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Recombinant DNA Technology
... BAC is a DNA construct, based on a fertility plasmid (or F-plasmid), used for transforming and cloning in bacteria, usually E. coli. F-plasmids play a crucial role because they contain partition genes that promote the even distribution of plasmids after bacterial cell division. BAC's usual insert si ...
... BAC is a DNA construct, based on a fertility plasmid (or F-plasmid), used for transforming and cloning in bacteria, usually E. coli. F-plasmids play a crucial role because they contain partition genes that promote the even distribution of plasmids after bacterial cell division. BAC's usual insert si ...
Protocol: Cloning of oligos for sgRNA (CRISPR) or
... The 7kb open-vector band is excised and the vector is extracted using Qiaquick Gel Extraction kit (Qiagen). The extracted DNA is suspended into a final volume of 2000ul of TE buffer (about 6 ng per ul). The opened vector is then stored frozen. Note: When stuffed-pLKO is used to prep the linear vecto ...
... The 7kb open-vector band is excised and the vector is extracted using Qiaquick Gel Extraction kit (Qiagen). The extracted DNA is suspended into a final volume of 2000ul of TE buffer (about 6 ng per ul). The opened vector is then stored frozen. Note: When stuffed-pLKO is used to prep the linear vecto ...
Replication of DNA.
... Transcription: only one of the DNA strands is copied (coding or antisense strand). An RNA polymerase replicates the DNA sequence into a complementary sequence of mRNA (template or sense strand). mRNAs are transported from the nucleus to the cytoplasm, where they acts as the template for protein bio ...
... Transcription: only one of the DNA strands is copied (coding or antisense strand). An RNA polymerase replicates the DNA sequence into a complementary sequence of mRNA (template or sense strand). mRNAs are transported from the nucleus to the cytoplasm, where they acts as the template for protein bio ...
12.2 DNA and Technology
... What is Since the discovery of DNA, scientists have found new methods of genetic producing organisms with desired traits. One of those methods is engineering? called genetic engineering. Genetic engineering is the process of transferring genes from one organism into the DNA of another organism. Walk ...
... What is Since the discovery of DNA, scientists have found new methods of genetic producing organisms with desired traits. One of those methods is engineering? called genetic engineering. Genetic engineering is the process of transferring genes from one organism into the DNA of another organism. Walk ...
fall break, take home exam
... books (consider bookshelf at the NCBI website), information and software available though class or from the internet (google, youtube) to answer the questions below. Unless indicated otherwise, answer within the space provided. Provide only the answer that you think is correct. In case of a correct ...
... books (consider bookshelf at the NCBI website), information and software available though class or from the internet (google, youtube) to answer the questions below. Unless indicated otherwise, answer within the space provided. Provide only the answer that you think is correct. In case of a correct ...
Cloning of recombinant DNA: using vectors
... Taxol. Treatment for ovarian cancer Interferon. Treatment for cancer and viral infections In addition, a number of vaccines are now commercially prepared from recombinant hosts. At one time vaccines were made by denaturing the disease and then injecting it into humans with the hope that it would ...
... Taxol. Treatment for ovarian cancer Interferon. Treatment for cancer and viral infections In addition, a number of vaccines are now commercially prepared from recombinant hosts. At one time vaccines were made by denaturing the disease and then injecting it into humans with the hope that it would ...
Protocols - BioMed Central
... 2. Check the success of the PCR reaction by loading 5 µl of the reaction volume on an agarose gel. Note that it is not necessary to purify the PCR amplicon before the USER Friendly cloning reaction. 3. Mix the following in a 0.2 ml PCR tube PCR product 1 10 l PCR product 2 10 l Linearized vector 4 ...
... 2. Check the success of the PCR reaction by loading 5 µl of the reaction volume on an agarose gel. Note that it is not necessary to purify the PCR amplicon before the USER Friendly cloning reaction. 3. Mix the following in a 0.2 ml PCR tube PCR product 1 10 l PCR product 2 10 l Linearized vector 4 ...
Chapter 3 – Research results
... luminescence features of semiconductor quantum dots (QDs) nanoparticles were extensively used to develop biosensor platforms. Specifically, the aggregation of metallic nanoparticles and the color changes accompanying the transitions upon aggregation and deaggregation of the NPs were broadly impleme ...
... luminescence features of semiconductor quantum dots (QDs) nanoparticles were extensively used to develop biosensor platforms. Specifically, the aggregation of metallic nanoparticles and the color changes accompanying the transitions upon aggregation and deaggregation of the NPs were broadly impleme ...
2420 Topics for Examination II
... FOR BIOTECHNOLOGY. What are restriction endonucleases? What is special about the way they cleave DNA? From which kind of microorganisms are all restriction endonucleases obtained? How will DNA fragments of different sizes migrate through agarose gels, and how will they sort themselves by size? How i ...
... FOR BIOTECHNOLOGY. What are restriction endonucleases? What is special about the way they cleave DNA? From which kind of microorganisms are all restriction endonucleases obtained? How will DNA fragments of different sizes migrate through agarose gels, and how will they sort themselves by size? How i ...
Supplementary Information (doc 38K)
... of the plasmid pCMV-BRCA1 that expresses BRCA1, and 0.05 g of internal control plasmid pRL-SV40 was transfected into p53-/- or p53-/-Atf-2-/- cells using Lipofectamine (Invitrogen), and luciferase assays were performed. The total amount of plasmid DNA was adjusted to 4.05 g by adding control plasm ...
... of the plasmid pCMV-BRCA1 that expresses BRCA1, and 0.05 g of internal control plasmid pRL-SV40 was transfected into p53-/- or p53-/-Atf-2-/- cells using Lipofectamine (Invitrogen), and luciferase assays were performed. The total amount of plasmid DNA was adjusted to 4.05 g by adding control plasm ...
recombinant dna
... ‘fingerprinting’ and diagnosis of infectious diseases. For example, we can detect the presence of genetic sequences unique to hepatitis B virus in a blood sample of an infected patient even before the patient shows symptoms or an immune response. Southern blotting also were use to demonstrate the ...
... ‘fingerprinting’ and diagnosis of infectious diseases. For example, we can detect the presence of genetic sequences unique to hepatitis B virus in a blood sample of an infected patient even before the patient shows symptoms or an immune response. Southern blotting also were use to demonstrate the ...
Agarose gel electrophoresis
![](https://commons.wikimedia.org/wiki/Special:FilePath/DNAgel4wiki.png?width=300)
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.Agarose gels are easy to cast and are particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7 - 2% dissolved in a suitable electrophoresis buffer.