Supplementary Materials and Methods
... TTCAGACCATCCCAGAAACACC (+538). The number after the primer sequence corresponds to nucleotide position of 5-end of the primer relative to the transcription start site. PCR products were cloned into the pGL3-basic vector (Promega, Madison, WI) upstream of the firefly luciferase reporter gene. ...
... TTCAGACCATCCCAGAAACACC (+538). The number after the primer sequence corresponds to nucleotide position of 5-end of the primer relative to the transcription start site. PCR products were cloned into the pGL3-basic vector (Promega, Madison, WI) upstream of the firefly luciferase reporter gene. ...
III :
... (i) To have access to the Question Booklet, tear off the paper seal on the edge of this cover page. Do not accept a booklet without sticker-seal and do not accept an open booklet. (ii) Tally the number of pages and number of questions in the booklet with the information printed on the cover page. Fa ...
... (i) To have access to the Question Booklet, tear off the paper seal on the edge of this cover page. Do not accept a booklet without sticker-seal and do not accept an open booklet. (ii) Tally the number of pages and number of questions in the booklet with the information printed on the cover page. Fa ...
Novel Genetic Strategies for Cystinosis
... genome editing by creation of double-stranded breaks in DNA and subsequent repair by endogenous DNA repair machinery. Repair can occur via either non-homologous end joining (NHEJ), which involves error-prone repair without a template, or homology-directed repair (HDR), in which a donor template with ...
... genome editing by creation of double-stranded breaks in DNA and subsequent repair by endogenous DNA repair machinery. Repair can occur via either non-homologous end joining (NHEJ), which involves error-prone repair without a template, or homology-directed repair (HDR), in which a donor template with ...
Structures and Functions of Living Organisms (Lessons 1, 2, 5, 6, 8
... weak hydrogen bonds. The sequence of nucleotides in DNA codes for proteins, which is central key to cell function and life. Replication occurs during the S phase of the cell cycle and allows daughter cells to have an exact copy of parental DNA. Cells respond to their environments by producing ...
... weak hydrogen bonds. The sequence of nucleotides in DNA codes for proteins, which is central key to cell function and life. Replication occurs during the S phase of the cell cycle and allows daughter cells to have an exact copy of parental DNA. Cells respond to their environments by producing ...
Principles of sorting and assembly of peroxisomal alcohol
... Living cells are composed of several classes of biomolecules that are involved in the formation, function and maintenance of the cells. Two very important biomolecules are DNA (deoxyribonucleic acid) and proteins, which are equally important as one assures the existence of the other. DNA carries the ...
... Living cells are composed of several classes of biomolecules that are involved in the formation, function and maintenance of the cells. Two very important biomolecules are DNA (deoxyribonucleic acid) and proteins, which are equally important as one assures the existence of the other. DNA carries the ...
2005-06_AnnotCamp_IntroGO_panel1
... 1733 (title) A Brief Scheme of Ontology or the Science of Being in General. a1832 BENTHAM Fragm. Ontol. Wks. 1843 VIII. 195 The field of ontology, or as it may otherwise be termed, the field of supremely abstract entities, is a yet untrodden labyrinth. 1884 BOSANQUET tr. Lotze's Metaph. 22 Ontology. ...
... 1733 (title) A Brief Scheme of Ontology or the Science of Being in General. a1832 BENTHAM Fragm. Ontol. Wks. 1843 VIII. 195 The field of ontology, or as it may otherwise be termed, the field of supremely abstract entities, is a yet untrodden labyrinth. 1884 BOSANQUET tr. Lotze's Metaph. 22 Ontology. ...
Slide 1
... Blaschke and Valencia (2001) - 7 out of 19 unique interactions were present in the abstract Friedman et al (2001) ...
... Blaschke and Valencia (2001) - 7 out of 19 unique interactions were present in the abstract Friedman et al (2001) ...
Lecture notes: Genetics a.p.
... RNA splicing: RNA processing that removes introns and joins exons from eukaryotic premRNA; produces mature mRNA that will move into the cytoplasm from the nucleus. This is a “cut and paste” job. Pre-mRNA splicing is carried out by small nuclear ribonucleoproteins (snRNPs). Several snRNPs join with a ...
... RNA splicing: RNA processing that removes introns and joins exons from eukaryotic premRNA; produces mature mRNA that will move into the cytoplasm from the nucleus. This is a “cut and paste” job. Pre-mRNA splicing is carried out by small nuclear ribonucleoproteins (snRNPs). Several snRNPs join with a ...
Moving magnesium in plant cells - DigitalCommons@University of
... (EST) databases by homology to yeast MRS2. Arabidopsis MRS2-1 functionally complemented the yeast mrs2 mutant (Schock et al., 2000). A complementation screen of the yeast alr1⁄alr2 Mg uptake mutant strain identified MGT10 (also called MRS2-11) as a high affinity Mg transporter (Li et al., 2001), whi ...
... (EST) databases by homology to yeast MRS2. Arabidopsis MRS2-1 functionally complemented the yeast mrs2 mutant (Schock et al., 2000). A complementation screen of the yeast alr1⁄alr2 Mg uptake mutant strain identified MGT10 (also called MRS2-11) as a high affinity Mg transporter (Li et al., 2001), whi ...
Biol120 Mock Final Examination (v2.0)
... Questions and answers for this mock exam will be posted after today’s event http://www.usask.ca/ulc/sss, ...
... Questions and answers for this mock exam will be posted after today’s event http://www.usask.ca/ulc/sss, ...
Exam 3 Key
... a. the lagging strand has more short segments than the leading strand and those short segments are joined together by DNA ligase * b. DNA in the lagging strand is synthesized in the 3’----5’ direction c. the lagging strand has no RNA primase activity, it is replaced by the DNA ligase d. the lagging ...
... a. the lagging strand has more short segments than the leading strand and those short segments are joined together by DNA ligase * b. DNA in the lagging strand is synthesized in the 3’----5’ direction c. the lagging strand has no RNA primase activity, it is replaced by the DNA ligase d. the lagging ...
biochem ch 44B [9-2
... Developing progenitor cells in marrow grow in proximity with marrow stromal cells (fibroblasts, endothelial cells, adipocytes, and macrophages) – stromal cells form ECM and secrete growth factors that regulate hematopoietic development o Individual growth factor may stimulate proliferation, differ ...
... Developing progenitor cells in marrow grow in proximity with marrow stromal cells (fibroblasts, endothelial cells, adipocytes, and macrophages) – stromal cells form ECM and secrete growth factors that regulate hematopoietic development o Individual growth factor may stimulate proliferation, differ ...
Effective treatment of extensive and recalcitrant plantar warts
... ease of use, side effects, cosmesis, compliance, and patient preference. One of the most common first-line therapeutic methods, for instance, is cryosurgery as it is relatively safe and easily available. Despite of wide acceptance, the clearance rate for plantar warts is only 50% after executing ever ...
... ease of use, side effects, cosmesis, compliance, and patient preference. One of the most common first-line therapeutic methods, for instance, is cryosurgery as it is relatively safe and easily available. Despite of wide acceptance, the clearance rate for plantar warts is only 50% after executing ever ...
12A.H
... restriction enzymes. Restriction enzymes act a little like a knife or a pair of scissors. They cut up the DNA at specific points along the length of the DNA strand. Many different restriction enzymes exist, and they all have specific points where they cut the DNA. Human proteins like insulin can be ...
... restriction enzymes. Restriction enzymes act a little like a knife or a pair of scissors. They cut up the DNA at specific points along the length of the DNA strand. Many different restriction enzymes exist, and they all have specific points where they cut the DNA. Human proteins like insulin can be ...
Evolution of Metabolic Pathway
... ¾ Enzymes of the tyrosine biosynthesis pathway are encoded in almost as many complete genomes, with the exception of P. abyssi (live in 105ºC). ¾ A. aeolicus and four archaeal species encode 3dehydroquinate dehydratase and all the downstream enzymes but do not encode either DAHP synthase or 3-dehydr ...
... ¾ Enzymes of the tyrosine biosynthesis pathway are encoded in almost as many complete genomes, with the exception of P. abyssi (live in 105ºC). ¾ A. aeolicus and four archaeal species encode 3dehydroquinate dehydratase and all the downstream enzymes but do not encode either DAHP synthase or 3-dehydr ...
Teaching Cells and Things
... Directions: Read the following description about Domingo DNA. On a separate piece of paper, write a first name for each of the other characters listed below. Using the definitions in your book, write what each character does and what kind of personality they might have. Domingo DNA: Domingo DNA, or ...
... Directions: Read the following description about Domingo DNA. On a separate piece of paper, write a first name for each of the other characters listed below. Using the definitions in your book, write what each character does and what kind of personality they might have. Domingo DNA: Domingo DNA, or ...
Bacterial culture Microbiological cultures can be grown in petri
... including microorganisms, cells, large molecules, biopsy samples,metals, and crystals. Industrially, the electron microscope is often used for quality control and failure analysis. The electron microscope was invented and patented by Hungarian physicist Leó Szilárd who declined to construct it.[2] I ...
... including microorganisms, cells, large molecules, biopsy samples,metals, and crystals. Industrially, the electron microscope is often used for quality control and failure analysis. The electron microscope was invented and patented by Hungarian physicist Leó Szilárd who declined to construct it.[2] I ...
Lecture 1 Introduction to Micorarrays and Concepts of Molecular
... • Genes with similar expression patterns over all samples – We can compare the expression patterns ei and ei’ of two genes i and i' over all samples. • If we use cluster analysis, we can separate the genes into groups of genes with similar expression patterns (trees). • This will allow us to find wh ...
... • Genes with similar expression patterns over all samples – We can compare the expression patterns ei and ei’ of two genes i and i' over all samples. • If we use cluster analysis, we can separate the genes into groups of genes with similar expression patterns (trees). • This will allow us to find wh ...
doc
... E. Will ALWAYS have detectable levels of homology 5. What might be the reason for proteins that show significant similarity in their primary sequence (when no simple repeats or low complexity regions are present) to be homologous. A. Sequence space is so big that stumbling onto a significantly simil ...
... E. Will ALWAYS have detectable levels of homology 5. What might be the reason for proteins that show significant similarity in their primary sequence (when no simple repeats or low complexity regions are present) to be homologous. A. Sequence space is so big that stumbling onto a significantly simil ...
Slide
... between members of a population are neutral (or close to neutral). These differences can be fixed in the population through random genetic drift. Some mutations are strongly counter selected (this is why there are patterns of conserved residues). Only very seldom is a mutation under positive selecti ...
... between members of a population are neutral (or close to neutral). These differences can be fixed in the population through random genetic drift. Some mutations are strongly counter selected (this is why there are patterns of conserved residues). Only very seldom is a mutation under positive selecti ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.