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Moonlighting proteins—an update
Moonlighting proteins—an update

... perform these different functions.11 The helical La motif domain binds to the 3 0 UUU-OH of RNA to protect the RNA from 3 0 -end digestion. Another domain, RRM1, with helices and a beta-sheet, is also needed in a second function as a chaperone to assist in RNA folding. Yeast cytoplasmic peroxiredoxi ...
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... MEDICAL AND BIOLOGICAL IMPORTANCE 1. Nucleic acids serve as genetic material of living organisms including humans. 2. Nucleic acids are involved in the storage, transfer and expression of genetic information. 3. Nucleic acids contain all the necessary information required for the formation of indivi ...
Fan et al.P65
Fan et al.P65

... critical domains that are thought to mediate its proposed function as a transcriptional repressor. The three domains are: 1) the methyl CpG binding domain (MBD) that confers binding to methylated CpG dinucleotides [23]; 2) a transcriptional repression domain (TRD), which interacts with various co-re ...
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A Statistical Method for Finding Transcriptional Factor Binding Sites

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Anna Yu`s ppt - The University of Texas at Austin
Anna Yu`s ppt - The University of Texas at Austin

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... In some retroviruses, for example Moloney murlne leukemia virus (MoMLV) [9], there is one copy of NBPcys whilst In others, for example Rous sarcoma virus (RSV) [10], the sequence is duplicated in tandem. Because NBPcys is so highly conserved in both sequence and positional terms, I undertook a searc ...
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MB206_fhs_lnt_001.1_AT_May09

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Brooker Chapter 18 - Volunteer State Community College

... Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display ...
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Concept 14.4: Translation is the RNA

... © 2014 Pearson Education, Inc. ...
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... Tn21 there is an integron encoding streptomycin and sulphonamide resistance. While the aforementioned are typically extrachromosomal genes, plasmids can also carry chromosomal genes. A good example is the class C chromosomal β-lactamases, whose genes are increasingly found on plasmids. As mentioned ...
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... Not all environmental factors are mutagens, eg wind is not a mutagen because it doesn’t change the DNA sequence but does change phenotype. Genotype is not the only factor influencing gene expression however, as the phenotype of an organism results from the interaction between the genotype and the en ...
Spotting the enemy within: Targeted silencing of foreign DNA in
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... To date, the vast majority of KRAB-ZFP research has focused on human and mouse KRAB-ZFPs. Therefore, it is important to point out that the findings from these studies may not always be applicable to tetrapod KRABZFPs in general. Nevertheless, all KRAB-ZFPs contain tandem arrays of up to 36 C2H2-type ...
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... Evaluating the cuts of a Restriction Enzyme • Palindromes in DNA occur randomly throughout the genome which allows for multiple cuts with a single restriction enzyme. Calculating # of cuts • 4 bp enzyme occurs ~300bp • 6 bb enzyme cut ~3000bp • Lambda genome is ~48500bp while its plasmid is ~6000bp ...
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Chapter 21 Notes

... ○ They looked for protein-coding genes and genes for noncoding RNAs as well as sequences that regulate DNA replication, gene expression (such as enhancers and promoters), and chromatin modifications. ...
Intrinsically unstructured proteins
Intrinsically unstructured proteins

... internal molecular dynamics in the unstructured state. The other technique that is used most often is far-UV circular dichroism (CD) spectroscopy, which detects the amount (or lack) of secondary structure. The ellipticity spectrum of IUPs has a large negative peak at around 200 nm and a value close ...
The sigA gene encoding the major G factor of RNA polymerase from
The sigA gene encoding the major G factor of RNA polymerase from

... Keywords: Jynechococcz/s sp., RNA polymerase, sigma factors, cyanobacteria ...
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Transcriptional regulation

In molecular biology and genetics, transcriptional regulation is the means by which a cell regulates the conversion of DNA to RNA (transcription), thereby orchestrating gene activity. A single gene can be regulated in a range of ways, from altering the number of copies of RNA that are transcribed, to the temporal control of when the gene is transcribed. This control allows the cell or organism to respond to a variety of intra- and extracellular signals and thus mount a response. Some examples of this include producing the mRNA that encode enzymes to adapt to a change in a food source, producing the gene products involved in cell cycle specific activities, and producing the gene products responsible for cellular differentiation in higher eukaryotes.The regulation of transcription is a vital process in all living organisms. It is orchestrated by transcription factors and other proteins working in concert to finely tune the amount of RNA being produced through a variety of mechanisms. Prokaryotic organisms and eukaryotic organisms have very different strategies of accomplishing control over transcription, but some important features remain conserved between the two. Most importantly is the idea of combinatorial control, which is that any given gene is likely controlled by a specific combination of factors to control transcription. In a hypothetical example, the factors A and B might regulate a distinct set of genes from the combination of factors A and C. This combinatorial nature extends to complexes of far more than two proteins, and allows a very small subset (less than 10%) of the genome to control the transcriptional program of the entire cell.
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