVI nontaster phenotype was even more definitive in the unrelated study group, e.g., 21 out of 21 nontasters (Kim et al., 2003). One question to ex ...
Part III: Laboratory – Electrophoresis
... The laboratory is organized by part. Each part has notes for the instructor (when necessary), preparation instructions, and the experimental protocol for the lab. A separate Results and Discussion is also provided with some additional suggested analysis. The lab is set-up to have students work in pa ...
... The laboratory is organized by part. Each part has notes for the instructor (when necessary), preparation instructions, and the experimental protocol for the lab. A separate Results and Discussion is also provided with some additional suggested analysis. The lab is set-up to have students work in pa ...
Phylogenetic Affinity of Mitochondria of Euglena
... Hoechst-CsCl ultracentrifugation was also used for purification of mitochondrial DNA from total cellular DNA (Simpson 1979). Estimation of Genomic Complexity by Quantitative Hybridization. The same amount of purified total mitochondrial DNA and a randomly cloned fragment were serially diluted and sp ...
... Hoechst-CsCl ultracentrifugation was also used for purification of mitochondrial DNA from total cellular DNA (Simpson 1979). Estimation of Genomic Complexity by Quantitative Hybridization. The same amount of purified total mitochondrial DNA and a randomly cloned fragment were serially diluted and sp ...
Transition Bias
... G/U pair, although not as strong as A/U or C/G pair, generally does not disrupt RNA secondary structure (and occurs frequently in RNA secondary ...
... G/U pair, although not as strong as A/U or C/G pair, generally does not disrupt RNA secondary structure (and occurs frequently in RNA secondary ...
Towards Programmable Molecular Machines
... proposal extends the walker proposed by Yin et al. [15] and provides a method for controlling the movement of the walker and for the walker to take input from (and write output to) the substrate. Some of the techniques we use are similar to the ones described in [13]. In our proposal, the substrate ...
... proposal extends the walker proposed by Yin et al. [15] and provides a method for controlling the movement of the walker and for the walker to take input from (and write output to) the substrate. Some of the techniques we use are similar to the ones described in [13]. In our proposal, the substrate ...
Evidence for Variable Selective Pressures at a
... human mitochondrial DNA (mtDNA) control region. Most studies targeting this noncoding segment usually focus on its primary sequence information disregarding other informative levels such as secondary or tertiary DNA conformations. In this work, we combined the most recent developments in DNA folding ...
... human mitochondrial DNA (mtDNA) control region. Most studies targeting this noncoding segment usually focus on its primary sequence information disregarding other informative levels such as secondary or tertiary DNA conformations. In this work, we combined the most recent developments in DNA folding ...
Full wwPDB X-ray Structure Validation Report i
... fraction of residues that contain outliers for >=3, 2, 1 and 0 types of geometric quality criteria. A grey segment represents the fraction of residues that are not modelled. The numeric value for each fraction is indicated below the corresponding segment, with a dot representing fractions <=5% The u ...
... fraction of residues that contain outliers for >=3, 2, 1 and 0 types of geometric quality criteria. A grey segment represents the fraction of residues that are not modelled. The numeric value for each fraction is indicated below the corresponding segment, with a dot representing fractions <=5% The u ...
DNA breathing dynamics distinguish binding from nonbinding
... from nonbinding sites in the human PLG promoter YY1 knockdown in HeLa cells coincides with the accumulation of plasminogen (PLG) mRNA (not shown). The gene product regulates a wide variety of biologic responses directly related to the development of cardiovascular disease including atherosclerosis a ...
... from nonbinding sites in the human PLG promoter YY1 knockdown in HeLa cells coincides with the accumulation of plasminogen (PLG) mRNA (not shown). The gene product regulates a wide variety of biologic responses directly related to the development of cardiovascular disease including atherosclerosis a ...
Separation of DNA Restriction Fragments by Ion
... (B) They have a high potential for nucleic acid separation as indicated by our preliminary investigation (20). (C) They have suitable chemical and physical properties (19). As opposed to the silica-based supports, Mono P and Mono Q are not alkali labile. Furthermore, the ion-exchange groups are cova ...
... (B) They have a high potential for nucleic acid separation as indicated by our preliminary investigation (20). (C) They have suitable chemical and physical properties (19). As opposed to the silica-based supports, Mono P and Mono Q are not alkali labile. Furthermore, the ion-exchange groups are cova ...
DNA Mismatch Repair and Synonymous Codon Evolution in
... vary between genes is by differences in the efficiency of mismatch repair (Filipski 1988 ) . For instance, if some base mismatches are more efficiently repaired than others, or if certain mismatches tend to be repaired in a particular direction, then the mutation pattern will be affected, and effici ...
... vary between genes is by differences in the efficiency of mismatch repair (Filipski 1988 ) . For instance, if some base mismatches are more efficiently repaired than others, or if certain mismatches tend to be repaired in a particular direction, then the mutation pattern will be affected, and effici ...
Nucleic acid double helix
In molecular biology, the term double helix refers to the structure formed by double-stranded molecules of nucleic acids such as DNA. The double helical structure of a nucleic acid complex arises as a consequence of its secondary structure, and is a fundamental component in determining its tertiary structure. The term entered popular culture with the publication in 1968 of The Double Helix: A Personal Account of the Discovery of the Structure of DNA, by James Watson.The DNA double helix polymer of nucleic acids, held together by nucleotides which base pair together. In B-DNA, the most common double helical structure, the double helix is right-handed with about 10–10.5 base pairs per turn. This translates into about 20-21 nucleotides per turn. The double helix structure of DNA contains a major groove and minor groove. In B-DNA the major groove is wider than the minor groove. Given the difference in widths of the major groove and minor groove, many proteins which bind to B-DNA do so through the wider major groove.