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Bacterial Screening PCR Kit
... 5) After the gel has set, place it in an electrophoresis vat and add electrophoresis buffer to until the gel is completely immersed. 6) Gently remove the comb, taking care not to break the gel. 5. Electrophoresis 1) Connect the electrophoresis apparatus, taking care to connect the anode and cathcde ...
... 5) After the gel has set, place it in an electrophoresis vat and add electrophoresis buffer to until the gel is completely immersed. 6) Gently remove the comb, taking care not to break the gel. 5. Electrophoresis 1) Connect the electrophoresis apparatus, taking care to connect the anode and cathcde ...
Full-text - Association for Biology Laboratory Education
... students extract the enzyme that will be used in the assay has many merits, the data obtained from this method are often skewed or fluctuate from one run to the next which can be both confusing and frustrating to the introductory level student. I have found that performing a colorimetric assay using ...
... students extract the enzyme that will be used in the assay has many merits, the data obtained from this method are often skewed or fluctuate from one run to the next which can be both confusing and frustrating to the introductory level student. I have found that performing a colorimetric assay using ...
Materials and Methods
... g/mL aprotinin, 10 g/mL pepstatin, 10 g/mL TPCK and 10 g/mL TLCK). The extracts were sonicated on ice, the supernatant was obtained following a 5 min spin at 4 ºC in a microfuge at 13,000 rpm and the protein concentration was measured by the Bradford method. The proteins were then size-fractione ...
... g/mL aprotinin, 10 g/mL pepstatin, 10 g/mL TPCK and 10 g/mL TLCK). The extracts were sonicated on ice, the supernatant was obtained following a 5 min spin at 4 ºC in a microfuge at 13,000 rpm and the protein concentration was measured by the Bradford method. The proteins were then size-fractione ...
Lab Title
... inside the nucleus, and pretty much stays inside the nucleus, yet the proteins that DNA helps to make are produced OUTSIDE of the nucleus. So how does the cell solve this problem? It sends a “messenger” from the nucleus to the ribosomes in the cytoplasm. In a process called transcription, the DNA co ...
... inside the nucleus, and pretty much stays inside the nucleus, yet the proteins that DNA helps to make are produced OUTSIDE of the nucleus. So how does the cell solve this problem? It sends a “messenger” from the nucleus to the ribosomes in the cytoplasm. In a process called transcription, the DNA co ...
to Unit 10 Notes
... Introns – sequences in the DNA that are NOT used to make mRNA or to make a protein. They are NOT transcribed * Exons – sequences in the DNA that are expressed or used to make mRNA and and ultimately are used to make a protein ...
... Introns – sequences in the DNA that are NOT used to make mRNA or to make a protein. They are NOT transcribed * Exons – sequences in the DNA that are expressed or used to make mRNA and and ultimately are used to make a protein ...
Nucleic Acids B8
... condensation polymers (nucleic acids or polynucleotides). Living cells contain two different types of nucleic acids DNA (deoxyribose nucleic acid) – stores genetic info RNA (ribose nucleic acid) – protein synthesis Nucleic Acids are made up of Nucleotides which contain three smaller types of ...
... condensation polymers (nucleic acids or polynucleotides). Living cells contain two different types of nucleic acids DNA (deoxyribose nucleic acid) – stores genetic info RNA (ribose nucleic acid) – protein synthesis Nucleic Acids are made up of Nucleotides which contain three smaller types of ...
Replication 1
... Bidirectional replication involves two replication forks, which move in opposite directions ...
... Bidirectional replication involves two replication forks, which move in opposite directions ...
DNA2016 - saddlespace.org
... Primary source of genetic information RNA can be used in some cases ...
... Primary source of genetic information RNA can be used in some cases ...
Notes
... Despite this efficiency the DNA even for E. coli is quite long and as mentioned earlier requires scaffold proteins to package it into the cell. The drawings I usually do of a neat little circle sitting happily inside a cell may be a tad simplistic! BUT as mentioned earlier this is nothing compared t ...
... Despite this efficiency the DNA even for E. coli is quite long and as mentioned earlier requires scaffold proteins to package it into the cell. The drawings I usually do of a neat little circle sitting happily inside a cell may be a tad simplistic! BUT as mentioned earlier this is nothing compared t ...
Agarose gel electrophoresis
![](https://commons.wikimedia.org/wiki/Special:FilePath/DNAgel4wiki.png?width=300)
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.Agarose gels are easy to cast and are particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7 - 2% dissolved in a suitable electrophoresis buffer.