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dna technology and genomics
dna technology and genomics

... 9) Describe PCR and explain the advantages and limitations of this procedure. 10) Explain how gel electrophoresis is used to analyze nucleic acids and to distinguish between two alleles of a gene. 11) Describe the process of nucleic acid hybridization. 12) Describe the Southern blotting procedure an ...
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... individual genome include next-generation sequencing methods and DNA microarrays. A DNA microarray detects DNA or RNA sequences that are complementary to and hybridize with an oligonucleotide probe. The aim is to find out which SNPs are associated with specific diseases and identify alleles that con ...
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... individual genome include next-generation sequencing methods and DNA microarrays. A DNA microarray detects DNA or RNA sequences that are complementary to and hybridize with an oligonucleotide probe. The aim is to find out which SNPs are associated with specific diseases and identify alleles that con ...
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... Each point represents a unique clone in this loglog scatter plot. The points in red are found in both repetitions, while those in blue are only found in one repetition. C) Sequence copy counts obtained by sequencing two separate PCR reactions performed using template from the same DNA extraction. ...
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Genotyping the Exome of the Black Cottonwood Tree
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... DNA Sequencing Advances in DNA Sequencing Part I Overview  Maxam Gilbert Sequencing-(1976)  Sanger Sequencing Sequencing has been used to map the human genomes, mutation detection study genetic diseases such as CF, MS and to study cancer susceptibility genes. Fluoresent technology In 1986 ABI dev ...
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Whole genome sequencing



Whole genome sequencing (also known as full genome sequencing, complete genome sequencing, or entire genome sequencing) is a laboratory process that determines the complete DNA sequence of an organism's genome at a single time. This entails sequencing all of an organism's chromosomal DNA as well as DNA contained in the mitochondria and, for plants, in the chloroplast.Whole genome sequencing should not be confused with DNA profiling, which only determines the likelihood that genetic material came from a particular individual or group, and does not contain additional information on genetic relationships, origin or susceptibility to specific diseases. Also unlike full genome sequencing, SNP genotyping covers less than 0.1% of the genome. Almost all truly complete genomes are of microbes; the term ""full genome"" is thus sometimes used loosely to mean ""greater than 95%"". The remainder of this article focuses on nearly complete human genomes.High-throughput genome sequencing technologies have largely been used as a research tool and are currently being introduced in the clinics. In the future of personalized medicine, whole genome sequence data will be an important tool to guide therapeutic intervention. The tool of gene sequencing at SNP level is also used to pinpoint functional variants from association studies and improve the knowledge available to researchers interested in evolutionary biology, and hence may lay the foundation for predicting disease susceptibility and drug response.
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