Vragen voor tentamen Protein Engineering (8S080)
... a. What is the classical method to obtain monoclonal antibodies?. Classical monoclonal antibodies are not very attractive for commercial application in shampoo, because these antibodies can only be produced in mammalian cell lines. Unilever therefore decides to use llamas to raise antibodies against ...
... a. What is the classical method to obtain monoclonal antibodies?. Classical monoclonal antibodies are not very attractive for commercial application in shampoo, because these antibodies can only be produced in mammalian cell lines. Unilever therefore decides to use llamas to raise antibodies against ...
PowerPoint bemutató
... ER, Golgi retrieved by the KDEL-receptors. They recognize the KDEL signal (Lys-Asp-Glu-Leu at C-terminus). ...
... ER, Golgi retrieved by the KDEL-receptors. They recognize the KDEL signal (Lys-Asp-Glu-Leu at C-terminus). ...
PowerPoint bemutató
... ER, Golgi retrieved by the KDEL-receptors. They recognize the KDEL signal (Lys-Asp-Glu-Leu at C-terminus). ...
... ER, Golgi retrieved by the KDEL-receptors. They recognize the KDEL signal (Lys-Asp-Glu-Leu at C-terminus). ...
Chapter 17: Gene Expression Gene Expression DNA houses all
... Introns (interrupt transcript) – long regions of noncoding RNA segments Exons (expressed transcript) – RNA that will be expressed by translation Spliceosome – cut introns, splice exons Large protein plus… snRNP (aka ‘Snurps’) Small nuclear ribonucleoproteins 150 nucleotides (snRNA) + p ...
... Introns (interrupt transcript) – long regions of noncoding RNA segments Exons (expressed transcript) – RNA that will be expressed by translation Spliceosome – cut introns, splice exons Large protein plus… snRNP (aka ‘Snurps’) Small nuclear ribonucleoproteins 150 nucleotides (snRNA) + p ...
The Science of Proteins in Milk (including A1 vs A2 Milk)
... relationship between BCM7 and etiology or cause of any suggested non-communicable diseases cannot be established.” Report to New Zealand Food Safety Authority (2004): “I do not believe there is sufficient evidence to warrant the government agencies taking further specific public health actions such ...
... relationship between BCM7 and etiology or cause of any suggested non-communicable diseases cannot be established.” Report to New Zealand Food Safety Authority (2004): “I do not believe there is sufficient evidence to warrant the government agencies taking further specific public health actions such ...
Plasma Membrane
... 1.Nucleus: membrane that surrounds DNA 2.Organelles: highly organized structures in the cells that serve a special function ...
... 1.Nucleus: membrane that surrounds DNA 2.Organelles: highly organized structures in the cells that serve a special function ...
MOLECULES OF LIFE
... Transport: move molecules around body and into and out of cells Support and structure: long fibrous proteins in hair nails skin Movement: contractile proteins in cells Regulation: proteins involved in maintaining homeostasis B. ...
... Transport: move molecules around body and into and out of cells Support and structure: long fibrous proteins in hair nails skin Movement: contractile proteins in cells Regulation: proteins involved in maintaining homeostasis B. ...
Amino Acids
... • Due to steric hindrance, all peptide bonds in proteins are in trans configuration • The 2 bonds around the α-carbon have freedom of rotation making proteins flexible to bend and fold ...
... • Due to steric hindrance, all peptide bonds in proteins are in trans configuration • The 2 bonds around the α-carbon have freedom of rotation making proteins flexible to bend and fold ...
biomolecule ppt
... • They consist of a carboxyl group (COOH) and an amino group NH2 • Peptide bonds form between amino acids (polypeptide = many peptide bonds = protein!) ...
... • They consist of a carboxyl group (COOH) and an amino group NH2 • Peptide bonds form between amino acids (polypeptide = many peptide bonds = protein!) ...
Protein Synthesis Continued
... After a strand of RNA is constructed by transcription, it must be altered before it moves to the cytoplasm Introns are sections of the RNA that do not code for a protein and are “cut out” of the RNA strand (they stay IN the nucleus) Exons are then spliced back together because they code for the prot ...
... After a strand of RNA is constructed by transcription, it must be altered before it moves to the cytoplasm Introns are sections of the RNA that do not code for a protein and are “cut out” of the RNA strand (they stay IN the nucleus) Exons are then spliced back together because they code for the prot ...
Transient expression assay in N. benthamiana leaves for
... Transient expression assay in N. benthamiana leaves for intracellular localization study Introduction Transient expression assay using N. benthamiana is an easy and simple way to examine intracellular localization of fluorescently-tagged proteins. Subcellular localization of proteins of one’s intere ...
... Transient expression assay in N. benthamiana leaves for intracellular localization study Introduction Transient expression assay using N. benthamiana is an easy and simple way to examine intracellular localization of fluorescently-tagged proteins. Subcellular localization of proteins of one’s intere ...
How do we get proteins? - Sebastian Charter Junior High
... DNA is used to make a single strand of RNA that is ...
... DNA is used to make a single strand of RNA that is ...
From Gene to Protein Genes code for... Proteins RNAs Remember
... Introns are removed from the mRNA transcript prior to it leaving the nucleus. This forms a mRNA transcript with a continuous coding sequence ...
... Introns are removed from the mRNA transcript prior to it leaving the nucleus. This forms a mRNA transcript with a continuous coding sequence ...
1. I can tell the difference between mRNA, tRNA, and rRNA
... DNA as a pattern. Once the gene is fully transcribed into RNA, the mRNA is edited. ...
... DNA as a pattern. Once the gene is fully transcribed into RNA, the mRNA is edited. ...
Chem 400 Biochemistry I
... •Biochemistry is essential to all of the life sciences (biomedical and plant sciences) All advanced degrees require that biochemistry is one of the first courses •This class will be taught not - as an advanced organic but as an encompassing science that should help tie several of your classes togeth ...
... •Biochemistry is essential to all of the life sciences (biomedical and plant sciences) All advanced degrees require that biochemistry is one of the first courses •This class will be taught not - as an advanced organic but as an encompassing science that should help tie several of your classes togeth ...
University of Groningen DNAJ proteins: more than just “co
... the flies when the incubators were accidently shifted to elevated temperature. Following up this serendipity, he showed that cells can activate a specific transcriptional program when exposed to elevated temperatures, later referred to as “heat shock response”. A decade later, Alfred Tissieres disco ...
... the flies when the incubators were accidently shifted to elevated temperature. Following up this serendipity, he showed that cells can activate a specific transcriptional program when exposed to elevated temperatures, later referred to as “heat shock response”. A decade later, Alfred Tissieres disco ...
TERTIARY STRUCTURE OF PROTEINS
... plasmin, TPA • All involve a serine in catalysis - thus the name • Ser is part of a "catalytic triad" of Ser, His, Asp • Serine proteases are homologous, but locations of the three crucial residues differ somewhat • Enzymologists agree, however, to number them always as His-57, Asp-102, Ser-195 ...
... plasmin, TPA • All involve a serine in catalysis - thus the name • Ser is part of a "catalytic triad" of Ser, His, Asp • Serine proteases are homologous, but locations of the three crucial residues differ somewhat • Enzymologists agree, however, to number them always as His-57, Asp-102, Ser-195 ...
Unit 5 practice FRQ #3 for final - KEY 3. 2009 AP Bio FRQ # 4 The
... Spliceosomes (snRNPs) Removes the introns and connects (splices) the exons in RNA Codons Codes for amino acids/signals RNA → _protein or site of protein synthesis Ribosomes tRNA Transports amino acids (b) Cells regulate both protein synthesis and protein activity. Discuss TWO specific mechanisms of ...
... Spliceosomes (snRNPs) Removes the introns and connects (splices) the exons in RNA Codons Codes for amino acids/signals RNA → _protein or site of protein synthesis Ribosomes tRNA Transports amino acids (b) Cells regulate both protein synthesis and protein activity. Discuss TWO specific mechanisms of ...
Molecular Genetics
... • The DNA of a person contains about 50,000 genes each of which codes for the making of a protein. • Hair and hemoglobin are proteins that your body makes. Enzymes are also proteins. • Everything in your body is based on the making of proteins! ...
... • The DNA of a person contains about 50,000 genes each of which codes for the making of a protein. • Hair and hemoglobin are proteins that your body makes. Enzymes are also proteins. • Everything in your body is based on the making of proteins! ...
Lecture 9b (2/18/13) "How to Make Proteins"
... 30S and 50S sub-units The smaller subunit binds to the mRNA, while the larger subunit binds to the tRNA and the amino acids. When a ribosome finishes reading a mRNA, these two subunits split apart. ...
... 30S and 50S sub-units The smaller subunit binds to the mRNA, while the larger subunit binds to the tRNA and the amino acids. When a ribosome finishes reading a mRNA, these two subunits split apart. ...
Model Description Sheet
... pathway, small RNAs derived from viruses are used by Ago-2 to slice virus mRNA, protecting the cells from infection. In the miRNA pathway, Ago-2 utilizes naturally occurring miRNA to slice cellular mRNAs to control protein production. Ago-2 works by binding small (~22 nucleotide) regulatory RNAs (si ...
... pathway, small RNAs derived from viruses are used by Ago-2 to slice virus mRNA, protecting the cells from infection. In the miRNA pathway, Ago-2 utilizes naturally occurring miRNA to slice cellular mRNAs to control protein production. Ago-2 works by binding small (~22 nucleotide) regulatory RNAs (si ...
Molecules of Life
... The food you eat, the silk a spider uses to make a web, the muscles in your body – all of these structures are made of macromolecules. Macromolecule is the term that biologists use for large molecules. There are four types of macromolecules that are important in biology: carbohydrates, lipids, prote ...
... The food you eat, the silk a spider uses to make a web, the muscles in your body – all of these structures are made of macromolecules. Macromolecule is the term that biologists use for large molecules. There are four types of macromolecules that are important in biology: carbohydrates, lipids, prote ...
SR protein
SR proteins are a conserved family of proteins involved in RNA splicing. SR proteins are named because they contain a protein domain with long repeats of serine and arginine amino acid residues, whose standard abbreviations are ""S"" and ""R"" respectively. SR proteins are 50-300 amino acids in length and composed of two domains, the RNA recognition motif (RRM) region and the RS binding domain. SR proteins are more commonly found in the nucleus than the cytoplasm, but several SR proteins are known to shuttle between the nucleus and the cytoplasm.SR proteins were discovered in the 1990s in Drosophila and in amphibian oocytes, and later in humans. In general, metazoans appear to have SR proteins and unicellular organisms lack SR proteins.SR proteins are important in constitutive and alternative pre-mRNA splicing, mRNA export, genome stabilization, nonsense-mediated decay, and translation. SR proteins alternatively splice pre-mRNA by preferentially selecting different splice sites on the pre-mRNA strands to create multiple mRNA transcripts from one pre-mRNA transcript. Once splicing is complete the SR protein may or may not remain attached to help shuttle the mRNA strand out of the nucleus. As RNA Polymerase II is transcribing DNA into RNA, SR proteins attach to newly made pre-mRNA to prevent the pre-mRNA from binding to the coding DNA strand to increase genome stabilization. Topoisomerase I and SR proteins also interact to increase genome stabilization. SR proteins can control the concentrations of specific mRNA that is successfully translated into protein by selecting for nonsense-mediated decay codons during alternative splicing. SR proteins can alternatively splice NMD codons into its own mRNA transcript to auto-regulate the concentration of SR proteins. Through the mTOR pathway and interactions with polyribosomes, SR proteins can increase translation of mRNA.Ataxia telangiectasia, neurofibromatosis type 1, several cancers, HIV-1, and spinal muscular atrophy have all been linked to alternative splicing by SR proteins.