Genome-Scale Modeling of the Protein Secretory Machinery
... travel in the oposite sense than COPII [74], that’s from the Golgi to the ER. COPI vesicules are responsible for keeping the ER membrane in balance and also return ER luminal or membrane resident proteins that have escaped from ER to the Golgi [63]. The problems in formulation of the COPII vesicules ...
... travel in the oposite sense than COPII [74], that’s from the Golgi to the ER. COPI vesicules are responsible for keeping the ER membrane in balance and also return ER luminal or membrane resident proteins that have escaped from ER to the Golgi [63]. The problems in formulation of the COPII vesicules ...
Influence of genomic G+ C content on average amino
... The amino-acid composition of 23 490 proteins from 59 bacterial species was analyzed as a function of genomic G + C content. Observed amino-acid frequencies were compared with those expected from a neutral model assuming the absence of selection on average protein composition. Integral membrane prot ...
... The amino-acid composition of 23 490 proteins from 59 bacterial species was analyzed as a function of genomic G + C content. Observed amino-acid frequencies were compared with those expected from a neutral model assuming the absence of selection on average protein composition. Integral membrane prot ...
Intrinsic Protein Disorder and Protein
... irregular-MoRF corresponding to the thyroid receptor associated protein 220 (TRAP220), the cell death protein GRIM and the Alzheimer’s disease amyloid A4 protein homolog, respectively. Figure 1 shows the interacting residues and binding sites of the three selected cases. Figure 1.a shows four import ...
... irregular-MoRF corresponding to the thyroid receptor associated protein 220 (TRAP220), the cell death protein GRIM and the Alzheimer’s disease amyloid A4 protein homolog, respectively. Figure 1 shows the interacting residues and binding sites of the three selected cases. Figure 1.a shows four import ...
Membrane Adaptation and Solute Uptake Systems
... to identify the transmembrane α-helices. Structures can also be compared with those of some other types of membrane proteins from E. coli that have been studied extensively. However, in relation to transport proteins in general, bacteriorhodopsin is unusual in that it has 7 transmembrane α-helices, ...
... to identify the transmembrane α-helices. Structures can also be compared with those of some other types of membrane proteins from E. coli that have been studied extensively. However, in relation to transport proteins in general, bacteriorhodopsin is unusual in that it has 7 transmembrane α-helices, ...
Protein Synthesis Activity
... decoded. There are tRNA molecules present in the cell's cytoplasm. On each tRNA molecule, there are three exposed nitrogenous bases (called an anticodon) that will pair with a codon on mRNA. An anticodon specifies for each of the cell's 20 amino acids. By matching the tRNA anticodon to the mRNA codo ...
... decoded. There are tRNA molecules present in the cell's cytoplasm. On each tRNA molecule, there are three exposed nitrogenous bases (called an anticodon) that will pair with a codon on mRNA. An anticodon specifies for each of the cell's 20 amino acids. By matching the tRNA anticodon to the mRNA codo ...
Towards an Analysis of the Rice Mitochondrial Proteome
... Purified rice (Oryza sativa) mitochondrial proteins have been arrayed by isoelectric focusing/polyacrylamide gel electrophoresis (PAGE), by blue-native (BN) PAGE, and by reverse-phase high-performance liquid chromatography (LC) separation (LC-mass spectrometry [MS]). From these protein arrays, we ha ...
... Purified rice (Oryza sativa) mitochondrial proteins have been arrayed by isoelectric focusing/polyacrylamide gel electrophoresis (PAGE), by blue-native (BN) PAGE, and by reverse-phase high-performance liquid chromatography (LC) separation (LC-mass spectrometry [MS]). From these protein arrays, we ha ...
The Characterization of the Olf-1/EBF
... The luciferase reporter activity was measured from an equivalent amount of protein lysate of each sample with a luciferase assay system (Promega) and Monolight 2010 luminometer (Analytical Luminescence Laboratory, San Diego, CA). The relative luciferase activity was calculated by the reference indic ...
... The luciferase reporter activity was measured from an equivalent amount of protein lysate of each sample with a luciferase assay system (Promega) and Monolight 2010 luminometer (Analytical Luminescence Laboratory, San Diego, CA). The relative luciferase activity was calculated by the reference indic ...
Bioinformatics: Network Analysis Comparative Network Analysis Luay Nakhleh, Rice University
... scheme to detect conserved protein clusters, which takes into account interaction insertion/deletion and protein duplication events The algorithm, MaWISh, identified conserved subnetworks in the PPI networks of human and mouse, as well as conserved sub-networks across S. cerevisiae, C. elegans, and ...
... scheme to detect conserved protein clusters, which takes into account interaction insertion/deletion and protein duplication events The algorithm, MaWISh, identified conserved subnetworks in the PPI networks of human and mouse, as well as conserved sub-networks across S. cerevisiae, C. elegans, and ...
Different subcellular locations of secretome components of
... accumulates at this location. The authors argued that, due to the existence of a smaller number of cell wall components in this part of the cell, it might be more suited for the accumulation of large proteins. ActA of the Gram-positive intracellular bacterial pathogen Listeria monocytogenes also spa ...
... accumulates at this location. The authors argued that, due to the existence of a smaller number of cell wall components in this part of the cell, it might be more suited for the accumulation of large proteins. ActA of the Gram-positive intracellular bacterial pathogen Listeria monocytogenes also spa ...
New Computational Tools Help Solve Puzzle of RNA Structure
... “With any protein or RNA, in order for people to really understand how it works, they have to know its structure and where the interactions are happening,” says Gardner, who plans to have his initial RNA folding program completed in 2009. Gutell says Gardner’s preliminary results have been promising ...
... “With any protein or RNA, in order for people to really understand how it works, they have to know its structure and where the interactions are happening,” says Gardner, who plans to have his initial RNA folding program completed in 2009. Gutell says Gardner’s preliminary results have been promising ...
Reddy, Kirthi: Analysis of Caenorhabditis elegans protein T09E8.2
... significant hits were found; also, no obvious biological relevance was seen amongst these significant hits. The statistically insignificant results found with these searches contained some potentially interesting proteins, such as DNA repair proteins and centromere proteins. Experimental results are ...
... significant hits were found; also, no obvious biological relevance was seen amongst these significant hits. The statistically insignificant results found with these searches contained some potentially interesting proteins, such as DNA repair proteins and centromere proteins. Experimental results are ...
Nuclear function for the actin-binding cytoskeletal protein
... In the last few years, it has been shown that, besides the nuclear intermedier filamentforming lamins, several cytoskeletal components are also present in the nucleus including actin, motor proteins and crosslinking proteins (Simon and Wilson 2011). However, the existence of a structure mechanically ...
... In the last few years, it has been shown that, besides the nuclear intermedier filamentforming lamins, several cytoskeletal components are also present in the nucleus including actin, motor proteins and crosslinking proteins (Simon and Wilson 2011). However, the existence of a structure mechanically ...
Characterization of interactions between LPS transport proteins of
... Gram-negative bacteria, such as Escherichia coli, where it is necessary to move lipids across two membrane bilayers to the cell exterior. This double membrane envelops the cytoplasm, protecting it from the external environment and acting as a selectively permeable barrier to compounds passing in and ...
... Gram-negative bacteria, such as Escherichia coli, where it is necessary to move lipids across two membrane bilayers to the cell exterior. This double membrane envelops the cytoplasm, protecting it from the external environment and acting as a selectively permeable barrier to compounds passing in and ...
STRUCTURE AND ALLERGENICITY OF WHEAT GLUTEN PROTEINS
... Because both of these protein fractions play a significant role in the human diet, intensive research has been carried out to explore the biochemical and molecular reasons for their allergenicity, and the possibilities for eliminating or limiting it. In spite of much progress, this problem has not b ...
... Because both of these protein fractions play a significant role in the human diet, intensive research has been carried out to explore the biochemical and molecular reasons for their allergenicity, and the possibilities for eliminating or limiting it. In spite of much progress, this problem has not b ...
How an Organism`s Genotype Determines Its Phenotype How an
... – studied strains of mold that were unable to grow on the usual growth medium, – determined that these strains lacked an enzyme in a metabolic pathway that synthesized arginine, – showed that each mutant was defective in a single gene, and – hypothesized that the function of an individual gene is to ...
... – studied strains of mold that were unable to grow on the usual growth medium, – determined that these strains lacked an enzyme in a metabolic pathway that synthesized arginine, – showed that each mutant was defective in a single gene, and – hypothesized that the function of an individual gene is to ...
The deleterious effect of missense mutations on pre
... are usually recognized by a family of proteins known as SR proteins, which contain an RNAbinding domain and a region rich in arginine-serine dipeptides (RS domain). It is likely that SR proteins are required for the correct splicing of most exons. However, regulation of premRNA splicing is much more ...
... are usually recognized by a family of proteins known as SR proteins, which contain an RNAbinding domain and a region rich in arginine-serine dipeptides (RS domain). It is likely that SR proteins are required for the correct splicing of most exons. However, regulation of premRNA splicing is much more ...
From Gene to Protein
... – Is either translated into an amino acid or serves as a translational stop signal ...
... – Is either translated into an amino acid or serves as a translational stop signal ...
Section D - Prokaryotic and Eukaryotic Chromosome Structure
... able to undergo more movement than the other two bases and can thus form non-standard base pairs as long as the distances between the ribose units are close to normal. ...
... able to undergo more movement than the other two bases and can thus form non-standard base pairs as long as the distances between the ribose units are close to normal. ...
Information Retrieval Performance and Method
... 16,120,074 abstracts (2007 release). Up to top 1000 abstracts were retrieved for each of the 560 AD query proteins, resulting in a collection of 222,609 unique PubMed abstracts—4 times the size of PubMed abstracts that were retrieved with the simple query term “Alzheimer” against the same database. ...
... 16,120,074 abstracts (2007 release). Up to top 1000 abstracts were retrieved for each of the 560 AD query proteins, resulting in a collection of 222,609 unique PubMed abstracts—4 times the size of PubMed abstracts that were retrieved with the simple query term “Alzheimer” against the same database. ...
The random character of protein evolution and its effect on the
... random effect is very large. It can then render trivial the statistical disadvantage inherent in using a crude measure of protein difference, such as amino acid composition or immunological cross-reactivity, in preference to a measure based on amino acid sequence. In some cases, such as classificati ...
... random effect is very large. It can then render trivial the statistical disadvantage inherent in using a crude measure of protein difference, such as amino acid composition or immunological cross-reactivity, in preference to a measure based on amino acid sequence. In some cases, such as classificati ...
CHAPTER 6
... stimulatory G protein is known as Gs and the inhibitory G protein is known as Gi • Gi may act either by the Gia subunit binding to AC or by the Gibg complex complexing all the Gsa and preventing it from binding to AC • Read about the actions of cholera toxin and pertussis toxin Garrett and Grisham, ...
... stimulatory G protein is known as Gs and the inhibitory G protein is known as Gi • Gi may act either by the Gia subunit binding to AC or by the Gibg complex complexing all the Gsa and preventing it from binding to AC • Read about the actions of cholera toxin and pertussis toxin Garrett and Grisham, ...
Presentation
... sequence of amino acids. The sequence determines secondary and tertiary structure—how the protein is folded. The number of different proteins that can be made from 20 amino acids is enormous! ...
... sequence of amino acids. The sequence determines secondary and tertiary structure—how the protein is folded. The number of different proteins that can be made from 20 amino acids is enormous! ...
Identification of cellular proteins that bind to the human
... MRC Retrovirus Research Laboratory, Department of Veterinary Pathology, University of Glasgow, Bearsden Road, Glasgow G61 1QH, U.K. ...
... MRC Retrovirus Research Laboratory, Department of Veterinary Pathology, University of Glasgow, Bearsden Road, Glasgow G61 1QH, U.K. ...
Practical Proteins and Amino Acids` Identification
... Biuret’s test Principle: Biuret test is Specific for Proteins – To differentiate between Proteins (+ve) and Amino Acids (-ve). The biuret reagent (copper sulfate in a strong base) reacts with peptide bonds in proteins to form a violet complex known as the “Biuret complex”. Two peptide bonds are at ...
... Biuret’s test Principle: Biuret test is Specific for Proteins – To differentiate between Proteins (+ve) and Amino Acids (-ve). The biuret reagent (copper sulfate in a strong base) reacts with peptide bonds in proteins to form a violet complex known as the “Biuret complex”. Two peptide bonds are at ...
EPIgeneousTM Binding Domain Assays
... with the substrate brings the donor and acceptor dyes into close proximity, enabling FRET to occur upon light excitation. The specific signal at 665 nm is inhibited when a specific compound prevents the reader domain protein from binding to its substrate. ...
... with the substrate brings the donor and acceptor dyes into close proximity, enabling FRET to occur upon light excitation. The specific signal at 665 nm is inhibited when a specific compound prevents the reader domain protein from binding to its substrate. ...
SR protein
SR proteins are a conserved family of proteins involved in RNA splicing. SR proteins are named because they contain a protein domain with long repeats of serine and arginine amino acid residues, whose standard abbreviations are ""S"" and ""R"" respectively. SR proteins are 50-300 amino acids in length and composed of two domains, the RNA recognition motif (RRM) region and the RS binding domain. SR proteins are more commonly found in the nucleus than the cytoplasm, but several SR proteins are known to shuttle between the nucleus and the cytoplasm.SR proteins were discovered in the 1990s in Drosophila and in amphibian oocytes, and later in humans. In general, metazoans appear to have SR proteins and unicellular organisms lack SR proteins.SR proteins are important in constitutive and alternative pre-mRNA splicing, mRNA export, genome stabilization, nonsense-mediated decay, and translation. SR proteins alternatively splice pre-mRNA by preferentially selecting different splice sites on the pre-mRNA strands to create multiple mRNA transcripts from one pre-mRNA transcript. Once splicing is complete the SR protein may or may not remain attached to help shuttle the mRNA strand out of the nucleus. As RNA Polymerase II is transcribing DNA into RNA, SR proteins attach to newly made pre-mRNA to prevent the pre-mRNA from binding to the coding DNA strand to increase genome stabilization. Topoisomerase I and SR proteins also interact to increase genome stabilization. SR proteins can control the concentrations of specific mRNA that is successfully translated into protein by selecting for nonsense-mediated decay codons during alternative splicing. SR proteins can alternatively splice NMD codons into its own mRNA transcript to auto-regulate the concentration of SR proteins. Through the mTOR pathway and interactions with polyribosomes, SR proteins can increase translation of mRNA.Ataxia telangiectasia, neurofibromatosis type 1, several cancers, HIV-1, and spinal muscular atrophy have all been linked to alternative splicing by SR proteins.