16S rRNA characterization of Bacillus strain and its
... Testing the KatA gene responsible for hydrogen peroxide tolerance in the two samples showed higher band intensity in the frozen sample when compared to the subcultured sample. This indicates that the hydrogen peroxide tolerance trait is extrachromosomal and has been lost during successive cell divis ...
... Testing the KatA gene responsible for hydrogen peroxide tolerance in the two samples showed higher band intensity in the frozen sample when compared to the subcultured sample. This indicates that the hydrogen peroxide tolerance trait is extrachromosomal and has been lost during successive cell divis ...
Cot-1 banding of human chromosomes using fluorescence
... The scanning analysis of the fluorescence intensity is useful in quantifying a banding pattern and the resolution is sufficient for locating a hybridization signal on banded chromosomes. This could be useful for more precise analysis of chromosome aberrations and gene localization. The scanning anal ...
... The scanning analysis of the fluorescence intensity is useful in quantifying a banding pattern and the resolution is sufficient for locating a hybridization signal on banded chromosomes. This could be useful for more precise analysis of chromosome aberrations and gene localization. The scanning anal ...
DNA methylation controls histone H3 lysine 9 methylation
... Briggs et al., 2001) (recently reviewed in Rice and Allis, 2001; Lachner and Jenuwein, 2002; Richards and Elgin, 2002). The interactions between DNA methylation, histone modi®cations and chromatin structure have mainly been studied at the molecular level for speci®c DNA sequences. Integrated genetic ...
... Briggs et al., 2001) (recently reviewed in Rice and Allis, 2001; Lachner and Jenuwein, 2002; Richards and Elgin, 2002). The interactions between DNA methylation, histone modi®cations and chromatin structure have mainly been studied at the molecular level for speci®c DNA sequences. Integrated genetic ...
Structural variations in the human genome
... world, perhaps even in the entire universe. Its massive amount of base pairs consisting of a varying number of genes (per organism) contains hereditary information that is used in the development and functioning of an entire organism. In fact, it is hard to imagine life or living without DNA being i ...
... world, perhaps even in the entire universe. Its massive amount of base pairs consisting of a varying number of genes (per organism) contains hereditary information that is used in the development and functioning of an entire organism. In fact, it is hard to imagine life or living without DNA being i ...
Graph-based Methods for the Design of DNA - TUBdok
... of hybridization between pairs of DNA single strands, are considered as the most reliable criterion to compose such sequences. The purpose of this thesis is to provide a contribution to the field of finding reliable DNA sequences for the encoding of entities in mathematical problems. The developed m ...
... of hybridization between pairs of DNA single strands, are considered as the most reliable criterion to compose such sequences. The purpose of this thesis is to provide a contribution to the field of finding reliable DNA sequences for the encoding of entities in mathematical problems. The developed m ...
Synapsis-Mediated Fusion of Free DNA Ends Forms Inverted Dimer Plasmids in Yeast.
... the plasmid do not share homology with the yeast genome, circular inverted (head-to-head) dimer plasmids are theprincipal product of repair. By measurements of the DNA concentration dependence of transformation with alinearized plasmid, and by transformation with mixtures of genetically marked plasm ...
... the plasmid do not share homology with the yeast genome, circular inverted (head-to-head) dimer plasmids are theprincipal product of repair. By measurements of the DNA concentration dependence of transformation with alinearized plasmid, and by transformation with mixtures of genetically marked plasm ...
DNA Duplication Associated with Charcot-Marie-Tooth Disease Type 1A. Lupski, et al., 1991 Cell, Vol. 66, 219-232, July 26, 1991,
... and covers a distance of 9.9 CM. The five families contain approximately 108 meioses, which for the LEW301-1517 interval should contain 9.7 f 3.1 recombinants. The observed number of recombinants (5) is well within expectations (x2 = 2.30, 1 degree of freedom, P > 0.10). These recombinants suggest t ...
... and covers a distance of 9.9 CM. The five families contain approximately 108 meioses, which for the LEW301-1517 interval should contain 9.7 f 3.1 recombinants. The observed number of recombinants (5) is well within expectations (x2 = 2.30, 1 degree of freedom, P > 0.10). These recombinants suggest t ...
Damage Control: The Pleiotropy of DNA Repair Genes
... cells. This result has been confirmed and extended to cells in the eye imaginal disc (M. Brodsky and G. M. Rubin, personal communication). Thus the function of the MEI-41 protein may not be in the repair of damage per se, but in triggering a DNA damage-dependent cellcycle checkpoint. Activation of t ...
... cells. This result has been confirmed and extended to cells in the eye imaginal disc (M. Brodsky and G. M. Rubin, personal communication). Thus the function of the MEI-41 protein may not be in the repair of damage per se, but in triggering a DNA damage-dependent cellcycle checkpoint. Activation of t ...
Add Health Biomarker - Carolina Population Center
... The urine specimen would be tested for two curable STIs—chlamydia and gonorrhea. The urine samples would also be tested for other STIs but, because those tests have not yet been approved by the FDA as reliable using urine specimens, they would only be used for exploratory purposes and their results ...
... The urine specimen would be tested for two curable STIs—chlamydia and gonorrhea. The urine samples would also be tested for other STIs but, because those tests have not yet been approved by the FDA as reliable using urine specimens, they would only be used for exploratory purposes and their results ...
Clinical and Molecular Aspects of Diseases of Mitochondrial DNA
... and the preponderance of oocyte mtDNAs ensure strict maternal inheritance of mtDNA. Human mtDNA is compact and economical, managing to compress 37 genes into fewer than 20 kilobases of DNA. There is, however, one substantial non-coding region (NCR), of ~900 bp in mammals (Fig. 1). Within the NCR, ma ...
... and the preponderance of oocyte mtDNAs ensure strict maternal inheritance of mtDNA. Human mtDNA is compact and economical, managing to compress 37 genes into fewer than 20 kilobases of DNA. There is, however, one substantial non-coding region (NCR), of ~900 bp in mammals (Fig. 1). Within the NCR, ma ...
State v. Unsworth
... Pharmaceuticals, Inc., 509 U.S. 579, 113 S.Ct. 2786, 125 L.Ed.2d 469 (1993)) to exclude from evidence the results of genetic testing, arguing that the testing procedures were ...
... Pharmaceuticals, Inc., 509 U.S. 579, 113 S.Ct. 2786, 125 L.Ed.2d 469 (1993)) to exclude from evidence the results of genetic testing, arguing that the testing procedures were ...
i. Genetics
... The bases always pair up in the same way Adenine forms a bond with Thymine Adenine ...
... The bases always pair up in the same way Adenine forms a bond with Thymine Adenine ...
statistical issues in the analysis of microbial communities in soil
... variable sequences. This structure allows for effective discrimination between different microorganisms (Pace et al., 1986). For example, some of the more highly conserved nucleotide sequences common to all bacteria can be used to distinguish bacteria from other organisms, while some of the more var ...
... variable sequences. This structure allows for effective discrimination between different microorganisms (Pace et al., 1986). For example, some of the more highly conserved nucleotide sequences common to all bacteria can be used to distinguish bacteria from other organisms, while some of the more var ...
Non-Type 1 Cystinuria Marker DNA Test for Mastiff
... Non-‐Type 1 Cystinuria Marker DNA Test for Mastiff-‐Type Breeds The Science and Clinical Implications Behind the Test There are at least two types of cystinuria in dogs. Type-‐I cystinuria, well docume ...
... Non-‐Type 1 Cystinuria Marker DNA Test for Mastiff-‐Type Breeds The Science and Clinical Implications Behind the Test There are at least two types of cystinuria in dogs. Type-‐I cystinuria, well docume ...
What are SNPs
... SNP Validation refers to genetic validation, the process of ensuring that the SNP is not due to sequencing error and that it is not extremely rear. This should not be confused with assay, target or regulatory validation. Confirmation of SNPs found in Discovery Larger numbers of individual samples to ...
... SNP Validation refers to genetic validation, the process of ensuring that the SNP is not due to sequencing error and that it is not extremely rear. This should not be confused with assay, target or regulatory validation. Confirmation of SNPs found in Discovery Larger numbers of individual samples to ...
Laboratory manual for the diagnosis of whooping cough caused by... pertussis/ Bordetella parapertussis
... Even in high-coverage countries, pertussis disease continues to cause severe illness and death among neonates and infants too young to have completed the primary vaccination series. Active primary immunization against B. pertussis infection is recommended with three doses of a vaccine consisting of ...
... Even in high-coverage countries, pertussis disease continues to cause severe illness and death among neonates and infants too young to have completed the primary vaccination series. Active primary immunization against B. pertussis infection is recommended with three doses of a vaccine consisting of ...
Lab (3) Report: Population Genetics
... was finally added, along with the restriction enzyme HaeIII, and the mixture was spun. The TA also stained our agarose gel with ethidium bromide which slips between stacked base pair of the DNA so that florescence is produced when UV light is shown on the gel. We then implement PCR through denaturin ...
... was finally added, along with the restriction enzyme HaeIII, and the mixture was spun. The TA also stained our agarose gel with ethidium bromide which slips between stacked base pair of the DNA so that florescence is produced when UV light is shown on the gel. We then implement PCR through denaturin ...
Biotechnology Explorer™ Ligation and Transformation - Bio-Rad
... vectors frequently contain reporter genes, which distinguish them from cells that do not have inserts. Two common reporter genes are beta-galactosidase (b-gal) and green fluorescent protein (GFP) Some newer plasmid vectors use positive selection, in which the inserted DNA interrupts a gene that woul ...
... vectors frequently contain reporter genes, which distinguish them from cells that do not have inserts. Two common reporter genes are beta-galactosidase (b-gal) and green fluorescent protein (GFP) Some newer plasmid vectors use positive selection, in which the inserted DNA interrupts a gene that woul ...
Ab Initio Modeling of Biological Systems - Psi-k
... However, there are many areas in which the use of effective potentials may be not appropriate, and more sophisticated and accurate approaches are required. An alternative is offered by ab initio molecular dynamics (AIMD). AIMD in the Born-Oppheneimer approximation can be efficiently performed using ...
... However, there are many areas in which the use of effective potentials may be not appropriate, and more sophisticated and accurate approaches are required. An alternative is offered by ab initio molecular dynamics (AIMD). AIMD in the Born-Oppheneimer approximation can be efficiently performed using ...
Chapter 18: Gene Mutation and DNA Repair
... Mutations can be caused in one of two ways. Spontaneous mutations are changes in DNA structure that result from natural biological or chemical processes, whereas induced mutations are caused by environmental agents (Refer to Table 18.4). This section examines spontaneous mutations, and the following ...
... Mutations can be caused in one of two ways. Spontaneous mutations are changes in DNA structure that result from natural biological or chemical processes, whereas induced mutations are caused by environmental agents (Refer to Table 18.4). This section examines spontaneous mutations, and the following ...
Computer-Aided DNA Base Calling from Forward and Reverse
... representing the correctness probability: the higher the quality the lower the error probability. Since our approach generates a synthetic electropherogram to be processed by a base caller, in the result section we also compare quality distributions to show the effectiveness of the proposed techniqu ...
... representing the correctness probability: the higher the quality the lower the error probability. Since our approach generates a synthetic electropherogram to be processed by a base caller, in the result section we also compare quality distributions to show the effectiveness of the proposed techniqu ...
Original Article:
... septicaemia. Bacteraemic infections often develop from the resident S. aureus flora located in the anterior nares of the patient. The understanding of the biological nature of S. aureus colonization is still limited, and most studies of the nasal carriage presume that individuals are colonized by a ...
... septicaemia. Bacteraemic infections often develop from the resident S. aureus flora located in the anterior nares of the patient. The understanding of the biological nature of S. aureus colonization is still limited, and most studies of the nasal carriage presume that individuals are colonized by a ...
Aminoacyl-tRNA Synthetase Production for Unnatural Amino Acid
... innovative tool for accomplishing these aims is cell-free protein synthesis (CFPS). This technique, rather than using living cells to make protein, simply extracts the cells’ natural protein-making machinery and then uses it to produce protein in vitro. Because living cells are no longer involved, s ...
... innovative tool for accomplishing these aims is cell-free protein synthesis (CFPS). This technique, rather than using living cells to make protein, simply extracts the cells’ natural protein-making machinery and then uses it to produce protein in vitro. Because living cells are no longer involved, s ...
Translation
... Some of tRNAs require, for binding to mRNA during translation, precise complementary pairing only on the first two positions of codon. ...
... Some of tRNAs require, for binding to mRNA during translation, precise complementary pairing only on the first two positions of codon. ...
Translation
... Some of tRNAs require, for binding to mRNA during translation, precise complementary pairing only on the first two positions of codon. ...
... Some of tRNAs require, for binding to mRNA during translation, precise complementary pairing only on the first two positions of codon. ...
Nucleic acid double helix
In molecular biology, the term double helix refers to the structure formed by double-stranded molecules of nucleic acids such as DNA. The double helical structure of a nucleic acid complex arises as a consequence of its secondary structure, and is a fundamental component in determining its tertiary structure. The term entered popular culture with the publication in 1968 of The Double Helix: A Personal Account of the Discovery of the Structure of DNA, by James Watson.The DNA double helix polymer of nucleic acids, held together by nucleotides which base pair together. In B-DNA, the most common double helical structure, the double helix is right-handed with about 10–10.5 base pairs per turn. This translates into about 20-21 nucleotides per turn. The double helix structure of DNA contains a major groove and minor groove. In B-DNA the major groove is wider than the minor groove. Given the difference in widths of the major groove and minor groove, many proteins which bind to B-DNA do so through the wider major groove.