In silico Prediction and Docking of Tertiary Structure of LuxI, an
... Conclusion: The model produced, and the predicted acteyltransferase binding site, could be useful in modeling homologous sequences from other microorganisms and the design of new antimicrobials. Keywords: Docking, Homology modeling, LuxI, Quorum sensing ...
... Conclusion: The model produced, and the predicted acteyltransferase binding site, could be useful in modeling homologous sequences from other microorganisms and the design of new antimicrobials. Keywords: Docking, Homology modeling, LuxI, Quorum sensing ...
Protein Crystallization
... All entropic phenomena associated with the protein during crystallization are unfavorable: ¾ loss of degrees of freedom due to incorporation of molecules into the lattice which is an unavoidable entropic cost of crystallization; 30 – 100 kJ mol-1 at room T ¾ loss of entropy due to ordering of otherw ...
... All entropic phenomena associated with the protein during crystallization are unfavorable: ¾ loss of degrees of freedom due to incorporation of molecules into the lattice which is an unavoidable entropic cost of crystallization; 30 – 100 kJ mol-1 at room T ¾ loss of entropy due to ordering of otherw ...
Protein Conformation and Function
... provide variety and determine the chemical and physical properties. ...
... provide variety and determine the chemical and physical properties. ...
The Crystal Structure of Arabidopsis thaliana Allene Oxide Cyclase
... an apparent mass of ;22 kD, the N-terminally tagged protein shows a dominant band at a molecular mass of ;60 kD, consistent with an SDS-stable trimer formation. The identity of the bands could be confirmed by immunoblot analyses using antibodies raised against AOC2 or the His6-tag. A similar pattern ...
... an apparent mass of ;22 kD, the N-terminally tagged protein shows a dominant band at a molecular mass of ;60 kD, consistent with an SDS-stable trimer formation. The identity of the bands could be confirmed by immunoblot analyses using antibodies raised against AOC2 or the His6-tag. A similar pattern ...
CYP74C3 and CYP74A1, plant cytochrome P450 enzymes whose
... [8–10]. HPL is present at only very low levels in plants and is relatively unstable [9], so the subsequent conversion of the hydroperoxides into volatile aldehydes is usually carried out by a recombinant HPL present in crude Escherichia coli [8] or yeast [10] extracts; the latter would be more costl ...
... [8–10]. HPL is present at only very low levels in plants and is relatively unstable [9], so the subsequent conversion of the hydroperoxides into volatile aldehydes is usually carried out by a recombinant HPL present in crude Escherichia coli [8] or yeast [10] extracts; the latter would be more costl ...
Novel eukaryotic enzymes modifying cell
... homologs of this protein were also found in animals and plants suggesting that such modifications might have a more general function in cell-surface adhesion across eukaryotes. Evidence from Cryptococcus indicates that the multi-TM protein Cas1p is the acyltransferase that adds the acetyl groups to ...
... homologs of this protein were also found in animals and plants suggesting that such modifications might have a more general function in cell-surface adhesion across eukaryotes. Evidence from Cryptococcus indicates that the multi-TM protein Cas1p is the acyltransferase that adds the acetyl groups to ...
Protein Data Condensation for Effective Quaternary Structure
... that are, homodimers and non-homodimers, whereas our approach is able to discriminate among any number of classes. In this respect, our method is more similar to the two approaches illustrated in the following. The main contribution of the work [5] is the exploitation of a pseudo amino acid composit ...
... that are, homodimers and non-homodimers, whereas our approach is able to discriminate among any number of classes. In this respect, our method is more similar to the two approaches illustrated in the following. The main contribution of the work [5] is the exploitation of a pseudo amino acid composit ...
Finding of a novel fungal immunomodulatory protein coding
... (Chang et al., 2009; Lin et al., 2006); in addition, its metabolites can be effective antivirals against the human immunodeficiency virus (HIV) (Gao et al., 2003). Recently, one of these species’ bioactive therapeutic proteins has been cloned and efficiently expressed in molecular vectors (Wu et al ...
... (Chang et al., 2009; Lin et al., 2006); in addition, its metabolites can be effective antivirals against the human immunodeficiency virus (HIV) (Gao et al., 2003). Recently, one of these species’ bioactive therapeutic proteins has been cloned and efficiently expressed in molecular vectors (Wu et al ...
Function of lanI in regulation of landomycin A biosynthesis in
... by transcriptional factors which genes are clustered with the respective antibiotic biosynthesis genes. Members of this big group of proteins resembles the OmpR transcriptional regulator of Escherichia coli phosphate regulon particularly in the region of DNA-binding domain formed by “winged” helix-t ...
... by transcriptional factors which genes are clustered with the respective antibiotic biosynthesis genes. Members of this big group of proteins resembles the OmpR transcriptional regulator of Escherichia coli phosphate regulon particularly in the region of DNA-binding domain formed by “winged” helix-t ...
Transcriptome analysis of Sacha Inchi (Plukenetia volubilis L
... this plant are rich in unsaturated fatty acids (FAs). In particular, the fatty acid composition of its seed oil differs markedly in containing large quantities of α-linolenic acid (18C:3, a kind of ω-3 FAs). However, little is known about the molecular mechanisms responsible for biosynthesis of unsa ...
... this plant are rich in unsaturated fatty acids (FAs). In particular, the fatty acid composition of its seed oil differs markedly in containing large quantities of α-linolenic acid (18C:3, a kind of ω-3 FAs). However, little is known about the molecular mechanisms responsible for biosynthesis of unsa ...
Use of the non-radioactive SUnSET method to detect decreased
... reviewed [20], and has been widely employed in human cell lines [21] and human tissue, including skeletal muscle [22] and the hippocampus [23]; however, SUnSET has not been demonstrated in plants. Briefly, this technique requires treatment and uptake of the antibiotic puromycin, a structural analogu ...
... reviewed [20], and has been widely employed in human cell lines [21] and human tissue, including skeletal muscle [22] and the hippocampus [23]; however, SUnSET has not been demonstrated in plants. Briefly, this technique requires treatment and uptake of the antibiotic puromycin, a structural analogu ...
35 USC §112 Written Description
... • Data from animal testing is relevant to asserted human therapeutic utility if there is a “satisfactory correlation” between the effect on animals and humans • Do not need to prove correlation with statistical certainty • If no previous treatment or cure, the PTO will carefully review for credibili ...
... • Data from animal testing is relevant to asserted human therapeutic utility if there is a “satisfactory correlation” between the effect on animals and humans • Do not need to prove correlation with statistical certainty • If no previous treatment or cure, the PTO will carefully review for credibili ...
Lecture Notes - Math
... The Tertiary structure of proteins There are a wide variety of ways in which the various helix, sheets & loop elements can combine to produce a complete structure. At the level of tertiary structure, the side chains play a much more active role in creating the final structure. ...
... The Tertiary structure of proteins There are a wide variety of ways in which the various helix, sheets & loop elements can combine to produce a complete structure. At the level of tertiary structure, the side chains play a much more active role in creating the final structure. ...
Career of Tom Muir
... ■ MBP and His peptide fragments ■ Fragemnts expressed in E. coli ■ No reaction without rapamycin peptide ...
... ■ MBP and His peptide fragments ■ Fragemnts expressed in E. coli ■ No reaction without rapamycin peptide ...
Sequence-Specific Resonance Assignments of the `H
... as the simian immunodeficiency virus (SIV), the bovine immunodeficiency virus (BIV), the feline immunodeficiency virus (FIV), the sheep visna virus (VILV), the caprine arthritis encephalitisvirus (CAEV), and the ovine lentivirus (OMVV). Retroviruses use a complex machinery of RNA-binding proteins to ...
... as the simian immunodeficiency virus (SIV), the bovine immunodeficiency virus (BIV), the feline immunodeficiency virus (FIV), the sheep visna virus (VILV), the caprine arthritis encephalitisvirus (CAEV), and the ovine lentivirus (OMVV). Retroviruses use a complex machinery of RNA-binding proteins to ...
BIOLOGY
... original polypeptide by proteases to produce a smaller product. Other modifications include the addition of sugar molecules to certain amino acids in the protein (glycosylation), or the addition of a phosphate group (phosphorylation) or a sulfate group (sulfation). Many proteins are modified by prot ...
... original polypeptide by proteases to produce a smaller product. Other modifications include the addition of sugar molecules to certain amino acids in the protein (glycosylation), or the addition of a phosphate group (phosphorylation) or a sulfate group (sulfation). Many proteins are modified by prot ...
Sequence, expression, and characterization of the first archaeal ATP
... and eukaryal species. A general feature of most ATPPFKs from the domains of Bacteria and Eukarya is their homotetrameric stucture and the allosteric regulation of activity by compounds of intermediary metabolism. The bacterial enzymes are usually composed of 34-kDa subunits, allosterically activated ...
... and eukaryal species. A general feature of most ATPPFKs from the domains of Bacteria and Eukarya is their homotetrameric stucture and the allosteric regulation of activity by compounds of intermediary metabolism. The bacterial enzymes are usually composed of 34-kDa subunits, allosterically activated ...
Systems Biology of Biological Nitrogen Fixation
... alternative defense mechanism against oxidative species. One of them prevailing in freeliving conditions, with catalase-peroxidase as main component, while the other mechanisms in symbiosis is based on peroxiredoxins as participants, this latter similar to S. meliloti where was speculate that SodC m ...
... alternative defense mechanism against oxidative species. One of them prevailing in freeliving conditions, with catalase-peroxidase as main component, while the other mechanisms in symbiosis is based on peroxiredoxins as participants, this latter similar to S. meliloti where was speculate that SodC m ...
Identification of Antigenic Regions of Duck Hepatitis B Virus Core
... high levels of 32-kDa protein, which was reactive with a specific anti-DHBc rabbit antibody, were observed in these cells beginning already at 24 h and accumulating in the cells up to 72 h posttransfection. This protein comigrates with the 32-kDa DHBc protein from cells transfected with the pCMV-DHB ...
... high levels of 32-kDa protein, which was reactive with a specific anti-DHBc rabbit antibody, were observed in these cells beginning already at 24 h and accumulating in the cells up to 72 h posttransfection. This protein comigrates with the 32-kDa DHBc protein from cells transfected with the pCMV-DHB ...
A Proteome Reference Map and Proteomic Analysis
... Primer specific to sequences within 16 S rDNA of B. longum and designed to produce a cDNA of 831 bp. against the database of B. longum NCC2705 (Version 050126, 1727 sequences) and NCBInr (Version 050623, 2,564,994 sequences); the research results were checked using Mascot with free access on the Int ...
... Primer specific to sequences within 16 S rDNA of B. longum and designed to produce a cDNA of 831 bp. against the database of B. longum NCC2705 (Version 050126, 1727 sequences) and NCBInr (Version 050623, 2,564,994 sequences); the research results were checked using Mascot with free access on the Int ...
Document
... The ALD protein: What does it do? Recent work from the labs of Ronald Wanders and Stefan Kemp in Amsterdam show that the ALD protein transports the “activated” form of VLCFA (VLCFA-CoA) into peroxisomes, where VLCFA breakdown ...
... The ALD protein: What does it do? Recent work from the labs of Ronald Wanders and Stefan Kemp in Amsterdam show that the ALD protein transports the “activated” form of VLCFA (VLCFA-CoA) into peroxisomes, where VLCFA breakdown ...
PROTEIN CHEMISTRY
... including enzymes and structural proteins, which allow the various processes associated with life to occur ...
... including enzymes and structural proteins, which allow the various processes associated with life to occur ...
Thermodynamic prediction of protein neutrality
... substitutions is of fundamental importance in understanding natural protein evolution, developing protein engineering strategies, and understanding the basis of genetic diseases. Computational and experimental studies have demonstrated that both protein stability and structure affect a protein’s tol ...
... substitutions is of fundamental importance in understanding natural protein evolution, developing protein engineering strategies, and understanding the basis of genetic diseases. Computational and experimental studies have demonstrated that both protein stability and structure affect a protein’s tol ...