biologi eksam quetion summary
... The same enzyme then cuts out a part of the vector ( or “sticky ends” are added after) This gives “complimentary sticky ends” that can attach to each other by base pairing Nicks in the strand are then healed by ligase, forms the phosphodiester bonds The gene can then be cloned in the vector ...
... The same enzyme then cuts out a part of the vector ( or “sticky ends” are added after) This gives “complimentary sticky ends” that can attach to each other by base pairing Nicks in the strand are then healed by ligase, forms the phosphodiester bonds The gene can then be cloned in the vector ...
DNA Structure and Replication
... -Was determined by Watson and Crick based on an x-ray by Rosalind Franklin -Double helix made of deoxyribose and phosphate backbone; nitrogen bases make up the rungs -A-T are held together by 2 H bonds -C-G are held together by 3 H bonds -Strands are complementary which provides a mechanism for repl ...
... -Was determined by Watson and Crick based on an x-ray by Rosalind Franklin -Double helix made of deoxyribose and phosphate backbone; nitrogen bases make up the rungs -A-T are held together by 2 H bonds -C-G are held together by 3 H bonds -Strands are complementary which provides a mechanism for repl ...
Introduction - OpenWetWare
... ligases can join the ends of two different DNA fragments, or join the two ends of a single fragment. There are two types of ligation, called sticky-end ligation and blunt-end ligation, whose names refer to the presence or absence of short overhangs on the ends of the DNA being ligated. Blunt-end lig ...
... ligases can join the ends of two different DNA fragments, or join the two ends of a single fragment. There are two types of ligation, called sticky-end ligation and blunt-end ligation, whose names refer to the presence or absence of short overhangs on the ends of the DNA being ligated. Blunt-end lig ...
Rapid Method for Extraction of Genomic DNA From Vitex negundo L.
... Studies were undertaken for detection of DNA polymorphism through Random Amplified Polymorphic (RAPD) DNA fingerprint in Plumbago zeylanica L. collected from ten locations in Western Ghats. A total of 27 DNA fragment (bands), ranging from 4 to 6 was observed. The genetic distance between the populat ...
... Studies were undertaken for detection of DNA polymorphism through Random Amplified Polymorphic (RAPD) DNA fingerprint in Plumbago zeylanica L. collected from ten locations in Western Ghats. A total of 27 DNA fragment (bands), ranging from 4 to 6 was observed. The genetic distance between the populat ...
AP Biology Double helix structure of DNA
... Replication of DNA base pairing allows each strand to serve as a template for a ...
... Replication of DNA base pairing allows each strand to serve as a template for a ...
Brouwer_791H_Proposal - University of New Hampshire
... electrons hit the ring, it induces a current proportional to the amount of electrons deflected. The result is a map of two dimensional positions versus current (intensity), in which the brightest spots mark the largest atoms and will be the focus of our attention. This new technique is useful becaus ...
... electrons hit the ring, it induces a current proportional to the amount of electrons deflected. The result is a map of two dimensional positions versus current (intensity), in which the brightest spots mark the largest atoms and will be the focus of our attention. This new technique is useful becaus ...
16Discovery Of DNA
... number of adenines was approximately equal to the number of thymines (%T = %A). • The number of guanines was approximately equal to the number of cytosines (%G = %C). ...
... number of adenines was approximately equal to the number of thymines (%T = %A). • The number of guanines was approximately equal to the number of cytosines (%G = %C). ...
Bioinstrumentation - E
... 8) The matrices commonly used in column chromatography are ______ (1) Dextran (2) Cellulose (3) Polystyrene (4) All 9) Which of the following elution technique is used in column chromatography? (1) Isocratic (2) Batch (3) Gradient (4) All 10) _______ is a cation exchanger. (1) CM-Sephadex (2) Polyst ...
... 8) The matrices commonly used in column chromatography are ______ (1) Dextran (2) Cellulose (3) Polystyrene (4) All 9) Which of the following elution technique is used in column chromatography? (1) Isocratic (2) Batch (3) Gradient (4) All 10) _______ is a cation exchanger. (1) CM-Sephadex (2) Polyst ...
Techniques of Protein and Nucleic Acid Purification
... Only the desired protein(s) in an impure mixture will bind to the matrix Protein can be eluted in pure form by altering conditions, e.g. by adding large amount of free ligand ...
... Only the desired protein(s) in an impure mixture will bind to the matrix Protein can be eluted in pure form by altering conditions, e.g. by adding large amount of free ligand ...
DNA barcoding in medicinal plants: Testing the potential of a
... titled "Biological identifications through DNA barcodes". ...
... titled "Biological identifications through DNA barcodes". ...
Agarose gel electrophoresis
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.Agarose gels are easy to cast and are particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7 - 2% dissolved in a suitable electrophoresis buffer.