Comparative study of pathogenesis-related protein - NOPR
... (Table 3). Since these are the most variable regions among comparisons of homologous proteins, such differences are often found in acceptable structural models25. Molecular modeling revealed that PR5 proteins of Zingeberaceae species possessed the same 3-D folds and core structures that are conserve ...
... (Table 3). Since these are the most variable regions among comparisons of homologous proteins, such differences are often found in acceptable structural models25. Molecular modeling revealed that PR5 proteins of Zingeberaceae species possessed the same 3-D folds and core structures that are conserve ...
The Euglena gracilis chloroplast rpoB gene
... with chloroform-isoamyl alcohol (24:1), and collected by ethanol precipitation. Before the RNA was utilized for cDNA synthesis, the DNA was digested with RQ1 RNase-free DNase (Promega Biotechnology, Madison,WI), followed by phenol and chloroform extractions and ethanol precipitation as previously de ...
... with chloroform-isoamyl alcohol (24:1), and collected by ethanol precipitation. Before the RNA was utilized for cDNA synthesis, the DNA was digested with RQ1 RNase-free DNase (Promega Biotechnology, Madison,WI), followed by phenol and chloroform extractions and ethanol precipitation as previously de ...
v11a32-hawse pgmkr
... between matched lens epithelial and cortical fiber cells. We demonstrate that 2,463 genes exhibit altered expression levels of 2 fold or greater between lens epithelial cells relative to cortical fibers. Of these, 222 genes are upregulated and 135 genes are downregulated in the lens epithelium at th ...
... between matched lens epithelial and cortical fiber cells. We demonstrate that 2,463 genes exhibit altered expression levels of 2 fold or greater between lens epithelial cells relative to cortical fibers. Of these, 222 genes are upregulated and 135 genes are downregulated in the lens epithelium at th ...
Nomenclature of the ARID family of DNA
... can not be established based on sequence similarity within the ARID or the presence of common conserved elements outside the ARID. Four ARID-containing proteins have been identified in Schizosaccharomyces pombe and two in Saccharomyces cerevisiae. Sequence homology within the ARID domain, orthologou ...
... can not be established based on sequence similarity within the ARID or the presence of common conserved elements outside the ARID. Four ARID-containing proteins have been identified in Schizosaccharomyces pombe and two in Saccharomyces cerevisiae. Sequence homology within the ARID domain, orthologou ...
in Power-Point Format
... • Promoters recognized by pol II (class II promoters) are similar to prokaryotic promoters: • Considered to have two parts: – Core promoter of 4 elements: TATAAA, TBP, BRE (IIB), ...
... • Promoters recognized by pol II (class II promoters) are similar to prokaryotic promoters: • Considered to have two parts: – Core promoter of 4 elements: TATAAA, TBP, BRE (IIB), ...
Phenotypic and Molecular Identification of Bifidobacterium sp
... bacteria and showed a high degree of sequence conservation the homology analysis in sequence of this gene given some interesting results for the phylogenetic analysis of the genus Bifidobacterium . ...
... bacteria and showed a high degree of sequence conservation the homology analysis in sequence of this gene given some interesting results for the phylogenetic analysis of the genus Bifidobacterium . ...
Analysis of Binary Relations and Hierarchies of Enzymes in the
... We have started a project named KEGG (Kyoto Encyclopedia of Genes and Genomes), to computerize the knowledge of the information pathways of biomolecules[6]. As an initial part of the project, we have collected and computerized the metabolic pathway data into the electronic form. The WWW implementati ...
... We have started a project named KEGG (Kyoto Encyclopedia of Genes and Genomes), to computerize the knowledge of the information pathways of biomolecules[6]. As an initial part of the project, we have collected and computerized the metabolic pathway data into the electronic form. The WWW implementati ...
The KIPHOS gene encoding a repressible acid
... treatment of the protein with endoglycosidase H). The purified enzyme shows size heterogeneity, with an apparent molecular mass in the range 90-200 kDa due to the carbohydrate content (10 putative glycosylation sites were identified in the sequence). A 16 amino acid sequence at the N-terminus is sim ...
... treatment of the protein with endoglycosidase H). The purified enzyme shows size heterogeneity, with an apparent molecular mass in the range 90-200 kDa due to the carbohydrate content (10 putative glycosylation sites were identified in the sequence). A 16 amino acid sequence at the N-terminus is sim ...
Polyunsaturated fatty acids stimulate hepatic UCP
... this hypothesis. UCP-2, like UCP-1, has been shown to be able to dissipate the proton gradient when overexpressed in yeast or reconstituted in vesicles with coenzyme Q (12, 13, 16). Also, there is indirect evidence in primary hepatocytes suggesting that UCP-2 is capable of dissipating the mitochondr ...
... this hypothesis. UCP-2, like UCP-1, has been shown to be able to dissipate the proton gradient when overexpressed in yeast or reconstituted in vesicles with coenzyme Q (12, 13, 16). Also, there is indirect evidence in primary hepatocytes suggesting that UCP-2 is capable of dissipating the mitochondr ...
video slide - SharpSchool
... From the growth patterns of the mutants, Beadle and Tatum deduced that each mutant was unable to carry out one step in the pathway for synthesizing arginine, presumably because it lacked the necessary enzyme. Because each of their mutants was mutated in a single gene, they concluded that each mutate ...
... From the growth patterns of the mutants, Beadle and Tatum deduced that each mutant was unable to carry out one step in the pathway for synthesizing arginine, presumably because it lacked the necessary enzyme. Because each of their mutants was mutated in a single gene, they concluded that each mutate ...
Unicellular Eukaryotes to Humans Protein Arginine
... chromatin formation. One of the first demonstrations of the functional consequence of PRMT1-dependent arginine methylation was on the kinetics of nucleocytoplasmic transport. Using a temperature-sensitive strain with a substitution in a nucleoporin that blocks only nuclear import, it was demonstrate ...
... chromatin formation. One of the first demonstrations of the functional consequence of PRMT1-dependent arginine methylation was on the kinetics of nucleocytoplasmic transport. Using a temperature-sensitive strain with a substitution in a nucleoporin that blocks only nuclear import, it was demonstrate ...
TriFecta Dicer-Substrate RNAi Manual
... Splice common: targets all known variants of a gene in RefSeq; duplexes lie within common exons. This constitutes the bulk of the TriFECTa Dicer-Substrate RNAi collection and is appropriate for most users. Splice specific: targets only exons present in specific splice forms. In some cases, the uniqu ...
... Splice common: targets all known variants of a gene in RefSeq; duplexes lie within common exons. This constitutes the bulk of the TriFECTa Dicer-Substrate RNAi collection and is appropriate for most users. Splice specific: targets only exons present in specific splice forms. In some cases, the uniqu ...
View/print full test page
... o Sequencing is performed using a customized next generation sequencing library. Analysis includes the coding exons of all genes in the panel plus ten bases into the introns and untranslated regions (5' and 3'). Sanger sequencing is performed to confirm variants suspected or confirmed to be pathogen ...
... o Sequencing is performed using a customized next generation sequencing library. Analysis includes the coding exons of all genes in the panel plus ten bases into the introns and untranslated regions (5' and 3'). Sanger sequencing is performed to confirm variants suspected or confirmed to be pathogen ...
Genome analysis of Desulfotomaculum gibsoniae strain
... Keywords: spore-forming anaerobes, sulfate reduction, autotrophic, anaerobic degradation of aromatic compounds, complete oxidizer, Peptococcaceae, Clostridiales Desulfotomaculum gibsoniae is a mesophilic member of the polyphyletic spore-forming genus Desulfotomaculum within the family Peptococcaceae ...
... Keywords: spore-forming anaerobes, sulfate reduction, autotrophic, anaerobic degradation of aromatic compounds, complete oxidizer, Peptococcaceae, Clostridiales Desulfotomaculum gibsoniae is a mesophilic member of the polyphyletic spore-forming genus Desulfotomaculum within the family Peptococcaceae ...
Tissue-specific codon usage and the expression of
... patterns from only two studies, we limited ourselves to fewer data than are available in large compilations of many expression studies. On the other hand, the expression data we have used are comparable (both studies used the GeneChip HuGeneFL microarray), and they provide a consistent, unbiased met ...
... patterns from only two studies, we limited ourselves to fewer data than are available in large compilations of many expression studies. On the other hand, the expression data we have used are comparable (both studies used the GeneChip HuGeneFL microarray), and they provide a consistent, unbiased met ...
Getting to the root of plant biology: impact of the Arabidopsis
... the accumulation of gene transcripts across the entire genome. This approach has already had a major impact on our understanding of gene regulation during root development. ...
... the accumulation of gene transcripts across the entire genome. This approach has already had a major impact on our understanding of gene regulation during root development. ...
Emerging real-time PCR applications.
... markers for diagnosing, staging and monitoring complex diseases. It is not meaningful to base diagnostics on global expression, since most genes are not sensitive to the disease condition and will only contribute with confounding variance to any analysis. The challenge is to select reliable panels o ...
... markers for diagnosing, staging and monitoring complex diseases. It is not meaningful to base diagnostics on global expression, since most genes are not sensitive to the disease condition and will only contribute with confounding variance to any analysis. The challenge is to select reliable panels o ...
For the last three and a half billion years, evolution has been
... documentation represents the shared rules that allow the three databases to exchange data on a daily basis. The range of features to be represented is diverse, including regions which: perform a biological function, affect or are the result of the expression of a biological function, ...
... documentation represents the shared rules that allow the three databases to exchange data on a daily basis. The range of features to be represented is diverse, including regions which: perform a biological function, affect or are the result of the expression of a biological function, ...
Characteristics of the gene encoding pyrroline-5-carboxylate synthase (P5CS) in Glycine max
... The research on proline synthesis and lysis demonstrates that proline plays a role as a primary source of energy, nitrogen and carbon in plant cells under drought conditions (Kohl et al., 1988 [15]; Kavi kishor et al., 1995 [14]; Peng et al., 1995 [16]; Hua et al., 1997 [12]). In Vigna aconitifolia ...
... The research on proline synthesis and lysis demonstrates that proline plays a role as a primary source of energy, nitrogen and carbon in plant cells under drought conditions (Kohl et al., 1988 [15]; Kavi kishor et al., 1995 [14]; Peng et al., 1995 [16]; Hua et al., 1997 [12]). In Vigna aconitifolia ...
Slide 1
... The effects of mutations on genes vary widely. Some have little or no effect; and some produce beneficial variations. Some negatively disrupt gene function. Whether a mutation is negative or beneficial depends on how its DNA changes relative to the organism’s situation. Mutations are often thought o ...
... The effects of mutations on genes vary widely. Some have little or no effect; and some produce beneficial variations. Some negatively disrupt gene function. Whether a mutation is negative or beneficial depends on how its DNA changes relative to the organism’s situation. Mutations are often thought o ...
Genetic Engineering and Genomics
... this database. As a tool for biological research, this database potentially offers new ways of studying everything else in biology. In addition, the project has spawned many practical advances in biotechnology and genetic engineering. ...
... this database. As a tool for biological research, this database potentially offers new ways of studying everything else in biology. In addition, the project has spawned many practical advances in biotechnology and genetic engineering. ...
Agrobacterium tumefaciens T DNA mediated transformation of
... marker (AmpR), to facilitate plasmid rescue in E. coli of T-DNA junction sequences containing E. festucae DNA. Plasmid pYT6 contains a hygromycin resistance cassette between the left and right T-DNA borders, a KanR bacterial selectable marker, pBR322 (ColE1) origin of replication (ori), the cis acti ...
... marker (AmpR), to facilitate plasmid rescue in E. coli of T-DNA junction sequences containing E. festucae DNA. Plasmid pYT6 contains a hygromycin resistance cassette between the left and right T-DNA borders, a KanR bacterial selectable marker, pBR322 (ColE1) origin of replication (ori), the cis acti ...
Book Review - Journal of Experimental Biology
... metabolism, along with molecular and biophysical descriptions of basic metabolic mechanisms. Chapter 1 deals with basic principles and a variety of metabolic control mechanisms, with a short description of key approaches used in the study of metabolism. As will become apparent to readers of this boo ...
... metabolism, along with molecular and biophysical descriptions of basic metabolic mechanisms. Chapter 1 deals with basic principles and a variety of metabolic control mechanisms, with a short description of key approaches used in the study of metabolism. As will become apparent to readers of this boo ...
PDF - Oxford Academic - Oxford University Press
... Vastenhouw focuses on how zebrafish studies have aided in characterizing the features and role of chromatin in transmitting epigenetic information during the maternal to zygotic transition and establishing pluripotency during early development. Genomewide DNA methylation studies in zebrafish have al ...
... Vastenhouw focuses on how zebrafish studies have aided in characterizing the features and role of chromatin in transmitting epigenetic information during the maternal to zygotic transition and establishing pluripotency during early development. Genomewide DNA methylation studies in zebrafish have al ...
Gene regulatory network
A gene regulatory network or genetic regulatory network (GRN) is a collection of regulators thatinteract with each other and with other substances in the cell to govern the gene expression levels of mRNA and proteins.The regulator can be DNA, RNA, protein and their complex. The interaction can be direct or indirect (through their transcribed RNA or translated protein).In general, each mRNA molecule goes on to make a specific protein (or set of proteins). In some cases this protein will be structural, and will accumulate at the cell membrane or within the cell to give it particular structural properties. In other cases the protein will be an enzyme, i.e., a micro-machine that catalyses a certain reaction, such as the breakdown of a food source or toxin. Some proteins though serve only to activate other genes, and these are the transcription factors that are the main players in regulatory networks or cascades. By binding to the promoter region at the start of other genes they turn them on, initiating the production of another protein, and so on. Some transcription factors are inhibitory.In single-celled organisms, regulatory networks respond to the external environment, optimising the cell at a given time for survival in this environment. Thus a yeast cell, finding itself in a sugar solution, will turn on genes to make enzymes that process the sugar to alcohol. This process, which we associate with wine-making, is how the yeast cell makes its living, gaining energy to multiply, which under normal circumstances would enhance its survival prospects.In multicellular animals the same principle has been put in the service of gene cascades that control body-shape. Each time a cell divides, two cells result which, although they contain the same genome in full, can differ in which genes are turned on and making proteins. Sometimes a 'self-sustaining feedback loop' ensures that a cell maintains its identity and passes it on. Less understood is the mechanism of epigenetics by which chromatin modification may provide cellular memory by blocking or allowing transcription. A major feature of multicellular animals is the use of morphogen gradients, which in effect provide a positioning system that tells a cell where in the body it is, and hence what sort of cell to become. A gene that is turned on in one cell may make a product that leaves the cell and diffuses through adjacent cells, entering them and turning on genes only when it is present above a certain threshold level. These cells are thus induced into a new fate, and may even generate other morphogens that signal back to the original cell. Over longer distances morphogens may use the active process of signal transduction. Such signalling controls embryogenesis, the building of a body plan from scratch through a series of sequential steps. They also control and maintain adult bodies through feedback processes, and the loss of such feedback because of a mutation can be responsible for the cell proliferation that is seen in cancer. In parallel with this process of building structure, the gene cascade turns on genes that make structural proteins that give each cell the physical properties it needs.It has been suggested that, because biological molecular interactions are intrinsically stochastic, gene networks are the result of cellular processes and not their cause (i.e. cellular Darwinism). However, recent experimental evidence has favored the attractor view of cell fates.