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ranjan rajeev
ranjan rajeev

... of a bHLH transcription factor during anther development process in rice. Previously, by using microarray, qRT-PCR and promoter-reporter assay in transgenic system we reported OsbHLH142 as an anther specific gene in rice. Expression analysis of OsbHLH142 transcripts through qPCR and its protein prof ...
Phylogenomics of Cold Adaptation in Bacteria and Archaea
Phylogenomics of Cold Adaptation in Bacteria and Archaea

... temperatures are found widely throughout the tree of life, and the adaptations used to survive low temperature vary among these different groups. A paucity of genomic data on psychrophiles, cryophiles and their mesophilic relatives has to date made it difficult or impossible to assess the generality ...
introduction
introduction

... Validate motifs against 3D model No Secondary structure prediction No: use single sequence methods No: single sequence methods Motif search Secondary structure prediction Use other data ...
ppt
ppt

... Can transient transcription errors lead to phenotypic change that have long lasting consequences > Transient mutators: wild-type bacteria that exhibit a mutator phenotype due to transcription/translation errors Ninio suggests that a 1% subpopulation of cells is transiently deficient for a protein i ...
Lecture 14
Lecture 14

... ii. Bacteria capture circles of DNA with capacity to be copied iii. Evidence: mitochondria and chloroplasts have their own DNA circles iv. Multicellularity results in even more DNA per individual f. Where is DNA? i. Nucleus ii. DNA is long but extremely thin g. How is DNA packed? i. Similar to degre ...
Chap.1
Chap.1

... in the DNA sequence of a gene. Mutations in a gene's DNA sequence can alter the amino acid sequence of the protein encoded by the gene. How does this happen? Like words in a sentence, the DNA sequence of each gene determines the amino acid sequence for the protein it encodes. The DNA sequence is int ...
Techniques of gene therapy
Techniques of gene therapy

... be isolated, unless it is already available because it has been studied for some other purpose. Using an abnormal gene to find its normal counterpart is usually done by exploiting the extensive similarity between the sequences of the normal and defective genes; they rarely differ greatly in overall ...
Document
Document

... specific function (i.e. tubulin microtubules, catalase in cells, helicase to unwind DNA, etc.). There are only 20 amino acids; we are able to make 12 in our bodies (termed nonessential) and we must intake the other 8 in the food we eat (essential - isoleucine, leucine, lysine, methionine, phenylalan ...
Epigenetics Question
Epigenetics Question

... Is the gene tightly or loosely wrapped around histones? Are there many or few acetyl molecules attached to the gene? Are there many or few acetyl molecules attached to the histones? Are there many or few mRNA transcripts? ...
ap® biology 2012 scoring guidelines - AP Central
ap® biology 2012 scoring guidelines - AP Central

... This response earned the maximum of 3 points in part (a). One point was earned for describing the role of RNA splicing in protein synthesis: “[S]plicosome [sic] cuts segments out of the RNA strand … called introns.” One point was earned for describing the function of repressor proteins: “Repressor p ...
1) Semiconservative DNA replication means that A) each daughter
1) Semiconservative DNA replication means that A) each daughter

... B) nucleotides are constantly being recycled as cells make DNA. C) the cell can proofread its newly synthesized DNA only part of the time. D) each strand of a double-stranded DNA molecule is replicated differently 2) DNA helicases A) break hydrogen bonds between complementary nucleotides. B) synthes ...
Document
Document

... Plasmids carrying cloned eukaryotic genes can be introduced into animal cells grown in culture by transfection. In transient transfection (Fig. 5.32a), the introduced plasmid contains a viral replication origin, which allows it to propagate for a short time until diluted out in the cells due to inef ...
Slide 1
Slide 1

... the same gene, each from a different lab • submitters maintain editorial control over their records: what goes in is what comes out • no controlled vocabulary • variation in annotation of biological features Example: GenBank record ...
RNA nucleotides
RNA nucleotides

Topic 14: Protein Synthesis
Topic 14: Protein Synthesis

... specified by the gene sequence. How is this used to make protein? fig. 17.13- transfer RNA (tRNA); specialized RNA molecules that literally are involved in transferring the appropriate amino acid to the growing polypeptide chain 1. roughly 80 nucleotides long 2. at the 3’ end in a site where a parti ...
2 - UPCH
2 - UPCH

Slide 1
Slide 1

... the pest population 15% more than the current pesticide for a 10week period. A population of 50 beetles of the same species is exposed to the new pesticide for a 10-week period. A separate population of the same species of beetle is exposed to the same amount of the current pesticide for a 10-week p ...
DNA - California State University, Stanislaus
DNA - California State University, Stanislaus

R N A & PROTEIN SYNTHESIS
R N A & PROTEIN SYNTHESIS

... RNA is to deliver amino acids one by one to protein chains growing at ribosomes. ...
from innovative technologies ...to superior key products
from innovative technologies ...to superior key products

... Nucleic acids store and transfer genetic information in cells. The main types of nucleic acids are DNA and R NA, which are made up of chains of chemicals called nucleotides. Most DNA exists in cells as a double-stranded structure that resembles a twisted ladder. The nucleotides on opposite sides of ...
Chapter 15 Review Questions
Chapter 15 Review Questions

... a protein is its amino acid chain, bonded together with peptide bonds (amide linkages). The secondary structure of a protein begins to shape the amino acid chain using hydrogen bonding, forming alpha-helix and beta-pleated sheet structures. The tertiary structure of a protein gives it 3 dimensions. ...
Supporting Information S1: 1. Establishment of hSMP30 transcription
Supporting Information S1: 1. Establishment of hSMP30 transcription

... cooled to room temp and was reverse transcribed at 42ºC for 1hour using primer extension system (Promega, USA) according to manufacturer’s instruction. The same primer was used for the sequencing reactions of cloned SMP30 promoter containing exon 1. Sequencing reactions and primer extension product ...
Bioinformatics Session - March 1, 2014 - 9:00am – 12:00pm
Bioinformatics Session - March 1, 2014 - 9:00am – 12:00pm

... the most well-known cystic fibrosis related gene? 5. Click the ‘Gene/Locus MIM Number” for the CFTR gene in the table. ...
Genomic and cDNA libraries, library screening
Genomic and cDNA libraries, library screening

... •Using a DNA probe with a homologous sequence (e.g., a homologous cDNA or gene clone from a related species) •Using an oligonucleotide probe based on a known amino acid sequence (requires purification of the protein and some peptide sequencing) •Using an antibody against the protein of interest (not ...
20-DNA-technology
20-DNA-technology

... read under UV-light ...
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