DNase I (AMPD1) - Technical Bulletin - Sigma
... DNase I has been purified to remove RNase activity, and is suitable for eliminating DNA from RNA preparations prior to sensitive applications, such as RTPCR (Reverse Transcriptase – Polymerase Chain Reaction). No current RNA isolation procedure removes 100% of the DNA. Because PCR can detect even a ...
... DNase I has been purified to remove RNase activity, and is suitable for eliminating DNA from RNA preparations prior to sensitive applications, such as RTPCR (Reverse Transcriptase – Polymerase Chain Reaction). No current RNA isolation procedure removes 100% of the DNA. Because PCR can detect even a ...
Full Text
... modification observed in plants, animals, fungi, and viruses; it is performed by attached myristic acid in proteins. Myristoylation can affect conformational stability of proteins by interaction with membranes or the hydrophobic domains of other proteins (Podell & Gribskov, 2004; Zheng et al., 1993; ...
... modification observed in plants, animals, fungi, and viruses; it is performed by attached myristic acid in proteins. Myristoylation can affect conformational stability of proteins by interaction with membranes or the hydrophobic domains of other proteins (Podell & Gribskov, 2004; Zheng et al., 1993; ...
Chen-6-Translation
... • Initiation-- once per protein it gets the system in motion • Elongation-- repeated for each codon in the mRNA making a peptide bond • Termination-- finishes and releases the newly synthesized protein ...
... • Initiation-- once per protein it gets the system in motion • Elongation-- repeated for each codon in the mRNA making a peptide bond • Termination-- finishes and releases the newly synthesized protein ...
Document
... • Inhibitors can block the active site • Inhibitors can pull on another part of the enzyme and stretch the active site out of shape • phosphates and other factors can pull on another part of the enzyme to pull the active site into the correct shape ...
... • Inhibitors can block the active site • Inhibitors can pull on another part of the enzyme and stretch the active site out of shape • phosphates and other factors can pull on another part of the enzyme to pull the active site into the correct shape ...
Biomolecules
... Amino acids contain amino (–NH2) and carboxyl (–COOH) functional groups. Depending upon the relative position of amino group with respect to carboxyl group, the amino acids can be R CH COOH classified as α, β, γ, δ and so on. Only α-amino acids are obtained on hydrolysis of proteins. They NH2 may co ...
... Amino acids contain amino (–NH2) and carboxyl (–COOH) functional groups. Depending upon the relative position of amino group with respect to carboxyl group, the amino acids can be R CH COOH classified as α, β, γ, δ and so on. Only α-amino acids are obtained on hydrolysis of proteins. They NH2 may co ...
Identification of Novel microRNA Regulatory Proteins in Neurons
... significant repression by miR-134. By doing so, we regulate the function of other dendritic miRNAs. could discriminate between effects that result from a The brain-enriched miR-138 is present at synaptic specific interaction with miR-134 and more general sites and functions, similar to miR-134, as a ...
... significant repression by miR-134. By doing so, we regulate the function of other dendritic miRNAs. could discriminate between effects that result from a The brain-enriched miR-138 is present at synaptic specific interaction with miR-134 and more general sites and functions, similar to miR-134, as a ...
Exploration of the Dynamic Properties of Protein Complexes
... shows that protein A interacts with B, B interacts with C and A does not interact with C (Figure 3A). One possible conformation is shown in Figure 3B with the constraints that are required to be satisfied (Figure 3C,D). A more detailed example is illustrated in Figure S1. A non-interacting pair prov ...
... shows that protein A interacts with B, B interacts with C and A does not interact with C (Figure 3A). One possible conformation is shown in Figure 3B with the constraints that are required to be satisfied (Figure 3C,D). A more detailed example is illustrated in Figure S1. A non-interacting pair prov ...
Expression and Purification of Toxoplasma gondii Cell Cycle
... Disease Control approximately 60 million people in the U.S. are currently infected with T. gondii. Treatments for toxoplasmosis are limited and generally consist of a combination of pharmaceuticals. Based on the prevalence and limited options for treatment new drug targets should be explored. Cell c ...
... Disease Control approximately 60 million people in the U.S. are currently infected with T. gondii. Treatments for toxoplasmosis are limited and generally consist of a combination of pharmaceuticals. Based on the prevalence and limited options for treatment new drug targets should be explored. Cell c ...
Document
... affinity for sites 3, 4, and 5 than for sites 3’, 4’, and 5’. -sites 3,4, and 5 are exactly 3-bps short of maximal spacing for promoting RNA polymerase binding. ...
... affinity for sites 3, 4, and 5 than for sites 3’, 4’, and 5’. -sites 3,4, and 5 are exactly 3-bps short of maximal spacing for promoting RNA polymerase binding. ...
revised
... Using the TMHMM program, a total of 21473 TMs for the mesophilic set of proteins and 13340 for the thermophilic organims were predicted. The average length of the predicted TMs was 22 for both sets of proteins (thermophilic and mesophilic), which is in good agreement with the average length of TMs p ...
... Using the TMHMM program, a total of 21473 TMs for the mesophilic set of proteins and 13340 for the thermophilic organims were predicted. The average length of the predicted TMs was 22 for both sets of proteins (thermophilic and mesophilic), which is in good agreement with the average length of TMs p ...
Nucleolin: A Multifunctional Major Nucleolar Phosphoprotein
... evolution. Nucleolin contains a specific bipartite nuclear localization signal sequence and possesses a number of unusual structural features. It has unique tripartite structure and each domain performs a specific function by interacting with DNA or RNA or proteins. Nucleolin exhibits intrinsic self ...
... evolution. Nucleolin contains a specific bipartite nuclear localization signal sequence and possesses a number of unusual structural features. It has unique tripartite structure and each domain performs a specific function by interacting with DNA or RNA or proteins. Nucleolin exhibits intrinsic self ...
Probing protein function by chemical modification
... Studying protein function in vitro or in the context of live cells and organisms is of vital importance in biological research. Genetic tags such as fluorescent proteins (FPs) are widely used to detect proteins. The 2008 Nobel Prize in Chemistry rewarded the discovery and use of GFP as a tagging too ...
... Studying protein function in vitro or in the context of live cells and organisms is of vital importance in biological research. Genetic tags such as fluorescent proteins (FPs) are widely used to detect proteins. The 2008 Nobel Prize in Chemistry rewarded the discovery and use of GFP as a tagging too ...
From The Building Blocks to Life
... RNA and it evolves through changes in these sequences. Protein enzymes catalyze most of the chemical reactions essential for life. The biological basic functions of information storage and catalysis are fulfilled by biopolymers, i.e. polymers made from repeating monomers, the building blocks of life ...
... RNA and it evolves through changes in these sequences. Protein enzymes catalyze most of the chemical reactions essential for life. The biological basic functions of information storage and catalysis are fulfilled by biopolymers, i.e. polymers made from repeating monomers, the building blocks of life ...
Different packing of external residues can explain differences in the
... (homologous thermophilic and mesophilic proteins have different structures, and the compactness of thermophilic protein is greater), and the other is concerned with essential modifications of amino acid sequences of proteins (homologous thermophilic and mesophilic proteins have similar structures, b ...
... (homologous thermophilic and mesophilic proteins have different structures, and the compactness of thermophilic protein is greater), and the other is concerned with essential modifications of amino acid sequences of proteins (homologous thermophilic and mesophilic proteins have similar structures, b ...
RNA
... intron or exon-exon junction spanning length: 9-30 bp (ideally: 20 bp) melting temperature Tm: 58-60 °C (ideally: 59°C) ...
... intron or exon-exon junction spanning length: 9-30 bp (ideally: 20 bp) melting temperature Tm: 58-60 °C (ideally: 59°C) ...
INTRODUCTION
... The opposite strand is called the coding strand or the sense strand as well as the nontemplate strand The base sequence is identical to the RNA transcript Except for the substitution of uracil in RNA for thymine in DNA ...
... The opposite strand is called the coding strand or the sense strand as well as the nontemplate strand The base sequence is identical to the RNA transcript Except for the substitution of uracil in RNA for thymine in DNA ...
A General Method Applicable to the Search for Similarities in the
... 6. Results and Discussion The use of a small random sample size (ten)was necessary to hold the computer time to a reasonable level. The maximum probable error in a standard deviation estimate for a sample this small is quite large and the resultsshould be judged with this fact in mind. For each set ...
... 6. Results and Discussion The use of a small random sample size (ten)was necessary to hold the computer time to a reasonable level. The maximum probable error in a standard deviation estimate for a sample this small is quite large and the resultsshould be judged with this fact in mind. For each set ...
Detonator of the population explosion
... hat is the most important invention of the twentieth century? Aeroplanes, nuclear energy, space flight, television and computers will be the most common answers. Yet none of these can match the synthesis of ammonia from its elements. The world might be better off without Microsoft and CNN, and neith ...
... hat is the most important invention of the twentieth century? Aeroplanes, nuclear energy, space flight, television and computers will be the most common answers. Yet none of these can match the synthesis of ammonia from its elements. The world might be better off without Microsoft and CNN, and neith ...
Functions of Ribosome-Associated Chaperones and their Interaction
... ribosomes with a KD of approximately 1 µM and a mean residence time of 10–15 s, the presence of a nascent chain increases the affinity of TF for ribosomes up to 30fold [20, 24–26]. It could be shown by cross-linking studies combined with cryoelectron microscopy structures that TF interacts with very ...
... ribosomes with a KD of approximately 1 µM and a mean residence time of 10–15 s, the presence of a nascent chain increases the affinity of TF for ribosomes up to 30fold [20, 24–26]. It could be shown by cross-linking studies combined with cryoelectron microscopy structures that TF interacts with very ...
Relationship between relative protein value and some in vitro in
... should therefore be a reasonable approach to determine the bioavailability of the AAs and hence the protein quality (Stahman and Woldegiorgis, 1975). In vivo studies in experimental animals (Chen et al., 1962) as well as in humans (Chung et al., 1976) have shown that following a protein meal., the c ...
... should therefore be a reasonable approach to determine the bioavailability of the AAs and hence the protein quality (Stahman and Woldegiorgis, 1975). In vivo studies in experimental animals (Chen et al., 1962) as well as in humans (Chung et al., 1976) have shown that following a protein meal., the c ...
Chapter 6 Protein: Amino Acids The Chemist`s View of Proteins
... Polypeptide chains twist depending on their amino acid sequence Protein functions-unique shape allows them to perform their function Protein denaturization-heat, acid disturb their stability and cause them to denature; they uncoil and lose their shape and function Protein Digestion in the GI Tract P ...
... Polypeptide chains twist depending on their amino acid sequence Protein functions-unique shape allows them to perform their function Protein denaturization-heat, acid disturb their stability and cause them to denature; they uncoil and lose their shape and function Protein Digestion in the GI Tract P ...
CHAPTER 5 Gene Expression: Transcription
... show different levels of transcription, corresponding with sigma’s ability to recognize their sequences. • 10. E. coli has several sigma factors with important roles in gene regulation. Each sigma can bind a molecule of core RNA polymerase and guide its choice of genes to transcribe. • 11. Most E. c ...
... show different levels of transcription, corresponding with sigma’s ability to recognize their sequences. • 10. E. coli has several sigma factors with important roles in gene regulation. Each sigma can bind a molecule of core RNA polymerase and guide its choice of genes to transcribe. • 11. Most E. c ...
RnaUs Total Viral RNA/DNA Prep
... LeGene RnaUs Total Viral RN A P r e p provides an easy and reliable method for isolating total viral RNA from plasma or serum while enzyme inhibitors and other contaminates completely removed. This procedure has been validated for isolating nucleic acids from Hepatitis A, Hepatitis B, Hepatitis C an ...
... LeGene RnaUs Total Viral RN A P r e p provides an easy and reliable method for isolating total viral RNA from plasma or serum while enzyme inhibitors and other contaminates completely removed. This procedure has been validated for isolating nucleic acids from Hepatitis A, Hepatitis B, Hepatitis C an ...
Degradation by bacteria of membrane and soluble protein in seawater
... degraded at significantly slower rates ('4 to 'lb) than the soluble proteins. Proteins determined to be intimately associated with the membrane were not degraded during the initial 45 h, while a substantial fraction of soluble proteins was degraded during the same period. The data are consistent wit ...
... degraded at significantly slower rates ('4 to 'lb) than the soluble proteins. Proteins determined to be intimately associated with the membrane were not degraded during the initial 45 h, while a substantial fraction of soluble proteins was degraded during the same period. The data are consistent wit ...
SR protein
SR proteins are a conserved family of proteins involved in RNA splicing. SR proteins are named because they contain a protein domain with long repeats of serine and arginine amino acid residues, whose standard abbreviations are ""S"" and ""R"" respectively. SR proteins are 50-300 amino acids in length and composed of two domains, the RNA recognition motif (RRM) region and the RS binding domain. SR proteins are more commonly found in the nucleus than the cytoplasm, but several SR proteins are known to shuttle between the nucleus and the cytoplasm.SR proteins were discovered in the 1990s in Drosophila and in amphibian oocytes, and later in humans. In general, metazoans appear to have SR proteins and unicellular organisms lack SR proteins.SR proteins are important in constitutive and alternative pre-mRNA splicing, mRNA export, genome stabilization, nonsense-mediated decay, and translation. SR proteins alternatively splice pre-mRNA by preferentially selecting different splice sites on the pre-mRNA strands to create multiple mRNA transcripts from one pre-mRNA transcript. Once splicing is complete the SR protein may or may not remain attached to help shuttle the mRNA strand out of the nucleus. As RNA Polymerase II is transcribing DNA into RNA, SR proteins attach to newly made pre-mRNA to prevent the pre-mRNA from binding to the coding DNA strand to increase genome stabilization. Topoisomerase I and SR proteins also interact to increase genome stabilization. SR proteins can control the concentrations of specific mRNA that is successfully translated into protein by selecting for nonsense-mediated decay codons during alternative splicing. SR proteins can alternatively splice NMD codons into its own mRNA transcript to auto-regulate the concentration of SR proteins. Through the mTOR pathway and interactions with polyribosomes, SR proteins can increase translation of mRNA.Ataxia telangiectasia, neurofibromatosis type 1, several cancers, HIV-1, and spinal muscular atrophy have all been linked to alternative splicing by SR proteins.