DNA Translocation Through Nanopores
... Left: Small unilamellar vesicles (SUVs) get in contact with the silicon nitride membrane, burst, and merge into a lipid bilayer. Right: Fluorescence image of the lipid bilayer completely covering one side of the silicon nitride membrane. The round flake in the center indicates that the lipid bilayer ...
... Left: Small unilamellar vesicles (SUVs) get in contact with the silicon nitride membrane, burst, and merge into a lipid bilayer. Right: Fluorescence image of the lipid bilayer completely covering one side of the silicon nitride membrane. The round flake in the center indicates that the lipid bilayer ...
Transcription 12.06.21 lec
... Transcription. Here's a strand of DNA that gets split apart; then there's the initial copying of that chain. Transcription is where you're just making pieces of RNA from DNA. Translation's where you act ...
... Transcription. Here's a strand of DNA that gets split apart; then there's the initial copying of that chain. Transcription is where you're just making pieces of RNA from DNA. Translation's where you act ...
Sbjct = Alu sequence
... in vivo in the cell each time DNA is replicated prior to cell division. However there are some important differences. First, denaturing (separating) DNA is accomplished by heating the sample rather than by enzymatic activity (helicase), as it is accomplished in the cell. Second DNA primers are used ...
... in vivo in the cell each time DNA is replicated prior to cell division. However there are some important differences. First, denaturing (separating) DNA is accomplished by heating the sample rather than by enzymatic activity (helicase), as it is accomplished in the cell. Second DNA primers are used ...
Genomic Digital Signal Processing
... peak at frequency 2π/3 in their Fourier spectra. This is called the period-3 property (see Tiwari et al. [4]). The period-3 property is related to the different statistical distributions of codons between protein-coding and noncoding DNA sections. The period-3 property can be used as a basis for ide ...
... peak at frequency 2π/3 in their Fourier spectra. This is called the period-3 property (see Tiwari et al. [4]). The period-3 property is related to the different statistical distributions of codons between protein-coding and noncoding DNA sections. The period-3 property can be used as a basis for ide ...
Note 8.1 - Cloning DNA
... Restriction enzymes were first discovered by DR. Hamilton Smith in 1970, as he studied bacteria and their ability to resisted viral infections. Since the start of his research there have been 2500 restriction enzymes classified with a 200 target sequences. The primary functions of restriction enzyme ...
... Restriction enzymes were first discovered by DR. Hamilton Smith in 1970, as he studied bacteria and their ability to resisted viral infections. Since the start of his research there have been 2500 restriction enzymes classified with a 200 target sequences. The primary functions of restriction enzyme ...
Polymerase Chain Reaction (PCR)
... father. The genes are amplified using PCR, and then run through electrophoresis. The position of the two bands on the electrophoresis gel depends on the exact number of repeats at the locus. ...
... father. The genes are amplified using PCR, and then run through electrophoresis. The position of the two bands on the electrophoresis gel depends on the exact number of repeats at the locus. ...
VII. Molecular Biology Techniques
... Northern blots allow investigators to determine the molecular weight of an mRNA and to measure relative amounts of the mRNA present in different samples. RNA (either total RNA or just mRNA) is separated by gel electrophoresis, usually an agarose gel. Because there are so many different RNA molecules ...
... Northern blots allow investigators to determine the molecular weight of an mRNA and to measure relative amounts of the mRNA present in different samples. RNA (either total RNA or just mRNA) is separated by gel electrophoresis, usually an agarose gel. Because there are so many different RNA molecules ...
end of semester main examination
... Where, approximately, will the start site of transcription be? [3 Marks] ...
... Where, approximately, will the start site of transcription be? [3 Marks] ...
DNA replication
... What is a replication fork and how many are there? Why are single-stranded binding (SSB) proteins required? How does synthesis differ on leading strand and lagging strand? Which is continuous and semi-discontinuous? What are Okazaki fragments? How do polymerase I and III differ? ...
... What is a replication fork and how many are there? Why are single-stranded binding (SSB) proteins required? How does synthesis differ on leading strand and lagging strand? Which is continuous and semi-discontinuous? What are Okazaki fragments? How do polymerase I and III differ? ...
Alternative storing of DNA and biological samples using chitosan
... degradation and without any problems to use DNA and biological samples for next analysis, and last but not least, a range of samples should be stored in a limited space. A lot of inventions relate to DNA storing methods with the aim to preserve it in a stabilized state at room temperature for an ext ...
... degradation and without any problems to use DNA and biological samples for next analysis, and last but not least, a range of samples should be stored in a limited space. A lot of inventions relate to DNA storing methods with the aim to preserve it in a stabilized state at room temperature for an ext ...
Maurice Wilkins
Maurice Hugh Frederick Wilkins CBE FRS (15 December 1916 – 5 October 2004) was a New Zealand-born English physicist and molecular biologist, and Nobel Laureate whose research contributed to the scientific understanding of phosphorescence, isotope separation, optical microscopy and X-ray diffraction, and to the development of radar. He is best known for his work at King's College, London on the structure of DNA which falls into three distinct phases. The first was in 1948–50 where his initial studies produced the first clear X-ray images of DNA which he presented at a conference in Naples in 1951 attended by James Watson. During the second phase of work (1951–52) he produced clear ""B form"" ""X"" shaped images from squid sperm which he sent to James Watson and Francis Crick causing Watson to write ""Wilkins... has obtained extremely excellent X-ray diffraction photographs""[of DNA]. Throughout this period Wilkins was consistent in his belief that DNA was helical even when Rosalind Franklin expressed strong views to the contrary.In 1953 Franklin instructed Raymond Gosling to give Wilkins, without condition, a high quality image of ""B"" form DNA which she had unexpectedly produced months earlier but had “put it aside” to concentrate on other work. Wilkins, having checked that he was free to personally use the photograph to confirm his earlier results, showed it to Watson without the consent of Rosalind Franklin. This image, along with the knowledge that Linus Pauling had published an incorrect structure of DNA, “mobilised” Watson to restart model building efforts with Crick. Important contributions and data from Wilkins, Franklin (obtained via Max Perutz) and colleagues in Cambridge enabled Watson and Crick to propose a double-helix model for DNA. The third and longest phase of Wilkins' work on DNA took place from 1953 onwards. Here Wilkins led a major project at King's College, London, to test, verify and make significant corrections to the DNA model proposed by Watson and Crick and to study the structure of RNA. Wilkins, Crick and Watson were awarded the 1962 Nobel Prize for Physiology or Medicine, ""for their discoveries concerning the molecular structure of nucleic acids and its significance for information transfer in living material.""