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... Protein structure and human disease In some cases, a single amino acid substitution can induce a dramatic change in protein structure. For example, the DF508 mutation of CFTR alters the a helical content of the protein, and disrupts ...
... Protein structure and human disease In some cases, a single amino acid substitution can induce a dramatic change in protein structure. For example, the DF508 mutation of CFTR alters the a helical content of the protein, and disrupts ...
Identification and Developmental Expression of a Novel Low
... filament proteins in the nervous system of X. laevis (Szaro and Gainer, 1988a,b; Szaro et al., 1989) were used. The mouse monoclonal antibody aIFA is directed against an epitope common to nearly all intermediate filament proteins, which is located at the carboxyl end of the a-helical rod domain (Pru ...
... filament proteins in the nervous system of X. laevis (Szaro and Gainer, 1988a,b; Szaro et al., 1989) were used. The mouse monoclonal antibody aIFA is directed against an epitope common to nearly all intermediate filament proteins, which is located at the carboxyl end of the a-helical rod domain (Pru ...
Protein folding: looping from hydrophobic nuclei.
... size closed loops of 25-30 amino acid residues1 irrespective of details of secondary structure. The end-to-end contacts in the loops are likely to be hydrophobic, which is a testable hypothesis. This notion could be verified by direct comparison of the loop maps with Kyte and Doollitle hydropathicit ...
... size closed loops of 25-30 amino acid residues1 irrespective of details of secondary structure. The end-to-end contacts in the loops are likely to be hydrophobic, which is a testable hypothesis. This notion could be verified by direct comparison of the loop maps with Kyte and Doollitle hydropathicit ...
A Superfamily of S Locus-Related Sequences in
... that spans most of the ARK2 gene and its downstream flanking sequences. Similarly, for sequence analysis of the ARK3 gene in genomic region 4, we generated subclone pDM2 that contains the 5.5-kb BamHI-Pstl fragment that spans the entire ARK3 gene and its flanking regions. Alignment of the ARK2 and A ...
... that spans most of the ARK2 gene and its downstream flanking sequences. Similarly, for sequence analysis of the ARK3 gene in genomic region 4, we generated subclone pDM2 that contains the 5.5-kb BamHI-Pstl fragment that spans the entire ARK3 gene and its flanking regions. Alignment of the ARK2 and A ...
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... and blue, respectively, indicating that all these components are glycosylated and may have cation-binding potential. PAGE under non-denaturing conditions revealed a similar gel pattern as in SDS PAGE, confirming that these proteins are highly acidic. N-terminal sequencing of the three major componen ...
... and blue, respectively, indicating that all these components are glycosylated and may have cation-binding potential. PAGE under non-denaturing conditions revealed a similar gel pattern as in SDS PAGE, confirming that these proteins are highly acidic. N-terminal sequencing of the three major componen ...
Identification of the tRNA-binding Protein Arc1p as a Novel Target of
... Yeast Strains, Vectors, and Media—The S. cerevisiae strains used in this study are listed in Table I. For recombinant plasmid constructions, the S. cerevisiae/E. coli shuttle vectors YCplac33 (14), p414MET25 (15), pVT-100U (16), pTRC-HISA (Invitrogen) and the E. coli plasmid pQE70 (Qiagen) served as ...
... Yeast Strains, Vectors, and Media—The S. cerevisiae strains used in this study are listed in Table I. For recombinant plasmid constructions, the S. cerevisiae/E. coli shuttle vectors YCplac33 (14), p414MET25 (15), pVT-100U (16), pTRC-HISA (Invitrogen) and the E. coli plasmid pQE70 (Qiagen) served as ...
Type="Italic">AtGRP7 is involved in the
... A family of proteins consisting of one amino-terminal consensus sequence RNAbinding domain and one carboxyl-terminal glycine-rich domain was isolated from cyanobacterium [10-11], plant [12-15], and human [16] cells. It is referred to as a glycine-rich RNA-binding protein (GR-RBP) family [13, 15]. Th ...
... A family of proteins consisting of one amino-terminal consensus sequence RNAbinding domain and one carboxyl-terminal glycine-rich domain was isolated from cyanobacterium [10-11], plant [12-15], and human [16] cells. It is referred to as a glycine-rich RNA-binding protein (GR-RBP) family [13, 15]. Th ...
Global Proteomics of the Extremophile Black Fungus Cryomyces
... At the same time the described characteristics of these fungi make it difficult to perform analysis on the cellular level. For instance the cell wall is the main obstacle in protein extraction from fungal biomass. Moreover, due to rigid cell wall and melanin content, also DNA extraction and purifica ...
... At the same time the described characteristics of these fungi make it difficult to perform analysis on the cellular level. For instance the cell wall is the main obstacle in protein extraction from fungal biomass. Moreover, due to rigid cell wall and melanin content, also DNA extraction and purifica ...
SOURCES OF OUR OBJECTIONS Series A
... Proteins are chains of amino acid molecules connected by peptide bonds. There are many types of proteins and each has its own amino acid sequence (typically containing hundreds of amino acids). There are 22 different amino acids that can be combined to form protein chains. There are 9 amino acids th ...
... Proteins are chains of amino acid molecules connected by peptide bonds. There are many types of proteins and each has its own amino acid sequence (typically containing hundreds of amino acids). There are 22 different amino acids that can be combined to form protein chains. There are 9 amino acids th ...
Identification and Characterization of KLK-L4, a New Kallikrein
... down-regulated during breast cancer progression. The zyme/ protease M/neurosin gene is expressed in primary breast cancers but is down-regulated at metastatic sites (4). The large size of the kallikrein gene family in other species, such as rat and mouse, where kallikreins are encoded by 13–24 genes ...
... down-regulated during breast cancer progression. The zyme/ protease M/neurosin gene is expressed in primary breast cancers but is down-regulated at metastatic sites (4). The large size of the kallikrein gene family in other species, such as rat and mouse, where kallikreins are encoded by 13–24 genes ...
041118-SusanWong
... Train representatives of the ministries of health and education of 21 countries ...
... Train representatives of the ministries of health and education of 21 countries ...
Cloning, Purification, and Partial Characterization of the
... The Halobacterium sp. NRC-1 MCM gene was amplified using PCR from genomic DNA (kindly provided by Brian Berquist). The PCR reaction was performed with 100 ng genomic DNA, 200 M dNTPs, 5 units of OptimaseTM polymerase (Transgenomic), 1 x Optimase reaction buffer, 1.5 mM MgSO4, 10% DMSO and 0.4 M of ...
... The Halobacterium sp. NRC-1 MCM gene was amplified using PCR from genomic DNA (kindly provided by Brian Berquist). The PCR reaction was performed with 100 ng genomic DNA, 200 M dNTPs, 5 units of OptimaseTM polymerase (Transgenomic), 1 x Optimase reaction buffer, 1.5 mM MgSO4, 10% DMSO and 0.4 M of ...
Gene Section RBBP8 (retinoblastoma binding protein 8) Atlas of Genetics and Cytogenetics
... Knockout mice experiment has shown that inactivation of both CtIP alleles resulted in early embryonic lethality at stage E4. However the heterozygous CtIP+/mice were viable with reduced life span due to development of multiple tumors. ...
... Knockout mice experiment has shown that inactivation of both CtIP alleles resulted in early embryonic lethality at stage E4. However the heterozygous CtIP+/mice were viable with reduced life span due to development of multiple tumors. ...
Constitutive expression of RyhB regulates the heme biosynthesis
... sdhCDAB site of the TCA cycle, resulting in the accumulation of succinate and glutamate in E. coli DALRA. To confirm it, we performed a qRT-PCR analysis. Among the genes analyzed in the TCA cycle, sdhB was the most affected (Fig. 4). The relative transcription level of sdhB was down-regulated to onl ...
... sdhCDAB site of the TCA cycle, resulting in the accumulation of succinate and glutamate in E. coli DALRA. To confirm it, we performed a qRT-PCR analysis. Among the genes analyzed in the TCA cycle, sdhB was the most affected (Fig. 4). The relative transcription level of sdhB was down-regulated to onl ...
The balancing act between the cellular processes of protein
... resistance exercise training and/or leucine-enriched essential amino acid supplementation remains to be determined. The review by Rose and Richter (6) focuses on molecular signaling in muscle during (rather than after) exercise that contributes to reducing global muscle protein synthesis during exer ...
... resistance exercise training and/or leucine-enriched essential amino acid supplementation remains to be determined. The review by Rose and Richter (6) focuses on molecular signaling in muscle during (rather than after) exercise that contributes to reducing global muscle protein synthesis during exer ...
Improving the Content of Essential Amino Acids in
... of recombinant genes encoding Lys-rich proteins have been tested so far: (1) natural genes encoding Lys-rich proteins derived from different plant or nonplant sources; (2) natural genes that have been mutated to increase the number of Lys codons and make proteins richer in Lys; and (3) synthetic gen ...
... of recombinant genes encoding Lys-rich proteins have been tested so far: (1) natural genes encoding Lys-rich proteins derived from different plant or nonplant sources; (2) natural genes that have been mutated to increase the number of Lys codons and make proteins richer in Lys; and (3) synthetic gen ...
Function and specificity of 14-3-3 proteins in the regulation of
... 1993; Toroser and Huber, 1997). However, there is a sitespeci®c regulatory interaction between 14-3-3 proteins and Ser-229 of spinach SPS which inhibits SPS activity (Toroser et al., 1998). The interaction of 14-3-3s and target proteins can be reduced in vitro by 5¢-AMP which interacts with 14-3-3s ...
... 1993; Toroser and Huber, 1997). However, there is a sitespeci®c regulatory interaction between 14-3-3 proteins and Ser-229 of spinach SPS which inhibits SPS activity (Toroser et al., 1998). The interaction of 14-3-3s and target proteins can be reduced in vitro by 5¢-AMP which interacts with 14-3-3s ...
Protein nutritional value of rocket leaves and
... 1%–2% protein, although the content of this component in some vegetables is much higher (Kunachowicz et al., 2005). Plant proteins, even though they do not contain a sufficient amount of indispensable amino acids, are recommended in Western-type diets as a way to reduce the risk of chronic diseases ...
... 1%–2% protein, although the content of this component in some vegetables is much higher (Kunachowicz et al., 2005). Plant proteins, even though they do not contain a sufficient amount of indispensable amino acids, are recommended in Western-type diets as a way to reduce the risk of chronic diseases ...
A “Tag-and-Modify” Approach to Site
... several post-translational modifications (Scheme 4). However, when only efficient conjugation is required, rather than post-translational mimicry, other methods allow access to S-linked modifications. The addition of thiyl radicals to alkenetagged amino acids such as homoallylglycine (Hag) is one me ...
... several post-translational modifications (Scheme 4). However, when only efficient conjugation is required, rather than post-translational mimicry, other methods allow access to S-linked modifications. The addition of thiyl radicals to alkenetagged amino acids such as homoallylglycine (Hag) is one me ...
Discovery, Structural Determination, and Putative
... inhibitors (BBIs) that are widely distributed within plants of the Fabaceae (leguminous) and Poaceae (graminaceous) families (7, 8). Two classes of BBIs have been identified as follows: the first class, found in the Poaceae, possessing a single active site, and the second class, found in both plant ...
... inhibitors (BBIs) that are widely distributed within plants of the Fabaceae (leguminous) and Poaceae (graminaceous) families (7, 8). Two classes of BBIs have been identified as follows: the first class, found in the Poaceae, possessing a single active site, and the second class, found in both plant ...
Sequence Motif Identification and Protein Family - IME-USP
... for every symbol x, the leaf x is added to t, if the sequence xt appears in the training sequences at least Nmin times. For every pair of new leaves of a node, we test their equivalence using a log-likelihood ratio test and choose the pair that realizes the minimum between all the tests. If this min ...
... for every symbol x, the leaf x is added to t, if the sequence xt appears in the training sequences at least Nmin times. For every pair of new leaves of a node, we test their equivalence using a log-likelihood ratio test and choose the pair that realizes the minimum between all the tests. If this min ...
Impact of glucose uptake rate on recombinant protein production in
... Escherichia coli (E. coli) is an attractive host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve high product quantities. In this thesis, the impact of the glucose uptake rate on the production of recombinant proteins was studied, aiming at ...
... Escherichia coli (E. coli) is an attractive host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve high product quantities. In this thesis, the impact of the glucose uptake rate on the production of recombinant proteins was studied, aiming at ...
Electrophoretic Analysis of Native Proteins
... The migration of a protein through an agarose gel is dependent primarily on the net electric charge on the protein, the intensity of the electric field, and the pH and ionic strength of the buffer. The pH of the electrophoresis buffer is critical because it determines the net charge on the protein m ...
... The migration of a protein through an agarose gel is dependent primarily on the net electric charge on the protein, the intensity of the electric field, and the pH and ionic strength of the buffer. The pH of the electrophoresis buffer is critical because it determines the net charge on the protein m ...
Table S6: Domains present in the primary network generated from
... is thought interact with FtsZ. Thought to catalyse 2-phosphosulpholactate = sulpholactate + phosphate. Probable magnesium cofactor. Involved in the second step of coenzyme M biosynthesis. Inhibited by vanadate in Methanococcus jannaschii. Also known as the ComB family. CbiD is essential for cobalami ...
... is thought interact with FtsZ. Thought to catalyse 2-phosphosulpholactate = sulpholactate + phosphate. Probable magnesium cofactor. Involved in the second step of coenzyme M biosynthesis. Inhibited by vanadate in Methanococcus jannaschii. Also known as the ComB family. CbiD is essential for cobalami ...