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Lecture 3 - Transcription (student)
Lecture 3 - Transcription (student)

Gene Control
Gene Control

... be absorbed from environment 2. only producing proteins when needed a. don’t need lactose digesting enzymes ...
Chapter 19.
Chapter 19.

...  evolved to maintain constant internal conditions while facing changing external conditions ...
Bio1100Ch19W
Bio1100Ch19W

... These prevent cancer by repairing DNA or preventing rapid cell division If mutate a tumor suppressor gene- leads to ________ The Ras gene- (a protooncogene) is mutated in _____ of human ...
RNA polymerase I
RNA polymerase I

... in bacteria. • An operon consists of three elements: – the genes that it controls, • In bacteria, the genes coding for the enzymes of a particular pathway are clustered together and transcribed (or not) as one long mRNA molecule. – a promoter region where RNA polymerase first binds, – an operator re ...
DKN_5-8 TYPE
DKN_5-8 TYPE

... à  Different DNA polymerases have different fidelity! à  Error rate (wrong nucleotide inserted): 10-7 to 10-8 ...
Transcription Translation.notebook
Transcription Translation.notebook

PERSISTENCE: Mechanisms underlying the “Central Dogma
PERSISTENCE: Mechanisms underlying the “Central Dogma

... - Multiple polymerases with dedicated functions - Extensive post-transcriptional modifications - More regulatory sequences Will mitochondria and plastids transcribe DNA like Bacteria or like Eukarya? Why? ...
Presentation 1 Guidelines
Presentation 1 Guidelines

... may prevent transcription factors and/or RNA polymerase from binding to the major groove of the DNA. Second, it may prevent RNA polymerase from forming an open complex, which is necessary to begin transcription. Third, it could prevent looping in the DNA, which may be necessary to activate transcrip ...
Gene Editing - Royal Society of New Zealand
Gene Editing - Royal Society of New Zealand

... editing, or natural mutation. Gene editing technology is getting cheaper and easier, so is being used more frequently. While it can make more precise changes to genetic material than earlier techniques, there can still be unintended effects. As a result, while the technology offers exciting new oppo ...
Enzymes that cut DNA at specific base sequences. One type of
Enzymes that cut DNA at specific base sequences. One type of

... Having restriction enzymes that can cut in many different places allows genetic engineers to isolate genes for recombination and to cut DNA to create a DNA fingerprints ...
From Gene to Protein
From Gene to Protein

... • Eukaryotic cells have 3 kinds of RNA polymerase (I, II – used in RNA synthesis and III) • Bacteria have one kind – it makes not only mRNA but also other types of RNA • Bacteria have one chromosome and many plasmids. Information is constantly being sent to ribosomes for translation into proteins ne ...
Gene Section CRTC1 (CREB regulated transcription coactivator 1) Atlas of Genetics and Cytogenetics
Gene Section CRTC1 (CREB regulated transcription coactivator 1) Atlas of Genetics and Cytogenetics

... MECT1-MAML2; in the fusion protein the first 171 aa including the basic domain of MAML2 are replaced by 42 aa of MECT1; there are no sequence similarities in the N-terminal domains of MAML2 and MECT1; the fusion protein activates transcription of the Notch target gene HES1 independently of both Notc ...
Biology Packet 7:  DNA & RNA
Biology Packet 7: DNA & RNA

... Explain the function of DNA. Summarize the relationship between genes and DNA. Describe the overall structure of the DNA molecule. Describe the three components of a nucleotide. Explain the base pairing rules. Relate the role of the base pairing rules to the structure of DNA. Summarize the events of ...
Lecture_5
Lecture_5

... • Transcriptome - Expressed transcripts in a cell under defined experimental conditions. – mRNA(5-10% of total RNA). – rRNA, tRNA - make up most of total RNA – scRNA (protein secretion), tmRNA (rescue stalled ribosomes). ...
Prokaryotic Gene Expression Mechanisms RNA Types of RNA Other
Prokaryotic Gene Expression Mechanisms RNA Types of RNA Other

... repressor for lacO to 2 x 1010, but the affinity for random DNA sequences remains the same. So the specificity of repressor for lacO drops 3 orders of magnitude (or 1000-fold). Under these conditions, you can calculate that less than 3% of the lacO sites should have repressor bound to them (when IPT ...
Molecular Biology of Gene Function
Molecular Biology of Gene Function

... Laboratory Exercise: Mutational Analysis of the lac operon ...
Novagen • pET System Manual • 11th Edition
Novagen • pET System Manual • 11th Edition

... enhance the soluble yield of some target proteins. Another important benefit of this system is its ability to maintain target genes transcriptionally silent in the uninduced state. Target genes are initially cloned using hosts that do not contain the T7 RNA polymerase gene, thus eliminating plasmid ...
Science 103: Outline 17
Science 103: Outline 17

... Gene (DNA)  (b) Functions (i) (ii) (c) Location (d) Process A single-stranded RNA copy of the DNA is made by RNA polymerase: (i) RNA pol binds to and moves down the DNA, separating the strands. (ii) As it goes, it pairs complementary nucleotides with ONE strand, the template strand (*NOT coding str ...
Proteome and Gene Expression Analysis
Proteome and Gene Expression Analysis

... The Goals • Functional Genomics: – To know when, where and how much genes are expressed. – To know when, where, what kind and how much of each protein is present. ...
1495/Chapter 08
1495/Chapter 08

... both costs and benefits for cells. (8.1, 8.4) • The genetic code is redundant but not ambiguous. (8.1) • Many transcripts can be simultaneously produced from a single gene. (8.2) • Several types of processing are required before a pre-mRNA transcript can meet the needs of a eukaryotic cell. (8.2, 8. ...
TandT Group work
TandT Group work

... DNA Replication The bacterial cell needs to replicate its chromosome to make sure the new daughter cell has a complete copy for the genome. Using the key words below, draw a diagram and supplement with explanation where needed, that shows how DNA Replication occurs: ...
Chapter 8
Chapter 8

... Change of base pairs Change of size Change of function RNA ...
Chap 11 copy
Chap 11 copy

... good? • E. coli is able to turn off and on genes when lactose is present/absent • Bacteria saves resources and produce only those proteins that are needed ...
Biology: Protein Synthesis, Extra Credit Name: Place these
Biology: Protein Synthesis, Extra Credit Name: Place these

... Biology: Protein Synthesis, Extra Credit ...
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