DNA Ladder, Supercoiled (D5292) - Datasheet - Sigma
... Notes: 1. For best results load 10 times more supercoiled ladder than sample so that the ladder and sample stain at similar intensity. 2. Migration rates of supercoiled DNA plasmids vary greatly with agarose concentration, running buffer, and voltage. AH,RS,PHC 08/10-1 ...
... Notes: 1. For best results load 10 times more supercoiled ladder than sample so that the ladder and sample stain at similar intensity. 2. Migration rates of supercoiled DNA plasmids vary greatly with agarose concentration, running buffer, and voltage. AH,RS,PHC 08/10-1 ...
PicoPure DNA Extraction Kit
... samples. DNA was extracted using the PicoPure DNA Extraction Kit; a single-copy 536 bp human β-globin gene fragment was amplified from each sample using standard protocols, and the products were electrophoresed on a 10% polyacrylamide/TBE gel and stained with Invitrogen™ SYBR™ Gold Nucleic Acid Stai ...
... samples. DNA was extracted using the PicoPure DNA Extraction Kit; a single-copy 536 bp human β-globin gene fragment was amplified from each sample using standard protocols, and the products were electrophoresed on a 10% polyacrylamide/TBE gel and stained with Invitrogen™ SYBR™ Gold Nucleic Acid Stai ...
DNA and Gene Expression - Zanichelli online per la scuola
... were separated—the bacteria cells were labeled with 32P, indicating that DNA had entered the cells. ...
... were separated—the bacteria cells were labeled with 32P, indicating that DNA had entered the cells. ...
Document
... How do SNPs relate to performance? • The cattle genome has ~ 3 billion nucleotides • Scientists have discovered ~ 40 million SNPs ...
... How do SNPs relate to performance? • The cattle genome has ~ 3 billion nucleotides • Scientists have discovered ~ 40 million SNPs ...
Biotechnology 2
... blood sample 1 from crime scene blood sample 2 from crime scene blood sample 3 from crime scene “standard” blood sample from suspect ...
... blood sample 1 from crime scene blood sample 2 from crime scene blood sample 3 from crime scene “standard” blood sample from suspect ...
Replication The Cell Cycle Cell Cycle Cartoon Replication Occurs
... • Since 3’-5’ exo leaves 3’OH product on primer, 5’dNTP may be added to proofread • Therefore, the requirement for a 5’→3’ direction is necessary for proofreading. ...
... • Since 3’-5’ exo leaves 3’OH product on primer, 5’dNTP may be added to proofread • Therefore, the requirement for a 5’→3’ direction is necessary for proofreading. ...
BIOCHEMISTRY Nucleic Acids
... By convention, the sequence of bases on a NA strand (DNA or RNA) is read from the 5’ end to the 3’ end. ...
... By convention, the sequence of bases on a NA strand (DNA or RNA) is read from the 5’ end to the 3’ end. ...
More Basic Biotechnology Tools Many uses of restriction enzymes
... blood sample 1 from crime scene blood sample 2 from crime scene blood sample 3 from crime scene “standard” blood sample from suspect ...
... blood sample 1 from crime scene blood sample 2 from crime scene blood sample 3 from crime scene “standard” blood sample from suspect ...
Supplementary Notes - Word file (74 KB )
... terminated with a 3’-dideoxy residue. All oligos were purchased from Sigma and purified by denaturing gel electrophoresis and elution. For the annealing, 1 µg of oligos 1 and 3 were annealed with 2 µg oligo 2. The resulting nicked duplex was purified by neutral PAGE and treated with 100 nM T4 DNA li ...
... terminated with a 3’-dideoxy residue. All oligos were purchased from Sigma and purified by denaturing gel electrophoresis and elution. For the annealing, 1 µg of oligos 1 and 3 were annealed with 2 µg oligo 2. The resulting nicked duplex was purified by neutral PAGE and treated with 100 nM T4 DNA li ...
Forensic Science – Study Guide for Final (Spring 2013)
... Forensic Science a. Definition b. Scientific Method c. Components of a lab report; Conclusions Evidence: a. Differentiate between class vs. individual; direct vs. circumstantial; b. What is trace evidence? Provide examples and their use. c. Differentiate between direct transfer and secondary transfe ...
... Forensic Science a. Definition b. Scientific Method c. Components of a lab report; Conclusions Evidence: a. Differentiate between class vs. individual; direct vs. circumstantial; b. What is trace evidence? Provide examples and their use. c. Differentiate between direct transfer and secondary transfe ...
Cell Cycle PowerPoint
... • Cell division is the process by smaller which a parent cell divides into two or more daughter cells (IDENTICAL CELLS!). ...
... • Cell division is the process by smaller which a parent cell divides into two or more daughter cells (IDENTICAL CELLS!). ...
Maurice Wilkins
Maurice Hugh Frederick Wilkins CBE FRS (15 December 1916 – 5 October 2004) was a New Zealand-born English physicist and molecular biologist, and Nobel Laureate whose research contributed to the scientific understanding of phosphorescence, isotope separation, optical microscopy and X-ray diffraction, and to the development of radar. He is best known for his work at King's College, London on the structure of DNA which falls into three distinct phases. The first was in 1948–50 where his initial studies produced the first clear X-ray images of DNA which he presented at a conference in Naples in 1951 attended by James Watson. During the second phase of work (1951–52) he produced clear ""B form"" ""X"" shaped images from squid sperm which he sent to James Watson and Francis Crick causing Watson to write ""Wilkins... has obtained extremely excellent X-ray diffraction photographs""[of DNA]. Throughout this period Wilkins was consistent in his belief that DNA was helical even when Rosalind Franklin expressed strong views to the contrary.In 1953 Franklin instructed Raymond Gosling to give Wilkins, without condition, a high quality image of ""B"" form DNA which she had unexpectedly produced months earlier but had “put it aside” to concentrate on other work. Wilkins, having checked that he was free to personally use the photograph to confirm his earlier results, showed it to Watson without the consent of Rosalind Franklin. This image, along with the knowledge that Linus Pauling had published an incorrect structure of DNA, “mobilised” Watson to restart model building efforts with Crick. Important contributions and data from Wilkins, Franklin (obtained via Max Perutz) and colleagues in Cambridge enabled Watson and Crick to propose a double-helix model for DNA. The third and longest phase of Wilkins' work on DNA took place from 1953 onwards. Here Wilkins led a major project at King's College, London, to test, verify and make significant corrections to the DNA model proposed by Watson and Crick and to study the structure of RNA. Wilkins, Crick and Watson were awarded the 1962 Nobel Prize for Physiology or Medicine, ""for their discoveries concerning the molecular structure of nucleic acids and its significance for information transfer in living material.""