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Chapter 1 Introduction
Chapter 1 Introduction

... window opening to the world of life science. Thus, the knowledge of biochemistry which involves the study of chemical molecules and reactions in living organisms, and the elucidations of the nature of live phenomenon on the molecular level, is essential to medical students, as well as to the student ...
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... and simulation of the production of proteins. The first conclusion is that dynamic processes of molecular and biological systems in general, the protein production process in particular can be modeled as a discrete dynamic system. Two areas can benefit from such a methodology that has been presented ...
Psi-blast - Webcourse
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Follow Monty Python's Footsteps: Towards the Holy Grail of Protein Structure Prediction
Follow Monty Python's Footsteps: Towards the Holy Grail of Protein Structure Prediction

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Ruboyianes - University of Arizona
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... coat protein F synthesis was determined by comparing its band intensity and area with that of a host cell protein. The level of coat protein synthesis in amber H infections and in cells expressing the inhibitory protein was found to be approximately 20% of that of the wild-type control. The ⌬H prote ...
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... I201T, predicted by comparative modeling of the N-terminal part of bestrophin-1. Both analyzed mutations are substitutions replacing a highly evolutionary conserved amino acid with a much bulkier residue (cmr2) of different biochemical properties (cmr2, I201T). Such dramatic changes often affect str ...
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Recognition of Metal Ion Binding Proteins

... Since any classifier capable of identifying metalloproteins would also have to distinguish them from the neighboring non-metalloproteins, it is obvious that both the training set and the test set has to contain a set of metalloproteins and its nearest non-metal-binding neighbors in it. Furthermore s ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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