Biology OF CLONING VECTORS-III-B.SC - E
... strand and prevents synthesis of complementary strand. From this point on , only viral single strands are synthesized. These progeny single strands are released from the cell as filamentous particles following morphogenesis at the cell membrane. As the DNA passes through the membrane, the DNA-bindin ...
... strand and prevents synthesis of complementary strand. From this point on , only viral single strands are synthesized. These progeny single strands are released from the cell as filamentous particles following morphogenesis at the cell membrane. As the DNA passes through the membrane, the DNA-bindin ...
Probing protein function by chemical modification
... lipid or on the side chains of amino acids with recombinant Rab thioesters [23,24] (Figure 3). Dansyl and NBD fluorophores were introduced as reporters. This approach also enabled precise installation of GDP/GTP(or analog GppNHp) into Rab proteins to generate the ‘off’ and ‘on’ states, yielding homo ...
... lipid or on the side chains of amino acids with recombinant Rab thioesters [23,24] (Figure 3). Dansyl and NBD fluorophores were introduced as reporters. This approach also enabled precise installation of GDP/GTP(or analog GppNHp) into Rab proteins to generate the ‘off’ and ‘on’ states, yielding homo ...
Characterization of the first cultured representative of
... using the ‘RS_HGAP_Assembly.1’ protocol included in SMRTPortal version 2.1.1 including all five SMRT cells applying parameters described previously (Ben Hania et al., 2015), but not allowing partial alignments. Finally, one chromosomal contig could be obtained, which was trimmed, circularized and ad ...
... using the ‘RS_HGAP_Assembly.1’ protocol included in SMRTPortal version 2.1.1 including all five SMRT cells applying parameters described previously (Ben Hania et al., 2015), but not allowing partial alignments. Finally, one chromosomal contig could be obtained, which was trimmed, circularized and ad ...
Complete DNA sequence of yeast chromosome II.
... powert.ul genetic techniques; and (iii) functions in yeast have been studied in great detail biochemically. Based on present data, one can calculate that a repertoire of 65007000 genes is sufficient to build this simple eukaryotic cell. Considering recent progress and worldwide studies of yeast geno ...
... powert.ul genetic techniques; and (iii) functions in yeast have been studied in great detail biochemically. Based on present data, one can calculate that a repertoire of 65007000 genes is sufficient to build this simple eukaryotic cell. Considering recent progress and worldwide studies of yeast geno ...
2, The Glyoxylate Pathway
... • When R5P is needed more than NADPH, F6P and GAP can be diverted from the glycolytic pathway for use in synthesis of R5P by the reversal of the transaldolase and transketolase reactions. • Flux through the pathway and thus the rate of NADPH production is controlled by rate of glucose-6-phosphate de ...
... • When R5P is needed more than NADPH, F6P and GAP can be diverted from the glycolytic pathway for use in synthesis of R5P by the reversal of the transaldolase and transketolase reactions. • Flux through the pathway and thus the rate of NADPH production is controlled by rate of glucose-6-phosphate de ...
Document
... • When R5P is needed more than NADPH, F6P and GAP can be diverted from the glycolytic pathway for use in synthesis of R5P by the reversal of the transaldolase and transketolase reactions. • Flux through the pathway and thus the rate of NADPH production is controlled by rate of glucose-6-phosphate de ...
... • When R5P is needed more than NADPH, F6P and GAP can be diverted from the glycolytic pathway for use in synthesis of R5P by the reversal of the transaldolase and transketolase reactions. • Flux through the pathway and thus the rate of NADPH production is controlled by rate of glucose-6-phosphate de ...
Complete genome sequence of the rifamycin SV
... Amycolatopsis mediterranei is used for industry-scale production of rifamycin, which plays a vital role in antimycobacterial therapy. As the first sequenced genome of the genus Amycolatopsis, the chromosome of strain U32 comprising 10 236 715 base pairs, is one of the largest prokaryotic genomes eve ...
... Amycolatopsis mediterranei is used for industry-scale production of rifamycin, which plays a vital role in antimycobacterial therapy. As the first sequenced genome of the genus Amycolatopsis, the chromosome of strain U32 comprising 10 236 715 base pairs, is one of the largest prokaryotic genomes eve ...
GFP - Bio-Rad
... linking it to other DNA molecules. The basic concept behind DNA splicing is to remove a functional DNA fragment—let’s say a gene—from one organism and combine it with the DNA of another organism in order to make the protein that gene codes for. The desired result of gene splicing is for the recipien ...
... linking it to other DNA molecules. The basic concept behind DNA splicing is to remove a functional DNA fragment—let’s say a gene—from one organism and combine it with the DNA of another organism in order to make the protein that gene codes for. The desired result of gene splicing is for the recipien ...
Membrane Protein Expression in Cell
... Membrane protein synthesis by cell-free expression does not appear to be restricted by origin, size or topology of the target, and its global application is therefore a highly valuable characteristic. The technology is relatively fast to establish in standard biochemical labs, and it does not requir ...
... Membrane protein synthesis by cell-free expression does not appear to be restricted by origin, size or topology of the target, and its global application is therefore a highly valuable characteristic. The technology is relatively fast to establish in standard biochemical labs, and it does not requir ...
Coarse-grained normal mode analysis in structural biology
... are able to capture the collective dynamics of proteins. This fact has recently been applied to steer MD simulations along these dominant modes of motion using hybrid methods that combine MD and harmonic modes [43,44,45]. Specifically, a hybrid MD/NMA simulation protocol has been implemented, wher ...
... are able to capture the collective dynamics of proteins. This fact has recently been applied to steer MD simulations along these dominant modes of motion using hybrid methods that combine MD and harmonic modes [43,44,45]. Specifically, a hybrid MD/NMA simulation protocol has been implemented, wher ...
Molecular cloning and characterization of an acyl
... sequences of AhFatB1 obtained. The specificity amplification primers for AhFatB1 were FatB-F4 (forward) and FatB-R1 (reverse). The Ubiqutin gene that was previously reported as a useful internal control was used as the reference gene (Luo et al., 2005). Both of the primers used for AhFatB1 and Ubiqu ...
... sequences of AhFatB1 obtained. The specificity amplification primers for AhFatB1 were FatB-F4 (forward) and FatB-R1 (reverse). The Ubiqutin gene that was previously reported as a useful internal control was used as the reference gene (Luo et al., 2005). Both of the primers used for AhFatB1 and Ubiqu ...
Cytochrome P450 - Spektrum der Wissenschaft
... different cytochrome P450 isoforms where they are consumed in hydroxylation reactions. The yeast two-hybrid system is a technique to detect interactions between two proteins. It was developed to provide a genetic means of identifying proteins that physically interact in vivo. We adapted this system ...
... different cytochrome P450 isoforms where they are consumed in hydroxylation reactions. The yeast two-hybrid system is a technique to detect interactions between two proteins. It was developed to provide a genetic means of identifying proteins that physically interact in vivo. We adapted this system ...
Presentation (PowerPoint File)
... One other area which is even more difficult to resolve is experimental data formats, microarray, proteomic, metabolmic, basically all the omics. ...
... One other area which is even more difficult to resolve is experimental data formats, microarray, proteomic, metabolmic, basically all the omics. ...
The identification of transcription factors expressed in the notochord
... The notochord is the distinctive characteristic of chordates; however, the knowledge of the complement of transcription factors governing the development of this structure is still incomplete. Here we present the expression patterns of seven transcription factor genes detected in the notochord of th ...
... The notochord is the distinctive characteristic of chordates; however, the knowledge of the complement of transcription factors governing the development of this structure is still incomplete. Here we present the expression patterns of seven transcription factor genes detected in the notochord of th ...
Vectors for Even Larger Genomic DNA Inserts
... Plasmid avoid being lost from dividing cells by carrying partitioning systems The functions involved in these systems are called par functions cis-acting site: parS trans-acting site: parA and parB ...
... Plasmid avoid being lost from dividing cells by carrying partitioning systems The functions involved in these systems are called par functions cis-acting site: parS trans-acting site: parA and parB ...
SIRT3 - Safic-Alcan Italia
... is the third member of the mammalian sirtuin family. The SIRT3 gene encodes the protein SIRT3, which exhibits NAD+-dependent deacetylase activity. SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin c ...
... is the third member of the mammalian sirtuin family. The SIRT3 gene encodes the protein SIRT3, which exhibits NAD+-dependent deacetylase activity. SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin c ...
RNA-Quant™ cDNA Synthesis Kit
... RNA-Quant™ cDNA Synthesis Kit Efficiently make cDNA to measure any RNA by qPCR Total RNA was harvested from human HT1080 cells using standard Trizol extraction protocols. The RNA-Quant kit was used to tail and tag all RNAs into quantifiable cDNA for qPCR analysis. Sample amplification plots and spe ...
... RNA-Quant™ cDNA Synthesis Kit Efficiently make cDNA to measure any RNA by qPCR Total RNA was harvested from human HT1080 cells using standard Trizol extraction protocols. The RNA-Quant kit was used to tail and tag all RNAs into quantifiable cDNA for qPCR analysis. Sample amplification plots and spe ...
Physiological adaptations of Saccharomyces cerevisiae evolved for
... components of the central metabolic pathways were quantified, as well as essentially all components of the ribosomes, together with many representatives of structural and regulatory factors. It is likely that many more proteins of lower abundance could have been quantified in a second round of targe ...
... components of the central metabolic pathways were quantified, as well as essentially all components of the ribosomes, together with many representatives of structural and regulatory factors. It is likely that many more proteins of lower abundance could have been quantified in a second round of targe ...
Avian-to-human transmission of the PB1 gene of influenza A viruses in the 1957 and 1968 pandemics
... these new surface glycoproteins are thought to have been introduced from avian species (24), suggesting genetic reassortment between human and avian influenza viruses. Most other genes of the 1957 and 1968 pandemic strains were derived from previously circulating human viruses. One exception is the ...
... these new surface glycoproteins are thought to have been introduced from avian species (24), suggesting genetic reassortment between human and avian influenza viruses. Most other genes of the 1957 and 1968 pandemic strains were derived from previously circulating human viruses. One exception is the ...
Rapid Publication - Journal of Clinical Investigation
... levels, has approximately normal calculated specific activity, in contrast to the reduced specific activity of ADA from most other ADA-deficient cell lines (22). However, Northern blotting has previously shown that GM- 1715 contains normal to above normal levels of ADA mRNA (2, 3). Down-regulation o ...
... levels, has approximately normal calculated specific activity, in contrast to the reduced specific activity of ADA from most other ADA-deficient cell lines (22). However, Northern blotting has previously shown that GM- 1715 contains normal to above normal levels of ADA mRNA (2, 3). Down-regulation o ...
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... 20.9 The basal apparatus assembles at the promoter 20.10 Initiation is followed by promoter clearance 20.11 A connection between transcription and repair 20.12 Promoters for RNA polymerase II have short sequence elements 20.13 Some promoter-binding proteins are repressors 20.14 Enhancers contain bid ...
... 20.9 The basal apparatus assembles at the promoter 20.10 Initiation is followed by promoter clearance 20.11 A connection between transcription and repair 20.12 Promoters for RNA polymerase II have short sequence elements 20.13 Some promoter-binding proteins are repressors 20.14 Enhancers contain bid ...
BIL 250 – Genetics Krempels – Summer 2015 Study Guide for Exam I
... configurations, test cross, map function. (We did mapping only for diploid organisms, though it also can be done for haploid organisms much more easily. For this exam, concentrate on the diploid mapping we did. Know only that for haploid crosses you don’t have to worry about dominance/recessiveness: ...
... configurations, test cross, map function. (We did mapping only for diploid organisms, though it also can be done for haploid organisms much more easily. For this exam, concentrate on the diploid mapping we did. Know only that for haploid crosses you don’t have to worry about dominance/recessiveness: ...
Function and specificity of 14-3-3 proteins in the regulation of
... et al., 1999). Fusicoccin, a phytotoxin, affects PM H+ATPase-mediated processes such as stomatal opening and cell elongation, by irreversibly activating the PM H+ATPase via interaction with the auto-inhibitory domain (de Boer, 1997). The involvement of 14-3-3 proteins in this process has been invest ...
... et al., 1999). Fusicoccin, a phytotoxin, affects PM H+ATPase-mediated processes such as stomatal opening and cell elongation, by irreversibly activating the PM H+ATPase via interaction with the auto-inhibitory domain (de Boer, 1997). The involvement of 14-3-3 proteins in this process has been invest ...
video slide - Independent School District 196
... ribosomal subunit 1 A small ribosomal subunit binds to a molecule of mRNA. In a prokaryotic cell, the mRNA binding site on this subunit recognizes a specific nucleotide sequence on the mRNA just upstream of the start codon. An initiator tRNA, with the anticodon UAC, base-pairs with the start codon, ...
... ribosomal subunit 1 A small ribosomal subunit binds to a molecule of mRNA. In a prokaryotic cell, the mRNA binding site on this subunit recognizes a specific nucleotide sequence on the mRNA just upstream of the start codon. An initiator tRNA, with the anticodon UAC, base-pairs with the start codon, ...
Gene regulatory network
A gene regulatory network or genetic regulatory network (GRN) is a collection of regulators thatinteract with each other and with other substances in the cell to govern the gene expression levels of mRNA and proteins.The regulator can be DNA, RNA, protein and their complex. The interaction can be direct or indirect (through their transcribed RNA or translated protein).In general, each mRNA molecule goes on to make a specific protein (or set of proteins). In some cases this protein will be structural, and will accumulate at the cell membrane or within the cell to give it particular structural properties. In other cases the protein will be an enzyme, i.e., a micro-machine that catalyses a certain reaction, such as the breakdown of a food source or toxin. Some proteins though serve only to activate other genes, and these are the transcription factors that are the main players in regulatory networks or cascades. By binding to the promoter region at the start of other genes they turn them on, initiating the production of another protein, and so on. Some transcription factors are inhibitory.In single-celled organisms, regulatory networks respond to the external environment, optimising the cell at a given time for survival in this environment. Thus a yeast cell, finding itself in a sugar solution, will turn on genes to make enzymes that process the sugar to alcohol. This process, which we associate with wine-making, is how the yeast cell makes its living, gaining energy to multiply, which under normal circumstances would enhance its survival prospects.In multicellular animals the same principle has been put in the service of gene cascades that control body-shape. Each time a cell divides, two cells result which, although they contain the same genome in full, can differ in which genes are turned on and making proteins. Sometimes a 'self-sustaining feedback loop' ensures that a cell maintains its identity and passes it on. Less understood is the mechanism of epigenetics by which chromatin modification may provide cellular memory by blocking or allowing transcription. A major feature of multicellular animals is the use of morphogen gradients, which in effect provide a positioning system that tells a cell where in the body it is, and hence what sort of cell to become. A gene that is turned on in one cell may make a product that leaves the cell and diffuses through adjacent cells, entering them and turning on genes only when it is present above a certain threshold level. These cells are thus induced into a new fate, and may even generate other morphogens that signal back to the original cell. Over longer distances morphogens may use the active process of signal transduction. Such signalling controls embryogenesis, the building of a body plan from scratch through a series of sequential steps. They also control and maintain adult bodies through feedback processes, and the loss of such feedback because of a mutation can be responsible for the cell proliferation that is seen in cancer. In parallel with this process of building structure, the gene cascade turns on genes that make structural proteins that give each cell the physical properties it needs.It has been suggested that, because biological molecular interactions are intrinsically stochastic, gene networks are the result of cellular processes and not their cause (i.e. cellular Darwinism). However, recent experimental evidence has favored the attractor view of cell fates.