GRASP-DNA: A Web Application to Screen Prokaryotic Genomes for
... The ability to control multiple genes at the transcriptional level often relies on the existence of short stretches of well-defined DNA sequences, to which regulatory proteins and transcription factors bind. In this article we present a freely accessible webbased application (GRASP-DNA), that can be ...
... The ability to control multiple genes at the transcriptional level often relies on the existence of short stretches of well-defined DNA sequences, to which regulatory proteins and transcription factors bind. In this article we present a freely accessible webbased application (GRASP-DNA), that can be ...
Polyclonal Antibodies to Lamins - Edinburgh Research and Innovation
... immunofluorescence applications. Lamins are membrane proteins that provide a structural framework for the nucleus and are also essential for maintaining normal cell functions, such as cell cycle control, DNA replication and chromatin organization. Mutations in the genes encoding nuclear Lamins a ...
... immunofluorescence applications. Lamins are membrane proteins that provide a structural framework for the nucleus and are also essential for maintaining normal cell functions, such as cell cycle control, DNA replication and chromatin organization. Mutations in the genes encoding nuclear Lamins a ...
No Slide Title
... 16 plants contain gcs4 7 plants contain gcs3 6 plants contain gsat1 • No obvious phenotype in non-induced ...
... 16 plants contain gcs4 7 plants contain gcs3 6 plants contain gsat1 • No obvious phenotype in non-induced ...
Chemistry-Biology Interface Symposium Frontiers at the
... in vivo biosensor. We expand upon earlier work by testing the enzymatic activity of ferritin when annealed to AuNPs, employing electrophoretic assays to study the stability of ferritin-like particle (FLP) systems, and functionalizing the FLP systems for organization into higher order assemblies. APP ...
... in vivo biosensor. We expand upon earlier work by testing the enzymatic activity of ferritin when annealed to AuNPs, employing electrophoretic assays to study the stability of ferritin-like particle (FLP) systems, and functionalizing the FLP systems for organization into higher order assemblies. APP ...
Identification of the Cystic Fibrosis Gene: Cloning and
... large, contiguous segment of DNA spanning at least four transcribed sequences from a region thought to contain the CF locus (7). These sequences were initially identified on the basis of their ability to detect conserved sequences in other animal species by DNA hybridization and were subsequently ch ...
... large, contiguous segment of DNA spanning at least four transcribed sequences from a region thought to contain the CF locus (7). These sequences were initially identified on the basis of their ability to detect conserved sequences in other animal species by DNA hybridization and were subsequently ch ...
The relationship between amino acid sequences and protein folds.
... •! this model is supported by by misfolding mutations associated with certain diseases such as cystic fibrosis. Model 2: !The Global Model" •! the entire sequence of the protein contributes equally to the fold. •! this model is supported by mutation studies that show most mutations at any position h ...
... •! this model is supported by by misfolding mutations associated with certain diseases such as cystic fibrosis. Model 2: !The Global Model" •! the entire sequence of the protein contributes equally to the fold. •! this model is supported by mutation studies that show most mutations at any position h ...
Cloning and nucleotide sequence of a gene upstream of the eaeA
... the gene encoding the unique OMP and that the positive reaction with mAb 4E8C12 was due to non-specific binding. The complete DNA sequence of the clone 6-F insert (798 bp) is shown in Fig. 1. This clone contained 110 bp of the 5’ region of the EHEC eaeA gene (encoding 36 N-terminal amino acids of th ...
... the gene encoding the unique OMP and that the positive reaction with mAb 4E8C12 was due to non-specific binding. The complete DNA sequence of the clone 6-F insert (798 bp) is shown in Fig. 1. This clone contained 110 bp of the 5’ region of the EHEC eaeA gene (encoding 36 N-terminal amino acids of th ...
Gene Section SLC5A8 (solute carrier family 5 member 8)
... and pyruvate are inhibitors of histone deacetylases and are known to promote differentiation in normal colon epithelial cells but selectively induce apoptosis in tumor cells. - Kidney: lactate transport; reabsorption of lactate from urine to blood. - Thyroid: unknown function. When first identified, ...
... and pyruvate are inhibitors of histone deacetylases and are known to promote differentiation in normal colon epithelial cells but selectively induce apoptosis in tumor cells. - Kidney: lactate transport; reabsorption of lactate from urine to blood. - Thyroid: unknown function. When first identified, ...
Basics of Protein Expression
... Transfer vector method: co-transfect expression plasmid with 2nd p plasmid containing g required q viral genes, g , co-transfected cells produce virus, which is then amplified ...
... Transfer vector method: co-transfect expression plasmid with 2nd p plasmid containing g required q viral genes, g , co-transfected cells produce virus, which is then amplified ...
How to Find a Specific Gene or Protein to Study
... The initial search option, which is presented in the header as a text box with a "Go" button, is a keyword search against the text of the data records. Thus, it suffers from the same limitations as all keyword searches, such as misspellings and synonyms. Most genes and gene products can be described ...
... The initial search option, which is presented in the header as a text box with a "Go" button, is a keyword search against the text of the data records. Thus, it suffers from the same limitations as all keyword searches, such as misspellings and synonyms. Most genes and gene products can be described ...
- Horizon Discovery
... NanoLuc® luciferase is a novel, small, bright luciferase reporter. It is one third the size and 100 times brighter than the most commonly used luciferase, firefly. These features enable the development of homogenous assays for primary and secondary screening, reporting on pathways via the endogenous ...
... NanoLuc® luciferase is a novel, small, bright luciferase reporter. It is one third the size and 100 times brighter than the most commonly used luciferase, firefly. These features enable the development of homogenous assays for primary and secondary screening, reporting on pathways via the endogenous ...
Cdc45: the missing RecJ ortholog in eukaryotes?
... In HMMer and HHPred searches, Cdc45 sequences were found to be most similar to RecJ sequences than to any other DHH family protein. Closer similarity between Cdc45 and RecJ than to any other DHH proteins implies that these proteins may be orthologous, having been derived from a single homolog in the ...
... In HMMer and HHPred searches, Cdc45 sequences were found to be most similar to RecJ sequences than to any other DHH family protein. Closer similarity between Cdc45 and RecJ than to any other DHH proteins implies that these proteins may be orthologous, having been derived from a single homolog in the ...
Complete sequence analysis of the genome of the bacterium
... The latter result supports the analysis of cell fractionation experiments which indicate that the membrane fraction contains ∼50% of the total proteins estimated by SDS-PAGE. About 8% of the genome is composed of repetitive DNA elements RepMP1, RepMP2/3, RepMP4 and RepMP5, while only 67 of all predi ...
... The latter result supports the analysis of cell fractionation experiments which indicate that the membrane fraction contains ∼50% of the total proteins estimated by SDS-PAGE. About 8% of the genome is composed of repetitive DNA elements RepMP1, RepMP2/3, RepMP4 and RepMP5, while only 67 of all predi ...
pharmaceutical effects on gene expresson Edited Tambellini
... X-Gal which leads to characteristic blue colony phenotype. • This technique allows for the quick and easy detection of successful gene integration into plasmid, without the need to individually test each colony. • White colonies = AMPr, LAC Z disrupted • Blue colonies = AMPr , and LAC-z intact ...
... X-Gal which leads to characteristic blue colony phenotype. • This technique allows for the quick and easy detection of successful gene integration into plasmid, without the need to individually test each colony. • White colonies = AMPr, LAC Z disrupted • Blue colonies = AMPr , and LAC-z intact ...
Problem Set Four
... Q2. A strain of E. coli contains a Tn10 insertion within the mogA gene. This transposon carries a gene that confers resistance to tetracycline (Tetr). Two other genes, yaaH and htgA, are located near mogA and encode protein products that are easily selected and/or identified by biochemical assay. Th ...
... Q2. A strain of E. coli contains a Tn10 insertion within the mogA gene. This transposon carries a gene that confers resistance to tetracycline (Tetr). Two other genes, yaaH and htgA, are located near mogA and encode protein products that are easily selected and/or identified by biochemical assay. Th ...
HUMANIZATION N-GLYCOSYLATION PATHWAY PLANTS PLANT
... parts which do not encode amino acids (introns, lying between exons), as well as of so-called regulatory regions (mostly before or after the exon-intron-region). The latter are very important in the cell because they determine whether the information is just storaged without making use of it or whet ...
... parts which do not encode amino acids (introns, lying between exons), as well as of so-called regulatory regions (mostly before or after the exon-intron-region). The latter are very important in the cell because they determine whether the information is just storaged without making use of it or whet ...
A CRISPR design for next-generation antimicrobials Chase L Beisel
... how microbes evolve resistance. Remarkably, the survivors did not circumvent targeting - instead they either did not receive the CRISPR-Cas system, or they received a defective system, which is in line with previous findings [9]. The consistency of these findings would argue against the emergence of ...
... how microbes evolve resistance. Remarkably, the survivors did not circumvent targeting - instead they either did not receive the CRISPR-Cas system, or they received a defective system, which is in line with previous findings [9]. The consistency of these findings would argue against the emergence of ...
HL DNA_Jeopardy 2016
... And identify two things that would be not produced in low light intensity during the Light Dependent reaction that would affect the Calvin ...
... And identify two things that would be not produced in low light intensity during the Light Dependent reaction that would affect the Calvin ...
Characterization of Human Immunodeficiency Virus Type 1 from a
... FIG. 3. Predicted amino acid sequences of protease (A) and reverse transcriptase (B) of 13 drug-naive South African individuals. The sequences were aligned against a consensus generated from the sequences. There were no deletions or insertions. The dots indicate identical amino acids and changed ami ...
... FIG. 3. Predicted amino acid sequences of protease (A) and reverse transcriptase (B) of 13 drug-naive South African individuals. The sequences were aligned against a consensus generated from the sequences. There were no deletions or insertions. The dots indicate identical amino acids and changed ami ...
Facioscapulohumeral Muscular Dystrophy: The Molecular Signaling
... are adjacent to DUX4 [9,10]. FRG1 activation leads to increased activity of spliceosome. Also, with use of mouse model of FSHD, it was shown that FRG1 reduces the stability of mRNA of RBFOX1 [11], which plays an important role in alternative splicing - binds to exon-intron recognition motifs. Thus, ...
... are adjacent to DUX4 [9,10]. FRG1 activation leads to increased activity of spliceosome. Also, with use of mouse model of FSHD, it was shown that FRG1 reduces the stability of mRNA of RBFOX1 [11], which plays an important role in alternative splicing - binds to exon-intron recognition motifs. Thus, ...
Transient Overexpression of Recombinant Proteins in Mammalian
... In order to assess protein expression capabilities, both pHEK293 Ultra Expression Vector systems were used to introduce a fluorescent protein (AcGFP1) into adherent and suspension HEK 293 cells. For comparison, the same assay was conducted with a vector expressing AcGFP1 from a CMV promoter (pBApoC ...
... In order to assess protein expression capabilities, both pHEK293 Ultra Expression Vector systems were used to introduce a fluorescent protein (AcGFP1) into adherent and suspension HEK 293 cells. For comparison, the same assay was conducted with a vector expressing AcGFP1 from a CMV promoter (pBApoC ...
Functional dissection of the baculovirus late expression factor
... RNA polymerase complex, in order to define domains important for expression from a late viral promoter. Sitespecific mutagenesis analysis of the C-terminal motif sequence within LEF-8, which is part of the catalytic pocket of homologous polypeptides, revealed the importance of this motif in activity ...
... RNA polymerase complex, in order to define domains important for expression from a late viral promoter. Sitespecific mutagenesis analysis of the C-terminal motif sequence within LEF-8, which is part of the catalytic pocket of homologous polypeptides, revealed the importance of this motif in activity ...
Document
... *AUG s ign als tran slation initiation as w ell as codin g for Met ign als tran slation initiation as w ell as codin g for Met ...
... *AUG s ign als tran slation initiation as w ell as codin g for Met ign als tran slation initiation as w ell as codin g for Met ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.