E. coli
... low background incorporation due to its low level of endogenous mRNA. Wheat germ extracts translate RNA isolated from cells and tissue or those generated by in vitro transcription. When using RNA synthesized in vitro, the presence of a 5' cap structure may enhance translational activity. Typically, ...
... low background incorporation due to its low level of endogenous mRNA. Wheat germ extracts translate RNA isolated from cells and tissue or those generated by in vitro transcription. When using RNA synthesized in vitro, the presence of a 5' cap structure may enhance translational activity. Typically, ...
SUMMARY – Claire Munro Bananas (Musa sp.) serve as a staple
... worldwide. The crop, however, is vulnerable to several important diseases such as Fusarium wilt, caused by the soil-borne fungus Fusarium oxysporum f.sp. cubense (Foc). During the mid-20th century, thousands of acres of export banana plantations were lost in Central America due to Fusarium wilt. The ...
... worldwide. The crop, however, is vulnerable to several important diseases such as Fusarium wilt, caused by the soil-borne fungus Fusarium oxysporum f.sp. cubense (Foc). During the mid-20th century, thousands of acres of export banana plantations were lost in Central America due to Fusarium wilt. The ...
Regenerating Chicken Wings
... Salamanders and zebrafish can grow new limbs and fins, but chop off your own finger, and it's not going to grow back. Now researchers in San Diego have been able to regenerate wings in chicken embryos, which can't normally grow new limbs. The findings move scientists one step closer to understanding ...
... Salamanders and zebrafish can grow new limbs and fins, but chop off your own finger, and it's not going to grow back. Now researchers in San Diego have been able to regenerate wings in chicken embryos, which can't normally grow new limbs. The findings move scientists one step closer to understanding ...
Gene Section KLF5 (Kruppel-like factor 5 (intestinal)) Atlas of Genetics and Cytogenetics
... The KLF5 gene is deleted in about 33% prostate cancer cell lines/xenografts. Consistently, KLF5 mRNA is down-regulated in these samples compared to three immortalized prostate epithelial cell lines. In PC-3 prostate cancer cell line in which KLF5 mRNA is at normal high level, KLF5 protein is excessi ...
... The KLF5 gene is deleted in about 33% prostate cancer cell lines/xenografts. Consistently, KLF5 mRNA is down-regulated in these samples compared to three immortalized prostate epithelial cell lines. In PC-3 prostate cancer cell line in which KLF5 mRNA is at normal high level, KLF5 protein is excessi ...
1 Protein Synthesis DNA protein (nucleus) (ribosome) 1
... -so, a genetic code requires at least 20 different code words—one for each amino acid -when three nucleotides are grouped at a time, 64 triplet combinations are possible *provides enough code words, and some extra! (so more than one triplet can specify the same amino acid) -each nucleotide triplet i ...
... -so, a genetic code requires at least 20 different code words—one for each amino acid -when three nucleotides are grouped at a time, 64 triplet combinations are possible *provides enough code words, and some extra! (so more than one triplet can specify the same amino acid) -each nucleotide triplet i ...
1 Protein Synthesis DNA protein (nucleus) (ribosome) 1
... -so, a genetic code requires at least 20 different code words—one for each amino acid -when three nucleotides are grouped at a time, 64 triplet combinations are possible *provides enough code words, and some extra! (so more than one triplet can specify the same amino acid) -each nucleotide triplet i ...
... -so, a genetic code requires at least 20 different code words—one for each amino acid -when three nucleotides are grouped at a time, 64 triplet combinations are possible *provides enough code words, and some extra! (so more than one triplet can specify the same amino acid) -each nucleotide triplet i ...
SUPPLEMENTARY MATERIAL FOR:
... Table S3. The mRNA stem cell signature. 384 genes were found to be significantly enriched in stem cells in both transcriptomics platforms or significant in one and enriched >1.5-fold in the other. Table S4. Genes unique to the “Agilent stem cell signature”. Details for the 71 genes found to be uniqu ...
... Table S3. The mRNA stem cell signature. 384 genes were found to be significantly enriched in stem cells in both transcriptomics platforms or significant in one and enriched >1.5-fold in the other. Table S4. Genes unique to the “Agilent stem cell signature”. Details for the 71 genes found to be uniqu ...
MixMAP: An R Package for Mixed Modeling of Meta
... p value for each SNP as a measure of significance of the association with the trait. Since interest generally lies in characterizing association between genes (or gene regions) and the trait, where genes are comprised of multiple SNPs, an additional analysis step is required. One simple approach tha ...
... p value for each SNP as a measure of significance of the association with the trait. Since interest generally lies in characterizing association between genes (or gene regions) and the trait, where genes are comprised of multiple SNPs, an additional analysis step is required. One simple approach tha ...
1 Supplementary data Materials and methods Preparation of the
... DNA fragments encoding the entire mature sequence of human PDK3 (406 residues) were amplified by RT-PCR using RNA prepared from human skin fibroblast as template. The ...
... DNA fragments encoding the entire mature sequence of human PDK3 (406 residues) were amplified by RT-PCR using RNA prepared from human skin fibroblast as template. The ...
Avoiding Selective Pressure: Using Genomics to Design Anti-Virulence Drugs
... identified through DNA sequencing via similarity to characterized proteins.15 Other sequence-based clues can also be used in functional analysis; for example, computers could also be used to search for tandem repeats at the 5’ ends of genes; such repeats are often found in certain virulence genes.16 ...
... identified through DNA sequencing via similarity to characterized proteins.15 Other sequence-based clues can also be used in functional analysis; for example, computers could also be used to search for tandem repeats at the 5’ ends of genes; such repeats are often found in certain virulence genes.16 ...
Document
... orthologues or paralogues. Orthologues are similar sequences in two different organisms that have arisen due to a speciation event. Orthologs typically retain identical or similar functionality throughout evolution. Paralogues are similar sequences within a single organism that have arisen due t ...
... orthologues or paralogues. Orthologues are similar sequences in two different organisms that have arisen due to a speciation event. Orthologs typically retain identical or similar functionality throughout evolution. Paralogues are similar sequences within a single organism that have arisen due t ...
PDF
... greater-than symbol. RNA genes, including those of rRNA, tRNA, and other RNA genes, signal peptides longer than 100 bp (2,329,533– and long repeats are also indicated. Numbers on the tRNA symbols represent the number of tRNAs in 2,329,637 bp, 2,538,340–2,538,450 the cluster. bp, and 2,550,689–2,550, ...
... greater-than symbol. RNA genes, including those of rRNA, tRNA, and other RNA genes, signal peptides longer than 100 bp (2,329,533– and long repeats are also indicated. Numbers on the tRNA symbols represent the number of tRNAs in 2,329,637 bp, 2,538,340–2,538,450 the cluster. bp, and 2,550,689–2,550, ...
Why genes are regulated?
... each of them has a DNA binding region and a transcription-activating region. Binding ~22bp in a responsive promoter TGTGA conserved pentamer is essential and an inverted repeat version given the strongest interaction with CRP (increase affinity to DAN a lot) ...
... each of them has a DNA binding region and a transcription-activating region. Binding ~22bp in a responsive promoter TGTGA conserved pentamer is essential and an inverted repeat version given the strongest interaction with CRP (increase affinity to DAN a lot) ...
Biotechnology Lab (Kallas)
... contains four 0.5 x 1.0 cm microarrays each containing ~72,000 probes. Most of the ~3000 genes in the Synechococcus genome are covered with 7 probes repeated three times on each array. In addition there are ~6000 high-density “tiling” probes covering upstream regions of ~200 genes of interest for th ...
... contains four 0.5 x 1.0 cm microarrays each containing ~72,000 probes. Most of the ~3000 genes in the Synechococcus genome are covered with 7 probes repeated three times on each array. In addition there are ~6000 high-density “tiling” probes covering upstream regions of ~200 genes of interest for th ...
Matrixyl 3000 - Skin Beautiful Dermaceuticals
... Stimulation of gene expression Study to evaluate regulation of dermal and epidermal genes by matrikines present in MatrixylTM3000, using DNA-Array techniques on a reconstructed epidermis and a fibroblast culture. ...
... Stimulation of gene expression Study to evaluate regulation of dermal and epidermal genes by matrikines present in MatrixylTM3000, using DNA-Array techniques on a reconstructed epidermis and a fibroblast culture. ...
Mutations changes of genetic information
... disease ??? Increase of repetition number from one generation to the next (anticipation) Does not fit into our concept of classical genetics, molecular biology and mutations More repetitions, more severe disease Only in humans ??? Mostly in noncoding regions Also in exons, but only CAG = Glu ...
... disease ??? Increase of repetition number from one generation to the next (anticipation) Does not fit into our concept of classical genetics, molecular biology and mutations More repetitions, more severe disease Only in humans ??? Mostly in noncoding regions Also in exons, but only CAG = Glu ...
Handbook for Azospirillum
... Nester 1982) and pLA2917 (Fig. 4.2) (Allen and Hanson 1985). These low copy, broad-host range vectors, from the IncP1 group with RK2 replication factors, are not self-transmissible but can be mobilized if supplied with the plasmid transfer elements in trans (see Sect. 4.1.2). They can be transferred ...
... Nester 1982) and pLA2917 (Fig. 4.2) (Allen and Hanson 1985). These low copy, broad-host range vectors, from the IncP1 group with RK2 replication factors, are not self-transmissible but can be mobilized if supplied with the plasmid transfer elements in trans (see Sect. 4.1.2). They can be transferred ...
Maglott - Mouse Genome Informatics
... MGI:2448535) should be merged. This region of chr 5 has major assembly problems in the reference assembly, but the Celera assembly appears to accurately represent the structure as compared to transcript data and the orthologous regions of the human and rat genomes. In the reference assembly, Zfp469 ...
... MGI:2448535) should be merged. This region of chr 5 has major assembly problems in the reference assembly, but the Celera assembly appears to accurately represent the structure as compared to transcript data and the orthologous regions of the human and rat genomes. In the reference assembly, Zfp469 ...
hypothesize that AraC can exist in 2 states, P1 and P2
... -CAP is a symmetrical dimer of two identical subunits -when bound to cAMP (low glucose, high cAMP), CAP is active and binds to a specific palindrome found upstream of genes that are controlled by catabolite repression -consensus: 5’-AAATGTGATCT-AGATCACATTT-3’ -DNA binding mediated by a HTH present i ...
... -CAP is a symmetrical dimer of two identical subunits -when bound to cAMP (low glucose, high cAMP), CAP is active and binds to a specific palindrome found upstream of genes that are controlled by catabolite repression -consensus: 5’-AAATGTGATCT-AGATCACATTT-3’ -DNA binding mediated by a HTH present i ...
Basics of Gene regulation
... to take place, remodelling of the chromatin 2. Eukaryotic regulation: Eukaryotic cells have must occur wherein, acetylation of histone larger and more complex multimeric regulatory proteins and demethylation of DNA occur, which proteins when compared to bacterial cells. The then favours transcriptio ...
... to take place, remodelling of the chromatin 2. Eukaryotic regulation: Eukaryotic cells have must occur wherein, acetylation of histone larger and more complex multimeric regulatory proteins and demethylation of DNA occur, which proteins when compared to bacterial cells. The then favours transcriptio ...
GENETICS 603 Exam 1, September 27, 2013 1. Which of the
... only one that can pair with CAA then this could be a lethal defect and if it is one of very few, the general slowdown of translation could be a major problem. (This assumes that the anti ...
... only one that can pair with CAA then this could be a lethal defect and if it is one of very few, the general slowdown of translation could be a major problem. (This assumes that the anti ...
Microbial Genetics Lecture PowerPoint
... Understanding how beta-lactamase gene is turned on/off, can help us to design a drug to disable that gene’s expression (turn off the gene), making penicillin-resistant strains of Staph again vulnerable to penicillin. ...
... Understanding how beta-lactamase gene is turned on/off, can help us to design a drug to disable that gene’s expression (turn off the gene), making penicillin-resistant strains of Staph again vulnerable to penicillin. ...
tnf-alpha stimulated activation of mmp
... Degeneration of the intervertebral disc (IVD) involves a shift in the balance between catabolic and anabolic processes leading to changes in tissue architecture and function[1]. TNFα is a proinflammatory cytokine expressed within the nucleus pulposus (NP) of degenerate non-herniated IVDs, whose expr ...
... Degeneration of the intervertebral disc (IVD) involves a shift in the balance between catabolic and anabolic processes leading to changes in tissue architecture and function[1]. TNFα is a proinflammatory cytokine expressed within the nucleus pulposus (NP) of degenerate non-herniated IVDs, whose expr ...
ABCA17P - BMC Molecular Biology
... exons that share >70% sequence homology with the ABCA17P exons indicated. The yellow box highlights the alternative exon 1b of the ABCA17P gene. The green box represents a common CpG island at the 5' end of both genes. A metric scale bar is shown. (B) Comparison of the human and mouse ABCA17 – ABCA3 ...
... exons that share >70% sequence homology with the ABCA17P exons indicated. The yellow box highlights the alternative exon 1b of the ABCA17P gene. The green box represents a common CpG island at the 5' end of both genes. A metric scale bar is shown. (B) Comparison of the human and mouse ABCA17 – ABCA3 ...
BioInformatics at FSU - whose job is it and why it needs
... There’s a bewildering assortment of different databases and ways to access and manipulate the information within them. The key is to learn how to use that information in the most efficient manner. For example: Given a novel genome sequence, find all genes and p-genes. I want to design "sequence cap ...
... There’s a bewildering assortment of different databases and ways to access and manipulate the information within them. The key is to learn how to use that information in the most efficient manner. For example: Given a novel genome sequence, find all genes and p-genes. I want to design "sequence cap ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.