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File - Georgetown ISD
File - Georgetown ISD

... • Structural genes- genes that are related and used in a biochemical pathway. • Promoter-The nucleotide sequence that can bind with RNA polymerase to start transcription. This sequence also contains the operator region. • Operator-The nucleotide sequence that can bind with repressor protein to inhib ...
Gene Regulation Prokaryoperon_RD_MP
Gene Regulation Prokaryoperon_RD_MP

... • Structural genes- genes that are related and used in a biochemical pathway. • Promoter-The nucleotide sequence that can bind with RNA polymerase to start transcription. This sequence also contains the operator region. • Operator-The nucleotide sequence that can bind with repressor protein to inhib ...
Translation and Protiens
Translation and Protiens

... Amino acids are linked together by peptide bonds to form one or more macromolecule subunits called polypeptides. Long chains of polypeptides result in the formation of proteins. The primary amimo acid sequence of a protein determines its secondary, tertiary, and quaternary structure, which then in t ...
TDH - an Enzyme Involved in Metabolising Threonine to Glycine
TDH - an Enzyme Involved in Metabolising Threonine to Glycine

... The preliminary structure of L-threonine dehydrogenase has been solved to 2.4Å by X-ray crystallography, which should allow us to hypothesise how this enzyme functions as a biological catalyst at the molecular level. ...
Human Growth Hormone
Human Growth Hormone

... Obtained from human pituitary gland only Urgent need to obtain human pituitary glands at autopsy High cost ...
Answers-to-exam-in-protein-chemistry-20130315-
Answers-to-exam-in-protein-chemistry-20130315-

... transition state (TS(IF); φ is only about 50-60% before). e) The results for the hydrophobic core mutation F20L provide further evidence that the intermediate I has a non-native hydrophobic core, which must be broken up in TS(IF) to allow formation of the native core (φ drops from 73 to 45 % when go ...
Prediction of Protein Structure Using Backbone Fragment
Prediction of Protein Structure Using Backbone Fragment

... protein sequence with a template structure or (ii) ab initio prediction methods. These methods suffer from the disadvantages of (a) lack of homologous template structure for a majority of new sequences or (b) untractably large conformational search space for ab initio predictions. We propose a metho ...
Ti (ID) - Educational Assistance
Ti (ID) - Educational Assistance

... traces to the Dictyo genome. A. Here is a list of “traces” that correspond to previously known sequences of Physarum. There are of two types: either a definite sequence is being re-sequenced and a close to 100% homology at the nucleotide level is observed or it is another member of a gene family tha ...
Evaluation and Comparison of the GUS, LUC and GFP Reporter
Evaluation and Comparison of the GUS, LUC and GFP Reporter

... Whether reporter activity is detectable within a plant cell or tissue depends on the promoter strength, duration of promoter activity, stability of the reporter mRNA, stability and activity of the reporter protein, the intrinsic background level within a tissue, and reporter signal detection techniq ...
of proteins
of proteins

... A typical example of a quaternary structure of the protein is hemoglobin or the protein that is responsible to carry oxygen, contained in our red blood cells. It consists of two different types of peptide chains (alpha and beta). Furthermore, each chain contains in its interior a non-protein molecu ...
The Leucine Binding Fluorescence Analysis of the Leucine Specific
The Leucine Binding Fluorescence Analysis of the Leucine Specific

... periplasmic substrate binding proteins act as initial receptors for transport, chemotaxis, antibiotic resistance, and energy utilization. There are more than 40 proteins that are classified as periplasmic binding proteins. The protein binds to the solute within the periplasm and mediates its transfe ...
Unfolded Protein Response (UPR)
Unfolded Protein Response (UPR)

... Looking for transcription factors which bind to BiP promoter Nucleotide sequence of the 2140 to 219 region of the human GRP78 promoter. ...
Use of Bioinformatic Databases
Use of Bioinformatic Databases

... TRANSFAC - database of eukaryotic cis-acting regulatory DNA elements and trans-acting factors EPD - eukaryotic promoter database DBTSS - DataBase of Transcriptional Start Sites (human) SCPD - Saccharomyces cerevisiae promoter database DCPD - Drosophila Core Promoter Database RegulonDB - a database o ...
EE 400: Practice using NCBI, Blast and Clustal
EE 400: Practice using NCBI, Blast and Clustal

... B. You will see a big box for “Input sequences.” Go to your Word document with all the sequences, and copy them all from the first > to the last amino acid. Paste this into the box. The format we have been using is called fastA, which is accepted by this program. Make sure you are not putting any bl ...
Slide 1
Slide 1

... The same name can be used to describe different concepts ...
protein expression after nacl treatment in two tomato cultivars
protein expression after nacl treatment in two tomato cultivars

... degradation can be used as an indicator of a plant's tolerance threshold after which the plant needs to provide essential amino acids to maintain protein synthesis at stressed sites (Feller et al., 2008). The second protein in band A was identified as a structural maintenance of chromosomes (SMC) pr ...
Gene Section RPS27 (ribosomal protein S27) Atlas of Genetics and Cytogenetics
Gene Section RPS27 (ribosomal protein S27) Atlas of Genetics and Cytogenetics

... offered arises from the relationship between RPS27, MDM2 and p53: RPS27 is a p53 repressible protein (He and Sun, 2007; Li et al., 2007). A 2011 study found that it competes with p53 for a central acidic binding domain on MDM2. Once bound, MDM2 is stimulated to ubiquinate and degrade the RPS27 or p5 ...
Gene expression profiling during conjugation of the Tetrahymena
Gene expression profiling during conjugation of the Tetrahymena

... types pair, go through meiosis, and exchange haploid nuclei. After postzygotic divisions and other developmental events, the cell is left with one micronucleus (MIC) and one macronucleus (MAC) (Miao et al., 2009). Much research has been devoted to this process, and there is evidence that a cyclin, ...
Virus to the rescue
Virus to the rescue

... pathogenic, biofilm-forming bacterium. Chem. Biol. 14, 387–398 (2007). ...
Proteome - Nematode bioinformatics. Analysis tools and data
Proteome - Nematode bioinformatics. Analysis tools and data

... linkage and many more… ...
Membrane Protein Expression in Cell
Membrane Protein Expression in Cell

... continuous development of new applications significantly promotes the distribution of this technology. Membrane protein synthesis by cell-free expression does not appear to be restricted by origin, size or topology of the target, and its global application is therefore a highly valuable characterist ...
Microbiology 13/14
Microbiology 13/14

... microbial genetics, molecular biology and biotechnology. Topics will include the life cycle of haploid and diploid cells of the common bakers’ yeast Saccharomyces cerevisiae, mating between haploid cells and the ability of yeast strains to carry out fermentation of sugars to alcohol. Students will a ...
PROTEIN SUBCELLULAR LOCALIZATION - PolyU
PROTEIN SUBCELLULAR LOCALIZATION - PolyU

... Identifying the functions of proteins is one of the fundamental targets in proteomics research. The subcellular locations of proteins can have significant influence on their functional characteristics. However, determination of subcellular localization entirely by laboratory tests is both time-consu ...
Building Blocks of Bodybuilding
Building Blocks of Bodybuilding

... 1. Low GI foods most of the time 2. High GI foods – On waking – After workout ...
ProBasics™
ProBasics™

... *These statements have not been evaluated by the Food & Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease. For educational purposes only. Consult your physician for any health problems. ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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