• Study Resource
  • Explore
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
מצגת של PowerPoint - The ICNC PhD Program
מצגת של PowerPoint - The ICNC PhD Program

... “Creating a new theory is not like destroying an old barn and erecting a skyscraper in its place. It is rather like climbing a mountain, gaining new and wider views, discovering unexpected connections between our starting points and its rich environment. But the point from which we started out still ...
1 Protein structure Protein folding
1 Protein structure Protein folding

... There is also other information in DNA, e.g. – Signals regarding when a protein should be produced – Functional (not messenger) RNAs ...
CSIR researchers have proved it`s possible to manipulate bacterial
CSIR researchers have proved it`s possible to manipulate bacterial

... Microbial cell surface display is the anchoring of a heterologous protein or peptide (passenger) to the outside of the cell wall as a fusion to a cell surface associated protein (carrier). This technology has been used extensively for both eukaryotic and prokaryotic systems but has mainly focused ar ...
Slide 1
Slide 1

... P Proline ...
Regulation of Gene Expression
Regulation of Gene Expression

... respond to such stimuli, eg plants, another level of gene regulation is involved. For multicellular organisms the right gene must be activated at the right time in the right cell. Examples of gene regulation at all levels have been documented, transcriptional ...
6 Visualising Green Fluorescent Protein
6 Visualising Green Fluorescent Protein

... using a Syngene image capture system Introduction GFP is isolated from the Jellyfish Aequorea Victoria and is a member of a family of fluorescent proteins that have become widely used tools in many biological research applications. GFP is a 27KDa protein which is intrinsically fluorescent and requir ...
here
here

... assays in combination with EGF to validate the earlier observations. A new approach to this line of research will include cloning and sequencing the bovine EGF receptor and using PCR-RACE to find any RNA transcripts of soluble EGF receptors (sEGF-R). sEGF-R have been cloned and sequenced from mice a ...
Slide 1
Slide 1

... 1.5 Construct in both cases sequence logo and frequency plot. Can you identify (regulatory) sequence motifs? ...
CELL-FREE SYSTEMS FOR STUDYING THE COMPONENTS AND
CELL-FREE SYSTEMS FOR STUDYING THE COMPONENTS AND

... concentrations of ligand are present, mutant yeast cells (with a temperature-sensitive mutation in a gene involved in vesicular transport) are transfected with a yeast plasmid vector into which random normal yeast genomic DNA fragments have been cloned. Because these plasmids are maintained in cells ...
Multipower Sportsfood launches Fit Protein Lite
Multipower Sportsfood launches Fit Protein Lite

... fat and reduced carbohydrate formula helps users to meet their protein intake goals whilst not over-consuming fat or carbohydrate, making it a flexible and convenient option.” Marketing Manager of Multipower UK, Shân Savage said: “Fit Protein Lite represents a strong addition to the Fit Protein rang ...
Synthetic Consordium for Cellulose Hydrolysis and Ethanol Production
Synthetic Consordium for Cellulose Hydrolysis and Ethanol Production

... yeast has already been achieved by Riaan Den Haan, et al in Hydrolysis and fermentation of amorphous cellulose by recombinant Saccharomyces cerevisiae. However, Riann Den Haan’s method is inefficient and expensive due to the extra steps necessary to create a suitable environment for the conversion p ...
Biotechnology Unit 3: DNA to Proteins Essential Cell Biology
Biotechnology Unit 3: DNA to Proteins Essential Cell Biology

... ii. Each protein will fold into a final __________________ called a __________________ based on its amino acid sequence 1. Proteins will naturally fold into the __________________ possible __________________ conformation 2. Each protein has one __________________ stable conformation, but there can b ...
synthetic gene networks that count
synthetic gene networks that count

... GFP fluorescence ratios between the single-inducer DIC three-counter exposed to three pulses of arabinose (N) versus two pulses of arabinose (N – 1) with varying arabinose pulse lengths and intervals; experimental results are represented by black dots. ...
File
File

... shape after denaturation, but others cannot, especially in the crowded environment of the cell. ...
Tertiary Structure to X-Ray Crystallography
Tertiary Structure to X-Ray Crystallography

... structure. This bound molecule blocks of the function of adenosine deaminase and inhibits its function. Since tertiary structure describes the three-dimensional shape of a protein, another level of protein structure is not obvious. The final level, quaternary structure, accounts for the fact that so ...
Design of Genetic Sequences Encoding MMP-2-degradable
Design of Genetic Sequences Encoding MMP-2-degradable

... extension PCR, ligated into a plasmid cloning vector, and transformed into E. •Possible solution is to insert a protein gel scaffold composed of peptides cleavable by matrix metalloproteinase-2 (MMP-2), an enzyme overactivated by coli. This method allows for the creation of polymer proteins consisti ...
MicroScale Thermophoresis Measurements on in vitro Synthesized
MicroScale Thermophoresis Measurements on in vitro Synthesized

... highly pure sample and a successful and time consuming immobilization strategy. ITC requires vast amounts of material that can be a challenge to produce at sufficient high quality. The present study was designed to show that the novel and powerful technology MST used in combination with cell-free pr ...
Protein Expression and Purification Service Quotation Request Form
Protein Expression and Purification Service Quotation Request Form

... If several applications are needed, please mention the preferred one below (if any): What kind of sample will the antibody be used on? Additional relevant information regarding the final application, the target protein, the hybridoma development process… Final amount of purified antibody required: S ...
Tasks Monday January 21st 2006
Tasks Monday January 21st 2006

... other 'kingdoms'. You will collect information for these homologs (e.g. protein size, protein domains present). Using this information, you will try to find out the possible evolution for this gene and how it did arise in various organisms. Find the amino acid sequence of the E. coli photolyase pro ...
Media:RuthNov07pres
Media:RuthNov07pres

... protein levels in a model DHC strain (D. ethenogenes strain 195 - DET) growing in mixed-culture conditions in pseudo-steady-state reactors and to utilize systems biology algorithms of network inference to compile the data into a model ...
BIOCHEMISTRY REVIEW SHEET
BIOCHEMISTRY REVIEW SHEET

... d. Give an example of this ratio (make one up)_________________________________ e. Name the 3 types of lipids_______________________________________________ f. At room temperature fats are______________________________________ g. At room temperature lipids are _______________________________________ ...
The Body`s Essential Building Blocks, Article by Gloria Gilbère, N.D.
The Body`s Essential Building Blocks, Article by Gloria Gilbère, N.D.

... proper pH balance in the gastrointestinal tract and body tissues. Scientific research confirms that proAUTHORS NOTE biotics are a vital necessity in the supThose that follow my work and writings know port of overall human health, that I credit goat-milk products for a major role in my specifically d ...
OVERALL MECHANISMS OF QUINOLONE RESISTANCE
OVERALL MECHANISMS OF QUINOLONE RESISTANCE

... Strains NalR -E.coli ...
Proteins in body fluids
Proteins in body fluids

... acid cycle, the cycles are interconnected in process dubbed the “Krebs bicycle”.  Each cycle can operate independently and connection between them depends on the transport of the intermediates between the mitochondrion and cytosol.  Aspartate formed in mitochondria by transamination between oxaloa ...
Fen-1 Nuclease in Genome Stability
Fen-1 Nuclease in Genome Stability

... One type of genomic instability results from the expansion of variable nucleotide repeat sequences, an excess of which cause of genetic diseases like Huntington’s disease, myotonic dystrophy, and fragile X syndrome. The expansion of trinucleotide repeats in certain regions of the human genome are mo ...
< 1 ... 183 184 185 186 187 188 189 190 191 ... 221 >

Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report