Pfu DNA Polymerase - G
... has superior thermostability and proofreading properties compared to the other thermostable polymerase. Its molecular weight is 90 kD. It can amplify DNA target up to 2kb. The elongation velocity is 0.2~0.4kb/min (70~75°C). Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that ...
... has superior thermostability and proofreading properties compared to the other thermostable polymerase. Its molecular weight is 90 kD. It can amplify DNA target up to 2kb. The elongation velocity is 0.2~0.4kb/min (70~75°C). Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that ...
What is a southern blot?
... disease, gene on β - globin S allele is the transforms an SSA To replace T in the sixth codon “A” poicion 2 β. Southern blot analysis detected the presence of DNA in a complex mixture, for this the techniques described above are used to complete the probe hbridacion and get the sought. ...
... disease, gene on β - globin S allele is the transforms an SSA To replace T in the sixth codon “A” poicion 2 β. Southern blot analysis detected the presence of DNA in a complex mixture, for this the techniques described above are used to complete the probe hbridacion and get the sought. ...
Restriction Enzymes
... • Restriction enzymes are endonucleases which catalyze the cleavage of the phosphodiester bonds within both strands of DNA. • They require Mg+2 for activity and generate a 5 prime (5') phosphate and a 3 prime (3') hydroxyl group at the point of cleavage. • The distinguishing feature of restriction e ...
... • Restriction enzymes are endonucleases which catalyze the cleavage of the phosphodiester bonds within both strands of DNA. • They require Mg+2 for activity and generate a 5 prime (5') phosphate and a 3 prime (3') hydroxyl group at the point of cleavage. • The distinguishing feature of restriction e ...
Gel Electrophoresis
... Ethidium bromide – a DNA stain (indicator); glows orange when it is mixed with DNA and exposed to UV light; abbreviated EtBr • Used to see how molecules were separated after running gel ...
... Ethidium bromide – a DNA stain (indicator); glows orange when it is mixed with DNA and exposed to UV light; abbreviated EtBr • Used to see how molecules were separated after running gel ...
Gel Electrophoresis
... You had to grow tons and tons of tiny cells. Then along came a guy named Dr. Kary Mullis, Said you can amplify in vitro just as well. Just mix your template with a buffer and some primers, Nucleotides and polymerases, too. Denaturing, annealing, and extending. Well it’s amazing what heating and cool ...
... You had to grow tons and tons of tiny cells. Then along came a guy named Dr. Kary Mullis, Said you can amplify in vitro just as well. Just mix your template with a buffer and some primers, Nucleotides and polymerases, too. Denaturing, annealing, and extending. Well it’s amazing what heating and cool ...
DNA Sequences Analysis
... • DNA consists of two long interwoven strands that form the famous “double helix”. Each Strand is built from a small set of molecules called nucleotides. • Often the length of double-stranded DNA is expressed in the units of basepairs (bp), kilobasepairs (kb), or megabasepairs (Mb), so that this siz ...
... • DNA consists of two long interwoven strands that form the famous “double helix”. Each Strand is built from a small set of molecules called nucleotides. • Often the length of double-stranded DNA is expressed in the units of basepairs (bp), kilobasepairs (kb), or megabasepairs (Mb), so that this siz ...
Maurice Wilkins
Maurice Hugh Frederick Wilkins CBE FRS (15 December 1916 – 5 October 2004) was a New Zealand-born English physicist and molecular biologist, and Nobel Laureate whose research contributed to the scientific understanding of phosphorescence, isotope separation, optical microscopy and X-ray diffraction, and to the development of radar. He is best known for his work at King's College, London on the structure of DNA which falls into three distinct phases. The first was in 1948–50 where his initial studies produced the first clear X-ray images of DNA which he presented at a conference in Naples in 1951 attended by James Watson. During the second phase of work (1951–52) he produced clear ""B form"" ""X"" shaped images from squid sperm which he sent to James Watson and Francis Crick causing Watson to write ""Wilkins... has obtained extremely excellent X-ray diffraction photographs""[of DNA]. Throughout this period Wilkins was consistent in his belief that DNA was helical even when Rosalind Franklin expressed strong views to the contrary.In 1953 Franklin instructed Raymond Gosling to give Wilkins, without condition, a high quality image of ""B"" form DNA which she had unexpectedly produced months earlier but had “put it aside” to concentrate on other work. Wilkins, having checked that he was free to personally use the photograph to confirm his earlier results, showed it to Watson without the consent of Rosalind Franklin. This image, along with the knowledge that Linus Pauling had published an incorrect structure of DNA, “mobilised” Watson to restart model building efforts with Crick. Important contributions and data from Wilkins, Franklin (obtained via Max Perutz) and colleagues in Cambridge enabled Watson and Crick to propose a double-helix model for DNA. The third and longest phase of Wilkins' work on DNA took place from 1953 onwards. Here Wilkins led a major project at King's College, London, to test, verify and make significant corrections to the DNA model proposed by Watson and Crick and to study the structure of RNA. Wilkins, Crick and Watson were awarded the 1962 Nobel Prize for Physiology or Medicine, ""for their discoveries concerning the molecular structure of nucleic acids and its significance for information transfer in living material.""