Download Synthetic Biology, Part A, Vol 497. Methods in Enzymology Brochure

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Synthetic Biology, Part A, Vol 497. Methods in Enzymology
Description:
Synthetic biology encompasses a variety of different approaches, methodologies and disciplines, and many
different definitions exist. This Volume of Methods in Enzymology has been split into 2 Parts and covers
topics such as Measuring and Engineering Central Dogma Processes, Mathematical and Computational
Methods and Next-Generation DNA Assembly and Manipulation.
- Encompasses a variety of different approaches, methodologies and disciplines
- Split into 2 parts and covers topics such as measuring and engineering central dogma processes,
mathematical and computational methods and next-generation DNA assembly and manipulation
Contents:
Section I. Measuring and Engineering Central Dogma Processes
Sequence-specificity and Energy Landscapes of DNA-binding Molecules
Joshua R. Tietjen, Leslie J. Donato, Devesh Bhimisaria and Aseem Z. Ansari
Promoter Reliability in Modular Transcriptional Networks
Rajat Anand, Navneet Rai and Mukund Thattai
The Analysis of ChIP-seq Data
Wenxiu Ma and Wing Hung Wong
Using DNA Microarrays to Assay Part Function
Virgil A. Rhodius and Carol A. Gross
Orthogonal gene expression in Escherichia coli
Wenlin An and Jason Chin
Directed evolution of promoters and tandem gene arrays for customizing RNA synthesis rates and
regulation
Keith E.J. Tyo, Elke Nevoigt and Gregory Stephanopoulos
Section II. Device and System Design, Optimization, and Debugging
Design and Connection of Robust Genetic Circuits
Ron Weiss
Engineering RNAi circuits
Yaakov Benenson
From SELEX to cell: Dual selections for synthetic riboswitches
Joy Sinha, Shana Topp, and Justin P. Gallivan
Using noisy gene expression mediated by engineered adenovirus to probe signaling dynamics in
mammalian cells
Jeffrey V. Wong, Guang Yao, Joseph R. Nevins and Lingchong You
De novo design and construction of an inducible gene expression system in mammalian cells
Maria Karlsson, Wilfried Weber and Martin Fussenegger
BioBuilding: Using banana-scented bacteria to teach synthetic biology
James Dixon and Natalie Kuldell
Section III. Device Measurement, Optimization, and Debugging
Use of Fluorescence Microscopy to Analyze Genetic Circuit Dynamics
Gürol Süel
Microfluidics for synthetic biology: From design to execution
M. S. Ferry, I. A. Razinkov, J. Hasty
Plate-based assays for light-regulated gene expression systems
Jeffrey J. Tabor
Spatiotemporal control of small GTPases with light using the LOV domain
Yi I. Wu, Xiaobo Wang, Li He, Denise Montell and Klaus M. Hahn
Light Control of Plasma Membrane Recruitment using the Phy-PIF system
Jared E. Toettcher, Delquin Gong, Wendell A. Lim, Orion D. Weiner
Synthetic Physiology: Strategies for Adapting Tools from Nature for Genetically-Targeted Control of Fast
Biological Processes
Ed Boyden
Section IV. Devices for Metabolic Engineering
Metabolic Pathway Flux Enhancement by Synthetic Protein Scaffolding
John E. Dueber and Weston R. Whitaker
A Synthetic Iterative Pathway for ketoacid elongation
C. R. Shen, J
C. Liao
Section V. Expanding Chasses
Synthetic Biology in Streptomyces bacteria
Marnix Medema, Rainer Breitling and Eriko Takano
Methods for Engineering Sulfate Reducing Bacteria of the Genus Desulfovibrio
Kimberly L. Keller, Judy D. Wall and Swapnil Chhabra
Modification of the Genome of Rhodobacter sphaeroides and Construction of Synthetic Operons
Paul R. Jaschke, Rafael G. Saer, Stephan Noll and J. Thomas Beatty
Synthetic Biology in Cyanobacteria: Engineering and Analyzing Novel Functions
Thorsten Heidorn, Daniel Camsund, Hsin-Ho Huang, Pia Lindberg, Paulo Oliveira, Karin Stensjö, and Peter
Lindblad
Developing a Synthetic Signal Transduction System in Plants
Kevin J. Morey, Mauricio S. Antunes, Kirk D. Albrecht, Tessa A. Bowen, Jared F. Troupe, Keira L. Havens and
June I. Medford
Lentiviral Vectors to Study Stochastic Noise in Gene Expression
Kate Franz, Abhyudai Singh, and Leor S. Weinberger
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