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DNA Study guide
DNA Study guide

... 5. Know the role the various enzymes play in DNA replication. 6. How are mutations corrected? RNA and Transcription (section 8.4) 1. Know the three types of RNA and their functions. 2. Be able to explain the steps of transcription. 3. Know the role the various enzymes play in RNA transcription. 4. K ...
sanguinetti
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Lecture 8 (2/15/10) "DNA Forensics, Cancer, and Sequencing"
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... genetically susceptible to baldness. He was a palaeoEskimo, and by comparing his genome to other living people, they deduced that he was member of the Arctic Saqqaq, the first known culture to settle in Greenland whose ancestors had trekked from Siberia around the Arctic circle in pursuit of game. C ...
Lect2 Genetics
Lect2 Genetics

... DNA polymerase can only extend 5’ to 3’. Leading strand is generated normally, lagging strand goes opposite way so is done in ‘Okazaki’ ...
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DNA cr.eu updated plg latest
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... • The structure of euchromatin is reminiscent of an unfolded set of beads along a string, where in those beads represent nucleosomes. • Nucleosomes consist of eight proteins known as histone with approximately 147 base pairs of DNA wound around them; in euchromatin, this wrapping is loose so that th ...
Ch. 13.4: DNA Applications
Ch. 13.4: DNA Applications

... 2. How goes gel electrophoresis work? 3. What is a DNA fingerprint? Why are DNA fingerprints unique to each person? ...
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Escherichia coli his2

... Figure 7.5. Zoo-blotting. The objective is to determine if a fragment of human DNA hybridizes to DNAs from related species. Samples of human, chimp, cow and rabbit DNAs are therefore prepared, restricted, and electrophoresed in an agarose gel. Southern hybridization is then carried out with a human ...
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DNA and Central Dogma Study Guide
DNA and Central Dogma Study Guide

... 6. a) Label the sugars, phosphates, and missing bases in the picture to the ...
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Introduction Biotechnology Recombinant DNA Genetic Engineering

...  Amino-acid sequence detection via hybridization with probes o Reverse transcriptase-polymerase chain reaction  cDNA synthesis from mRNA present at time of interest during metabolic pathway / developmental stages  PRC amplification using gene specific primers  Gel electrophoresis indicates prese ...
BIO 101: Transcription and Translation
BIO 101: Transcription and Translation

... been completely transcribed In eukaryotes, this is pre-mRNA and must be further processed ...
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Ch 16 Lac Operon

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6.5 - Institut für Philosophie (HU Berlin)
6.5 - Institut für Philosophie (HU Berlin)

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Transcription and Translation

... A purine nucleotide can lose its base It will not base pair normally It will probably lead to a transition type mutation after the next round of replication. • Cytosine can be deaminated to uracil which can then create a problem ...
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Genetic Technology

... sequence (restriction site)  Same sequence found on both strands, running antiparallel  Enzyme cuts phosphodiester bonds of strands  These restriction fragments are double stranded with single stranded ends (“sticky ends”) ...
Extra Genetics - MrsAllisonMagee
Extra Genetics - MrsAllisonMagee

... • Epigenetics refers to heritable changes in gene expression (genes turned on vs off) that does not involve changes to the underlying DNA sequence. • It’s a change in phenotype without a change in genotype. ...
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Transcription and the Central Dogma

... • RNA polymerase is processive; once enzyme attaches to DNA, it can copy >10,000 nucleotides without falling off. • In eukaryotes, there are 3 RNA polymerases: – One for rRNA – One for tRNAs and some rRNA – One for all mRNAs and some small RNAs (involved in RNA processing) ...
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Genes and How they work!

... • Most eukaryotes posses Introns, Prokaryotes mostly do not! • Eukaryote mRNA contain transcripts of one gene. Prokaryote mRNA transcripts of several genes. • mRNA of eukaryotes must exit nucleus before translation can take place ...
Molecular genetics and molecular evolution
Molecular genetics and molecular evolution

... The more distantly related two species are the more genetic differences (amino acid changes or nucleotide changes) that will have accumulated between them. So, the longer the time since the organisms diverged, the greater the number of differences in the nucleotide sequence of the gene, e.g., cytoch ...
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CENTRAL DOGMA AND GENE REGULATION
CENTRAL DOGMA AND GENE REGULATION

... GENE REGULATION: Determines when a protein is expressed (produced) in a cell. Some proteins are always expressed while others are expressed intermittently (inducible). The Lac Operon: This is an example of a inducible expression. For E. coli to metabolize lactose several proteins must be produced by ...
Goal 3: Learner will develop an understanding of the continuity of
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Biological ethics
Biological ethics

... • A paradigm is much more than a theory – It includes a strong belief in the truth of one or more theories and shared opinions as to what problems are important and unimportant – What techniques and research methods are useful ...
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Promoter (genetics)



In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.
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