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Basic Biochemistry - Personal Webspace for QMUL
... The negative charge on ____________ becomes directly proportional to its mass -Mercaptoethanol is added to disrupt disulphide bonds if there are any present The proteins are now _____________________ Figure 3-7a, page 71 (3-7a, page 74) The protein mixture flows down the gel from the cath ...
... The negative charge on ____________ becomes directly proportional to its mass -Mercaptoethanol is added to disrupt disulphide bonds if there are any present The proteins are now _____________________ Figure 3-7a, page 71 (3-7a, page 74) The protein mixture flows down the gel from the cath ...
Plasmids
... The ends of the cut have an overhanging piece of single-stranded DNA. These are called "sticky ends" because they are able to base pair with any DNA molecule containing the complementary sticky end. In this case, both DNA preparations have complementary sticky ends and thus can pair with each other ...
... The ends of the cut have an overhanging piece of single-stranded DNA. These are called "sticky ends" because they are able to base pair with any DNA molecule containing the complementary sticky end. In this case, both DNA preparations have complementary sticky ends and thus can pair with each other ...
Phylogenetic analysis
... conserved genes across all organism are used in such studies. The most used sequences for phylogenetic and evolution studies have been those of ribosomal RNA (rRNA) which changes extremely slowly over time. It is the comparison of ribosomal RNA sequences from many organisms that led to a new classif ...
... conserved genes across all organism are used in such studies. The most used sequences for phylogenetic and evolution studies have been those of ribosomal RNA (rRNA) which changes extremely slowly over time. It is the comparison of ribosomal RNA sequences from many organisms that led to a new classif ...
mg-lecure1 - WordPress.com
... – In most animal cells, about 5% of the cytosine bases are methylated – More than 50% of the cytosine bases in some plants are methylated – No methylation of cytosine has been detected in yeast cells – very low levels of methylation (about 1 methylated cytosine base per 12,500 nucleotides) are found ...
... – In most animal cells, about 5% of the cytosine bases are methylated – More than 50% of the cytosine bases in some plants are methylated – No methylation of cytosine has been detected in yeast cells – very low levels of methylation (about 1 methylated cytosine base per 12,500 nucleotides) are found ...
Chapter 5
... 10. A gene is inserted into an ampicillin resistance gene in a plasmid. Will cells containing the resulting recombinant plasmid be sensitive or resistant to ampicillin? Answer: When inserted into a gene, the new DNA interrupts the previous gene. Thus, the antibiotic gene is unlikely to be functional ...
... 10. A gene is inserted into an ampicillin resistance gene in a plasmid. Will cells containing the resulting recombinant plasmid be sensitive or resistant to ampicillin? Answer: When inserted into a gene, the new DNA interrupts the previous gene. Thus, the antibiotic gene is unlikely to be functional ...
Chem 465 Biochemistry II Hour Exam 2
... soluble enzymes as a substrate so it can carry electrons from one enzyme to another. Involved in 2 electron reactions. Generally pass electrons on to Flavoproteins Flavoproteins - proteins that contain Flavin adenine dinucleotide. A flavin group attached to a sugar sometimes attached to an adenine n ...
... soluble enzymes as a substrate so it can carry electrons from one enzyme to another. Involved in 2 electron reactions. Generally pass electrons on to Flavoproteins Flavoproteins - proteins that contain Flavin adenine dinucleotide. A flavin group attached to a sugar sometimes attached to an adenine n ...
Southern Blotting and Related DNA Detection Techniques
... More recent modifications to Southern blotting The system shown in Figure 1 is an accurate description of Southern blotting as still carried out in many laboratories, but various modifications have been introduced over the years to improve the efficiency of DNA transfer from gel to membrane. The major ...
... More recent modifications to Southern blotting The system shown in Figure 1 is an accurate description of Southern blotting as still carried out in many laboratories, but various modifications have been introduced over the years to improve the efficiency of DNA transfer from gel to membrane. The major ...
Searching for Discriminant Fragments of
... Abstract: We collected more than 250 sequences of cytochrome c oxidase for species of the most orders of Hexapoda from Swiss-Prot protein knowledgebase. The discriminant fragments of cytochrome c oxidase at the order level were tentatively determined. We compared the frequency distributions of diffe ...
... Abstract: We collected more than 250 sequences of cytochrome c oxidase for species of the most orders of Hexapoda from Swiss-Prot protein knowledgebase. The discriminant fragments of cytochrome c oxidase at the order level were tentatively determined. We compared the frequency distributions of diffe ...
Chapter 8 Nucleotides and Nucleic acids
... heat up or change pH goes though transition where loses viscosity sharply just like denaturation of protein, are denaturing DNA disrupting H bonds between bases and base stacking interaction two strand unwind and can separate NO BONDS BROKEN If a strand haven’t complex separated, can anneal quickly ...
... heat up or change pH goes though transition where loses viscosity sharply just like denaturation of protein, are denaturing DNA disrupting H bonds between bases and base stacking interaction two strand unwind and can separate NO BONDS BROKEN If a strand haven’t complex separated, can anneal quickly ...
Nucleic Acid structure - part 1
... 1. Base composition of DNA varies from one species to another 2. DNA from different tissues of same species have same base composition 3. Base composition of DNA in given species does not change with age, nutritional state, environment 4. In all cellular DNAs, regardless of species, the number of ad ...
... 1. Base composition of DNA varies from one species to another 2. DNA from different tissues of same species have same base composition 3. Base composition of DNA in given species does not change with age, nutritional state, environment 4. In all cellular DNAs, regardless of species, the number of ad ...
Nucleic Acid structure
... 1. Base composition of DNA varies from one species to another 2. DNA from different tissues of same species have same base composition 3. Base composition of DNA in given species does not change with age, nutritional state, environment 4. In all cellular DNAs, regardless of species, the number of ad ...
... 1. Base composition of DNA varies from one species to another 2. DNA from different tissues of same species have same base composition 3. Base composition of DNA in given species does not change with age, nutritional state, environment 4. In all cellular DNAs, regardless of species, the number of ad ...
RNA:Structure, Function, Transcription, Translation
... a. What are the four nitrogen bases used to make RNA nucleotides? ...
... a. What are the four nitrogen bases used to make RNA nucleotides? ...
demonstating sequence-specific cleavage by a restriction enzyme
... Page 23 mac85 Mac 85:1st shift: 1268_tm:8945d: ...
... Page 23 mac85 Mac 85:1st shift: 1268_tm:8945d: ...
Agarose gel electrophoresis
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Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.Agarose gels are easy to cast and are particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7 - 2% dissolved in a suitable electrophoresis buffer.