DNA Sequences Analysis
... A DNA sequencing printout. The sequence is represented by a series of peaks, one for each nucleotide position. In this example, a red peak is an A, blue is a C, orange is a G, and green is a T. ...
... A DNA sequencing printout. The sequence is represented by a series of peaks, one for each nucleotide position. In this example, a red peak is an A, blue is a C, orange is a G, and green is a T. ...
Caffeine Metabolism Gene Zephyr and Walsh (2015)
... asked to identify the restriction sites for both ApaI and SacI, describe the sizes that will occur when the enzymes do or do not cut, and then draw out the gels for homozygotes and a heterozygote. In this way, students can form an educated guess about the variety of results they may achieve and make ...
... asked to identify the restriction sites for both ApaI and SacI, describe the sizes that will occur when the enzymes do or do not cut, and then draw out the gels for homozygotes and a heterozygote. In this way, students can form an educated guess about the variety of results they may achieve and make ...
Recombinant DNA Technology - BLI-Research-Synbio
... over blunt end cutters because DNA fragments can be joined easily together. • When DNA from two sources is joined together, the enzyme DNA ligase is used to catalyze bonding between sugar and phosphate groups in the DNA backbone. • DNA from a “foreign” source (plant, animal, viral, bacterial, yeast) ...
... over blunt end cutters because DNA fragments can be joined easily together. • When DNA from two sources is joined together, the enzyme DNA ligase is used to catalyze bonding between sugar and phosphate groups in the DNA backbone. • DNA from a “foreign” source (plant, animal, viral, bacterial, yeast) ...
Lesson Overview
... replicated, because each base on one strand pairs with only one base on the opposite strand. Each strand of the double helix has all the information needed to reconstruct the other half by the mechanism of base pairing. Because each strand can be used to make the other strand, the strands are said t ...
... replicated, because each base on one strand pairs with only one base on the opposite strand. Each strand of the double helix has all the information needed to reconstruct the other half by the mechanism of base pairing. Because each strand can be used to make the other strand, the strands are said t ...
What are enzymes and how do they work
... 5. Then imagine that the test tube was cooled to about 60°C, a temperature at which hydrogen bonds can reform. Green, blue and black molecules can bind in two distinct ways. Sketch each below. (It is CRUCIAL that you use appropriate colors to indicate different molecules at this point.) OR ...
... 5. Then imagine that the test tube was cooled to about 60°C, a temperature at which hydrogen bonds can reform. Green, blue and black molecules can bind in two distinct ways. Sketch each below. (It is CRUCIAL that you use appropriate colors to indicate different molecules at this point.) OR ...
Transcription 12.06.21 lec
... Transcription. Here's a strand of DNA that gets split apart; then there's the initial copying of that chain. Transcription is where you're just making pieces of RNA from DNA. Translation's where you act ...
... Transcription. Here's a strand of DNA that gets split apart; then there's the initial copying of that chain. Transcription is where you're just making pieces of RNA from DNA. Translation's where you act ...
recombinant DNA. Lesson Overview
... It is relatively easy to extract DNA from cells and tissues. The extracted DNA can be cut into fragments of manageable size using restriction enzymes These restriction fragments can then be separated according to size, using gel electrophoresis or another similar technique. ...
... It is relatively easy to extract DNA from cells and tissues. The extracted DNA can be cut into fragments of manageable size using restriction enzymes These restriction fragments can then be separated according to size, using gel electrophoresis or another similar technique. ...
PCR: Polymerase Chain Reaction
... something found in such small amounts that without PCR it would be undetectable. ...
... something found in such small amounts that without PCR it would be undetectable. ...
B3.3 Genetics ANSWERS Worksheet Two Molecular Genetics 1
... transcription only uses the coding strand. The enzymes are also different; DNA replication uses helicase, DNA polymerase and DNA ligase, whereas transcription uses RNA polymerase. ...
... transcription only uses the coding strand. The enzymes are also different; DNA replication uses helicase, DNA polymerase and DNA ligase, whereas transcription uses RNA polymerase. ...
Genetics 314 – Spring 2004
... This is a case of a eukaryotic gene that does not have any introns. So when the gene is transcribed and translated in a prokaryote the message has no extra bases resulting in a polypeptide that does hot have any extra amino acids. Extra credit PCR (polymerase chain reaction) is a method to rapidly r ...
... This is a case of a eukaryotic gene that does not have any introns. So when the gene is transcribed and translated in a prokaryote the message has no extra bases resulting in a polypeptide that does hot have any extra amino acids. Extra credit PCR (polymerase chain reaction) is a method to rapidly r ...
Supplementary Methods
... uridine (U), according to standard solid phase oligonucleotide synthesis protocols1. For antagomirs. i.e., cholesterol conjugated RNAs, the synthesis started from a controlledpore glass solid support carrying a cholesterol- hydroxyprolinol linker2. Antagomirs with phosphorothioate backbone at a give ...
... uridine (U), according to standard solid phase oligonucleotide synthesis protocols1. For antagomirs. i.e., cholesterol conjugated RNAs, the synthesis started from a controlledpore glass solid support carrying a cholesterol- hydroxyprolinol linker2. Antagomirs with phosphorothioate backbone at a give ...
Student Handout Hands-on Activity HIV Reverse Transcription and
... rather than a terminal illness. The drug azidothymidine (AZT) is one of the drugs commonly used in this drug cocktail. AZT targets a critical step in the HIV replication cycle: reverse transcription. HIV has a single-stranded RNA genome. During reverse transcription an HIV enzyme converts the HIV ...
... rather than a terminal illness. The drug azidothymidine (AZT) is one of the drugs commonly used in this drug cocktail. AZT targets a critical step in the HIV replication cycle: reverse transcription. HIV has a single-stranded RNA genome. During reverse transcription an HIV enzyme converts the HIV ...
Tth RecA
... between 65–75°C. The extreme thermostability makes Tth RecA ideal for molecular biology applications that require an elevated temperature condition, such as nucleic acid amplification and sequencing. Highlights Extreme thermostability Nucleic acid amplification and sequencing Isolated from a recombi ...
... between 65–75°C. The extreme thermostability makes Tth RecA ideal for molecular biology applications that require an elevated temperature condition, such as nucleic acid amplification and sequencing. Highlights Extreme thermostability Nucleic acid amplification and sequencing Isolated from a recombi ...
Agarose gel electrophoresis
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.Agarose gels are easy to cast and are particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7 - 2% dissolved in a suitable electrophoresis buffer.