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Next-Generation Sequencing: an overview of technologies and
Next-Generation Sequencing: an overview of technologies and

... Human Genome Project • Launched in 1989 –expected to take 15 years – Competing Celera project launched in 1998 • Genome estimated to be 92% complete – 1st Draft released in 2000 – “Complete” genome released in 2003 – Sequence of last chromosome published in ...


... DNA Profiling • DNA profiling allows the identification of individuals by comparing regions of the genome with highly variable number of repetitive sequences of DNA. • This can be used forensically, for example to identify criminals and in paternity testing, or medically, for example to analyse dis ...
STSE Power point
STSE Power point

... Also examining relationships between different diseases Further studies may include inflammatory arthritis, Respiratory, Infectious and dermatological diseases ...
Chromosomes
Chromosomes

... Conflict between male and female over allocation of maternal resources to offspring Paternally expressed genes would promote growth, maternally expressed genes should slow it down. ...
Mitochondrial - Reversible infantile respiratory chain deficiency
Mitochondrial - Reversible infantile respiratory chain deficiency

... o Prenatal referrals are only accepted from Clinical Genetics and / or Prenatal Diagnosis. They must be discussed with the laboratory and arranged in advance. STRATEGY o Genetic testing is directed by the clinical phenotype. o Typically analysis for the m.14674T>C/G mitochondrial DNA mutation is und ...
Transposons: Mobile DNA DNA
Transposons: Mobile DNA DNA

... 1. An RNA copy of transposon is synthesized by the normal process of transcription. 2. The RNA transcript is copied into DNA, which initially exists as an independent molecule outside of the genome. This conversion of RNA to DNA, the reverse of normal transcription process required reverse transcrip ...
Lecture
Lecture

... Homolog– genes sharing a common origin note: two genes are homologs or they or not no such thing as %homology or “more homologous” ...
7529 DNA Sequencing - ACM
7529 DNA Sequencing - ACM

... sequencing. Their first customer is Sandy, the squirrel scientist, who has found the corpse of an alien from the outer space and asked Krusty-Royan crew to extract its DNA sequence. Contrary to the life on earth, the DNA of the alien was not only composed of the 4 well-known nucleotides (A, C, G, an ...
BB30055: Genes and genomes
BB30055: Genes and genomes

... 3) SNPs (single nucleotide polymorphisms) Point mutations in single base pairs > 1.4million SNPs identified (~ 1 in every 1.9kb length on average)  ~60,000 SNPs lie within exons and untranslated regions (85% of exons lie within 5kb of a SNP)  May or may not affect the ORF (synonymous or non synon ...
Document
Document

... * 1986 Leroy E. Hood's laboratory at the California Institute of Technology and Smith announce the first semi-automated DNA sequencing machine. * 1987 Applied Biosystems markets first automated sequencing machine, the model ABI 370. * 1990 The U.S. National Institutes of Health (NIH) begins large-sc ...
One system, one workflow, powerful new sequencing applications
One system, one workflow, powerful new sequencing applications

... The Chromium Genome provides long range information on a genome-wide scale, including variant calling, phasing and extensive characterization of genomic structure. Unlock critical genetic information for variants in heritable disorders, and discover key alterations in cancer. ...
AP Ch 19
AP Ch 19

... 3 Sequence each fragment. ...
Classification of DNA sequences using Bloom Filters
Classification of DNA sequences using Bloom Filters

... Stockholm, SWEDEN ...
GenomeBrowser - CBS
GenomeBrowser - CBS

... • Less easy to use • Maintained/updated by several people ...
Synthetic Life - Colin Mayfield
Synthetic Life - Colin Mayfield

... • M. mycoides JCVI-syn1.0 was transformed with a vector containing a selectable tetracycline-resistance marker and a b-galactosidase gene for screening • PCR experiments and Southern blot analysis of isolated putative transplanted cells • Multiple specific antibody reactions were carried out to test ...
Plant transposons
Plant transposons

... development results in smaller sectors. ...
Slide 1
Slide 1

... Targeted sequencing is a powerful tool for assessing the organisms that are present in microbial communities, but it is limited in terms of the functional and genetic information produced. Organisms for which the genome sequences are known (currently there are several thousand sequenced bacterial ge ...
here - Triticeae CAP
here - Triticeae CAP

... overlap between BACs, thus providing a complete secharacterized. The genotype controls the way an organquence that has a physical relationship and can be anism looks, which is called the phenotype. In our analogy, ...
Whose DNA was sequenced for the Human Genome Project?
Whose DNA was sequenced for the Human Genome Project?

... has resulted in several notable improvements to the HGP. An example is the decision to sequence the DNA of several anonymous individuals, rather than a known individual, in order to protect privacy. Another example is the development of widely used genetic privacy guidelines and draft legislation. T ...
Genetic Testing in Primary Care - Genetics in Primary Care Institute
Genetic Testing in Primary Care - Genetics in Primary Care Institute

... disorders can affect many genes at once and can vary significantly in size. Some dosage disorders are caused by “gene inactivation.” With inactivation, the genetic material is present, however, it has been inactivated through a chemical process called methylation and therefore, that genetic material ...
Lars Steinmetz, Wolfgang Huber, Richard Bourgon and
Lars Steinmetz, Wolfgang Huber, Richard Bourgon and

... genome, and that such information will prove useful in understanding how disease genes are passed from generation to generation. To date, around 2000 disease genes have been identified in humans which are inherited in simple, Mendelian fashion, and a few hundred that contribute in more complex ways ...
Chapter 1 - Test bank for TextBook
Chapter 1 - Test bank for TextBook

... genomics, and offers examples of DNA information impacting daily life. In this new era of genomics, individuals have access to their own genetic information, and health care providers are learning how to incorporate DNA data into diagnostic and therapeutic medicine. Bioethics deals with issues of pr ...
Apple Molecular Biology: Animation 1
Apple Molecular Biology: Animation 1

... The apple is very important to the world economy and food supply. Cultivated since the first century, apples are an ideal candidate for genomic study. Information gained from researching an important crop species such as apple, contributes the overall general knowledge as well as allowing researcher ...
The rhesus macaque is the third primate genome to be completed
The rhesus macaque is the third primate genome to be completed

... Here's the key: Six million years isn't long in evolutionary history. So if a particular gene is different in the human and the chimp, it's impossible to know which version came first. ...
Slide 1
Slide 1

... relationships, ChIP-chip data ... • Based on experimental data and computational ...
< 1 ... 70 71 72 73 74 75 76 77 78 ... 115 >

Whole genome sequencing



Whole genome sequencing (also known as full genome sequencing, complete genome sequencing, or entire genome sequencing) is a laboratory process that determines the complete DNA sequence of an organism's genome at a single time. This entails sequencing all of an organism's chromosomal DNA as well as DNA contained in the mitochondria and, for plants, in the chloroplast.Whole genome sequencing should not be confused with DNA profiling, which only determines the likelihood that genetic material came from a particular individual or group, and does not contain additional information on genetic relationships, origin or susceptibility to specific diseases. Also unlike full genome sequencing, SNP genotyping covers less than 0.1% of the genome. Almost all truly complete genomes are of microbes; the term ""full genome"" is thus sometimes used loosely to mean ""greater than 95%"". The remainder of this article focuses on nearly complete human genomes.High-throughput genome sequencing technologies have largely been used as a research tool and are currently being introduced in the clinics. In the future of personalized medicine, whole genome sequence data will be an important tool to guide therapeutic intervention. The tool of gene sequencing at SNP level is also used to pinpoint functional variants from association studies and improve the knowledge available to researchers interested in evolutionary biology, and hence may lay the foundation for predicting disease susceptibility and drug response.
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