• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
8.3 - Pattern in Nature
8.3 - Pattern in Nature

... Cell Wall: Also found only in plant cells, this organelle surrounds the whole cell outside the cell membrane. It provides strength, protection, support and shape to the plant. Cell walls are non-living - they are made of a network of cellulose microfibrils cemented together in pectin and other subst ...
Name
Name

... experienced this elevated body temperature, called a fever. The increased body temperature is advantageous because many pathogens can survive only within a narrow temperature range. An elevated temperature slows down or stops the growth of such pathogens. The higher body temperature also increases t ...
a) Compaction
a) Compaction

... 4. Excretory inclusions are products of life activity of the cells, which should be removed from the cell. ...
Cnidaria and Ctenophores
Cnidaria and Ctenophores

... efficient devices for capturing prey and extremely effective deterrents to predators. Each contains a coiled, tubular thread, which may bear barbs and which is often poisoned. A nematocyst discharges when a prey species or predator comes into contact with it, driving its threads with barb and poison ...
8.2 Cells and Energy
8.2 Cells and Energy

... Until the players are trained, they are reserves. They have the potential to do a lot of different things. Once they are trained, they become specialized in a position. The players might be defenders or forwards. They might play goalie or mid field. Similarly, embryonic stem cells are generic cells ...
1. Cells and Organelles
1. Cells and Organelles

... are known as liposomes) or bilayers in which their hydrophobic tails pack together to avoid water  Phospholipids are amphipathic- they have a polar/hydrophilic phosphate head with hydrophobic aliphatic fatty acid chains which may be saturated or unsaturated  Unsaturated hydrocarbon chains have cis ...
Unit 2 Multicellular Organisms Mr Gravell
Unit 2 Multicellular Organisms Mr Gravell

... As well as the nervous system, parts of the body can communicate through hormones. Hormones are chemical messengers produced by endocrine glands. The endocrine glands release their hormones directly into the blood as it flows through the gland. Hormones travel around the body in the blood but they h ...
Spring Final Review Guide
Spring Final Review Guide

... Electron transport chain-oxygen is used to pick up electrons and forms H2O Source of energy on surface of planet Food chain, food web: producer, consumers, detritivores, decomposers, autotroph, heterotroph, trohic levels, energy and food pyramids community, population energy flow in heterotrophic ce ...
LECTURE OUTLINE
LECTURE OUTLINE

... The tissues of the human body can be categorized into four major types: epithelial tissue, connective tissue, muscular tissue, and nervous tissue. Cancers are classified according to the type of tissue from which they arise. Epithelial Tissue Epithelial tissue consists of tightly packed cells that f ...
General facts about pregnancy
General facts about pregnancy

... Cell proliferation: nerve cells begin to form in the neural tube at a rate of 250,000 per minute for the entire pregnancy. 30,000 synapses are formed every second. Baby is born with 100 billion neurons. Cell migration: begins during 7th week; neurons begin their destination in the developing brain. ...
histology / tissue level of organization
histology / tissue level of organization

... • Tissues are groups of similar cells and extracellular products that carry out a common function. ...
Chapter 20: Tissues & Organ Systems The Importance of Homeostasis
Chapter 20: Tissues & Organ Systems The Importance of Homeostasis

... Tissue that transmits electrochemical signals. ...
Open Circulatory System
Open Circulatory System

... air or water, we don’t need a circulatory system Each cell can get what it wants and get rid of what it needs on its own. ...
Levels of Organization
Levels of Organization

... exploration mission searching for life on other planets. The commander of the ship has sent you and three of your crewmates to the surface of a newly discovered planet. Your mission is to solve a mystery. You must find out what gas the other life forms exhale. Your only clue is a CD video clip of on ...


... Q.8. (a) Differentiate between epidermal and cork cells. (b) Why are they called protective tissues ? Ans: (a) Epidermis forms one cell thick outermost layer of the organs of plants, such as, leaves, flowers, stems and roots. This layer of cells is covered from the outside by the cuticle. Cork is ma ...
Global effects of plant growth
Global effects of plant growth

... creates gradient  cotransport of other ...
www.sasd.us
www.sasd.us

... Lymphocytes that mature in the thymus above the heart are called T cells, and those that mature in bone marrow are called B cells. These are the cells involved in the ...
Tissues and Membranes
Tissues and Membranes

... Consists of intercalated discs Found making up only the heart Contract and relax in a non-voluntary pulsating manner ...
lecture 4
lecture 4

... • Fluorescein labeled Ab’s are layered over it • Slide is washed to remove unattached Ab’s • Examined under UV light in an fluorescent microscope • The site where the Ab attaches to its specific Ag will show apple green fluorescence • Use: Direct detection of Pathogens or their Ag’s in tissues or in ...
Slide 1
Slide 1

... Chemical signaling molecules that diffuse from axon of one neuron to dendrite of next, over narrow cleft between neurons known as synapse. ...
B - Images
B - Images

... a point of attachment to the trunk than another body part Distal- farther down from the point of attachment of a limb. ...
B2 Revision MATs - Hodge Hill College
B2 Revision MATs - Hodge Hill College

... Keywords: high concentration, low concentration, concentration gradient, water movement ...
Unit 1 Cell Biology Topic 3: Producing new cells
Unit 1 Cell Biology Topic 3: Producing new cells

... I will know I am successful if I can: 1. State that each of the two cells produced by mitosis maintains a diploid chromosome complement and explain why this is important. 2. Describe the stages of mitosis 3. Observe prepared root tip cell slides ...
Homeostasis and Interacting Behavior What is hom
Homeostasis and Interacting Behavior What is hom

... • The nervous system carries electrical messages throughout the body. • Divided into two main systems: • The central nervous system (CNS) • Brain and spinal cord • The peripheral nervous system (PNS) • All other nerves • Neuron Structure • Cell body - the nucleus and other cell organelles. • Dendrit ...
cells, cellular respiration, and heredity.
cells, cellular respiration, and heredity.

... Previous/Future knowledge: In 5th grade (5-2.1), students recalled that the smallest unit of life was the cell and identified its major structures (including cell membrane, cytoplasm, nucleus, and vacuole). In 6th grade (6-2.1), students summarized the characteristics that all organisms share (inclu ...
< 1 ... 20 21 22 23 24 25 26 27 28 ... 80 >

Neuronal lineage marker



A Neuronal lineage marker is an endogenous tag that is expressed in different cells along neurogenesis and differentiated cells as neurons. It allows detection and identification of cells by using different techniques. A neuronal lineage marker can be either DNA, mRNA or RNA expressed in a cell of interest. It can also be a protein tag, as a partial protein, a protein or a epitope that discriminates between different cell types or different states of a common cell. An ideal marker is specific to a given cell type in normal conditions and/or during injury. Cell markers are very valuable tools for examining the function of cells in normal conditions as well as during disease. The discovery of various proteins specific to certain cells led to the production of cell-type-specific antibodies that have been used to identify cells.The techniques used for its detection can be immunohistochemistry, immunocytochemistry, methods that utilize transcriptional modulators and site-specific recombinases to label specific neuronal population, in situ hybridization or fluorescence in situ hybridization (FISH). A neuronal lineage marker can be a neuronal antigen that is recognized by an autoantibody for example Hu, which is highly restricted to neuronal nuclei. By immunohistochemistry, anti-Hu stains the nuclei of neurons. To localize mRNA in brain tissue, one can use a fragment of DNA or RNA as a neuronal lineage marker, a hybridization probe that detects the presence of nucleotide sequences that are complementary to the sequence in the probe. This technique is known as in situ hybridization. Its application have been carried out in all different tissues, but particularly useful in neuroscience. Using this technique, it is possible to locate gene expression to specific cell types in specific regions and observe how changes in this distribution occur throughout the development and correlate with the behavioral manipulations.Although immunohistochemistry is the staple methodology for identifying neuronal cell types, since it is relatively low in cost and a wide range of immunohistochemical markers are available to help distinguish the phenotype of cells in the brain, sometimes it is time-consuming to produce a good antibody. Therefore, one of the most convenient methods for the rapid assessment of the expression of a cloned ion channel could be in situ hybridization histochemistry.After cells are isolated from tissue or differentiated from pluripotent precursors, the resulting population needs to be characterized to confirm whether the target population has been obtained. Depending on the goal of a particular study, one can use neural stem cells markers, neural progenitor cell markers, neuron markers or PNS neuronal markers.
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report