Kein Folientitel - Chair of Soil Science
... • First RT-PCR on soil RNA followed by cDNA amplification was The primers detect laccases in a broad range of Basidiomycota realized. Additional sample analyses and sequencing should of all functional groups (saprophytes, pathogens & mycorrhiza) confirm of the effectiveness of the method Contact: ...
... • First RT-PCR on soil RNA followed by cDNA amplification was The primers detect laccases in a broad range of Basidiomycota realized. Additional sample analyses and sequencing should of all functional groups (saprophytes, pathogens & mycorrhiza) confirm of the effectiveness of the method Contact: ...
Gene Section EIF3A (eukaryotic translation initiation factor 3, subunit A)
... (Pincheira et al., 2001b). It has phosphorylation sites at Ser-881, Ser-1198, Ser-1336 and Ser-1364 (Damoc et al., 2007). The PCI domain spans from amino acid 405 to 495, which contains purely alpha-helix (Pincheira et al., 2001b). Since most of the proteins containing this domain are part of a mult ...
... (Pincheira et al., 2001b). It has phosphorylation sites at Ser-881, Ser-1198, Ser-1336 and Ser-1364 (Damoc et al., 2007). The PCI domain spans from amino acid 405 to 495, which contains purely alpha-helix (Pincheira et al., 2001b). Since most of the proteins containing this domain are part of a mult ...
Investigation 1: Examining RNA-Seq data
... We will continue to focus on isoform A of transformer (referred to as tra-RA). Here we will focus on data from experiments that assess the RNA population in cells. This data can be used to help us identify exons and introns for the gene under study. All RNAs in the cell are collectively known as the ...
... We will continue to focus on isoform A of transformer (referred to as tra-RA). Here we will focus on data from experiments that assess the RNA population in cells. This data can be used to help us identify exons and introns for the gene under study. All RNAs in the cell are collectively known as the ...
Animal mitochondrial biogenesis and function
... The mitochondrial genome is replicated and transcribed within the organelle. Both mtDNA replication and transcription have been characterised mainly in mammals where the cis-elements responsible for the regulation of these processes are mostly located within a small non-coding DNA fragment, the D-lo ...
... The mitochondrial genome is replicated and transcribed within the organelle. Both mtDNA replication and transcription have been characterised mainly in mammals where the cis-elements responsible for the regulation of these processes are mostly located within a small non-coding DNA fragment, the D-lo ...
TFIIH Plays an Essential Role in RNA Polymerase I Transcription
... TIF-IB. When assayed in the presence of TIF-IBIIH, however, transcriptional activity of TFIIH-deficient Pol I compared to that of Pol IIIH, indicating that specific Pol I transcription requires the presence of TFIIH. As a second approach to compare the activity of TFIIH-deficient and TFIIH-containin ...
... TIF-IB. When assayed in the presence of TIF-IBIIH, however, transcriptional activity of TFIIH-deficient Pol I compared to that of Pol IIIH, indicating that specific Pol I transcription requires the presence of TFIIH. As a second approach to compare the activity of TFIIH-deficient and TFIIH-containin ...
The transcription factor AtDOF4.2 regulates shoot branching and
... DOF (DNA-binding with one finger) proteins are a group of plantspecific transcription factors. A typical DOF protein consists of a conserved N-terminal DNA BD (binding domain), a divergent C-terminal end for transcriptional regulation and the conjunctive sequences with a possible NLS (nuclear locali ...
... DOF (DNA-binding with one finger) proteins are a group of plantspecific transcription factors. A typical DOF protein consists of a conserved N-terminal DNA BD (binding domain), a divergent C-terminal end for transcriptional regulation and the conjunctive sequences with a possible NLS (nuclear locali ...
PDF only
... Genia corpus (Ohtae et al. 2001) is not specialized on a specific species whereas ours is based on Bacillus Subtilis. Both projects develop annotation tools and Document Type Definition (DTD), which are, for the most part, compatible. The aim here is to build training corpus to which various techniq ...
... Genia corpus (Ohtae et al. 2001) is not specialized on a specific species whereas ours is based on Bacillus Subtilis. Both projects develop annotation tools and Document Type Definition (DTD), which are, for the most part, compatible. The aim here is to build training corpus to which various techniq ...
Proteogenomics - The Fenyo Lab
... heavily on the quality of the protein sequence database (DB) • DBs with missing peptide sequences will fail to identify the corresponding peptides • DBs that are too large will have low sensitivity • Ideal DB is complete and small, containing all proteins in the sample and no irrelevant sequences ...
... heavily on the quality of the protein sequence database (DB) • DBs with missing peptide sequences will fail to identify the corresponding peptides • DBs that are too large will have low sensitivity • Ideal DB is complete and small, containing all proteins in the sample and no irrelevant sequences ...
Biology 11.1 Gene Technology
... In 1973, Stanley Cohen and Herbert Boyer conducted an experiment that revolutionized genetic studies in biology. They isolated the gene that codes for ribosomal RNA from the DNA of an African clawed frog and than inserted it into the DNA of E. Coli bacteria. During transcription, the bacteria produc ...
... In 1973, Stanley Cohen and Herbert Boyer conducted an experiment that revolutionized genetic studies in biology. They isolated the gene that codes for ribosomal RNA from the DNA of an African clawed frog and than inserted it into the DNA of E. Coli bacteria. During transcription, the bacteria produc ...
Gene Section
... mitochondrial), centromeric to DMC1 (dosage suppressor of mck1, yeast homologue meiosis-specific homologous recombination). ...
... mitochondrial), centromeric to DMC1 (dosage suppressor of mck1, yeast homologue meiosis-specific homologous recombination). ...
medline:93194904
... The cellular oncogene c-myb can interact synergistically with the Epstein-Barr virus BZLF1 transactivator in lymphoid cells. Regulation of replicative functions in the Epstein-Barr virus (EBV) genome is mediated through activation of a virally encoded transcription factor, Z (BZLF1). We have shown t ...
... The cellular oncogene c-myb can interact synergistically with the Epstein-Barr virus BZLF1 transactivator in lymphoid cells. Regulation of replicative functions in the Epstein-Barr virus (EBV) genome is mediated through activation of a virally encoded transcription factor, Z (BZLF1). We have shown t ...
Visualization of RNA molecules using VMD
... thymine as a base pair to adenine. It is also single-stranded in most cases, while DNA is double-stranded. The sugar is ribose in RNA and deoxyribose in DNA. ...
... thymine as a base pair to adenine. It is also single-stranded in most cases, while DNA is double-stranded. The sugar is ribose in RNA and deoxyribose in DNA. ...
CLONING A LYSINE-RICH PROTEIN GENE FROM POTATO
... ABSTRACT: Lysine is one of the limiting essential amino acids because it is not synthesized in the body of animals and human. They must obtain lysine from their diet. Recent results of gene transfer research showed the possibility of overexpression of genes encoding natural lysine-rich proteins in c ...
... ABSTRACT: Lysine is one of the limiting essential amino acids because it is not synthesized in the body of animals and human. They must obtain lysine from their diet. Recent results of gene transfer research showed the possibility of overexpression of genes encoding natural lysine-rich proteins in c ...
Control in cells and in organisms June 2011
... Additional notes on marking Question 10 Care must be taken in using these notes. It is important to appreciate that the only criteria to be used in awarding marks to a particular essay are those corresponding to the appropriate descriptors. Candidates may gain credit for any information providing th ...
... Additional notes on marking Question 10 Care must be taken in using these notes. It is important to appreciate that the only criteria to be used in awarding marks to a particular essay are those corresponding to the appropriate descriptors. Candidates may gain credit for any information providing th ...
Role of Integration Host Factor in the Transcriptional Activation of
... significantly reduced amounts of the class IV flagellin, FljL, as a consequence of reduced transcriptional activity. Our results indicate that the motility defect exhibited by the ⌬ihfB strain is most likely attributable to its failure to accumulate the class IV-encoded 27-kDa flagellin subunit, Flj ...
... significantly reduced amounts of the class IV flagellin, FljL, as a consequence of reduced transcriptional activity. Our results indicate that the motility defect exhibited by the ⌬ihfB strain is most likely attributable to its failure to accumulate the class IV-encoded 27-kDa flagellin subunit, Flj ...
Reading the Blueprint of Life Chromosome DNA Gene Transcription
... Reading the Blueprint of Life: Translation 1. mRNA must be decoded by the ribosome Message from DNA the Gene! Instructions to ribosome on how to assemble a protein mRNA Code words are called Codons Codons are 3 base pairs long Every message has a start codon Every message has a stop cod ...
... Reading the Blueprint of Life: Translation 1. mRNA must be decoded by the ribosome Message from DNA the Gene! Instructions to ribosome on how to assemble a protein mRNA Code words are called Codons Codons are 3 base pairs long Every message has a start codon Every message has a stop cod ...
National Exam
... How might Phe1324 and Ile1270 contribute to the structure and function of the Cas9 protein? (2 pts) These sidechains are hydrophobic in nature – and are closely packed in the inside of a sub-‐domain ...
... How might Phe1324 and Ile1270 contribute to the structure and function of the Cas9 protein? (2 pts) These sidechains are hydrophobic in nature – and are closely packed in the inside of a sub-‐domain ...
Role of Deoxyribonucleic Acid Polymerase beta in Nuclear
... Use of type (i) DNA has been largely restricted to work with prokaryotic polymerases of the poll class, as these are the only enzymes so far described which are capable of the strand displacement and/or 5’: 3’ hydrolysis necessary for extensive synthesis on this template (Kornberg, 1974). Type (ii) ...
... Use of type (i) DNA has been largely restricted to work with prokaryotic polymerases of the poll class, as these are the only enzymes so far described which are capable of the strand displacement and/or 5’: 3’ hydrolysis necessary for extensive synthesis on this template (Kornberg, 1974). Type (ii) ...
L3 - DNA Translation (Protein Synthesis
... • Once the ribosome reaches a termination codon, termination begins • The now empty ribosome then dissociates and can then bind to another strand of mRNA to begin again. • Several ribosomes can be attached to an mRNA strand at any one time resulting in the formation of many peptide chains at the sam ...
... • Once the ribosome reaches a termination codon, termination begins • The now empty ribosome then dissociates and can then bind to another strand of mRNA to begin again. • Several ribosomes can be attached to an mRNA strand at any one time resulting in the formation of many peptide chains at the sam ...
The Arabidopsis chloroplast ribosomal protein L21 is
... To examine the origin of chloroplast L21 protein in different plant species, we compared the amino acid sequences of the conserved core regions. RPL21c is closer to RPL21m (46% identities) than to the chloroplast-encoded L21 protein of M. polymorpha (31%). Using parsimony, neighbor-joining and Fitch ...
... To examine the origin of chloroplast L21 protein in different plant species, we compared the amino acid sequences of the conserved core regions. RPL21c is closer to RPL21m (46% identities) than to the chloroplast-encoded L21 protein of M. polymorpha (31%). Using parsimony, neighbor-joining and Fitch ...
Phage, colicins and macroregulatory phenomena
... reform the functional structures in vitro (as in the assembly of viral shells or of bacterial flagella from monomeric proteins) or if the preexisting pattern of molecular organization plays some role in the orderly accretion of new functional elements in the membrane of a growing cell - a priming ro ...
... reform the functional structures in vitro (as in the assembly of viral shells or of bacterial flagella from monomeric proteins) or if the preexisting pattern of molecular organization plays some role in the orderly accretion of new functional elements in the membrane of a growing cell - a priming ro ...
Polymerase Chain Reaction (PCR)
... For example, if a technician in a crime lab set up a test reaction (with blood from the crime scene) after setting up a positive control reaction (with blood from the suspect) cross contamination between the samples could result in an erroneous incrimination, even if the technician changed pipette t ...
... For example, if a technician in a crime lab set up a test reaction (with blood from the crime scene) after setting up a positive control reaction (with blood from the suspect) cross contamination between the samples could result in an erroneous incrimination, even if the technician changed pipette t ...
Procedure - DNA Interactive
... 1940's most of her colleagues assumed the genome was a static entity- stable genes, replicating faithfully as cells divided and organisms developed. Observations made by McClintock at Cold Spring Harbor Laboratory told a radically different story. Although not immediately accepted by many of her fel ...
... 1940's most of her colleagues assumed the genome was a static entity- stable genes, replicating faithfully as cells divided and organisms developed. Observations made by McClintock at Cold Spring Harbor Laboratory told a radically different story. Although not immediately accepted by many of her fel ...
Titel: Functional replacement of Gfi1 deficiency by Gfi1b obviously
... expected, but was clearly present in Gfi1+/1b and at a significantly higher level also in Gfi11b/1b animals. While these results confirm that functional Gfi1b alleles have been generated at the Gfi1 locus, the relatively increased Gfi1b protein level in Gfi11b/1b thymocytes indicates a regulatory ph ...
... expected, but was clearly present in Gfi1+/1b and at a significantly higher level also in Gfi11b/1b animals. While these results confirm that functional Gfi1b alleles have been generated at the Gfi1 locus, the relatively increased Gfi1b protein level in Gfi11b/1b thymocytes indicates a regulatory ph ...
18 - cloudfront.net
... The idea behind PCR is surprisingly simple. At one end of a piece of DNA a biologist wants to copy, he or she adds a short piece of DNA that is complementary to a portion of the sequence. At the other end, the biologist adds another short piece of complementary DNA. These short pieces are known as " ...
... The idea behind PCR is surprisingly simple. At one end of a piece of DNA a biologist wants to copy, he or she adds a short piece of DNA that is complementary to a portion of the sequence. At the other end, the biologist adds another short piece of complementary DNA. These short pieces are known as " ...