Identification of Different Meat Species by the Agilent Fish ID
... tissue samples using the Agilent DNA Isolation kit provided as part of the Fish ID solution. Two independent DNA samples for each of the meat tissues were used in the PCR together with the salmon positive control DNA supplied by the kit and a no template control. The results of the PCR reactions wer ...
... tissue samples using the Agilent DNA Isolation kit provided as part of the Fish ID solution. Two independent DNA samples for each of the meat tissues were used in the PCR together with the salmon positive control DNA supplied by the kit and a no template control. The results of the PCR reactions wer ...
Restriction Enzymes - mvhs
... Restriction Enzymes: Molecular Scissors • Restriction enzymes (endonuleases) cut DNA at specific sequences • What kinds of bonds are broken when restriction enzymes cut? – Covalent bonds (within a single strand) – Hydrogen bonds (between Hydrogen strands) as a result of the bond Covalent bond stran ...
... Restriction Enzymes: Molecular Scissors • Restriction enzymes (endonuleases) cut DNA at specific sequences • What kinds of bonds are broken when restriction enzymes cut? – Covalent bonds (within a single strand) – Hydrogen bonds (between Hydrogen strands) as a result of the bond Covalent bond stran ...
DNA Analysis
... • Alphabet of bases = {A,T,C,G} not uniformly distributed in any sequence and the composition vary within and between sequences ...
... • Alphabet of bases = {A,T,C,G} not uniformly distributed in any sequence and the composition vary within and between sequences ...
Human Genomics
... Human Genome Project • Started in 1986 (USA and UK) but officially ‘started’ in 1990, Europe and Japan joined in 1992 • Completed in 2003 – under budget and 2 years early • The sequence is not that of one person, but is a composite derived from several individuals. Therefore, it is a "representativ ...
... Human Genome Project • Started in 1986 (USA and UK) but officially ‘started’ in 1990, Europe and Japan joined in 1992 • Completed in 2003 – under budget and 2 years early • The sequence is not that of one person, but is a composite derived from several individuals. Therefore, it is a "representativ ...
A modified acidic approach for DNA extraction from
... agarose gels, PCR using specific microsatellite markers is frequently unsuccessful. A previous study on this species reported poor PCR amplification using RAPD markers because of the co-extraction of polyphenols during DNA isolation (Silva et al., 2006). In addition to the CTAB method (Doyle and Doy ...
... agarose gels, PCR using specific microsatellite markers is frequently unsuccessful. A previous study on this species reported poor PCR amplification using RAPD markers because of the co-extraction of polyphenols during DNA isolation (Silva et al., 2006). In addition to the CTAB method (Doyle and Doy ...
PCR: Polymerase Chain Reaction
... Add single DNA nucleotides and an enzyme that reads opposing strains sequence and extend primers sequence to match (complementary). Taq polymerase is used because it can withstand high temperature necessary for DNA strand separation and can be left in reaction to attach and start copying the templat ...
... Add single DNA nucleotides and an enzyme that reads opposing strains sequence and extend primers sequence to match (complementary). Taq polymerase is used because it can withstand high temperature necessary for DNA strand separation and can be left in reaction to attach and start copying the templat ...
(DNA) polymerase I
... 2. Draw a labelled diagram to show four DNA nucleotides, each with a different base, linked ...
... 2. Draw a labelled diagram to show four DNA nucleotides, each with a different base, linked ...
BT_Ch4_Presentation
... an adenosine bonded to a thymine or a gaunine bonded to a cytosine Phosphodiester bond – a bond that is responsible for polymerization of nucleic acids by linking sugars and phosphates of adjacent nucleotides Hydrogen bond – a type of weak bond that involved the “sandwiching” of a hydrogen atom betw ...
... an adenosine bonded to a thymine or a gaunine bonded to a cytosine Phosphodiester bond – a bond that is responsible for polymerization of nucleic acids by linking sugars and phosphates of adjacent nucleotides Hydrogen bond – a type of weak bond that involved the “sandwiching” of a hydrogen atom betw ...
Chapter 10 Manipulating Genes
... Rare Cellular Proteins Can Be Made in Large Amounts Using Cloned DNA Thousands of different proteins in a eukaryotic cell, including many with crucially important functions, are present in very small amounts. For these, it used to be extremely difficult, if not impossible, more than a few micrograms ...
... Rare Cellular Proteins Can Be Made in Large Amounts Using Cloned DNA Thousands of different proteins in a eukaryotic cell, including many with crucially important functions, are present in very small amounts. For these, it used to be extremely difficult, if not impossible, more than a few micrograms ...
Plasmids and DNA Digestion
... Restriction Enzyme: Special type of enzyme which “cuts” DNA, breaking the double helix. Restriction enzymes recognize target DNA sequences (usually palindromic). Agarose: A type of carbohydrate (sugar). Agarose is frequently polymerized into long chains to form gels that are suitable for DNA electro ...
... Restriction Enzyme: Special type of enzyme which “cuts” DNA, breaking the double helix. Restriction enzymes recognize target DNA sequences (usually palindromic). Agarose: A type of carbohydrate (sugar). Agarose is frequently polymerized into long chains to form gels that are suitable for DNA electro ...
Revised 2015 15.2 PowerPoint
... One of the first uses of transgenesis was to make the E. Coli bacteria produce human insulin, which could then be gathered cheaply, rather than having to be harvested from more expensive animals like pigs. A more contemporary example can be seen in the use of transgenic goats to product an anticoagu ...
... One of the first uses of transgenesis was to make the E. Coli bacteria produce human insulin, which could then be gathered cheaply, rather than having to be harvested from more expensive animals like pigs. A more contemporary example can be seen in the use of transgenic goats to product an anticoagu ...
Student Handout Hands-on Activity HIV Reverse Transcription and
... Figure 3. Two illustrations of DNA nucleotide chains. The left side of the figure shows the chemical structure of a DNA sequence that is three nucleotides long. The 5’ end has a free phosphate group (attached to the 5’ carbon of the ribose), and the 3’ end has a free hydroxyl (OH) group. When a ne ...
... Figure 3. Two illustrations of DNA nucleotide chains. The left side of the figure shows the chemical structure of a DNA sequence that is three nucleotides long. The 5’ end has a free phosphate group (attached to the 5’ carbon of the ribose), and the 3’ end has a free hydroxyl (OH) group. When a ne ...
Online Counseling Resource YCMOU ELearning Drive…
... Pol δ: main polymerase on the lagging strand, it is highly processive and has 3'->5' exonuclease activity. Pol ε: primary leading strand DNA polymerase, and also highly processive and has 3'->5' exonuclease activity. η, ι, κ, and Rev1 are Y-family DNA polymerases and Pol ζ is a B-family DNA po ...
... Pol δ: main polymerase on the lagging strand, it is highly processive and has 3'->5' exonuclease activity. Pol ε: primary leading strand DNA polymerase, and also highly processive and has 3'->5' exonuclease activity. η, ι, κ, and Rev1 are Y-family DNA polymerases and Pol ζ is a B-family DNA po ...
Maurice Wilkins
Maurice Hugh Frederick Wilkins CBE FRS (15 December 1916 – 5 October 2004) was a New Zealand-born English physicist and molecular biologist, and Nobel Laureate whose research contributed to the scientific understanding of phosphorescence, isotope separation, optical microscopy and X-ray diffraction, and to the development of radar. He is best known for his work at King's College, London on the structure of DNA which falls into three distinct phases. The first was in 1948–50 where his initial studies produced the first clear X-ray images of DNA which he presented at a conference in Naples in 1951 attended by James Watson. During the second phase of work (1951–52) he produced clear ""B form"" ""X"" shaped images from squid sperm which he sent to James Watson and Francis Crick causing Watson to write ""Wilkins... has obtained extremely excellent X-ray diffraction photographs""[of DNA]. Throughout this period Wilkins was consistent in his belief that DNA was helical even when Rosalind Franklin expressed strong views to the contrary.In 1953 Franklin instructed Raymond Gosling to give Wilkins, without condition, a high quality image of ""B"" form DNA which she had unexpectedly produced months earlier but had “put it aside” to concentrate on other work. Wilkins, having checked that he was free to personally use the photograph to confirm his earlier results, showed it to Watson without the consent of Rosalind Franklin. This image, along with the knowledge that Linus Pauling had published an incorrect structure of DNA, “mobilised” Watson to restart model building efforts with Crick. Important contributions and data from Wilkins, Franklin (obtained via Max Perutz) and colleagues in Cambridge enabled Watson and Crick to propose a double-helix model for DNA. The third and longest phase of Wilkins' work on DNA took place from 1953 onwards. Here Wilkins led a major project at King's College, London, to test, verify and make significant corrections to the DNA model proposed by Watson and Crick and to study the structure of RNA. Wilkins, Crick and Watson were awarded the 1962 Nobel Prize for Physiology or Medicine, ""for their discoveries concerning the molecular structure of nucleic acids and its significance for information transfer in living material.""