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Chapter 20 DNA Technology
... *** CAN BE USED TO SEPARATE ANY MOLECULE WITH A CHARGE ~ not just for DNA (Ex: proteins) •DNA is negatively charged (due to phosphates) so moves in electric field •Used to separate DNA fragments after cut with restriction enzymes •Separates by size and electric charge •Can identify DNA molecules by ...
... *** CAN BE USED TO SEPARATE ANY MOLECULE WITH A CHARGE ~ not just for DNA (Ex: proteins) •DNA is negatively charged (due to phosphates) so moves in electric field •Used to separate DNA fragments after cut with restriction enzymes •Separates by size and electric charge •Can identify DNA molecules by ...
7.4 Biotechnology Outline
... This process is used to create a “DNA fingerprint”. A. Different individual’s DNA samples, but from the same region of a chromosome are exposed to the same restriction enzyme. 1. This creates Restriction Fragment Length Polymorphisms (RFLP’s) a. These are fragments of DNA having different lengths th ...
... This process is used to create a “DNA fingerprint”. A. Different individual’s DNA samples, but from the same region of a chromosome are exposed to the same restriction enzyme. 1. This creates Restriction Fragment Length Polymorphisms (RFLP’s) a. These are fragments of DNA having different lengths th ...
Ch. 16 Molecular Basis Heredity AND Replication Activity
... Purines: ‘A’ & ‘G’ Pyrimidines: ‘C’ & ‘T’ (Chargaff rules) ‘A’ H+ bonds (2) with ‘T’ ‘C’ H+ bonds (3) with ‘G’ Van der Waals attractions between the stacked pairs ...
... Purines: ‘A’ & ‘G’ Pyrimidines: ‘C’ & ‘T’ (Chargaff rules) ‘A’ H+ bonds (2) with ‘T’ ‘C’ H+ bonds (3) with ‘G’ Van der Waals attractions between the stacked pairs ...
Recombinant DNA Technology
... pathways to produce biological substitutes for less desirable chemical processes; allowing greater agricultural production production, permitting more efficient and safer energy production, and; providing better understanding of the metabolic basis for some medical conditions that could assist in th ...
... pathways to produce biological substitutes for less desirable chemical processes; allowing greater agricultural production production, permitting more efficient and safer energy production, and; providing better understanding of the metabolic basis for some medical conditions that could assist in th ...
DNAInternet webquest
... How many complete molecules of DNA do you begin with in DNA replication? _________________ How many DNA molecules do you end up with? ________________________ Is the new DNA molecules completely new? Explain. _____________________________________ ...
... How many complete molecules of DNA do you begin with in DNA replication? _________________ How many DNA molecules do you end up with? ________________________ Is the new DNA molecules completely new? Explain. _____________________________________ ...
What is DNA Computing?
... The natural proclivity of specific DNA molecules to chemically interact according to defined rules to produce new molecules Laboratory techniques that allow the isolation/identification of product molecules with specific properties PCR, Ligation, Gel Electrophoresis, etc. ...
... The natural proclivity of specific DNA molecules to chemically interact according to defined rules to produce new molecules Laboratory techniques that allow the isolation/identification of product molecules with specific properties PCR, Ligation, Gel Electrophoresis, etc. ...
DNA Structure
... -The DNA molecule splits into 2 at the replication points, unzipping due to the enzymes breaking the hydrogen bonds. The DNA polymerase then produces 2 new complementary strands following the rules of base paring by joining individual nucleotides to produce a DNA molecule. Each strand serves as a te ...
... -The DNA molecule splits into 2 at the replication points, unzipping due to the enzymes breaking the hydrogen bonds. The DNA polymerase then produces 2 new complementary strands following the rules of base paring by joining individual nucleotides to produce a DNA molecule. Each strand serves as a te ...
Human Genome
... Is there a way to prevent self-ligation of linearized plasmid ? If No explain why, if Yes explain how. ...
... Is there a way to prevent self-ligation of linearized plasmid ? If No explain why, if Yes explain how. ...
Cloning the Progesterone 5 beta- reductase gene
... * Repeat the wash two times. * Dry the pellet (with bound DNA). * Resuspend the pellet in 50 µL distilled water. Incubate at 50-65°C for 5 min. * Spin down pellet and transfer the eluted DNA to a new eppendorf tube. ...
... * Repeat the wash two times. * Dry the pellet (with bound DNA). * Resuspend the pellet in 50 µL distilled water. Incubate at 50-65°C for 5 min. * Spin down pellet and transfer the eluted DNA to a new eppendorf tube. ...
Name
... o Energy flow p789 o Analyze food chains and food webs p791 o Types of population growth p770 o Biogeochemical cycles p796-801 Essay: Evaluate a food web p790 >explain relationship between organisms >form a hypothesis >design an experiment >identify controls & variables ...
... o Energy flow p789 o Analyze food chains and food webs p791 o Types of population growth p770 o Biogeochemical cycles p796-801 Essay: Evaluate a food web p790 >explain relationship between organisms >form a hypothesis >design an experiment >identify controls & variables ...
LAB 2 LECTURE The Molecular Basis for Species Diversity DNA
... B. The central dogma1. DNA⇐ (replication) ⇐ DNA ⇒ (transcription) ⇒ RNA ⇒ (translation) ⇒ Proteins 2. In words, DNA is the material that contains the hereditary information. a. It is capable of reproducing itself – DNA replication b. It can supervise the manufacture of RNA – transcription. c. The re ...
... B. The central dogma1. DNA⇐ (replication) ⇐ DNA ⇒ (transcription) ⇒ RNA ⇒ (translation) ⇒ Proteins 2. In words, DNA is the material that contains the hereditary information. a. It is capable of reproducing itself – DNA replication b. It can supervise the manufacture of RNA – transcription. c. The re ...
Agarose gel electrophoresis
![](https://commons.wikimedia.org/wiki/Special:FilePath/DNAgel4wiki.png?width=300)
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.Agarose gels are easy to cast and are particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7 - 2% dissolved in a suitable electrophoresis buffer.