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Chap 4 Chemical Synhesis Sequencing and Amplification of DNA
... LIC is a cloning method that makes use of annealing of single-stranded complementary overhangs on the target vector and a PCR-generated insert of at least 12 bases. The commercial InfusionTM system (Clontech) is based on the same principle and requires a 15-base overlap region. S.s. overhangs can be ...
... LIC is a cloning method that makes use of annealing of single-stranded complementary overhangs on the target vector and a PCR-generated insert of at least 12 bases. The commercial InfusionTM system (Clontech) is based on the same principle and requires a 15-base overlap region. S.s. overhangs can be ...
Unit 5 quesitons
... 38. Name and describe the different point mutations. 39. What are restriction enzymes and what natural purpose do they serve? 40. What is a vector? Name two common vectors that are used in DNA technology. 41. Briefly outline the steps involved in using bacteria and plasmids to clone a gene. 42. How ...
... 38. Name and describe the different point mutations. 39. What are restriction enzymes and what natural purpose do they serve? 40. What is a vector? Name two common vectors that are used in DNA technology. 41. Briefly outline the steps involved in using bacteria and plasmids to clone a gene. 42. How ...
Simple and chemical DNA extraction from preserved bivalve mantle
... Genomic DNA preparation for PCR amplification has become one of the major concerns in molecular-based phylogenetic analysis for wild populations and cultured broodstocks of commercially important bivalves, especially when a large number of samples must be processed. From ark shell and scallop (3) an ...
... Genomic DNA preparation for PCR amplification has become one of the major concerns in molecular-based phylogenetic analysis for wild populations and cultured broodstocks of commercially important bivalves, especially when a large number of samples must be processed. From ark shell and scallop (3) an ...
Chapter 13
... The Southern blot is a technique that uses radioactively labeled RNA or single-stranded DNA as a "probe" to identify a specific gene. In a Southern blot, the cloned DNA is first cleaved into fragments by restriction enzymes The fragments are separated by gel electrophoresis a technique that uses an ...
... The Southern blot is a technique that uses radioactively labeled RNA or single-stranded DNA as a "probe" to identify a specific gene. In a Southern blot, the cloned DNA is first cleaved into fragments by restriction enzymes The fragments are separated by gel electrophoresis a technique that uses an ...
the link to our brochure
... forensic expertise with new and innovative approaches to solving crime. By working with some of the UK’s busiest rape and sexual assault police investigation teams, we have developed an approach that combines a rapid, responsive service, with technology innovation that has pushed the boundaries of d ...
... forensic expertise with new and innovative approaches to solving crime. By working with some of the UK’s busiest rape and sexual assault police investigation teams, we have developed an approach that combines a rapid, responsive service, with technology innovation that has pushed the boundaries of d ...
Chapter 10 Manipulating Genes
... strand DNA only at particular sites, determined by short sequence of nucleotide pairs. Restriction nucleases can therefore be used to produce a reproducible set specific DNA fragments from any genome. The main reason they are so useful is that a given enzyme will always cut a given DNA molecule at t ...
... strand DNA only at particular sites, determined by short sequence of nucleotide pairs. Restriction nucleases can therefore be used to produce a reproducible set specific DNA fragments from any genome. The main reason they are so useful is that a given enzyme will always cut a given DNA molecule at t ...
Macromolecule (biomolecule) Review Worksheet
... Sugars can be detected in foods through a simple lab test. To find out if a food contains starch, iodine (a reagent) is placed on the food. A food containing starch will turn black when in contact with iodine. A test for simple sugars involves mixing the food with a liquid blue reagent called Benedi ...
... Sugars can be detected in foods through a simple lab test. To find out if a food contains starch, iodine (a reagent) is placed on the food. A food containing starch will turn black when in contact with iodine. A test for simple sugars involves mixing the food with a liquid blue reagent called Benedi ...
Chapter 10: Biotechnology
... How can a DNA fingerprint be generated and how can it be used to identify an individual? • DNA fingerprinting reveals differences in the number of tandem repeats among different individuals’ DNA. • First, PCR is used to make many copies of a region of a chromosome known to have tandem repeats. • Th ...
... How can a DNA fingerprint be generated and how can it be used to identify an individual? • DNA fingerprinting reveals differences in the number of tandem repeats among different individuals’ DNA. • First, PCR is used to make many copies of a region of a chromosome known to have tandem repeats. • Th ...
gen-305-lect-14-2016
... - 1. It can determine if a specific protein is made in a particular cell type. Red blood cells vs. brain cells. - 2. It can determine if a specific protein is made at a particular stage of development. Fetal vs. adult cells Western blotting is carried out as such: - Proteins are extracted from the c ...
... - 1. It can determine if a specific protein is made in a particular cell type. Red blood cells vs. brain cells. - 2. It can determine if a specific protein is made at a particular stage of development. Fetal vs. adult cells Western blotting is carried out as such: - Proteins are extracted from the c ...
typing methods - Micro-Rao
... number and sizes of restriction fragments are influenced by the recognition sequence of enzyme and composition of DNA. Bacterial DNA is digested with endonucleases that have relatively frequent restriction sites, thereby generating hundreds of fragments ranging from ~0.5 to 50 kb in length. Such fra ...
... number and sizes of restriction fragments are influenced by the recognition sequence of enzyme and composition of DNA. Bacterial DNA is digested with endonucleases that have relatively frequent restriction sites, thereby generating hundreds of fragments ranging from ~0.5 to 50 kb in length. Such fra ...
Xian`s Southern Blot Protocol Using Digoxigenin Labeled Probe
... LABELING DNA PROBE USING DIG HIGH PRIME LABELING MIX (ROCHE) • dilute 10ng-3µg probe DNA (genomic, plasmid or gene clean fragment) in dsH2O to a final volume of 16µl • denature DNA by boiling 10min; quickly chill on ice to prevent reannealing of strands • add 4µl DIG high prime labeling mix; mix bri ...
... LABELING DNA PROBE USING DIG HIGH PRIME LABELING MIX (ROCHE) • dilute 10ng-3µg probe DNA (genomic, plasmid or gene clean fragment) in dsH2O to a final volume of 16µl • denature DNA by boiling 10min; quickly chill on ice to prevent reannealing of strands • add 4µl DIG high prime labeling mix; mix bri ...
Xian`s Southern Blot Protocol Using Digoxigenin Labeled Probe
... LABELING DNA PROBE USING DIG HIGH PRIME LABELING MIX (ROCHE) • dilute 10ng-3µg probe DNA (genomic, plasmid or gene clean fragment) in dsH2O to a final volume of 16µl • denature DNA by boiling 10min; quickly chill on ice to prevent reannealing of strands • add 4µl DIG high prime labeling mix; mix bri ...
... LABELING DNA PROBE USING DIG HIGH PRIME LABELING MIX (ROCHE) • dilute 10ng-3µg probe DNA (genomic, plasmid or gene clean fragment) in dsH2O to a final volume of 16µl • denature DNA by boiling 10min; quickly chill on ice to prevent reannealing of strands • add 4µl DIG high prime labeling mix; mix bri ...
DNA Puzzle
... In today’s lab you will use plastic puzzle pieces to do transcription and translation. The basic concepts we want you to learn are: Parts of a nucleotide Differences between DNA and RNA (KNOW THREE DIFFERENCES!!!!) Templates and complementary Strands Chargraff’s rules for pairs of nitrogenous bases ...
... In today’s lab you will use plastic puzzle pieces to do transcription and translation. The basic concepts we want you to learn are: Parts of a nucleotide Differences between DNA and RNA (KNOW THREE DIFFERENCES!!!!) Templates and complementary Strands Chargraff’s rules for pairs of nitrogenous bases ...
Agarose gel electrophoresis
![](https://commons.wikimedia.org/wiki/Special:FilePath/DNAgel4wiki.png?width=300)
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.Agarose gels are easy to cast and are particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7 - 2% dissolved in a suitable electrophoresis buffer.