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3040 - Zhang, Hongyu
3040 - Zhang, Hongyu

... have standard secondary structures. Traditionally, loop-modelling methods were categorised into two kinds of approaches: knowledge-based approach and ab initio approach (for details, see the review section in Zhang et al., 1997). The former extracts the knowledge from the current protein structure d ...
An ideal protein provider - Research
An ideal protein provider - Research

... Zaaier,” says Frederic who manages the unit. “They have helped a few times in the past, and the result of their most recent cash injection will come to Auroville soon: a Soya cow, a machine to process the Soybeans. The machine’s filter will remove the particular smell and taste of the bean which som ...
Proteins Chapter 7
Proteins Chapter 7

... • The highest quality food proteins are those that are both very digestible and have an essential amino acid pattern closely matching the amino acid requirements of the body. • The food proteins of highest quality are: – Egg—contains the highest quality protein of all foods ...
Introduction to Protein Summit 2.0: continued exploration of the
Introduction to Protein Summit 2.0: continued exploration of the

... (AI), and Tolerable Upper Intake Level (2). The RDA is “the average daily dietary nutrient intake level sufficient to meet the nutrient requirement of nearly all (97 to 98 percent) healthy individuals in a particular life stage and gender group” (2). The RDA for protein, which is based primarily on ...
Characterization of two genes encoding the mitochondrial
Characterization of two genes encoding the mitochondrial

... The cDNA possessed a long 3'-untranslated region (868 bp), as found in many nuclear genes of C. reinhardtii (Franzén and Falk 1992). The motif TGTAA, considered to be a potential polyadenylation signal in C. reinhardtii (Silflow et al. 1985), was found 18 bases upstream from the 3'-end and also at p ...
Powerpoint
Powerpoint

... 8 x 108 dm3 mol-1 s-1 1 x 109 dm3 mol-1 s-1 7 x 108 dm3 mol-1 s-1 9 x 109 dm3 mol-1 s-1 4 x 1011 dm3 mol-1 s-1 8 x 1010 dm3 mol-1 s-1 1 x 1010 dm3 mol-1 s-1, 1.4 x 1010 dm3 mol-1 s-1, 6.9 x 109 dm3 mol-1 s-1 ...
Oxy-haemoglobin protein engineering
Oxy-haemoglobin protein engineering

... different sectors viz. biocatalyst, disease diagnosis and therapy, nanoscience etc. [12]. In general, stability means protein gets unfold and refold during unfavourable environmental conditions as temperature or solvent, etc. It is interesting to note that all proteins are simplest form and suitable ...
SCRATCH: a protein structure and structural feature prediction server
SCRATCH: a protein structure and structural feature prediction server

... total number of contacts in a band from the sum of all the predicted contact probabilities in that band. The conformational space is searched using a variant of simulated annealing, where the moves we use to modify our models are crankshaft moves (13) on one or more residues and several forms of fra ...
Gene Expression in Thyroxin-Induced Metamorphosing Axolotl Hearts
Gene Expression in Thyroxin-Induced Metamorphosing Axolotl Hearts

... The Mexican axolotl (Ambystoma mexicanum) is a unique model to study vertebrate heart development for several reasons. In addition to the wild-type animal, there is also an embryonic lethal condition caused by a homozygous recessive mutation in cardiac gene “c” [1,2]. These mutant embryonic hearts d ...
ER, Golgi and Vesicles :
ER, Golgi and Vesicles :

... Once a polypeptide has been translated and released from the ribosome, it may be ready for use, but often it must undergo post-translational processing in order to become fully functional. While many of these processes are carried out in both prokaryotes and eukaryotes, the presence of organelles pr ...
Expression of floral identity genes in Clianthus
Expression of floral identity genes in Clianthus

... development and in specific organs. However, although the ABC model has been shown to be applicable to a wide range of species (Ng and Yanofsky, 2001; Becker and Theissen, 2003), simultaneous investigation of the expression of these genes during an entire annual cycle in natural conditions is limite ...
Brooker Chapter 14
Brooker Chapter 14

... A particular enzyme appears in the cell only after the cell has been exposed to the enzyme’s substrate This observation became known as enzyme adaptation ...
The gag proteins of human immunodeficiency virus type 1
The gag proteins of human immunodeficiency virus type 1

... majority of the structural elements known to be required for HIV-particle formation are localized in the sequence of the precursor protein Pr55gag [3] which comprises the matrix protein p 17, the capsid protein p24, the nucleocapsid protein p7, the protein p6 known as the link-protein and two smalle ...
A minimal gene set for cellular life derived by comparison of
A minimal gene set for cellular life derived by comparison of

... study. In addition, coding regions for two genes were extended. All data base screening was against the protein and nucleotide versions of the daily updated nonredundant sequence data base maintained at the National Center for Biotechnology Information. ...
Anne Ye - A Critical Review of Computational Protein Design Strategies: Progress, Limitations, and Improvements
Anne Ye - A Critical Review of Computational Protein Design Strategies: Progress, Limitations, and Improvements

... elegant molecular designs found in nature. At the same time, from a practical standpoint, protein design offers the tantalizing opportunity to unlock the potentially unlimited structural and functional space not sampled by nature. Proteins, both natural and evolved, have demonstrated tremendous vers ...
Molecular Cloning and Expression of a 2-Arylpropionyl
Molecular Cloning and Expression of a 2-Arylpropionyl

... amplification, with primers incorporating the desired sequences, which included a BamHI site in front of the starting methionine codon and a SacI site 39 behind the termination codon. The PCR product was ligated directly into the pCRII vector (TA Cloning Kit; Invitrogen, Leek, The Netherlands) and t ...
K-12 MG1655 Escherichia coli Blocks the Aerobic
K-12 MG1655 Escherichia coli Blocks the Aerobic

... the sample A260/A280 ratio (⬎1.8). cDNA preparation was performed as described by Cho et al. (7). Affymetrix GeneChip E. coli Genome 2.0 arrays were used for genome-scale transcriptional analyses. cDNA synthesis, fragmentation, end-terminus biotin labeling, and array hybridization were performed as ...
Eukaryotic-type protein kinases in Streptomyces
Eukaryotic-type protein kinases in Streptomyces

... possible roles for these enzymes in the regulation of cellular processes in streptomycetes. The S. coelicolor A3(2) genome carries 34 putative ESTPK genes Based on the screening of the chromosome sequence data (Bentley et al., 2002) using the standard BLAST program (Altschul et al., 1990) with some ...
Protein-A Science-Based Approach By Dr. Joe Klemczewski
Protein-A Science-Based Approach By Dr. Joe Klemczewski

... be superior. And it is great for speedy uptake. But isolates fell out of favor as heavier molecular weight proteins showed a longer span of nitrogen elevation because of slower digestion. Currently, the tables have turned again—somewhat—as the faster-acting isolates are showing an ability to reduce ...
Name: Date: ______ NUID
Name: Date: ______ NUID

... SDS or isoelectric focusing or both. Since the biochemist presumably knows what species they are working with, they only need a small amount of sequence to correctly identify the protein. In this circumstance, either Edman degradation or mass spectrometry may be performed. However, mass spectrometry ...
How Much Protein? - Hammer Nutrition
How Much Protein? - Hammer Nutrition

... Research confirms that rate of recovery and muscle synthesis rate are dramatically increased if amino acids and carbohydrates is consumed together immediately after the exercise training. Intense training may increase dietary protein requirements to as high as 1.7 grams protein per kilogram bodyweig ...
Awan, Ali: In Silico Transfer of Ligand Binding Function between Structurally Analogous Proteins
Awan, Ali: In Silico Transfer of Ligand Binding Function between Structurally Analogous Proteins

... protein modification has been used to alter protein performance for industrial purposes. It has been possible to change the rates, temperatures and pHs at which enzymes operate, to increase efficiency and applicability. Further, it has become possible to modify not only protein performance, but even ...
The sequence of the tms transcript 2 locus of the A. tumefaciens
The sequence of the tms transcript 2 locus of the A. tumefaciens

... shown that coupled j ^ vitro transcription/translation systems prepared from both J^ coli and A_^ tumefaciens express the 49 Kd protein, albeit poorly. It was therefore of interest to determine whether the gene had sequences typical of other procaryotic transcription/translation signals. In E_;_ col ...
Biology - Kenyon College
Biology - Kenyon College

... Acetate and formate are major fermentation products of Escherichia coli. Below pH 7, the balance shifts to lactate; an oversupply of acetate or formate retards growth. E. coli W3110 was grown with aeration in potassium-modified Luria broth buffered at pH 6.7 in the presence or absence of added aceta ...
Structural Location of Disease-Associated Single Nucleotide
Structural Location of Disease-Associated Single Nucleotide

... important role in providing genetic functional diversity. To understand the structural characteristics of disease-associated SNPs, we have mapped a set of nsSNPs derived from the Online Mendelian Inheritance in Man (OMIM) database to the structural surfaces of encoded proteins. These nsSNPs are dise ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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