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Modelling interactomes
Modelling interactomes

... ASSOCIATE PROFESSOR UNIVERSITY OF WASHINGTON ...
Alkaline Phosphatase
Alkaline Phosphatase

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... C - General Characters of Arthropods: • All arthropods, while varying considerably in size and shape, have certain features in common. They are all bilaterally symmetrical and metamerically segmented. They have externally a hard chitinous exoskeleton, sometimes sclerotized or calcified, inside whic ...
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Dark induction and subcellular localization of the pathogenesis

... Antibodies raised against a PRB-lb-glutathione S-transferase fused protein cross-react with two proteins of 16 kDa and 20 kDa molecular mass, which are present in total extracts of ethyleneinduced tobacco leaves (Fig. 2A) [ 17]. These two proteins, identified by anti-PRB-lb antibodies, may result fr ...
Viral Vector Registration Form
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Bioinformatics Take Home Test #1 –Due 9/19/16

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... SNPs3D, PMut and SNPs&GO. The algorithm SIFT (6) is based on the principles of protein evolution, and uses sequence homology to predict the degree of amino acid conservation in protein sequences which correlates with the extent to which an amino acid substitution is tolerated (i.e. substitution is u ...
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lac Operon - Mediatech, Inc.

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Downstream Processes - Biological Engineering

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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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